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Analisis Kuantitatif Kandungan Antioksidan dan Aktivitas Sitotoksik dari Ekstrak Anastatica hierochuntica L Nova Lusiana; Risa Purnamasari; Eva Agustina; Nurul Ilmi Faidah; Azlinda Mitha Agustin; Muhammad Falikhul M
Biotropic : The Journal of Tropical Biology Vol. 6 No. 2 (2022): Biotropic, Volume 6 Nomor 2, 2022
Publisher : Program Studi Biologi, Fakultas Sains dan Teknologi, Universitas Islam Negeri Sunan Ampel Surabaya

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Abstract

Antioxidants are compounds that can counteract free radicals in the body due to the presence of active compounds such as flavonoids and phenolics. These active compounds can be cytotoxic, ie, compounds capable of inhibiting and stopping the growth of cancer cells by using toxicity test by BSLT (Brine Shrimp Lethality Test) method. This study aims to determine the antioxidant activity by DPPH (Dipheny Pycryl Hydrazil) method and toxicity test by BSLT method of Anastatica hierochuntica L extract. A. hierochuntica L extraction were done by maceration method with methanol solvent. The content of A. hierochuntica L extract compounds was done by phytochemical test to know flavonoid compounds, triterpenoid and sterol. Meanwhile, alkaloid and saponin and liquid chromatography to see flavonoid compounds of quersetin. Furthermore, the antioxidant activity test was carry out by the DPPH method and by BSLT method to observed the mortality of shrimp larvae. A. hierochuntica extract has antioxidant activity with IC50 value 77,23 μg / ml and toxicity value of LC50 44,97. A.hierochuntica L extracts potentially have antioxidant and cytotoxic properties.
Kemampuan Bacillus subtilis dalam mendegradasi limbah plastik polystyrene ditinjau dari perubahan berat kering dan spektrum Fourier Transform Infra-Red (FTIR) Putri Darmansyah, Alifia; Purnamasari, Risa; Wazna Auvaria, Shinfi
Biotropic : The Journal of Tropical Biology Vol. 9 No. 2 (2025): Biotropic, Volume 9 Nomor 2, 2025
Publisher : Program Studi Biologi, Fakultas Sains dan Teknologi, Universitas Islam Negeri Sunan Ampel Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29080/biotropic.v9i2.2373

Abstract

Polystyrene is a type of plastic that is difficult to decompose naturally and  one of the main causes of environmental problems, especially in Indonesia, styrofoam waste in 2023 reached more than 32 million tons per year. This study uses quantitative descriptive and experimental methods to determine the ability of Bacillus subtilis bacteria to decompose polystyrene waste. The quantitative descriptive method was carried out by measuring the decrease in dry weight of polystyrene, while the experimental method was carried out by incubating 1x1 cm and 2x2 cm polystyrene samples with B. subtilis in Bushnell Haas media at 37°C for 40 days, with observations performed every 10 days. In addition, chemical changes in polystyrene were observed using Fourier Transform Infrared (FTIR) to see changes in functional groups. The results showed that the 1×1 cm sample experienced a weight loss of up to 20.65% after 40 days, while the 2×2 cm sample only decreased by 8.70%. FTIR analysis showed changes in absorption intensity and the appearance of carbonyl (C=O) and hydroxyl (O–H) groups, indicating the occurrence of oxidation and polymer chain cleavage. In conclusion, Bacillus subtilis was able to degrade polystyrene gradually, with effectiveness influenced by the size of the sample surface and the length of incubation time.