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Profil Antioksidan Minuman Sari Tempe Berbahan Dasar Tepung Tempe Original dan Tepung Tempe Kelor Andiniyati, Fitri; Bintari, Siti Harnina; Dewi, Pramesti; Mustikaningtyas, Dewi
Life Science Vol 12 No 1 (2023): May 2023
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/lifesci.v12i1.66771

Abstract

Tepung tempe dapat dijadikan sebagai substitusi makanan atau suplemen antara lain minuman sari tempe. Namun, minuman sari tempe saat ini belum banyak diproduksi. Padahal minuman sari tempe memiliki efek fungsional untuk menjaga kesehatan tubuh. Perlu adanya pengujian terhadap kualitas minuman sari tempe meliputi nilai aktivitas antioksidan, kadar protein, dan tingkat kesukaan terhadap produk minuman sari tempe berbahan dasar tepung tempe original dan tepung tempe kelor dengan konsentrasi yang berbeda yaitu 1%, 2%, dan 3%. Tujuan penelitian ini adalah mengetahui nilai aktivitas antioksidan dan kadar protein minuman sari tempe berbahan dasar tepung tempe original dan tepung tempe kelor dengan konsentrasi 1%, 2%, dan 3%, dan mengetahui minuman sari tempe yang paling disukai konsumen. Penelitian ini merupakan penelitian eksperimen Rancangan Acak Lengkap (RAL) dengan enam perlakuan berupa minuman sari tempe berbahan dasar tepung tempe original konsentrasi 1%, minuman sari tempe berbahan dasar tepung tempe original konsentrasi 2%, minuman sari tempe berbahan dasar tepung tempe original konsentrasi 3%, minuman sari tempe berbahan dasar tepung tempe kelor konsentrasi 1%, minuman sari tempe berbahan dasar tepung tempe kelor konsentrasi 2%, dan minuman sari tempe berbahan dasar tepung tempe kelor konsentrasi 3%. Masing-masing perlakuan dilakukan dua kali ulangan. Pengujian aktivitas antioksidan menggunakan metode DPPH, pengujian kadar protein menggunakan metode Kjeldhal, dan pengujian tingkat kesukaan menggunakan metode hedonik. Data kualitatif dianalisis secara deskriptif, sedangkan data kuantitatif dianalisis menggunakan uji Anova dua arah dan uji lanjut Duncan. Hasil penelitian menunjukkan minuman sari tempe yang mempunyai nilai aktivitas antioksidan sangat kuat adalah minuman sari tempe berbahan dasar tepung tempe kelor dengan konsentrasi 1% yang ditunjukkan dengan nilai (IC₅₀ < 50), minuman sari tempe dengan kadar protein tertinggi ada pada minuman sari tempe berbahan dasar tepung tempe kelor dengan konsentrasi 3%, dan minuman sari tempe yang paling disukai oleh konsumen adalah minuman sari tempe berbahan dasar tepung tempe original dengan konsentrasi 1%.
Pengaruh Variasi Metode Ekstraksi Metabolit Sekunder Bakteri Endofit Daun Rambutan (Nephelium lappaceum L.) terhadap Pertumbuhan Escherichia coli dan Bacillus subtilis Hasyim Abdurrasyid; Ibnul Mubarok; Dewi Mustikaningtyas; Pramesti Dewi
Life Science Vol. 13 No. 2 (2024): November 2024
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/unnesjlifesci.v13.i2.2327

