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Black pod disease (BPD) of cacao caused by Phytophthora palmivora(Butl.) is one of the major diseases on cacao plantation worldwide. Endophytic fungi (EF), fungi that live asymptomatically inside healthy plants, were examined to study their potentials as biocontrol agent of the disease. Six of EF selected from 37 species (from 2843 isolates), isolated from healthy pods of cacao from Marena in Central Sulawesi were tested for their abilities to control BPD. Pods on living trees in the field were Tondok, Efi Toding; Sinaga, Meity Suradji; Widodo, ,; Suhartono, Maggy Thenawidjaja
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 40 No. 2 (2012): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24831/jai.v40i2.6382

Abstract

Black pod disease (BPD) of cacao caused by Phytophthora palmivora(Butl.) is one of the major diseases on cacao plantation worldwide. Endophytic fungi (EF), fungi that live asymptomatically inside healthy plants, were examined to study their potentials as biocontrol agent of the disease. Six of EF selected from 37 species (from 2843 isolates), isolated from healthy pods of cacao from Marena in Central Sulawesi were tested for their abilities to control BPD. Pods on living trees in the field were sprayed with each EF propagules. The inoculated pods were harvested two weeks later and subsequently inoculated with P. palmivora. Scoring of disease development was performed and quantified as area under disease progress curve (AUDPC). Latent period, infection rate and effectiveness were also recorded. In vitro growth inhibition of pathogen and induced plant defense mechanisms due to EF were also investigated. Xylariaceaeand Calocybe gambosatreatment generated the highest effectiveness control level, i.e. 38.8% and 33.8% respectively, followed by Resinicium friabileand Aschersoniatreatment, i.e. 17.4% dan 12.7% respectively.  Pestalotiopsisand Fusariumwere not effective to control BPD. There was a strong connection between disease severity of BPD with the latent period of pathogen. Growth inhibition of pathogen and induced resistance of plant were partially responsible for disease suppression by Xylariaceae, C. gambosa, R. friabileand Aschersonia. Keywords: Calocybe gambosa, induced resistance, peroxidases, salicylic acid, Xylariaceae
Potensi Aktinomiset Filoplan Asal Tumbuhan Solanaceae sebagai Agens Pengendali Hayati Phytophthora capsici pada Cabai: The Potential of Phyllospheric Actinomycetes from Solanaceae Plants as Biocontrol Agents of Phytophthora capsici on Chili Pepper Basri, Muhamad; Tondok, Efi Toding; Giyanto
Jurnal Fitopatologi Indonesia Vol. 21 No. 1 (2025): Maret 2025
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14692/jfi.21.1.64-77

Abstract

The Potential of Phyllospheric Actinomycetes from Solanaceae Plants as Biocontrol Agents of Phytophthora capsici on Chili Pepper Chili rot is an important disease caused by an Oomycete species, Phytophthora capsici. This fungus infects chili plants with a potential yield loss of more than 80%. The use of actinomycetes as a biocontrol agent is an environmentally friendly approach that effectively suppresses the growth of P. capsici through the production of antibiotics, lytic enzymes, and competition for space and nutrients. This study aimed to obtain the best actinomycetes isolated from Solanaceae plants that could suppress the growth of P. capsici. This research included isolation of actinomycetes, biosafety assay, antagonistic potential assay, analytic hierarchy process, and molecular identification. There were 71 actinomycetes isolated from 11 plant species in the the Solanaceae family. Actinomycetes isolates that passed the biosafety assay were 39 isolates. The results of antagonistic potential screening revealed 16 actinomycetes isolates capable of suppressing the growth of P. capsici. A dual culture assay showed that actinomycetes isolates were able to inhibit the growth of P. capsici in petri dishes up to 98.2% by isolate 46PPS. Furthermore, in volatile organic compounds assay, the growth of P. capsici was reduced up to 74.1% by isolate 41LAL. While filtrate of actinomycetes isolate 25TPT inhibited growth of P. capsici in liquid medium by up to 98.2%. The cellulolytic index showedthe ability of actinomycetes to break down cellulose molecules, with a cellulase index value of 2.1 by isolate 2LAP. Scoring with the AHP method narrowed down 16 isolates into four best candidates with isolate codes 2LAP, 25TPT, 37LAT, and 41LAL. The results of molecular identification showed that the four selected isolates were Streptomyces pratensis, S. seoulensis, Pseudonocardia tropica, and S. somaliensis.
Fluorescence Imaging as a Non-Destructive Method for Aflatoxin Detection in Corn Kernels: Recent Advances and Challenges Sri Handayani Nofiyanti; Usman Ahmad; Efi Toding Tondok; Slamet Widodo
Jurnal Teknik Pertanian Lampung (Journal of Agricultural Engineering) Vol. 14 No. 2 (2025): April 2025
Publisher : The University of Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jtep-l.v14i2.714-731

