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Antimicrobe activity of empty bunches of palm tree’s liquid smoke against Sclerotium rolfsii Sacc. and Puccinia arachidis Speg. 1884 Pratiwi, Rizky Nomi; Tondok, Efi Toding; Damayanti, Tri Asmira
Agrovigor Vol 18, No 1 (2025)
Publisher : Universitas Trunojoyo Madura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21107/agrovigor.v18i1.29632

Abstract

Groundnut (Arachis hypogaea L.) is one of the food crops that has high economic value. However, one of the constraints to groundnut production is the stem base rot (Sclerotium rolfsii) and rust (Puccinia arachidis) diseases. Liquid smoke is known to have antimicrobial activities that need to be further studied for their potential to control various types of pathogens. This study aimed to test the ability of liquid smoke from empty palm bunches in controlling S. rolfsii and P. arachidis in vitro. The phytotoxicity of liquid smoke to seeds was tested using a rolled paper test established in plastic. Antimicrobial activity was tested by using the poisoning medium method against S. rolfsii, while spore germination test against P. arachidis was conducted by direct liquid smoke dripping onto uredospore on object glass. Antimicrobial activity of liquid smoke at concentrations of 0.1%, 0.5%, 1.0%, 1.2%, and 1.4% showed growth inhibition of S. rolfsii by 2.0%, 5.4%, 35.0%, 78.8%, and 100%, respectively. At 24 hours after treatment, the effect of liquid smoke on P. arachidis spore germination at concentrations of 0.1% and 1.0% was 1.7% and 0.0% when compared to the control, 8.4%, and at 48 hours after treatment was 1.5% and 0.0% compared to the control, 9.7%. It indicates the ability of liquid smoke as an antifungal. 
Fluorescence Imaging as a Non-Destructive Method for Aflatoxin Detection in Corn Kernels: Recent Advances and Challenges Nofiyanti, Sri Handayani; Ahmad, Usman; Tondok, Efi Toding; Widodo, Slamet
Jurnal Teknik Pertanian Lampung (Journal of Agricultural Engineering) Vol. 14 No. 2 (2025): April 2025
Publisher : The University of Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jtep-l.v14i2.714-731

Abstract

Fluorescence imaging has developed as a promising non-invasive method for identifying aflatoxin contamination in agricultural commodities, especially corn kernels. This paper examines current improvements in fluorescence imaging technologies, highlighting its potential to improve food safety through swift and precise detection of mycotoxins. The paper examines the basics of fluorescence, the necessary setup for optimal imaging, and the issues related to background fluorescence interference, sensitivity, and the construction of calibration models. Although there are some limitations, fluorescence imaging presents considerable advantages, such as cost-efficiency and the capacity to obtain concurrent spectral and spatial data. Proposed future research objectives include the validation of imaging systems using naturally contaminated samples, the optimization of imaging parameters, and the integration of machine learning techniques to enhance data processing. By overcoming existing constraints and utilizing technical progress, fluorescence imaging can serve as an essential instrument in the detection of aflatoxin contamination, hence enhancing food safety. Keywords: Aflatoxin, Detection, Fluorescence imaging, Food safety, Machine learning.
Black pod disease (BPD) of cacao caused by Phytophthora palmivora(Butl.) is one of the major diseases on cacao plantation worldwide. Endophytic fungi (EF), fungi that live asymptomatically inside healthy plants, were examined to study their potentials as biocontrol agent of the disease. Six of EF selected from 37 species (from 2843 isolates), isolated from healthy pods of cacao from Marena in Central Sulawesi were tested for their abilities to control BPD. Pods on living trees in the field were Tondok, Efi Toding; Sinaga, Meity Suradji; Widodo, ,; Suhartono, Maggy Thenawidjaja
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 40 No. 2 (2012): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24831/jai.v40i2.6382