Abstract

Rambutan (Nephelium lappaceum L.) is one of the many tropical plants found in Indonesia. The leaves of the rambutan plant are known to be used as medicine, so they have the potential to be a source of endophytic bacteria that can produce beneficial compound. This study aims to isolate, identify of endophytic bacteria from rambutan leaves, and test their activity as an antibacterial with two different secondary metabolite extraction methods, namely maceration with 70% methanol and without maceration. This study used experimental research with a Completely Ramdomized Design (CRD). Isolation of endophytic bacteria from rambutan leaves produced two isolates (D1 and D2). The results of identification through macroscopic, microscopic, and biochemical test showed that two pure isolates had similarities to genus Bacillus sp. Antibacterial activity testing was carried out using well diffusion method. Based on the results of antibacterial test showed that extraction method with 70% methanol produced an extract that was more effective as an antibacterial with average diameter of clear zone formed for Escherichia coli bacteria were 13.55 mm (Isolate D1) and 12.13 mm (Isolate D2) and that of Bacillus subtilis bacteria were 16.27 mm (Isolate D1) and 19.69 mm (Isolate D2). This finding contributes to the fact that the type of secondary metabolite extraction method of rambutan leaf endophytic bacteria influences the sensivity as an antibacterial.
Pengaruh Lama Penyimpanan Biji Kopi Liberika pada Suhu Kamar terhadap Mutu Mikrobiologis dan Kadar Okratoksin A: The Effect of Storage Time of Liberica Coffee Beans at Room Temperature on Microbiological Quality and Ochratoxin A Levels Ramadani, Mutiara Bintang; Dewi Mustikaningtyas; Siti Harnina Bintari; Pramesti Dewi
Life Science Vol. 14 No. 1 (2025): May 2025
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/unnesjlifesci.v14.i1.5810

Abstract

Liberica coffee is one of the leading commodities in several regions so it has quite high economic potential. Coffee beans can be stored for quite a long time, more than one year, even more than 4 years before going through the roasting process. However, during long storage periods coffee beans can be attacked by microorganisms such as mold (ochratoxigenic and non-ochratoxigenic). This research aims to determine the effect of storage time on the quality of Liberica coffee beans, both microbiologically and chemically. The benefit of this research is to provide information on the quality of Liberica coffee beans. This research is a laboratory experimental research. The long storage time for coffee beans was 36 and 39 weeks. The results of the research showed that the yeast mold rate (AKK) on Liberica coffee beans stored for 39 weeks was greater than those stored for 36 weeks. The increase in AKK during storage is thought to be due to the presence of insects, temperature and humidity factors. A total of three mold isolates were found at week 36 and there were six mold isolates at week 39. The percentage of fungal isolate attacks at both weeks 36 and 39 was dominated by fungi of the Mucorales order (Mucor sp. and Absidia sp.). In the 36th week the attack of Mucor sp. amounted to 36.67% while in the 39th week the Mucor sp attack. 20%. and Absidia sp. 23.33%. Ochratoxin A (OTA) levels from week 36 to week 39 did not increase but instead decreased. This occurs due to OTA degradation by ochratoxin-degrading molds which are thought to be Aspergillus sp. during storage.
Antibacterial Activity Test of the Combination of Bay Leaf (Syzygium polyanthum) Extract and Nanochitosan Against Escherichia coli and Bacillus subtilis Astrid Farmayati; Siti Harnina Bintari; Pramesti Dewi; Dewi Mustikaningtyas
Life Science Vol. 14 No. 1 (2025): May 2025
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/unnesjlifesci.v14.i1.24777

Abstract

Indonesia is one of the world’s most biodiverse countries, including in medicinal plants that contain active compounds with potential as sources of modern medicine, particularly for treating bacterial infections. The use of herbal plants as antibacterial agents offers an alternative to overcome antibiotic resistance and the side effects of synthetic drugs. Bay leaf (Syzygium polyanthum) and nanochitosan are known for their antibacterial properties due to their bioactive compounds. However, studies on their combined effects are still limited. This study aimed to evaluate the antibacterial effectiveness of combinations of bay leaf extract (15%, 25%, and 35%) and nanochitosan (0.5% and 1%) against Escherichia coli and Bacillus subtilis. A Completely Randomized Design (CRD) was applied, using sterile distilled water as a negative control and 0.1% chloramphenicol as a positive control. The antibacterial activity test was performed using the Kirby-Bauer disk diffusion method. Results showed the positive control produced inhibition zones of 25.45 ± 1.27 mm for E. coli and 25.05 ± 0.39 mm for B. subtilis. The most effective combination was K3 (35% bay leaf extract and 0.5% nanochitosan), which produced inhibition zones of 12.65 ± 0.52 mm and 12.91 ± 0.55 mm, respectively. Statistical analysis using the Tukey HSD post hoc test confirmed significant differences among treatments. These findings suggest that the combination of bay leaf extract and nanochitosan holds promise as an alternative antibacterial agent
Computer Assisted Instruction Media: Impact to Students Digital Literacy and Learning Outcomes Gunawan, Arvidhea Safira; Dewi, Pramesti; Mustikaningtyas, Dewi
Unnes Science Education Journal Vol. 13 No. 1 (2024): April 2024
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/usej.v13i1.1530

