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The Effect Of Different Temperature On Survival Rate And P38 Mapk (Mytogen Activity Protein Kinase) Of Pangasius Djambal Saputra Fredo; Maheno Sri Widodo; Eric Armando
Journal of Aquaculture Development and Environment Vol 3, No 1 (2020): Journal Of Aquaculture Development And Environment
Publisher : Universitas Tidar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31002/jade.v3i1.2357

Abstract

The low survival value of seedlings is due to stress. Naturally cells in fish respond to the presence of environmental stressors by producing stress proteins such as mitogen-activated protein kinases (MAPKs). MAPKs are one of the important cellular signaling systems in fish as a response to the presence of environmental stressors. This study uses a completely randomized design method, 3 treatments 3 replications. The treatments given were (A) 28 o C temperature treatment, (B) 30 o C temperature treatment, (C) 32 o C temperature treatment, observations made were measurements of p38 mapk levels, survival and physical and chemical parameters. From observations of tissue staining (p38 MAPK) in the above table shows that the best results obtained in treatment B (30 o C) because there are many negative MAPK, so that at experience 30 o C the fish did not significant stress, and the highest survival rate was 83.3 %.
REPRODUCTIVE RESPONSE OF NILE TILAPIA (Oreochromis niloticus) AFTER INDUCED THE GONADAL SUPERNATANT OF YELLOW FIN TUNA (Thunnus albacares) tholibah mujtahidah; Maheno Sri Widodo; Abdul Rahem Faqih
Journal of Aquaculture Development and Environment Vol 1, No 1 (2018): Journal Of Aquaculture Development And Environment
Publisher : Universitas Tidar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31002/jade.v1i1.1015

Abstract

THE EFFECT OF DIFFERENT TEMPERATURE TOWARD THE SURVIVAL RATE AND SPECIFIC GROWTH RATE OF THE SILVER ARWANA FISH (Osteoglossum bicirrhosum) Eric Armando; Maheno Sri Widodo
Journal of Aquaculture Development and Environment Vol 1, No 1 (2018): Journal Of Aquaculture Development And Environment
Publisher : Universitas Tidar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31002/jade.v1i1.1016

Abstract

The business of arwana hatchery is now grew up. One of the main factors which can increase the successfulness of arwana hatchery is water quality parameter, such temperature. This research was held on February to April 2014 in BBI Punten, Batu. The silver arwana hatchery was from “MINA KARYA KOI CENTER” Sleman witch its height 5,3 cm and weight 0,5 gr. It was treated in the aquarium 100x40x50 cm3 with its density 10 fry/aquarium. The treatment which given such (A) the temperature is 26oC, (B) the temperature is 28oC, and (C) the temperature is 30oC in 5 time repeated. The silver arwana hatchery which treated in thetemperature 26oC has the survival rate as 78% and the lowest score for temperature 30oC treatment is 20%. In 26oC temperature, the average of height growth is -2,7 cm and the average of weight growth in a day is 2,05%.
Manajemen Kesehatan Larva Kerapu Cantang (Epinephelus fuscoguttatus x Epinephelus lanceolatus) di BBRBLPP Gondol Muh. Sulaiman Dadiono; Maheno Sri Widodo; Emyliana Listiowati; Baruna Kusuma
Samakia : Jurnal Ilmu Perikanan Vol 13 No 2 (2022): Samakia: Jurnal Ilmu Perikanan
Publisher : Faculty of Science and Technology University Ibrahimy

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (260.844 KB) | DOI: 10.35316/jsapi.v13i2.1273