Abstract

Black pod disease (BPD) of cacao caused by Phytophthora palmivora(Butl.) is one of the major diseases on cacao plantation worldwide. Endophytic fungi (EF), fungi that live asymptomatically inside healthy plants, were examined to study their potentials as biocontrol agent of the disease. Six of EF selected from 37 species (from 2843 isolates), isolated from healthy pods of cacao from Marena in Central Sulawesi were tested for their abilities to control BPD. Pods on living trees in the field were sprayed with each EF propagules. The inoculated pods were harvested two weeks later and subsequently inoculated with P. palmivora. Scoring of disease development was performed and quantified as area under disease progress curve (AUDPC). Latent period, infection rate and effectiveness were also recorded. In vitro growth inhibition of pathogen and induced plant defense mechanisms due to EF were also investigated. Xylariaceaeand Calocybe gambosatreatment generated the highest effectiveness control level, i.e. 38.8% and 33.8% respectively, followed by Resinicium friabileand Aschersoniatreatment, i.e. 17.4% dan 12.7% respectively.  Pestalotiopsisand Fusariumwere not effective to control BPD. There was a strong connection between disease severity of BPD with the latent period of pathogen. Growth inhibition of pathogen and induced resistance of plant were partially responsible for disease suppression by Xylariaceae, C. gambosa, R. friabileand Aschersonia. Keywords: Calocybe gambosa, induced resistance, peroxidases, salicylic acid, Xylariaceae
Pattern distribution and epidemic factors of stem canker disease caused by Neoscytalidium dimidiatum on pitahaya in Banyuwangi Regency, East Java, Indonesia Sukmana, Andra Sahab; Tondok, Efi Toding; Wiyono, Suryo
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 26 No. 1 (2026): MARCH, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA: JOURNAL OF TROPICAL PLANT PE
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jhptt.12646-58

Abstract

Neoscytalidium dimidiatum is an airborne pathogen that causes stem canker disease, characterized by small brown spots surrounded by a yellow halo on the plant stem. These spots expand and darken from brown to dark brown and eventually black. In addition to attacking the plant stem, this disease can also infect the fruit. This research aims to understand the distribution pattern model of stem canker disease and to identify the factors contributing to the epidemic caused by N. dimidiatum. The study was conducted through the collection of secondary data, field observations of disease severity, assessment of farmers’ cultivation practices via questionnaires, isolation of phyllosphere and rhizosphere microbes, and soil chemical analysis. The results showed that this pathogen initially exhibits a random distribution pattern but later develops into infection patches with two possible dissemination modes based on the observed distribution pattern: airborne transmission and vegetative planting materials. Environmental factors influencing the stem canker epidemic include rainfall exceeding 98 mm/month, a temperature of 27 ºC, and humidity of 78.3%. Biotically, the diversity and abundance of phylloplane and rhizosphere microbes in low-severity areas are higher than in high-severity areas. From the abiotic aspect of soil chemistry, the elements P, Mg, Fe, and total N are associated with reduced stem canker intensity. Four aspects of cultivation techniques are closely related to the human role in the formation of the stem canker epidemic: the use of phytohormones, the control methods employed, the choice of pitahaya varieties, and yield losses.
Co-Authors , Widodo . Giyanto Abdjad Asih Nawangsih Abdul Munif ABDUL MUNIF Ali Wafa Alisya Talita Papona Aloysius Rusae Ana Septiana Saputri Andika Septiana Suryaningsih Anis Mutirani Atit Kanti Atit Kanti Bonjok Istiaji Bonny Poernomo Wahyu Soekarno Bonny Poernomo Wahyu Soekarno Dede Maryana Desi Andini Desi Trisnawati Desy Nur Avifah devi ayu komalaningrat Diny Dinarti Dwi Andini Shiami Eka Wijayanti Evan P. Ramdan Fitri Kemala Sandra Fitrianingrum Kurniawati, Fitrianingrum Giyanto Giyanto Giyanto Hagia Sophia Khairani Heriyanto Syafutra Hermanu Triwidodo I Made Sudiana I Made Sudiana I Nyoman Sumerta Idris Idris Irwanto Sucipto Jean Nihana Manalu Kartini Budiastuti Kikin H Mutaqin Kustiariyah Tarman Kusumah, Yayi Munara Lilik Pujantoro Eko Nugroho Lilik Retnowati Maggy Thenawidjaja Suhartono Marianah, Lisa Masrukhin Masrukhin Meity Suradji Sinaga Meity Suradji Sinaga Meity Suradji Sinaga MEITY SURADJI SINAGA Melli Fitriani nFN Khaerati Nofiyanti, Sri Handayani Nurholis Nurholis Papona, Alisya Talita Parlindo, Fitra Paulus Woyen Pratiwi, Rizky Nomi Ramdan, Evan Purnama Rika Estria Gurusinga Rizky G S Purnama Sari, Rahmah Dian Sa’adah, Rima Nur Halimatu Siti Juariyah Slamet Widodo Slamet Widodo SRI HENDRASTUTI HIDAYAT Sukmana, Andra Sahab Supramana Surono Surono Suryo Wiyono Syam, Nur Asmasari Tanjung, Mei Rani Toga Pangihotan Napitupulu TRI ASMIRA DAMAYANTI Usman Ahmad Wawan Setiawan Widodo Widodo Widodo Widodo Widodo Widodo Yadi Suryadi Yayu Siti Nurhasanah Yunus Effendi