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Keefektifan Asap Cair dan Elektroterapi untuk Mengeliminasi Infeksi Xanthomonas oryzae pv. oryzae pada Benih Padi Rizky G S Purnama; Kikin Hamzah Mutaqin; Efi Toding Tondok
Jurnal Fitopatologi Indonesia Vol. 14 No. 2 (2018)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (380.313 KB) | DOI: 10.14692/jfi.14.2.54

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The Effectiveness of Liquid Smoke and Electrotherapy to Eliminate Infection of Xanthomonas oryzae pv. oryzae in Rice SeedBacterial leaf blight caused by Xanthomonas oryzae pv. oryzae is a seed-borne disease that can reduce the productivity of rice. Alternative treatments that have not been widely developed is application of liquid smoke and electrotherapy. The aim of this research was to study the effectiveness of liquid smoke and electrotherapy and their combinations to eliminate X. oryzae pv. oryzae without damaging seed quality. The study consisted of three experimental stages: (1) Liquid smoke treatment on the viability of the bacteria and rice seeds; (2) Electrotherapy treatment on the viability of bacteria and rice seeds; (3) Combination of liquid smoke and electrotherapy treatment in bacteria-infected rice seed. The results showed that liquid smoke concentration (5% for 30 minutes) was able to reduce 83.33% population of X. oryzae pv.oryzae on rice seeds with 94.33% seed vigor, 98.00% germination, and time required to achieve 50% of total seed emergence (T50) of 3.13 days. Electrotherapy treatment (400 mA for 20 minutes) was not only eliminated X. oryzae pv. oryzae on rice seeds up to 100% but also maintained seed vigor 81.67%, germination 89.33%, and T50 3.47 days. Electrotherapy treatment (400 mA for 20 minutes) followed by immersion of infected rice seeds in liquid smoke concentration (1% for 30 minutes) could reduce 94.59% bacteria population while maintaining 86.00% seed germination. Treatment of electrotherapy (400 mA for 20 minutes) followed by immersion seeds into liquid smoke concentration (5% for 30 minutes) could reduce bacterial population up to 100%, but caused phytotoxicity effect to the seedling.
Potensi Cendawan Endofit sebagai Pengendali Hayati Penyakit Busuk Pangkal Batang (Phytophthora capsici) pada Bibit Cabai Evan Purnama Ramdan; Efi Toding Tondok; Suryo Wiyono; Sri Hendrastuti Hidayat; Widodo Widodo
Jurnal Fitopatologi Indonesia Vol. 13 No. 5 (2017)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (349.297 KB) | DOI: 10.14692/jfi.13.5.161

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Stem rot disease caused by Phytophthora capsici is an important disease on chilli. Eight endophytic fungi that had been isolated and screened based on pathogenecity test were further tested for their potential as the biological control agent of the stem rot disease of chilli. The endophytic fungi suspension was applied twice during the trial. The first application was on 100 seed lot, by soaking them in 100 mL of suspension. The second application was on the 3 weeks-old chili seedlings by drenching them with 10 mL suspension per plant. The concentration of endophytic fungi  in the suspension was 2.8 × 106 cfu mL-1. The disease intensity and AUDPC value were measured for 4 weeks after the pathogen inoculation. The growth inhibition test of P. capsici  was performed in vitro and the colonization abilities of endophytic fungi were observed at 4 weeks-old chilli seedlings. Eight endophytic fungi  inhibited the growth of the P. capsici, and two of those isolates namely Penicillium strain MAG1 and Penicillium strain PAB2 showed antibiosis mechanism. Endophytic fungi has the ability more to colonize at the root (26–60%) than in the stem (20–40%). Fusarium strain MAGR1 has the highest level of endophytic colonization i.e. 60% compared to others.  Based on in vivo assay, six endophytic fungi isolates, i.e. Fusarium strain MAGR1, Penicillium strain MAG1, Penicillium strain PAB2, sterile hyphae HAJ1, sterile hyphae HAJ2, and  sterile hyphae PBG7, showed the potency to control stem rot disease with inhibition level of 25.5–35.5%
Identitas Spesies Botrytis pada Tanaman Hortikultura Di Jawa Barat, Indonesia devi ayu komalaningrat; Efi Toding Tondok; widodo widodo
Jurnal Fitopatologi Indonesia Vol 14 No 6 (2018)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1896.611 KB) | DOI: 10.14692/jfi.14.6.205