Abstract

This study aims to determine the impact of utilization Computer Assisted Instruction (CAI) based learning media in improving digital literacy skills and student learning outcomes. In this study, data collection used observation sheets to improve students' digital literacy skills through observations of experimental and control groupes. It is known that the score of students' digital literacy skills is 81% in the experimental group, while the control group is 67%, meaning that the criteria for students' digital literacy skills in the experimental group are classified as better.  In collecting data to determine the effect of media on student learning outcomes, the Pretest Posttest control group design method is used with a pretest, treatment, and posttest pattern. In practice, 2 groupes are needed as experimental groupes with treatment and control groupes without treatment, and observations are made of students' digital literacy skills during learning activities. From the results of the analysis of student pretests and posttests, it is known that there is an increase in scores in the experimental group as indicated by the average N-Gain value of 57.54%, thus the use of CAI-based learning media is interpreted in the category quite effective in improving student learning outcomes. Then based on the results of the one-way anova analysis for the relationship between the improvement of learning outcomes with digital literacy skills in experimental and control groupes, it is known that the significance value is 0.00 <0.05, as well as the results of the post-hoc test with the Turkey method which shows the significance value between digital literacy and learning outcomes is less than 0.05, which means that the use of CAI-based learning media has an influence on digital literacy as well as student learning outcomes. So it can be concluded that the use of CAI-based media has an impact in improving digital literacy skills and student learning outcomes.
Faktor-Faktor Yang Berhubungan Dengan Kejadian Ponv Pada Pasien Pasca Anestesi Umum Di Rumah Sakit Islam Banjarnegara Lestishiyami, Lesy; Suryani, Roro Lintang; Dewi, Pramesti; Suandika, Made
Jurnal Ilmiah Wahana Pendidikan Vol 10 No 7 (2024): Jurnal Ilmiah Wahana Pendidikan
Publisher : Peneliti.net

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5281/zenodo.11160778

Abstract

Latar Belakang : Setelah pembedahan, efek samping yang paling umum setelah anestesi umum adalah muntah pasca operasi (PONV). Efek samping ini termasuk sakit tenggorokan, delerium, nyeri otot, gatal, hipotermia, dan PONV. Tujuan : untuk mengetahui penyebab PONV pada pasien pasca anestesi umum di Rumah Sakit Islam Banjarnegara. Metode : Studi analitik deskriptif ini menggunakan metode cross-sectional. Sampel penelitian terdiri dari 80 orang yang mengikuti penelitian. Hasil : Studi ini menemukan bahwa pada pasien pasca anestesi umum di Rumah Sakit Islam Barnjarnegara, usia, jenis kelamin, durasi operasi, dan status fisik ASA memiliki hubungan yang signifikan dengan PONV. Dengan nilai signifikasi di bawah 5% (<0.05), data chi-square menunjukkan ini. Simpulan : korelasi yang signifikan antara usia pasien (p=0,040), jenis kelamin (p=0,011), dan durasi operasi (p=0,014) dengan kejadian PONV pada pasien pasca operasi di Rumah Sakit Islam Banjarnegara.
Pengaruh Ekstrak Buah Pinang Muda (Areca catechu) Terhadap Pertumbuhan Kapang Pada Rimpang Jahe Gunawan, Arvidhea Safira; Mustikaningtyas, Dewi; Dewi, Pramesti; Widiatningrum, Talitha
Bioeksperimen: Jurnal Penelitian Biologi Vol 9, No 1: March 2023
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v9i1.17203