Abstract

Larval health management is a serious problem faced by hatcheries of cantang grouper (E. fuscoguttatus x E. lanceolatus) in order to avoid large financial losses due to larval death. Health Management of cantang grouper larvae includes larval rearing, larval feed management, larval disease control and water quality management. Primary data collection methods are active participation, observation and direct interviews. Secondary data collection by means of literature study. Cantang grouper larvae were reared in the hatchery to control the temperature and condition of the larvae. The ponds were sterilized with 3-5 ppm chlorine in the morning and 3-5 ppm thiosulfate in the afternoon. Then rinsed so that no residual chlorine. Larvae were reared from day 1 to day 45. Larvae were fed from 2 days old with Nannochloropsis occulata. Rotifers were given when the larvae were 2-3 days old with a density of 5 individuals/ml. Artemia was given when the larvae were 18 days old until the age of 40 days. Pellets are given when the larvae are 8-10 days old with a pellet size of level 1. The pellet size continues to increase according to the larval mouth opening until harvest. The disease that attacks the larvae is Viral Nervous Necrosis. The characteristics of larvae infected with VNN are reduced appetite, weak movement, larvae float on the surface and lie on the bottom of the pond. The bacteria that often attack larvae is Vibrio alginolyticus. Vibrio alginolyticus can be treated using drugs from natural ingredients. The temperature in the larval pond is 28-30oC and the salinity is 34-35 ppt. This temperature and salinity are still quite good for grouper larvae to live.
Pengaruh Pemberian Larutan Daun Pepaya Pada Lele yang Diinfeksi Aeromonas hydrophila Ditinjau dari Histopatologi Insang, Kulit, dan Otot Dewi Susylowati; Sri Andayani; Maheno Sri Widodo
Proceedings Series on Physical & Formal Sciences Vol. 4 (2022): Prosiding Seminar Nasional Fakultas Pertanian dan Perikanan
Publisher : UM Purwokerto Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30595/pspfs.v4i.514

Abstract

Histopatologi dapat memberikan gambaran perubahan atau kelainan pada level jaringan. Parameter utama pada penelitian ini adalah histopatologi insang, kulit, dan otot. Penelitian menggunakan dua kontrol yaitu kontrol normal (tanpa infeksi dan tanpa perlakuan daun pepaya) dan kontrol infeksi (tanpa paparan larutan daun pepaya). Lima perlakuan yang digunakan yaitu pengobatan dengan paparan larutan daun pepaya dengan dosis 10, 15, 20, 25 dan 30 mg/mL. Hasil penelitian menunjukkan insang dan kulit ikan normal tidak mengalami hiperplasia. Otot ikan normal terlihat kompak dan rapat antara sel yang satu dengan yang lainnya. Insang dan kulit ikan yang terinfeksi mengalami kerusakan jaringan. Kerusakan jaringan juga terjadi pada perlakuan pengobatan paparan larutan daun pepaya berupa hiperplasia dengan tingkat radang, degenarasi, dan nekrosis yang berbeda-beda. Hasil skoring ikan terinfeksi tanpa pengobatan memiliki memiliki nilai yang hampir sama dengan perlakuan pengobatan pada paparan larutan daun pepaya dosis 25 dan 30 mg/mL. Hal ini diakrenakan larutan dnegan konsentrasi tinggi memberikan efek toksik. Sebaliknya, dosis 10 mg/mL kurang efektif untuk pengobatan karena bersifat bakteriostatik yaitu hanya menahan pergerakan bukan membunuh bakteri. Dosis yang tepat untuk membunuh bakteri dan tidak bersifat toksik adalah pengobatan dengan paparan larutan daun pepaya dosis 15 dan 20 mg/mL. Hal tersebut didukung oleh kelulushidupan ikan lele.
Dosage Determination of Galangal Extract (Alpinia purpurata) Through LC50-96 Method on Zebrafish Larvae (Danio rerio) as a Model Muhammad Azmi Amanullah; Maheno Sri Widodo; Abdul Raheem Faqih
Journal of Applied Science, Engineering, Technology, and Education Vol. 4 No. 2 (2022)
Publisher : PT Mattawang Mediatama Solution

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (325.861 KB) | DOI: 10.35877/454RI.asci1138