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Identity of Botrytis Species on Horticultural Crops In West Java, IndonesiaBotrytis species are economically important pathogens with a very broad host range including more than 200 horticultural crops. The identity of these fungus found  in Indonesia has not been investigated and need to be reconfirmed due to the species variations of Botrytis found worldwide. The aims of this research were to identify Botrytis species infecting crops in West Java based on its morphology and molecular characteristics, as well as its pathogenicity traits. Based on morphological characters, all 25 isolates found were identified as B. cinerea. ITS-based sequences of the 8 isolates showed 96-100% similarity to reported B. cinerea in GenBank. The phylogenetic analysis confirmed that all collected B. cinerea were grouped in the same cluster with Australia, Netherlands, and other Asian region isolates. Pathogenicity tests using strawberry fruits demonstrated that all isolates were pathogenic as indicated by grey mold symptom development; the isolates from orchid showed the highest virulence. This research is the first report confirming Botrytis cinerea identity based on morphology and molecular methods in Indonesia, and also confirmed B. cinerea as the only species of Botrytis found in West Java.
Kanker Batang: Penyakit Baru pada Kopi di Lampung Suryo Wiyono; Andika Septiana Suryaningsih; Ali Wafa; Efi Toding Tondok; Bonjok Istiaji; Hermanu Triwidodo; Widodo Widodo
Jurnal Fitopatologi Indonesia Vol 15 No 1 (2019)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1580.807 KB) | DOI: 10.14692/jfi.15.1.9

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Stem Canker: A New Disease of coffee in LampungStem cancer is a new disease that has attacked smallholder coffee plantations in Lampung since 2010. The cause of the disease was unknown. This study aims to describe the symptoms of the disease, the incidence of the disease in the affected plantation, and identify morphologically and molecularly the canker pathogens of the coffee stem canker diseases. All stages of Koch’s postulate were carried out in laboratories and greenhouses. The isolated pathogens were morphologically characterized by colony shape and color as well as the conidia shape and size. Molecular identification was carried out by using a general primer (ITS1 and ITS4) and followed by sequencing. The main symptoms of the disease are stem cancer and dieback, as well as more infecting older plants. Pathogen of the coffee stem canker disease that attacks coffee plants in Lampung has been identified as Fusarium solani which has 99% homology with F. solani KY245947.1.
Insidensi Virus dan Cendawan pada Biji dan Umbi Bawang Merah Ana Septiana Saputri; Efi Toding Tondok; Sri Hendrastuti Hidayat
Jurnal Fitopatologi Indonesia Vol 14 No 6 (2018)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (408.799 KB) | DOI: 10.14692/jfi.14.6.222

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Incidence of Viruses and Fungi on True Shallot Seed and Shallot Bulb Shallot is an important vegetable in Indonesia. Shallot farmers generally use bulbs as planting material even though there is an option to use true shallot seed (TSS). One important criteria for a good seed quality as planting material is pathogen free. Research was conducted to detect and identify viruses and fungi from TSS and shallot seed bulbs cultivars Bima, Bauji, Thailand, and Tuk-Tuk. Virus detection was carried out by DIBA (dot immunobinding assay) method using specific antiserum to OYDV, SLV, SYSV, and GCLV. Detection and identification of fungi was carried out by blotter test method and polymerase chain reaction. Infection of OYDV, GCLV, SYSV, and SLV were only detected in shallot bulbs with infection rates ranging from 66% to 100%. Four species of fungi were detected in TSS and bulbs, i.e. Aspergillus niger, A.flavus, F. solani, and Rhizopus sp; whereas F.oxysporum was only found in bulbs. All isolates of F. oxysporum was pathogenic and cause disease incidence up to 55%. Pathogenic isolate of F. oxysporum had 100% homology to those isolate from China and USA on the spesies level and 91.2% to those isolate from India and USA to the forma spesies level.
Trichoderma dan Gliocladium untuk Mengendalikan Penyakit Busuk Akar Fusarium pada Bibit Kelapa Sawit Siti Juariyah; Efi Toding Tondok; Meity Suradji Sinaga
Jurnal Fitopatologi Indonesia Vol 14 No 6 (2018)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (959.644 KB) | DOI: 10.14692/jfi.14.6.196

