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Journal : Journal of Fisheries

Implementation of 16S rRNA Gene for Fish and Shrimp Barcoding in Mangrove Ecosystems in North Sumatra and Aceh, Indonesia Hatika Hairani; Rizka Amelia; Ipanna Enggar Susetya; Arida Susilowati; Yuntha Bimantara; Tadashi Kajita; Mohammad Basyuni
Jurnal Ilmiah Perikanan dan Kelautan Vol. 15 No. 1 (2023): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v15i1.36686

Abstract

Highlight Research The genetic diversity of fish and shrimp from mangrove habitats was relatively substantial, with a heterozygosity of 0.926 and the exception of Langsa samples. AMOVA revealed that the diversity among individuals in the populations were higher compared to those within individuals. DNA barcode in mangroves is useful in developing mangrove conservation and restoration initiatives   Abstract Fish and shrimp are among the species that are vulnerable to high level of disturbance in mangrove ecosystem. This study aimed to investigate the implementation of 16S rRNA gene for fish and shrimp barcoding in mangrove ecosystems in North Sumatra and Aceh, Indonesia. 50 samples fresh fish and shrimp were collected from five mangrove ecosystems in North Sumatra, namely Belawan, Lubuk Kertang, Percut Sei Tuan, and Pulau Sembilan and Langsa of Aceh for DNA extraction process. The research was divided into four activities: sample collection, DNA isolation, PCR amplification, and data analysis. DNA was extracted using kit extraction (kit Reliaprep gDNA Tissue Miniprep System) and with PCR amplification. Genetic parameters were analyzed using Gen Alex 6.51 software and the relationship between sites was examined using the MVSP 3.22. The genetic diversity of fish and shrimp from mangrove habitats was relatively substantial, according to the findings, with a heterozygosity of 0.926, with the exception of Langsa samples, which were only identified in subclass A1. Genetic relationship between sites revealed that all samples clustered into two branches and were randomly dispersed within each site. This work confirmed the 16S rRNA gene worked for fish and shrimp barcoding in mangrove ecosystems, North Sumatra and Aceh, Indonesia and our findings are expected to be useful in developing mangrove conservation and restoration initiatives.
Mollusk Diversity in Percut Sei Tuan Silvofishery Ponds, North Sumatra, Indonesia Ipanna Enggar Susetya; Mohammad Basyuni; Muhammad Rakezza Rifzy Rifzy; Rizka Amelia; Yuntha Bimantara; Rusdi Leidonald; Amanatul Fadhilah
Jurnal Ilmiah Perikanan dan Kelautan Vol. 15 No. 2 (2023): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.vi.37772

