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All Journal Jurnal Sains Dasar Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Biology, Medicine, & Natural Product Chemistry International Journal of Applied Biology Health Notions JRST (Jurnal Riset Sains dan Teknologi) Journal of Islamic Medicine Jurnal Biolokus: Jurnal Penelitian Pendidikan Biologi dan Biologi Journal of Health Science and Prevention Best Journal (Biology Education, Sains and Technology) Journal of Innovation in Educational and Cultural Research Indian Journal of Forensic Medicine & Toxicology Jurnal Jeumpa STRATEGY : Jurnal Inovasi Strategi dan Model Pembelajaran BIOMA : Jurnal Biologi dan Pembelajarannya Jurnal Sosial dan Sains Zona Education Indonesia Indonesian Journal of Biology Education BIOTROPIC
Risa Purnamasari
Universitas Islam Negeri Sunan Ampel Surabaya
Religion Agriculture, Biological Sciences & Forestry Humanities Astronomy Automotive Engineering Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Dentistry Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Library & Information Science Medicine & Pharmacology Nursing Physics Public Health Social Sciences Other

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Kemampuan Bacillus subtilis dalam mendegradasi limbah plastik polystyrene ditinjau dari perubahan berat kering dan spektrum Fourier Transform Infra-Red (FTIR) Putri Darmansyah, Alifia; Purnamasari, Risa; Wazna Auvaria, Shinfi
Biotropic : The Journal of Tropical Biology Vol. 9 No. 2 (2025): Biotropic, Volume 9 Nomor 2, 2025
Publisher : Program Studi Biologi, Fakultas Sains dan Teknologi, Universitas Islam Negeri Sunan Ampel Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29080/biotropic.v9i2.2373

Abstract

Polystyrene is a type of plastic that is difficult to decompose naturally and  one of the main causes of environmental problems, especially in Indonesia, styrofoam waste in 2023 reached more than 32 million tons per year. This study uses quantitative descriptive and experimental methods to determine the ability of Bacillus subtilis bacteria to decompose polystyrene waste. The quantitative descriptive method was carried out by measuring the decrease in dry weight of polystyrene, while the experimental method was carried out by incubating 1x1 cm and 2x2 cm polystyrene samples with B. subtilis in Bushnell Haas media at 37°C for 40 days, with observations performed every 10 days. In addition, chemical changes in polystyrene were observed using Fourier Transform Infrared (FTIR) to see changes in functional groups. The results showed that the 1×1 cm sample experienced a weight loss of up to 20.65% after 40 days, while the 2×2 cm sample only decreased by 8.70%. FTIR analysis showed changes in absorption intensity and the appearance of carbonyl (C=O) and hydroxyl (O–H) groups, indicating the occurrence of oxidation and polymer chain cleavage. In conclusion, Bacillus subtilis was able to degrade polystyrene gradually, with effectiveness influenced by the size of the sample surface and the length of incubation time.
Effect of Different Concentration of SCOBY Kombucha as an Anti-acne Against Staphylococcus aureus in The Ear of Mice (Mus musculus) Anggun Putri Ferdyanti; Eva Agustina; Hanik Faizah; Risa Purnamasari
Biology, Medicine, & Natural Product Chemistry Vol 14, No 2 (2025)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2025.142.1143-1150

Abstract

SCOBY (Symbiotic Culture of Bacteria and Yeast) results from symbiosis between yeast and bacteria during fermentation. SCOBY kombucha gel combined with orange peel extract has antibacterial compounds that can inhibit the growth of S. aureus bacteria. The method in this study was to make variations in the concentration of SCOBY in the gel and inject S. aureus bacteria into the ears of mice. After 2x24 hours, the diameter of the inflammation caused was observed and SCOBY kombucha gel was applied (0%, 2%, 4%, 8%, 10%, and 12%), positive control (Mediclin 1%), and negative control (without gel application). The results showed that the higher the concentration of SCOBY, the faster the healing activity of the gel against S. aureus. SCOBY kombucha gel has the fastest healing activity in inhibiting acne pathogen S. aureus 12% SCOBY gel. Inflammation in the ears of mice treated with 12% SCOBY gel had healed by the 90th hour, while in other groups of mice it had only healed at the last observation hour, namely the 114th hour.
Antioxidant Activity Test of Vanilla Leaf Kombucha Tea with Variations in Fermentation Time Eva Agustina; Esti Tyastirin; Risa Purnamasari; Nova Lusiana; Funsu Andiarna
Jurnal Sains Dasar Vol. 14 No. 2 (2025): October 2025
Publisher : Faculty of Mathematics and Natural Science, Universitas Negeri Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21831/jsd.v14i2.88114