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Trichoderma and Gliocladium for Controling Fusarium Root Rot Disease of Oil Palm SeedlingsFusarium spp. have been reported as the causal agent of common spear rot and crown rot diseases on oil palm.  An effective strategy to control these diseases is not available yet. This research was aimed to find biocontrol agents for effective control of crown rot disease on oil palm seedlings caused by Fusarium spp. The experiment consisted of 3 parts i.e. pathogenicity test of 3 isolates of Fusarium, identification and in vitro test of biocontrol agents, and in planta test of biocontrol agents against Fusarium spp. In vitro test was done through dual culture test and test for volatile compound produced by the biocontrol agents. In planta test was conducted through inoculation of Fusarium spp. into oil palm seedlings growing on medium containing  selected biocontrol agents i.e. Trichoderma harzianum, T. virens, T. inhamatum, and Gliocladium fimbriatum. In vitro test showed that Gliocladium fimbriatum 1 and 2 were inhibited effectively the growth of Fusarium spp. on the dual culture test, whereas T. harzianum Gadingrejo 2 was inhibited effectively the growth of Fusarium spp. on volatile compound test. The application of biocontrol agents was effective to protect oil palm seedlings from Fusarium spp. infection.
Keragaman Morfologi dan Molekuler Lasiodiplodia theobromae dari Tanaman Jeruk, Kakao, Karet, Manggis, dan Pisang Fitri Kemala Sandra; Yayu Siti Nurhasanah; KIKIN MUTAQIN; Suryo Wiyono; Efi Toding Tondok
Jurnal Fitopatologi Indonesia Vol 17 No 2 (2021)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14692/jfi.17.2.58-66

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Morphological and Molecular Diversity of Lasiodiplodia theobromae Isolated from Citrus, Cocoa, Rubber, Banana and Mangosteen Plants The fungus Lasiodiplodia theobromae is an important and cosmopolitan pathogen in the tropics and subtropics. The range of host plants is very wide, including economic commodities in Indonesia, i.e. citrus, cocoa, rubber, banana and mangosteen. The intraspecies diversity of fungi isolates from those five plants from different provinces in Indonesia was observed based on the morphological characteristics and molecular markers of RAPD-PCR. The intraspecies diversity was shown from the growth rate of the vegetative growth of the colonies on PDA as a base medium and the ability to produce reproductive structures. The fungal isolates from citrus, rubber and banana were able to grow faster and produce pycnidium and conidium in both PDA and modified-WA medium, while the cocoa and mangosteen isolates grow slower and only could produce these reproductive structures in the WA medium. The diversity between isolates in L. theobromae species was indicated by the morphological difference of the reproductive structures. Young conidium (aseptate) has a length ranging from 13.5-25.7 μm, width 8.1-14.0 μm, and length/width ratio 1.5-2.2; while for mature conidium (septate) 15.4-23.6 μm long, 10.7-12.8 μm wide, length/width ratio 1.4-1.9. Although the conidium sizes between isolates showed differences, they were still within the range of size of the L. theobromae species. The profile of RAPD-PCR DNA fragments using single primers OPB-01 and OPB-07 each resulted in different numbers and sizes of DNA bands between the five isolates, thus indicating the existence of molecular diversity between isolates within the same species.
Molecular Characters of AB-FAR Gene 1 of Aphelenchoides besseyi from Five Rice Varieties Fitrianingrum Kurniawati; Efi Toding Tondok; Yayi Munara Kusumah; Abdul Munif
Jurnal Fitopatologi Indonesia Vol 17 No 3 (2021)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14692/jfi.17.3.121-130