Abstract

Jahe merupakan tanaman herbal dengan peminat yang tersebar di seluruh dunia. Menurut data yang disebutkan oleh Food and Agriculture Organization di tahun 2019, produksi jahe di Indonesia sebanyak 174 ribu ton dan Indonesia menempati urutan keenam sebagai negara dengan hasil produksi jahe terbesar di Asia. Tingginya minat masyarakat terhadap jahe mengakibatkan melonjaknya produksi jahe sehingga muncul permasalahan baru salah satunya kerusakan pada rimpang jahe. Sehingga alternatif pengendalian dengan fungisida alami diperlukan. Buah pinang memiliki kandungan senyawa bioaktif seperti saponin, flavonoid, tannin, phenol, steroid, alkaloid yang dapat berperan sebagai anti-kapang. Penelitian ini bertujuan untuk mengetahui pengaruh penambahan ekstrak buah pinang muda terhadap  pertumbuhan kapang pada rimpang jahe segar dan simplisia kering rimpang jahe. Hasil yang diperoleh pada penelitian ini adalah adanya penghambatan pertumbuhan kapang dari penambahan ekstrak dengan pengenceran. Sedangkan perlakuan ekstrak tanpa pengenceran kurang memberikan pengaruh dalam penghambatan pertumbuhan kapang.
Comparison of the Rate and Level of Biodegradation of Leaf Waste by Lignocellulolytic Molds from Universitas Negeri Semarang Campus Walandipa, Ghani; Mustikaningtyas, Dewi; Widiatningrum, Talitha; Dewi, Pramesti
Life Science Vol. 14 No. 2 (2025): November 2025
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/unnesjlifesci.v14.i2.25828

Abstract

Biodegradation is a physical decomposition of the substrate caused by the activity of microorganisms by producing products that have benefits for humans. Lignocellulose degrading fungi are microorganisms that play a role in the process of decomposing organic waste. The biodegradation rate of each type of lignocellulose degrading mold is different. The purpose of this research is to comprehensively analyze and compare the rate and level of biodegradation of leaf waste in lignocellulolytic molds isolated from the campus environment of Universitas Negeri Semarang, as well as to identify and evaluate the type of lignocellulolytic mold that shows the highest efficiency in the rate and level of biodegradation of leaf waste. The method used in this research is quantitative method, by measuring the mass ratio and pH of leaf waste media before inoculation and after inoculation. The results of this study are the rate of leaf waste biodegradation based on the ratio of leaf waste mass by Trichoderma koningiopsis mold isolate of 0.015 grams/day, while by Trichoderma erinaceum mold isolate of 0.002 grams/day. Then the rate of leaf waste biodegradation based on the pH change value of leaf waste by Trichoderma koningiopsis mold isolate is higher with a pH change value of 0.3 when compared to the Trichoderma erinaceum mold isolate with a pH change value of 0.1. The superior performance of Trichoderma koningiopsis highlights its potential application in optimizing organic waste decomposition systems and provides a scientific basis for selecting lignocellulolytic fungi in sustainable waste management programs.
Hubungan Tingkat Pengetahuan Dengan Keterampilan Kader Tentang Manajemen Nyeri Non Farmakologis Bagi Pasien Lansia Pada Kader Di Kelurahan Sumampir Wirakhmi, Ikit Netra; Ningrum, Ema Wahyu; Dewi, Pramesti
Jurnal Ilmiah Kesehatan Keperawatan Vol 21, No 2 (2025): JURNAL ILMIAH KESEHATAN KEPERAWATAN
Publisher : LPPM UNIVERSITAS MUHAMMADIYAH GOMBONG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26753/jikk.v21i2.1942

