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All Journal HAYATI Journal of Biosciences Jurnal Ilmu Dasar AL KAUNIYAH Jurnal Edukasi Universitas Jember Journal of Education and Learning (EduLearn) Biogenesis: Jurnal Ilmiah Biologi Jurnal Pendidikan Biologi Indonesia JPS Unimus Jurnal Florea Phenomenon : Jurnal Pendidikan MIPA Bio Ilmi: Jurnal Pendidikan JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Jurnal Farmasi Sains dan Komunitas Jurnal Penelitian Pendidikan IPA (JPPIPA) Jurnal Bioedukatika BIOEDUKASI Journal of Science Learning Biosfer: Jurnal Pendidikan Biologi LENSA (Lentera Sains): Jurnal Pendidikan IPA Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Cetta: Jurnal Ilmu Pendidikan Jambura Edu Biosfer Journal Jurnal Mangifera Edu Majalah Biomorfologi (Biomorphology Journal) Academia Open Tafkir: Interdisciplinary Journal of Islamic Education Proceeding Biology Education Conference Pancaran Pendidikan Jurnal Pendidikan MIPA Edubiotik : Jurnal Pendidikan, Biologi dan Terapan Indonesian Journal of Science and Education
Erlia Narulita
Universitas Jember (SCOPUS ID: 56521650500)
Religion Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Library & Information Science Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Neuroscience Physics Public Health Social Sciences Other

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Journal : AL KAUNIYAH

 1 
Produksi Antibodi Poliklonal Menggunakan Protein Rekombinan RBD-spike Untuk Deteksi SARS-CoV-2 Iryani Endah Febrianti; Yayuk Fatmawati; Intan Ria Neliana; Widhi Dyah Sawitri; Erlia Narulita; Bambang Sugiharto
Al-Kauniyah: Jurnal Biologi Vol 16, No 2 (2023): AL-KAUNIYAH JURNAL BIOLOGI
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/kauniyah.v16i2.25664

Abstract

 AbstrakSARS-CoV-2 merupakan virus yang menyebabkan Coronavirus Disease 2019 (COVID-19) di seluruh dunia dan sampai saat ini kasus terbaru masih terus dilaporkan. Diagnostic test merupakan hal yang krusial untuk dikembangkan. Prinsip diagnostic test COVID-19 berbasis antigen, yaitu mendeteksi virus SARS-CoV-2 melalui respon antibodi dari penderita. Penelitian ini bertujuan untuk memproduksi antibodi poliklonal menggunakan protein rekombinan RBD-Spike untuk mendeteksi virus SARS-CoV-2 berbasis antibodi. Penelitian dimulai dengan penentuan domain RBD-Spike menggunakan pensejajaran asam amino, dan konstruksi DNA untuk RBD-Spike pada vektor ekspresi pET28a menggunakan sintetik nukleotida. Produksi protein rekombinan RBD-Spike diekspresikan pada sel bakteri Escherichia coli. Purifikasi dilakukan untuk memperoleh protein RBD-Spike dan selanjutnya digunakan sebagai antigen untuk induksi antibodi poliklonal pada kelinci. Hasil penelitian menunjukkan bahwa ekspresi protein rekombinan RBD-Spike SARS-CoV-2 memerlukan induksi IPTG 0,1 mM dan terekspresi dalam bentuk inclusion bodies dengan ukuran 39 kDa. Purifikasi protein RBD-Spike dilakukan menggunaan resin afinitas NiNTA, elektroelusi, dan dialisis. Total protein RBD-Spike yang diperoleh sebanyak 4 mL dengan konsentrasi 10 mg/mL. Analisa Ouchterlony menunjukkan bahwa antibodi poliklonal terdeteksi pada minggu kedua setelah injeksi booster dan analisa spesifitas antibodi terhadap antigen menunjukkan bahwa antibodi poliklonal dapat mendeteksi protein RBD-Spike pada konsentrasi 0,1 µg. Selanjutnya diharapkan antibodi poliklonal dapat digunakan untuk deteksi keberadaan virus SARS-CoV-2 dan dapat dikembangkan untuk kit deteksi berbasis antibodi.AbstractSARS-CoV-2 is the virus that causes Coronavirus Disease 2019 (COVID-19) worldwide and the latest cases are still being reported until now. The diagnostic test is a crucial to be developed. The principle of the antigen-based COVID-19 diagnostic test is to detect the SARS-CoV-2 virus through antibody response from the patients. This study was conducted to produce polyclonal antibodies using recombinant protein RBD-Spike. The research was carried out by determining the RBD-Spike domain using amino acid alignment and constructing the DNA of RBD-Spike to the expression vector of pET28a using nucleotide synthesis. Production of RBD-Spike recombinant protein was expressed in Escherichia coli. Purification was carried out to obtain RBD-Spike protein and used to induce polyclonal antibody ina rabbit. The results showed that the expression of RBD-Spike recombinant protein required induction of IPTG 0.1 mM and was expressed in inclusion bodies with molecular size of 39 kDa. The purification of RBD-Spike protein was carried out using resin affinity, electroelution, and dialysis. The total protein of RBD-Spike obtained was 4 mL with a concentration of 10 mg/mL. Ouchterlony analysis revealed that polyclonal antibody was detected in the second week after booster injection and analysis of antibody specificity showed that polyclonal antibodies detected RBD-Spike protein at the concentration of 0.1µg of RBD-Spike protein. Moreover, it is expected that our polyclonal antibody detect the SARS-CoV-2 virus and can be developed for antibody-based detection kits.
Bacteriophage-Based Wound Care Formulation for Diabetic Ulcers as A Growth Inhibitor of Alcaligenes faecalis Kiswara, Nur Hanifah Alim; Ludfillah, Rayyan Zhafir; Febrianti, Riska Ayu; Narulita, Erlia; Winarti, Lina
Al-Kauniyah: Jurnal Biologi Vol. 19 No. 1 (2026): AL-KAUNIYAH JURNAL BIOLOGI
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/kauniyah.v19i1.44082