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Aphelenchoides besseyi merupakan nematoda penyebab penyakit pucuk putih yang terbawa benih padi. Gen AB FAR-1 diketahui sebagai gen penting yang mengendalikan patogenisitas A. besseyi. Penelitian dilakukan untuk mengetahui karakter gen AB FAR-1 yang diisolasi dari nematoda yang berasal dari benih padi. Ekstraksi nematoda dilakukan dengan metode corong Baerman dari benih 5 varietas padi “Ciherang“, “Inpari Sidenuk“, “Sintanur“, “Hibrida Prima“ dan “Pak Tiwi“. Ekstraksi DNA total nematoda menggunakan metode CTAB dilanjutkan dengan amplifikasi gen AB FAR-1 menggunakan primer spesifik FAR-F1/R1 dan analisis urutan nukleotidanya. Pita DNA spesifik gen AB FAR-1 berukuran 150 pb berhasil diamplifikasi dari semua sampel nematoda. Analisis sekuen menunjukkan bahwa gen AB FAR-1 tersebut memiliki homologi tertinggi (92.5 – 100%) dengan aksesi Genbank JQ686690.1, yaitu gen AB FAR-1 A. besseyi asal Cina. Walaupun memiliki homologi yang tinggi, terdapat beberapa perbedaan nukleotida pada sampel gen AB FAR-1 A. besseyi asal “Ciherang“, “Inpari Sidenuk“ dan “Hibrida Prima“. Analisis pohon filogenetika lebih lanjut mengelompokkan gen AB FAR-1 A. besseyi menjadi 2 grup, yaitu grup 1 terdiri atas gen AB FAR-1 A. besseyi asal Cina, “Sintanur“, “Hibrida Prima“ dan “Pak Tiwi“ dan grup 2 gen AB FAR-1 A. besseyi asal “Ciherang“, dan “Inpari Sidenuk“.
Screening of Liliaceae Rhizosphere Actinomycetes as Biological Control Agents of Fusarium oxysporum f. sp. cepae Eka Wijayanti; Abdjad Asih Nawangsih; Efi Toding Tondok
Jurnal Fitopatologi Indonesia Vol 17 No 6 (2021)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14692/jfi.17.6.225-232

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Penyakit moler yang disebabkan oleh Fusarium oxysporum f. sp. cepae merupakan salah satu penyakit penting pada tanaman bawang merah. Aktinomiset memiliki potensi sebagai agens pengendali hayati F. oxysporum f. sp. cepae karena kemampuannya dalam memproduksi senyawa bioaktif. Penelitian ini bertujuan mengevaluasi dan menyeleksi isolat-isolat aktinomiset yang dapat menekan pertumbuhan F. oxysporum f. sp. cepae dan memacu pertumbuhan tanaman bawang merah. Penelitian dibagi menjadi empat tahap, yaitu: isolasi, seleksi, karakterisasi, dan identifikasi isolat aktinomiset yang potensial. Sebanyak 43 isolat berhasil diisolasi dari tanaman Liliaceae dan sebanyak 14 isolat berhasil diseleksi berdasarkan hasil uji keamanan hayati. Hasil uji antagonisme menunjukkan bahwa 14 isolat menghasilkan penghambatan terhadap F. oxysporum f. sp. cepae sebesar 3.67%–53.67%. Di antara 14 isolat tersebut, sebanyak 13 isolat mampu memproduksi enzim kitinase dengan indeks kitinolitik sebesar 0.31–1.38. Lima isolat terpilih yaitu: ABF42, ABF59, ACF45, AEF35, dan AEF45, mampu melarutkan fosfat dan memproduksi IAA dengan konsentrasi 24.82–82.88 ppm, namun hanya tiga isolat yang mampu memfiksasi nitrogen. Berdasarkan sikuen gen 16S rRNA, lima isolat tersebut berturut-turut teridentifikasi sebagai: Streptomyces rameus, S. lydicus, S. panaciradicis, S. seoulensis, dan S. fuscichromogenes.
Pengaruh Varietas dan Umur Tanaman Berbeda terhadap Jumlah Populasi dan Tingkat Serangan Hama dan Penyakit Pisang (Musa sp.) di Kabupaten Sukabumi Hermanu Triwidodo; Efi Toding Tondok; Dwi Andini Shiami
Agrikultura Vol 31, No 2 (2020): Agustus, 2020
Publisher : Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/agrikultura.v31i2.27077