Abstract

Kader kesehatan memiliki peran penting dalam upaya deteksi dini dan pencegahan penyakit di masyarakat. Pengetahuan dan keterampilan kader menjadi faktor utama yang menentukan efektivitas pelaksanaan tugas mereka. Penelitian ini bertujuan untuk menganalisis hubungan antara pengetahuan dan keterampilan kader dalam pelaksanaan kegiatan kesehatan masyarakat. Desain penelitian yang digunakan adalah deskriptif korelasional dengan pendekatan cross sectional. Pengambilan sampel dilakukan secara purposive terhadap kader aktif di wilayah kerja tertentu. Data dikumpulkan melalui kuesioner pengetahuan dan lembar observasi keterampilan, kemudian dianalisis menggunakan uji statistik korelasi Spearman. Hasil penelitian menunjukkan bahwa sebagian besar kader berada pada kategori usia dewasa (19–59 tahun) sebesar 87,8%. Ditemukan adanya hubungan signifikan antara pengetahuan dan keterampilan kader (p = 0,01) dengan tingkat keeratan hubungan sangat lemah (r = 0,275). Temuan ini menunjukkan bahwa peningkatan pengetahuan dapat berkontribusi terhadap peningkatan keterampilan kader, meskipun dengan pengaruh yang terbatas. Dapat disimpulkan bahwa pengetahuan merupakan salah satu faktor yang berperan dalam meningkatkan keterampilan kader, namun perlu didukung dengan pelatihan berkelanjutan, motivasi, dan dukungan tenaga kesehatan agar kompetensi kader dapat berkembang secara optimal. Health cadres play an essential role in early disease detection and prevention within the community. Knowledge and skills are key factors determining the effectiveness of cadre performance in implementing health programs. This study aimed to analyze the relationship between knowledge and skills among health cadres in community health activities. A descriptive correlational design with a cross-sectional approach was employed. Samples were selected purposively from active cadres within a designated working area. Data were collected using a knowledge questionnaire and a skill observation sheet, then analyzed using the Spearman correlation test. The results showed that most cadres were in the adult age category (19–59 years), accounting for 87.8% of participants. There was a significant relationship between knowledge and skills (p = 0.01) with a very weak correlation (r = 0.275). These findings indicate that increased knowledge contributes to improved cadre skills, although the influence is limited. It can be concluded that knowledge is one of the contributing factors in enhancing cadre skills; however, continuous training, motivation, and support from health professionals are necessary to optimize cadre competence in community health practice.
Pemberdayaan Ibu-Ibu PKK dalam Pembuatan Pupuk Organik untuk Mendukung Urban Farming di Kelurahan Kalirejo, Kecamatan Gunungpati, Kota Semarang Lina Herlina; Pramesti Dewi; Wulan Christijanti; Talitha Widiatningrum
ABDIMAS Jurnal Pengabdian Kepada Masyarakat Vol. 6 No. 2 (2025): ABDIMAS JURNAL PENGABDIAN KEPADA MASYARAKAT
Publisher : Research and Community Service INSTITUT TEKNOLOGI DAN BISNIS KALBIS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53008/qe2bsj27