Abstract

Diabetic ulcers are frequently complicated by infections from multidrug-resistant bacteria such as Alcaligenes faecalis, necessitating the development of alternative therapeutic strategies. Bacteriophage therapy presents a promising approach due to its specificity and efficacy against antibiotic-resistant pathogens. This study aimed to formulate and evaluate a bacteriophage-based topical cleanser using the phage ϕAFaV1 for treating A. faecalis infections, focusing on high antibacterial efficacy and low dermal toxicity. Three suspension formulas (F1, F2, F3) were prepared with varying chitosan concentrations (0.5; 0.75, and 1.0%) as a suspending agent. These formulations underwent physical stability testing over 28 days, including assessments of viscosity, pH, zeta potential, and homogeneity. Antibacterial efficacy was determined through inhibition zone assays. All three formulations effectively inhibited bacterial growth, forming clear zones. The F2 formula (0.75% chitosan), containing 200 µL of active phage ingredient, was identified as the optimum formulation. It demonstrated superior physical stability and the most effective bactericidal activity against A. faecalis, as confirmed by statistical analysis. These results indicate that a bacteriophage suspension stabilized with 0.75% chitosan is a highly promising and stable topical agent for combating multidrug-resistant A. faecalis in diabetic wound care.
Co-Authors .,, Rasmiyana Agatha, Nabiela Dini Agviolita, Putri Aini Meitanti Rosalina Akhmad Kadir Al Hadi, Salma Khatami Ali, Aisyah Amala Sholeha Apridza Amalia, Rachma Fadhillah Anjar Putro Utomo Anusorn Kongsawang Areerat Chuseng Aris Singgih Budiarso Bambang Sugiharto Bella, Frimita Bevo Wahono Billa, Attika Salsa Daud, Nadhiratuzzahra Alifiah Dewi, Ratna Sari Dyah Intan Prismasari Faizah Firdaus Fatmawati Ningsih Febrianti, Riska Ayu Fikri Ainur Risma Hardiyanti Oktavia Gerda Permata Aji Grahita, Amanda Claudia Ayuning HARDIAN SUSILO ADDY Hilya Wildana Sofia Holida Isnania Iir Nur Choiriya Ika Lia Novenda Ima Masafatus Sholihah Intan Ria Neliana Iryani Endah Febrianti Jekti Prihatin Kanoklada . Khairunnisa, Icha Khoirul Anam Kiswara, Nur Hanifah Alim Krisianti Ayu Monita Kuswati Kuswati laili, Mumtahana Lina Winarti Linda Kusumawati Ludfillah, Rayyan Zhafir Lum’atul Khoirot Rizki Luthfia, Syarifattul M. Amien Rais Manikya Pramudya Marditianingsih, Meilia Putri Marzuqi, Salsabila Jahroh Maulana, Alfan Irsyad muhammad rizqi nur Murtadho, Ahmad Hibban Nabiela Dini Agatha Nadyatul Ilma Indah Savira Nur Ahmad Nur Farida Nurul Aslami Nury Qurrota Ayun Pramudya Dwi Aristya Putra Purwanto, Martinus Effand Pratama Putri, Adelia Meita Qulbi, Siti Shofa Assyifa Ratna Sari Dewi Ria Yulian Rif’atul Fitri Supa’at Rohmah Jwita Sari Rusdianto Rusmala Evi Anggraeni S Suratno Singgih Bektiarso Siti Murdiyah Slamet Hariyadi Slamet Hariyadi Slamet Hariyadi Sofia, Hilya Wildana Sri Wahyuni Sri Wahyuni Sudarti Sudarti Supeno Suratno Suratno Sylvia Rimbanita Purwanto Ulin Nuha Vendi Eko Susilo Wachju Subchan Walukow, Auldry Fransje Wicaksono, Iwan Widhi Dyah Sawitri Yayuk Fatmawati Yayuk Fatmawati, Yayuk Yeni Firdayanti Yulian, Ria Zahro, Aliza