Abstract

Kombucha tea is a fermented tea leaf drink with added sugar and Symbiotic Culture of Bacteria and Yeast (SCOBY). Vanilla leaves (Vanilla planifolia) have the potential to be the basic ingredient for making kombucha tea. Variations in fermentation time in tea making can affect the content of active compounds. The purpose of this study was to determine the levels of phenolic compounds and antioxidant activity of kombucha tea with variations in fermentation time. The fermentation time for making vanilla leaf kombucha tea is 0, 4, 8, and 12 days. The phenolic compounds were known by the Folin–Ciocalteu method using the gallic acid standard, while the antioxidant activity was known by the free radical inhibition method 2,2-diphenyl-1-pycrylhydrazyl (DPPH). The results showed that the levels of phenolic compounds at fermentation times of 0, 4, 8, and 12 days were 37.99, 42.188, 57.58, respectively, and 50.53 mg/L GAE. The highest phenolic levels are found at the 8th day of fermentation. The IC50 values for fermentation times of 0, 4, 8, and 12 consecutive days were 18%, 7.9%, 4%, and 7.7% v/v. An IC50 value < 50 indicates strong antioxidant activity. Vanilla leaf kombucha tea has the potential to be a beverage product that is beneficial for health due to its content of phenolic compounds and antioxidant activity.
Potential of Cellulose from Sugarcane Bagasse as a Bioplastic Material for Drug Capsules Agustina, Eva; Erfansyah, Nasrul Fuad; Lusiana, Nova; Purnamasari, Risa
Journal of Health Science and Prevention Vol. 10 No. 1 (2026): JHSP Vol 10 No 1 – 2026
Publisher : State Islamic University of Sunan Ampel

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29080/jhsp.v10i1.1831

Abstract

Sugarcane bagasse is a lignocellulosic waste that has significant potential as a cellulose source for the development of environmentally friendly bioplastics. This study aims to This study aimed to evaluate the potential of cellulose derived from sugarcane bagasse as a bioplastic material for drug capsule applications.. The research method includes the isolation of sugarcane bagasse into cellulose and the production of bioplastics. The bioplastics were prepared in two formulations, namely P1 (without cellulose) and P2 (with the addition of sugarcane bagasse cellulose), using chitosan as the polymer matrix and glycerol as a plasticizer. Characterization was conducted through morphological analysis, mechanical properties (tensile strength and elongation), density, thickness, water absorption, and biodegradability tests. Statistical analysis using an independent samples t-test revealed significant differences between formulations. The tensile strength of P2 (39.2 MPa) was significantly higher than P1 (21.5 MPa) (t = 13.7, p < 0.001), while elongation significantly decreased from 27.58% in P1 to 17.32% in P2 (t = 10.9, p < 0.001). Density and thickness also increased significantly in P2 (0.8444 g/cm³ and 0.276 mm) compared to P1 (0.5396 g/cm³ and 0.118 mm) (t = 10.5 and 18.3; p < 0.001), indicating a more compact film structure. In contrast, water absorption showed no significant difference between treatments (t = 0.10, p = 0.92), and both samples exhibited similar biodegradation times of approximately four days (t = 0.00, p = 1.00). These result demonstrate that cellulose incorporation improves mechanical strength and structural compactness without reducing biodegradability. Cellulose-based bioplastic derived from sugarcane bagasse has the potential to be developed as an alternative soft capsule material in environmentally friendly drug delivery systems, serving as a substitute for gelatin-based capsules in the pharmaceutical industry.
Co-Authors Agustin, Azlinda Mitha Agustina, Eva AGUSTINA, EVA Aliyyah Nafiisah ANDYARINI, ESTI NOVI Anggun Putri Ferdyanti Auvaria, Shinfi Wazna Azlinda Mitha Agustin Dedy Suprayogi Esti Tyastirin Estri Kusumawati Eva Agustina Eva Agustina Eva Agustina, Eva Evi Fatimatur Rusydiyah Faidah, Nurul Ilmi Fiddaroini, Achmad Farchan Funsu Andiarna Hanik Faizah Heny Utami Ningsih Hernik Farisia Husniyatus Salamah Zainiyati Huwaidah, Jihan Farhah Ika Mustika Irul Hidayati Karim, Nuzulia Linda Prasetyaning Linda Prasetyaning W Linda Prasetyaning Widayanti Ma'arif, Muhammad Mei Lina Fitri Kumalasari Mei Lina Fitri Kumalasari Misbakhul Munir Moch. Irfan Hadi Mudalianah, Siti Muhammad Falikhul M Nabila, Salsa Nafiisah, Aliyyah Nafisah, Raihana Frika Nasrul Fuad Erfansyah Nova Lusiana Nurul Ilmi Faidah P, Ria Indria Putri Darmansyah, Alifia Qurrotul Ayun Rahayu, Lusy Ria Qadariah Arief Rony Irawanto Rony Irawanto Rony Irawanto Salsa Nabila Salsa Nabila Sarita Oktorina Shofiyya, Ainina Neihayatus Siti Mudalianah Sri Hidayati Sri Hidayati L. Sri Mulyati Supardi Tania, Clarista Eka Ulfa Wafirotul Bahiyyah