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Musyirna Rahmah Nst
Sekolah Tinggi Ilmu Farmasi Riau
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Earth & Planetary Sciences Economics, Econometrics & Finance Education Electrical & Electronics Engineering Energy Engineering Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Nursing Physics Public Health Social Sciences Other

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Journal : Medical Laboratory Technology Journal

 1 
The Isolatıon, Characterızatıon, and Antıfungal Assay of Lactıc Acıd Bacterıa Isolated from Green Glutınous Rıce Tape Agaınst Aspergillus flavus Nasution, Musyirna Rahmah; Aulya, Nurliza; Dona, Rahma; Ningsih, Yozi Fiedya
Medical Laboratory Technology Journal Vol. 11 No. 1 (2025): June
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31964/mltj.v11i1.645

Abstract

Green glutinous rice tape is a fermented food made from glutinous rice, katuk leaves (Sauropus androgynus), and yeast, originating from Tembilahan, Riau Province. This product can serve as a source of lactic acid bacteria (LAB) capable of producing antimicrobial compounds to inhibit pathogenic microorganisms, including Aspergillus flavus, a well-known spoilage fungus in food products. However, to date, no reports have explored the ability of LAB from green glutinous rice tape to inhibit the growth of Aspergillus flavus. This study aimed to isolate LAB from green glutinous rice tape, conduct macroscopic, microscopic, and biochemical characterizations, and evaluate the antifungal activity of the LAB isolates against Aspergillus flavus using the good diffusion method. The isolation process yielded four LAB isolates from Tembilahan green tape (TKHT-2, TKHT-3, TKHT-5, and TKHT-7), which were identified as members of the genus Lactobacillus sp., with antifungal activities of 25.50 ± 5.78 mm, 24.33 ± 0.62 mm, 22.16 ± 6.56 mm, and 18.66 ± 4.28 mm, respectively. The corresponding cell-free supernatants (CFS) from these isolates (TKHT-S2, TKHT-S3, TKHT-S5, and TKHT-S7) also demonstrated antifungal activity with inhibition zones of 19.83 ± 3.47 mm, 19.83 ± 4.24 mm, and 14.50 ± 3.26 mm, respectively. LAB cells and their cell-free supernatants partially inhibited Aspergillus flavus, indicating a fungistatic effect. These findings suggest the potential application of LAB from green glutinous rice tape as a natural preservative or antifungal agent in food products.
Co-Authors -, Hasmalina Abdi Wira Septama Abdifi, Reza Afnita, Fakhiratunnisa Afrida Yeti Afrizal Tanjung Agfa Febriyananda Agustini, Tiara Tri Akmal Djamaan Amnan, Wekis Raya Anggraini, Annisa Fitri Anita Lukman Annisa Riski Permata Sari Antasya, Vaylia Aqilla, Alya Armon Fernando Armon Fernando Arundita, Silvy Aulya, Nurliza Ayuni, Putri Azhari, Weriska Restu Bella Ardhiyati BENNI ISKANDAR Cahyani, Ardhia Regita Dasni Syafril Dasni Syafril Deni Anggraini Diwa, Resi Oktamara Dwi Andreyas Elsa Etavianti Emrizal Enda Mora Etavianti, Elsa Febrian, Dyan Fina Aryani, Fina Firman Nugroho Fitri, Ramadini Fitri, Valentina Furi, Mustika Ginta Rio Maranti Gressy Novita Gustina ' Haiyul Fadhli Hanifa, Elvi Hasmalina - Hasmalina Nasution Husnawati Ihsan Ikhtiarudin Intan Putri Utami Irwan Irwan Jannah, Ninuk Rodhiatul Ladiona, Maria Yella Lamun Bathara Manik, Dhea Putri Bertuah Br Maria Yella Ladiona Martina Br Manullang Masnun, Lidiatil Meiriza Djohari, Meiriza Melati, Putri Melzi Octaviani Milianty, Jesy Muthmainnah, Nurillah Nabilah, Clara Nabilla, Vivi Gita Nanang Nasution, Tio Afandi Neni Frimayanti Ningrum, Tilar Eka Widia Ningsih, Yozi Fiedya Nur Rahmadani Fitri Nurhapipah, Nurhapipah Pardede, Depa Gloria Kristin Peng, Wei-Ching Peng-Wei, Ching Putri Lestari, Putri Putri Putri, Putri Rabiatul Adawiyah Rahayu Utami Rahayu Utami Utami Rahma Dona Rahman, Sumiati Rajeb Fadillah ramadhan, alfitrah Ramadhani, Elsha Fadhika Dwi Ratna Sari Dewi Reza Abdifi Ronapadua Musyirna Rahmah Nasution Sahara dan Emma Susanti Ronapadua Sahara Ruska, Shinta Liana S.Farm., M.Farm., Apt, Sondang Khairani Safitri, Ramanda Sahara, Ronapadua Sakinah, Norma Septi Muharni Silvera Devi Simanjuntak, Asnika Putri Sri Hardianti Sudarsi, Yulisma Suhelmi - Suhelmi - Sumiati Rahman Syahbandi, Ilham Syahril Nedi Syamira Tri Budhi Murdiati Tri Budhi Murdiati Tria Halianti Triani, Leni Winda Sri Wahyuni Wira Noviana, Wira Noviana Wulan Desmar Utari Yuharmen - Yulisma Sudarsi Yunelva, Rinda