Abstract

Galangal (Alpinia purpurata) is a herb and medicine plant that is found in Southeast Asia including Thailand, Malaysia, and Indonesia. It is known to have health benefits for its flavonoid, phytosterol, and phenols that are anti-oxidant, anti-cancer, and anti-fungal properties. The phytosterol also can be used for sex hormones production.The exploration of its uses in aquaculture are not yet fully understand. In this LC50-96 experiments were tested for its optimum dosage used in aquaculture by using zebrafish larvae (Danio rerio) as a model. The optimum dosage in the experiments were between 1—31,83 ppm for ethanolic extract of galangal and <0,83 ppm for methanolic extract of galangal. Its uses in aquaculture must be further explored by its effect in fishes behaviors or by the histology of the organs.
Effect of 17β-Estradiol on Feminization, Growth Rate and Survival Rate of Pacific White Shrimp (Litopenaeus vannamei, Boone 1931) Postlarvae I Nengah Gde Sugestya; Maheno Sri Widodo; Agoes Soeprijanto
The Journal of Experimental Life Science Vol. 8 No. 1 (2018)
Publisher : Postgraduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1036.449 KB) | DOI: 10.21776/ub.jels.2018.008.01.06

Abstract

This study was therefore aimed to determine the effect of different concentration of estrogen hormone, 17β-estradiol (E2) on feminization for the production of all female, growth rate and survival rate of Pacific white shrimp (Litopenaeus vannamei) postlarvae (PL). L. vannamei PL1 were stocked randomly at a density of 10 ind.L-1 into a 10 L of seawater for each experimental flask with three replicates each. PL1 was immersed in seawater containing 0.5, 1.0 and 1.5 mg.L-1 estrogen hormone, E2 for 4 hours. The experiment was continued for 30 days for larval rearing. At the termination of experiment, the specimens in each treatment groups were weighed and measured individually for their wet body weight (BW) and total length (TL) for the mean weight gain, length gain, and the specific growth rate (SGR) estimation. The mean sex ratio values of male to female postlarvae obtained from control till the highest E2 hormone concentration of 0, 0.5, 1.0 and 1.5 mg.L-1 were 1:1, 1:2, 1:9 and 0:1 respectively. The weight gain and length gain were 40605% and 606% for control, 46310% and 647% for 0.5 mg.L-1 concentration, 49310% and 663% for 1.0 mg.L-1 concentration and 45048% and 628% for 1.5 mg.L-1. The mean SGR BW and SGR TL for control till the highest E2 hormone concentration of 0, 0.5, 1.0 and 1.5 mg.L-1 were 20.00 and 6.51, 20.46 and 6.70, 20.67 and 6.77 and 20.37 and 6.62 respectively. The mean survival rate for E2 hormone concentration of 0, 0.5, 1.0 and 1.5 mg.L-1 were 52.67%, 54.33%, 54.67% and 52.33% respectively. The study shows that while the concentration of E2 hormone increased, the female sex ratio of L. vannamei PL also increased but the growth rate and survival rate decreased at concentration of 1.5 mg.L-1. Thus the optimum concentration of this hormone usage was 1.0 mg.L-1 immersed for 4 hours. Keywords: 17β-estradiol, feminization, growth rate, Litopenaeus vannamei, postlarvae, survival rate.
The Effect of Difference Temperature on Cortisol, Glucose and Glycogen level of Uceng Fish (Nemacheilus fasciatus) Harun Wijaya; Maheno Sri Widodo; Agoes Soeprijanto
The Journal of Experimental Life Science Vol. 9 No. 1 (2019)
Publisher : Postgraduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (557.651 KB) | DOI: 10.21776/ub.jels.2019.009.01.01