Abstract

Highlight Research We found 16 species of mollusks with a total of 15 species from the gastropod class and 1 species from the Bivalvia class bivalves in the silvofishery ponds, recording a total of 369 individuals. The density of mollusks in July in pond I was 3.6 ind/m2, while it was 10.66 ind/m2 in pond II. The mollusk diversity index in October after rehabilitation was included in the high diversity category because the value of H' was 1.6 - 3.0. Pond I has a diversity index value of 1.89 and pond II 1.82. The value of C-organic concentration in both ponds ranged from 3.00% to 5.72%, which is the optimum level for mollusk growth.   Abstract Silvofishery is a system that combines mangrove and fishery to protect mangrove ecosystems and restore their functions, one of which is as a habitat for macrozoobenthos. Investigate the mollusks and mangrove density. Mollusk sampling was carried out on sub-plots measuring 1 x 1 m² in the mangrove vegetation transect, and mangrove density was measured by counting the number of trees directly on all of the mangrove species at two silvofishery ponds. A total of 16 species of mollusks were found at the observation site, consisting of gastropods and bivalves. The density of mollusks in July was 20 ind/m2 in pond I, while it recorded 9 ind/m2 in pond II. In October, after pond II rehabilitation, the density was inversely proportional to July, and the highest density was found in pond I at 10 ind/m2, while pond II measured 9 ind/m2. After rehabilitation, the index value H' decreased in pond I and pond II from 2.82 and 2.98 to 2.52 and 2.68, respectively. The mangrove density was 887 ind/ha in pond I and 686 ind/ha in pond II. The substrate in both ponds was clay. Nitrate concentration values "‹"‹in the substrate in both ponds ranged from 0.27% to 0.29%, phosphate recorded a percentage of 0.07–0.09%, and C-organic ranged from 3.00% to 5.72%. The water's physicochemical parameters and the type and content of the substrate were still suitable for mollusk life.
Co-Authors . Delvian . Rosmayati Abdi Wira Septama Abu Hayullah Ade Khana Saputri Adil Junaidi Ahmad Baiquni Rangkuti Ahmad Muhtadi Aisar Novita Al Mustaniroh, Shofiyah Sabilah Alfian Mubaraq Alfredyanto Sianturi Ameilia Zuliyanti Siregar Ameilia Zuliyanti Siregar Amir, Ahmad Aldrie Andi Aznan Aznawi Andi Hermawan Andriyani, Yunilda Anggita Amelati Harahap Ani Suryanti Apri Heri Iswanto Apriliyani Apriliyani Arida Susilowati Iswanto Arif Nuryawan Arif NURYAWAN Arrasyid, Nurfida Khairina Aznawi , Andi Aznan Aznawi, Andi Aznan Bejo Slamet Bejo Slamet Berliana Nainggolan Bora Jeong BUDI UTOMO Cemda, Abdul Rahman Delvian Delvian Deni Arifiyanto Deni Elfiati Deni Elfiati Desrita Desrita Desrita Desrita Desya Alvionita Keliat Dewinta , Astrid Fauzia Diana Sofia Hanafiah DWI SURYANTO Edison Purba Eko Budiyulianto Elfiati, Deni Elfiati Elham Sumarga, Elham Epifani Natalia Gultom Erman Munir Erni Jumilawaty Erni Jumilawaty etti sartina siregar Etti Sartina Siregar Evan Kharogi Sinulingga Fadhilah, Amanatul Hamsyah Rianda Harahap Hamzah, Hamzah Hardiansyah Tambunan Harisyah Manurung Hasanuddin, Aam Hasbi Nurainun Hasbi Nurainun Nurainun Hasibuan , Julia Syahriani Hatika Hairani Heru Prayogi Hiroshi Sagami Hiroshi Sagami Hirosuke Oku Hirosuke Oku Hirosuke Oku Hirosuke Oku Indra Lesmana Indra Syahputra Ipanna Enggar Susetya Ipanna Enggar Susetya Irvan , Irvan Iwan Risnasari Iwan RISNASARI Jayusman Jayusman Jeprianto Manurung Joshi, Ravindra C. Julayha . Julayha Julayha Julia Syahriani Hsb Jumilawaty, Erni Jumilawaty Kiking Ritarwan Koko Tampubolon Latifa Sari Dalimunthe Latifah Nur Siregar Leopardas, Venus Leopardas, Venus E Lollie A.P. Putri Lollie A.P. Putri Lollie Agustina Lollie Agustina Pancawaraswati Putri Lollie Agustina Pancawaraswati Putri Lollie Agustina Pancawaraswati Putri Lollie AP Putri Lollie AP. Putri M. Luthfi Dharmawan Mariah Ulfa Mariany Siagian Marnida Uli Lubis Masfria Masfria Melinda Sari Lubis Merlyn Mariana Meutia Nauly Monalia Hutauruk Muammar Bakar Murni Mubaraq , Alfian Mubaraq, Alfian Mubaraq, Alfian Mubaraq Muhammad Rakezza Rifzy Rifzy Mukti Batubara Mulyar Hamka Muttia Chandraputri Rizkiany Nasution, Melvi Wahyu Angraini Nauly, Meutia Nauly Nini Rahmawati Nofrizal Amri Nofrizal Amri Nur Indah Lestari Nurdin Sulistiyono Nurfalah Siregar Oding Affandi Oke Rina Ramayani, Oke Rina Prayunita Prayunita Prita Yulianti Anasta br G Purwoko, Agus Putri Ester Sihaloho Putri Permata Sari Sirait Putri, Lollie Lollie Qalbi, Mutiara Putri Rachel Nababan Rahmawaty Rahmawaty Rangkuti, Ahmad Baiquni Ridha Wati Ridwanti Batubara Risnasari, Iwan Risnasari Ritonga, Lia Anggraini Rizka Amelia Rizka Amelia Rizka Amelia Rusdi Leidonald Ruth Melisa Ryzki Pebriansyah San France Sarah Sarah, Sarah Shigeyuki Baba Silvia Nora, Silvia Sim, Yenti Siregar, Lutfhi Aziz Mahmud Siregar, Netti Herlina SITI HALIMAH LAREKENG, SITI HALIMAH Sivaipram , Itchika Sivaipram, Itchika Sivaipram, Itchika Sivaipram Slamet, Bejo Slamet Suci Rahayu Suci Rahayu Rahayu Sulistyono, Nurdin Sumardi Sumardi Susilowati, Arida Susilowati Syahputra, OK Hasnanda Tadashi Kajita Tadashi Kajita Tansy, Belinda Calista Tati Suryati Syamsudin Try Miharza Ulfah Juniarti Siregar Utomo, Budi WINDRA PRIAWANDIPUTRA, WINDRA Yunasfi Yunasfi Yunasfi yunasfi yunasfi Yunasfi, Yunasfi Yuntha Bimantara Yuntha Bimantara Yuntha Bimantara Yuntha Bimantara Zulham Apandy