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Tanaman pisang merupakan tanaman hortikultura yang buahnya banyak dikonsumsi oleh masyarakat Indonesia. Tanaman pisang biasanya ditanam di sekitar halaman rumah atau lahan campuran. Penanaman pisang dengan pola monokultur seperti di kebun PT Perkebunan Negara (PTPN) VIII dapat menyebabkan ledakan hama dan penyakit pada pisang. Penelitian ini bertujuan mengetahui jenis hama dan penyakit serta kejadian dan keparahan penyakit tanaman pisang. Penelitian dilakukan pada tiga blok di PTPN VIII Parakansalak, Sukabumi. Varietas pisang yang digunakan yaitu Barangan, Cavendis dan Mas Kirana. Setiap varietas ditentukan lima rumpun secara diagonal. Setiap varietas terdiri atas umur muda (0-4 bulan), sedang (5-8 bulan) dan tua (9-12 bulan). Pengamatan kejadian dan keparahan penyakit dilakukan secara langsung di lapang. Serangga hama dan bagian tanaman terinfeksi poatogen di bawa ke laboratorium untuk diidentifikasi. Hama yang ditemukan menyerang tanaman pisang selama pengamatan yaitu Erionota thrax (L) (Lepidoptera: Hespiriidae) dan Nacoleia octasema (Meyr.) (Lepidoptera: Pyralidae). Penyakit yang ditemukan adalah bercak sigatoka yang disebabkan oleh Mycospherellla musicola dan layu fusarium yang disebabkan oleh Fusarium oxysporum f.sp. cubense. Fitonematoda yang ditemukan pada tanaman ini merupakan Radopholus sp. dan Helicotylenchus sp. Populasi dan serangan E. thrax tertinggi terdapat pada varietas Barangan pada umur sedang sedangkan N. octasema paling banyak ditemukan pada varietas Mas Kirana. Umur tanaman pisang yang semakin tua meningkatkan kejadian dan keparahan penyakit bercak sigatoka. Varietas Cavendish memiliki kejadian penyakit layu fusarium paling tinggi.
Co-Authors , Widodo . Giyanto Abdjad Asih Nawangsih ABDUL MUNIF Abdul Munif Ali Wafa Alisya Talita Papona Aloysius Rusae Ana Septiana Saputri Andika Septiana Suryaningsih Anis Mutirani Atit Kanti Atit Kanti Bonjok Istiaji Bonny Poernomo Wahyu Soekarno Bonny Poernomo Wahyu Soekarno Dede Maryana Desi Andini Desi Trisnawati Desy Nur Avifah devi ayu komalaningrat Diny Dinarti Dwi Andini Shiami Eka Wijayanti Evan P. Ramdan Fitri Kemala Sandra Fitrianingrum Kurniawati, Fitrianingrum Giyanto Giyanto Giyanto Hagia Sophia Khairani Heriyanto Syafutra Hermanu Triwidodo I Made Sudiana I Made Sudiana I Nyoman Sumerta Idris Idris Irwanto Sucipto Jean Nihana Manalu Kartini Budiastuti Kikin H Mutaqin Kustiariyah Tarman Kusumah, Yayi Munara Lilik Pujantoro Eko Nugroho Lilik Retnowati Maggy Thenawidjaja Suhartono Marianah, Lisa Masrukhin Masrukhin Meity Suradji Sinaga Meity Suradji Sinaga Meity Suradji Sinaga MEITY SURADJI SINAGA Melli Fitriani nFN Khaerati Nofiyanti, Sri Handayani Nurholis Nurholis Papona, Alisya Talita Parlindo, Fitra Paulus Woyen Pratiwi, Rizky Nomi Ramdan, Evan Purnama Rika Estria Gurusinga Rizky G S Purnama Sari, Rahmah Dian Sa’adah, Rima Nur Halimatu Siti Juariyah Slamet Widodo Slamet Widodo SRI HENDRASTUTI HIDAYAT Sukmana, Andra Sahab Supramana Surono Surono Suryo Wiyono Syam, Nur Asmasari Tanjung, Mei Rani Toga Pangihotan Napitupulu TRI ASMIRA DAMAYANTI Usman Ahmad Wawan Setiawan Widodo Widodo Widodo Widodo Widodo Widodo Yadi Suryadi Yayu Siti Nurhasanah Yunus Effendi