Abstract

Nemacheilus is one of the genera of freshwater fish. The existence of Uceng fish (Nemacheilus fasciatus) in public waters is increasingly rare. Changes in water temperature in the maintenance medium will affect the physiological processes of fish. The purpose of this study was to analyze physiological responses (blood glucose levels, cortisol, and glycogen hormones) of Uceng Fish (N. fasciatus) which were incubated at different temperatures and find out the initial time of Uceng fish adaptation to its new environment. Total of 200 fish were collected from wild catches in the Lekso River, Blitar. Fish tissue collection was carried out to test the profile of glucose, cortisol, and glycogen. The research activities at this stage were carried out by raising the Uceng fish from nature to a cultivation container in an aquarium of size 50 x 30 x 30 cm that given the different temperatures (20°C, 24°C, 28°C and 32°C), and each aquarium is filled with 10 Uceng fish and will be kept for 14 days. ELISA method was used to quantify the parameter. The result of this study showed highest peak of cortisol levels and blood glucose levels was achieved in the treatment of 24°C on the 10th day and the lowest in the treatment temperature of 28°C on the 6th day. The highest glycogen peak was reached at 32°C on day 10 and the lowest peak was reached at 28°C on day 2. We conclude that temperature affects the physiological response (cortisol, blood glucose, and glycogen) of Uceng fish (N. fasciatus). High levels of cortisol and blood glucose indicate stressful fish. Keywords: Fish, Nemacheilus fasciatus, Physiological, Temperature, Uceng.
The Effect of Methyltestosterone Hormone Immersion on Male Formation in Gourami Larvae (Osphronemus goramy Lacepè¨de, 1801) Tatang Tatang; Maheno Sriwidodo; Harsuko Riniwati
The Journal of Experimental Life Science Vol. 10 No. 3 (2020)
Publisher : Postgraduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2020.010.03.03

Abstract

The methyltestosterone hormone is a synthetic hormone of androgen and has been used to obtain the juveniles of male mono-sexual fish such as Tetra Congo, Tilapia, and Betta. The methyltestosterone use on gourami has not maximized. It because there is no data on the exact age of larvae to produce maximum male mono-sexual juveniles, so it is necessary to do research on the effect of methyltestosterone hormone on the age of gourami larvae on the success of male mono-sexual formation and get the right larval period to obtain maximum survival gourami. The methods of this research were conducted using a completely randomized design with four treatments of the age of gourami larvae aged ten days, 15 days, 20 days, and 25 days. Each procedure was repeated three times. Data analysis used ANOVA analysis and LSD test. The larvae are soaked in a solution containing 5 ppm of hormones for 24 hours. The results showed that the administration of the hormone methyltestosterone to gourami larvae (Osphronemus goramy Lacepè¨de, 1801) with different ages had a very significant effect (P <0.01) on male sex formation with the highest average percentage of males obtained in treatment B (15-day larvae age) that is equal to 82.33% and the lowest in the treatment of larval period of 10 and 20 days is 74.00%. Keywords: Gourami, hormone, juvenile, methyltestosterone, Osphronemus goramy.
Perbedaan Padat Penebaran Terhadap Kualitas Air Ikan Nila Merah (Oreochormis sp) Pada Sistem Budikdamber Vina Nur Nadiro; Sri Andayani; Maheno Sri Widodo; Nurhalisa Nurhalisa
Jurnal Penelitian Pendidikan IPA Vol 9 No 4 (2023): April
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v9i4.3236