Abstract

Fluorescence imaging has developed as a promising non-invasive method for identifying aflatoxin contamination in agricultural commodities, especially corn kernels. This paper examines current improvements in fluorescence imaging technologies, highlighting its potential to improve food safety through swift and precise detection of mycotoxins. The paper examines the basics of fluorescence, the necessary setup for optimal imaging, and the issues related to background fluorescence interference, sensitivity, and the construction of calibration models. Although there are some limitations, fluorescence imaging presents considerable advantages, such as cost-efficiency and the capacity to obtain concurrent spectral and spatial data. Proposed future research objectives include the validation of imaging systems using naturally contaminated samples, the optimization of imaging parameters, and the integration of machine learning techniques to enhance data processing. By overcoming existing constraints and utilizing technical progress, fluorescence imaging can serve as an essential instrument in the detection of aflatoxin contamination, hence enhancing food safety. Keywords: Aflatoxin, Detection, Fluorescence imaging, Food safety, Machine learning.
Efektivitas Gelombang Ultrasonik dalam Eliminasi Nematoda Daun Aphelenchoides pada Umbi Bawang Merah Kurniawati, Fitrianingrum; Supramana, Supramana; Hidayat, Sri Hendrastuti; Tondok, Efi Toding; Syafutra, Heriyanto; Damayanti, Tri Asmira; Mubin, Nadzirum
Jurnal Agrikultura Vol 37, No 1 (2026): April, 2026
Publisher : Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/agrikultura.v37i1.68192

Abstract

Nematoda daun Aphelenchoides merupakan salah satu patogen tanaman yang memiliki kisaran inang yang luas dan menyebabkan kehilangan hasil panen. Metode konvensional telah sering digunakan dalam pengendalian nematoda termasuk nematisida kimia, perawatan air panas, dan nematisida hayati tetapi memiliki keterbatasan. Hal ini memerlukan eksplorasi metode alternatif yang ramah lingkungan dan efektif, seperti teknologi gelombang ultrasonik. Penelitian bertujuan mengetahui efektivitas gelombang ultrasonik dalam eliminasi nematoda daun Aphelenchoides pada umbi bawang serta pengaruh pada pertumbuhan umbi bawang. Ekstraksi nematoda menggunakan metode pengabutan (mist chamber) dan perendaman air dingin. Nematoda diidentifikasi berdasarkan karakter morfologi. Umbi bawang merah yang telah diinfestasikan Aphelenchoides diberi perlakuan ultrasonik dengan lama perlakuan selama 26, 30, 37, 44, 60 menit dan kontrol. Umbi bawang yang telah diberi perlakuan ultrasonik ditanam di pot dan diamati parameter pertumbuhannya sampai panen. Data ditabulasikan di Microsoft Excel dan diolah menggunakan SAS JMP. Perlakuan ultrasonik dengan durasi yang lebih dari 37 menit menghasilkan tingkat mortalitas yang tinggi (≥84,00%).  Waktu papar yang berbeda menunjukkan pengaruh yang tidak berbeda nyata dengan kontrol pada tinggi tanaman dan panjang akar. Lebih lanjut, perlakuan ultrasonik dengan durasi yang lebih lama (≥ 37 menit) sangat efektif dalam menekan populasi nematoda Aphelenchoides tanpa memengaruhi pertumbuhan tanaman bawang merah.
Endophytic Fungi of Dragon Fruits: Diversity and Its Potential as Biocontrol Agents of Stem Canker Disease Neoscytalidium dimidiatum Nugraha, Andhika Vidian; Giyanto, Giyanto; Tondok, Efi Toding
AGRIVITA Journal of Agricultural Science Vol 48, No 2 (2026)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v48i2.4959

Abstract

Endophytic fungi have the potential for antagonistic activity against plant pathogens and can be utilized to prevent the decline in dragon fruit production caused by stem canker disease. This study aims to assess the diversity of endophytic fungi in highland and lowland areas and to evaluate their potential against dragon fruit stem canker disease. Research has been conducted using several methods, i.e., isolation of endophytic fungi from dragon fruit, biosafety assay, potential assay, and semi-in vivo assay. Banyuwangi and Bukittinggi have moderate diversity, relatively even species distributions, and low-to-moderate dominance of particular isolates. Isolates Trichoderma sp. C6 and Trichoderma sp. C13 are able to inhibit the development of N. dimidiatum on wounded cladodes. Molecular identification showed that Trichoderma sp. C13 has a close phylogenetic relationship with T. hamatum T311. Endophytic fungi from dragon fruit plants may serve as potential biocontrol agents against N. dimidiatum. However, the mechanism by which these endophytic fungi induce resistance in dragon fruit plants against N. dimidiatum remains unclear and warrants further investigation.