Claim Missing Document
Check
Articles

Found 1 Documents
Search
Journal : Pelita Perkebunan

Effectiveness of Endophytic Bacterial Consortium of Coffee Plant on Mortality of Pratylenchus Coffeae in Vitro dwi halimah; Abdul Munif; Giyanto Giyanto
Pelita Perkebunan (a Coffee and Cocoa Research Journal) Vol 31 No 3 (2015)
Publisher : Indonesian Coffee and Cocoa Research Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iccri.jur.pelitaperkebunan.v31i3.205

Abstract

Bacteria live in wild in form of a consortium. Use of microbial consortium tends to give better results than single isolate, because the action of enzyme of each type of microbe can complement each other in order to survive. This study aimed to study the effectiveness of bacterial endophytic consortium from coffee plant on plant growth and mortality of parasitic nematodes in coffee. Isolation of bacteria is conducted  by growing the crushed roots, stems and leaves of coffee on 20% TSA media, then testing their hemolysis and hypersensitivity reaction. Selected isolates were tested on their effect on the growth of seedling and Pratylenchus coffeae mortality, as well as their chitinolytic, proteolytic, lipolytic, HCN production, dissolution of phosphate (P) and fixation of nitrogen (N2) abilities. The results showed that from 27 isolates of the consortium, 23 isolates showed negative reaction to hypersensitive test and 9 isolates to hemolysis test. The highest mortality rate was shown by K6 isolate (65.8%). The highest growth was shown by K15 and K 21 isolates while the highest root length by K21 isolate. Further analisys showed that 100% of the isolates could hydrolyze proteases, lipid, and produce HCN, while chitinolytic activity was shown by 78% isolates which could fix N2 and 11% of isolates could dissolve phosphate.
Co-Authors Abdjad Asih Nawangsih Ade Maulana Agus PURWANTARA Akhmad Rizali ALI NURMANSYAH Alina Akhdiya Amanda, Dila Andika Akbarul Iman Andini Hanif Ankardiansyah Pandu Pradana Ankardiansyah Pandu Pradana Arif Ravi Wibowo Arifda Ayu Swastini Waruwu Asima Manurung Ayu Dhisa Faradiba Siregar Bonny Poernomo Wahyu Soekarno Bonny PW Soekarno Chandra, Wenripin Charles Simamora Cici Indriani Dalimunthe Dede Setiadi Deden Dewantara ERIS Desmon Gunadi Siagian Diana Putri Didy Sopandie DWI ANDREAS SANTOSA dwi halimah Dwi Halimah Efi Toding Tondok Elis Nina Herliyana Elly Rosmaini Ely Lailatul Maghfiroh Ely Lailatul Maghfiroh Endah Retno Palupi Erwin Eryna Elfasari Rangkuti Fitrianingrum Kurniawati, Fitrianingrum Gede Suastika Giyanto Giyanto Giyanto Giyanto Gusmaini Gusmaini GUSMAINI GUSMAINI Hanifah Nuryani Lioe HARNI, RITA Hasudungan, Raju I Wayan Winasa IBNUL QAYIM IKA MUSTIKA Irwanto Sucipto ISKANDAR ZULKARNAEN SIREGAR isnainy dinul Mursyalatiyus Iswandi Anas Chaniago Ita Sulistiawati JOHANNES HALLMANN John Putra S Tampubolon Jonathan Liviera Marpaung Joni Kartika Kartika Katrin Jenny Sirait Katrin Jenny Sirait Kholil Ma’ruf Kikin H Mutaqin Kristiana kristiana Kurniawan Effendi Kurniawan Effendi Kusumah, Yayi Munara Liza Sakbani Marbun, James Piter Marihat Situmorang Marpaung, J.L. Marpaung, TJ Mira Febrianti Mochamad Yadi Nurjayadi Mochamad Yadi Nurjayadi Mohamad Rahmad Suhartanto Mohammad Yadi Nurjayadi Muhammad Firdaus Oktafiyanto MUSTIKA, IKA Nababan, Esther Sorta Mauli Nela Zahara Nela Zahara Neng Tipa Nursipa Niken Nur Kasim Nurliani Bermawie NURLIANI BERMAWIE Parapat Gultom Pradana Pandu Ankardiansyah Prayogo Probo Asmoro Rachmad Sitepu Ramdan, Evan Purnama Reni Rinika resky wulandari r jahuddin RICHARD A SIKORA RITA HARNI Rita Harni Rita Harni Rizky Mailani Rahman Robin Roedhy Poerwanto Sandra Arifin Aziz Satriyas Ilyas Siagian, Desmon Siagian, Riris Regita Cahyani Simbolon, Lolyta Damora Sirimavo, Yana Siringoringo, Yan Batara Putra Siswanto siswanto Sitanggang, Fetrisia Ayu Ashari Supramana Surono Surono Suryo Wiyono Suwarno . Syahputra, Muhammad Romi Syahputra, Razak Kurnia Syaiful Anwar Sylvia, Bebby Tatit Sastrini Togu P Marpaung Umi Hidayati Widi Amaria Widya Sari Winnugroho Wiratman, Manfaluthy Hakim, Tiara Aninditha, Aru W. Sudoyo, Joedo Prihartono Yadi Suryadi Yudhistira Adhitya Pratama Yudiyanto Yudiyanto Zahedi