Abstract

Program pengabdian kepada masyarakat ini bertujuan untuk memberdayakan ibu-ibu anggota PKK di RT 03/RW 05, pada pembuatan pupuk organik dari limbah rumah tangga. Pelatihan ini dilakukan guna mendorong peningkatan pemahaman serta keterampilan ibu-ibu PKK dalam pembuatan pupuk organik berkualitas secara mandiri. Selain itu program ini bertujuan  dalam mendorong praktik urban farming di lingkungan perkotaan, yang berkontribusi pada ketahanan pangan lokal, pengelolaan sampah organik yang lebih baik, dan menciptakan lingkungan yang lebih hijau dan sehat. Metode yang dipergunakan pada program ini mencakup sosialisasi, pelatihan pembuatan pupuk organic, serta pendampingan berkelanjutan. Hasil dari program ini mengindikasikan peningkatan pengetahuan serta keterampilan ibu-ibu PKK terkait pembuatan pupuk organik. Selain itu, inisiatif urban farming di lingkungan tempat tinggal peserta juga mengalami peningkatan, ditandai dengan pemanfaatan pupuk organik hasil pelatihan pada tanaman yang mereka budidayakan. Kegiatan ini menunjukkan  bahwa pemberdayaan masyarakat melalui pelatihan keterampilan praktis dapat menjadi solusi efektif dalam mendukung pertanian perkotaan yang berkelanjutan.
Co-Authors Adiratna Sekar Siwi Adystia, Yusdike Ahmad Agus Prasetyo Ananda, Rahimma Artantia Andiniyati, Fitri Ari Yuniastuti Arvidhea Safira Gunawan Astrid Farmayati Atun Raudotul Ma&#039;rifah Atun Raudotul Ma’rifah Atun Raudotul Ma’rifah Cahyaningrum, Etika Dewi Danang Dwi Saputro Devi Nisa Hidayati Dewi Mustikaningtyas Dewi Mustikanintyas Dhimas Fajar Eka Purnama Dyah Rini Indriyanti Elawati, Nunung Eni Enni Suwarsi Rahayu Esti Isussilaning Setiawati Fadhiatul Mufrihah Fajarsari, Nurida A’sha Farreza, Delia Alifia Fauziah Hanum Nur Adriyani Felintina Yuniarti, Felintina Ghani, Farid Gunawan, Arvidhea Safira Hartini, Tuti Hasyim Abdurrasyid Hidayatullah, Riki Hilmi, Aanisah Ibnul Mubarok Ibnul Mubarok Ibnul Mubarok Indrati, Retno Indri Heri Susanti Isnaeni Karunia Annisa Jelita, Orchida Puspa Khairul Anam Kirana, Swastika Nur La’ali, Shaimaa Alma Lestishiyami, Lesy Lina Herlina Lintang Suryani, Roro Lisdiana Lisdiana Made Suandika Maria Ayu Puspita Mariah Ulfah Martien Herna Susanti Maya Safitri Maya Safitri meidina rahmawati Melinda, Aura Tirsa Murniati Nindia Sefi Utami Ning Setiati Ningrum, Ema Wahyu Noor Rochmah Ida Ayu Trisno Putri Noor Yunida Triana Nur Rohmah Tria Romadhoni Opty Prastika, Luvi Prapto Sasongko, Didik Pratama, Fisky Aditya Priyantini Widiyaningrum Purwono Purwono, Purwono Puspita Diana Arumawati rahmawati, meidina Rahmaya Nova Handayani Rakhmasari, Pramesti Regita Ramadani, Mutiara Bintang Reni Dwi Setyaningsih Retno Indrati Retno Sri Iswari Ria Millati Ria Millati Rusuli, Alvin Maulana Sadikin, Nadya Audina Nurkhafiya Safar Dwi Kurniawan Sardjono Sardjono Sardjono Sardjono Sekar Dwi Ardianti Sigit Saptono Silvia Nurbaiti, Silvia Siswahyudianto Siti Haniyah Siti Harnina Bintari Siti Wahidatun Nisa Soniawati, Diah Steffani Fransisca Suci Khasanah Sugiarta, Nyoman Sulasfiana Alfi Raida, Sulasfiana Alfi Sunyoto Sunyoto SURTININGSIH, SURTININGSIH Suryani, Roro Lintang Susanto, Amin Syamsul Aryanto Talitha Widiatningrum Tin Utami Tri Retno Nurasih Tri Sumarni Tri Yudono, Danang Triana, Noor Yunida Tyas Agung Pribadi, Tyas Agung Walandipa, Ghani Wibowo, Tophan Heri Wirakhmi, Ikit Netra Woung-Ru Tang Wulan Christijanti Yance Anas Yati, Ernallah Yusdike Adystia Yustinus Ulung Anggraito Zahwa, Iana