Abstract

The problems of fish and plant cultivation are limited land, decreasing quality and quantity of water for human needs, food sources, and increasing population on earth. Optimization of fish farming with high stocking densities accompanied by high feeding will cause accumulation of organic matter in the culture containers which will worsen the quality of the rearing water which will ultimately have an impact on the physiological conditions, survival and growth of fish. One of the technologies for growing vegetables, fruit and fish farming that has been developed to overcome these obstacles is the Budikdamber system with red tilapia organisms and water spinach plants. The purpose of this study was to determine the quality of water with different stocking densities in the Budikdamber system with water spinach and without water spinach. The experimental design used a factorial complete randomized design with density treatment (A (2 fish/10L), B (4 fish/10L), C (6 fish/10L), D(8 fish/10L) and system (budikdamber with water spinach) (a) and without water spinach (b)). The results showed that temperature parameters ranged from 22.4 - 30.6°C, pH ranged from 6.4 - 8.9, and DO ranged from 1.07 - 16.4 mg/l. Parameters of temperature, pH, and DO are in a range that is not in accordance with optimum conditions (temperature = 25-32°C, pH = 6.5 – 8, and DO ≥3 ), but can still be tolerated by tilapia so that fish tilapia still survive. Water spinach plants can reduce ammonia so that the ammonia value in the treatment using kale decreased from week 1 to 4 and conversely, for budikdamber without water spinach, it increased from week 1 to week 4. The highest SR value was found in the Ba treatment (4 fish/10 liters) with water spinach is 91.67%.
Co-Authors Abdul Raheem Faqih Abdul Rahem Faqih Agoes Soeprijanto, Agoes Aida Sartimbul Aida Sartimbul Akhsan Fikrillah Paricahya Amalia Ayuk Riyadini Amalia, Elok Andi Masriah Andik Isdianto Andra Rejekineng Rahayu Andra Rejekineng Rahayu Rahayu Andra Rejekining Rahayu Angga Wira Perdana Anik Martinah Hariati Ariyani, Destia Fitri Arning Wilujeng Ekawati Ating Yuniarti Bayu Kusuma Bayu Kusuma Bela Fatma Hani Ayu Lestari Dedi Pardiansyah Dewa Gede Raka Wiadnya Dewi Susylowati Diana Arfiati Eka Fitria Febriani Emyliana Listiowati, Emyliana Eric Armando Fani Fariedah Fariedah, Fani Fisma Josara Apriliyanti Fitri Sil Valen Frits Jamlaay, Frits Harahap, Muhammad Alfiandi Rachmad Hariati, Anik Martinah Harsuko Riniwati Harun Wijaya I Nengah Gde Sugestya Ifa Sufaichusan Ir. Mulyanto, MS Ir. Mulyanto, MS, Ir. Mulyanto, Irfanov Hafiz Kiki Nur Azam Kholil Kurniasari, Reni Dyah Yuni Kusuma, Baruna Kusuma, Bayu Kusuma, Wahyu Endra Lugu Tri Handoko Lugu Tri Handoko Handoko Lutviana N.D Marsoedi Marsoedi Marsoedi Marsoedi Mimit Primyastanto Mimit Primyastanto Mohamad Fadjar Muh. Sulaiman Dadiono Muhammad Azmi Amanullah Muhammad Dailami, Muhammad Muhammad Fadjar Mujtahidah, Tholibah Nadia Dara Panggita Wati Nadia Dara Panggita Wati Nadiro, Vina Nur Niken Hendrakusma Wardani Nurhabib, Asro Nurhalisa Nurhalisa Pratama, Rakhmad Ndaru R Adharyan Islamy Ramadhan, Surya Dewa Risnita Tri Utami Risnita Tri Utami Utami Rizal Akbar Hutagalung, Rizal Akbar Rohmah Widiastuti Rudianto Rudy Wijaya Samsudin Hariyanto Saputra Fredo Seftiawan Samsu Rijal Septiana Sri Astuti Sinaga, Sorbakti Soemarno Soemarno Soko Nuswantoro Sorbakti Sinaga Sri Andayani Sri Andayani Sudjatmiko, Sudjatmiko Sufaichusan, Ifa Suharun Martudi Supriatna Tatang Tatang Taufik Budhi Pramono Taufik Budhi Pramono Tholibah Mujtahidah tholibah mujtahidah Veryl Hasan Vina Nur Nadiro Wahida Kartika Sari Wahyu Endra Kusuma Yanuhar, Uun Yuni Kilawati Yunita Maimunah