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REPRODUCTIVE RESPONSE OF NILE TILAPIA (Oreochromis niloticus) AFTER INDUCED THE GONADAL SUPERNATANT OF YELLOW FIN TUNA (Thunnus albacares) tholibah mujtahidah; Maheno Sri Widodo; Abdul Rahem Faqih
Journal of Aquaculture Development and Environment Vol 1, No 1 (2018): Journal Of Aquaculture Development And Environment
Publisher : Universitas Tidar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31002/jade.v1i1.1015

Abstract

THE EFFECT OF DIFFERENT TEMPERATURE TOWARD THE SURVIVAL RATE AND SPECIFIC GROWTH RATE OF THE SILVER ARWANA FISH (Osteoglossum bicirrhosum) Eric Armando; Maheno Sri Widodo
Journal of Aquaculture Development and Environment Vol 1, No 1 (2018): Journal Of Aquaculture Development And Environment
Publisher : Universitas Tidar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31002/jade.v1i1.1016

Abstract

The business of arwana hatchery is now grew up. One of the main factors which can increase the successfulness of arwana hatchery is water quality parameter, such temperature. This research was held on February to April 2014 in BBI Punten, Batu. The silver arwana hatchery was from “MINA KARYA KOI CENTER” Sleman witch its height 5,3 cm and weight 0,5 gr. It was treated in the aquarium 100x40x50 cm3 with its density 10 fry/aquarium. The treatment which given such (A) the temperature is 26oC, (B) the temperature is 28oC, and (C) the temperature is 30oC in 5 time repeated. The silver arwana hatchery which treated in thetemperature 26oC has the survival rate as 78% and the lowest score for temperature 30oC treatment is 20%. In 26oC temperature, the average of height growth is -2,7 cm and the average of weight growth in a day is 2,05%.
Manajemen Kesehatan Larva Kerapu Cantang (Epinephelus fuscoguttatus x Epinephelus lanceolatus) di BBRBLPP Gondol Muh. Sulaiman Dadiono; Maheno Sri Widodo; Emyliana Listiowati; Baruna Kusuma
Samakia : Jurnal Ilmu Perikanan Vol 13 No 2 (2022): Samakia: Jurnal Ilmu Perikanan
Publisher : Faculty of Science and Technology University Ibrahimy

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (260.844 KB) | DOI: 10.35316/jsapi.v13i2.1273

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Larval health management is a serious problem faced by hatcheries of cantang grouper (E. fuscoguttatus x E. lanceolatus) in order to avoid large financial losses due to larval death. Health Management of cantang grouper larvae includes larval rearing, larval feed management, larval disease control and water quality management. Primary data collection methods are active participation, observation and direct interviews. Secondary data collection by means of literature study. Cantang grouper larvae were reared in the hatchery to control the temperature and condition of the larvae. The ponds were sterilized with 3-5 ppm chlorine in the morning and 3-5 ppm thiosulfate in the afternoon. Then rinsed so that no residual chlorine. Larvae were reared from day 1 to day 45. Larvae were fed from 2 days old with Nannochloropsis occulata. Rotifers were given when the larvae were 2-3 days old with a density of 5 individuals/ml. Artemia was given when the larvae were 18 days old until the age of 40 days. Pellets are given when the larvae are 8-10 days old with a pellet size of level 1. The pellet size continues to increase according to the larval mouth opening until harvest. The disease that attacks the larvae is Viral Nervous Necrosis. The characteristics of larvae infected with VNN are reduced appetite, weak movement, larvae float on the surface and lie on the bottom of the pond. The bacteria that often attack larvae is Vibrio alginolyticus. Vibrio alginolyticus can be treated using drugs from natural ingredients. The temperature in the larval pond is 28-30oC and the salinity is 34-35 ppt. This temperature and salinity are still quite good for grouper larvae to live.
Pengaruh Pemberian Larutan Daun Pepaya Pada Lele yang Diinfeksi Aeromonas hydrophila Ditinjau dari Histopatologi Insang, Kulit, dan Otot Dewi Susylowati; Sri Andayani; Maheno Sri Widodo
Proceedings Series on Physical & Formal Sciences Vol. 4 (2022): Prosiding Seminar Nasional Fakultas Pertanian dan Perikanan
Publisher : UM Purwokerto Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30595/pspfs.v4i.514

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Histopatologi dapat memberikan gambaran perubahan atau kelainan pada level jaringan. Parameter utama pada penelitian ini adalah histopatologi insang, kulit, dan otot. Penelitian menggunakan dua kontrol yaitu kontrol normal (tanpa infeksi dan tanpa perlakuan daun pepaya) dan kontrol infeksi (tanpa paparan larutan daun pepaya). Lima perlakuan yang digunakan yaitu pengobatan dengan paparan larutan daun pepaya dengan dosis 10, 15, 20, 25 dan 30 mg/mL. Hasil penelitian menunjukkan insang dan kulit ikan normal tidak mengalami hiperplasia. Otot ikan normal terlihat kompak dan rapat antara sel yang satu dengan yang lainnya. Insang dan kulit ikan yang terinfeksi mengalami kerusakan jaringan. Kerusakan jaringan juga terjadi pada perlakuan pengobatan paparan larutan daun pepaya berupa hiperplasia dengan tingkat radang, degenarasi, dan nekrosis yang berbeda-beda. Hasil skoring ikan terinfeksi tanpa pengobatan memiliki memiliki nilai yang hampir sama dengan perlakuan pengobatan pada paparan larutan daun pepaya dosis 25 dan 30 mg/mL. Hal ini diakrenakan larutan dnegan konsentrasi tinggi memberikan efek toksik. Sebaliknya, dosis 10 mg/mL kurang efektif untuk pengobatan karena bersifat bakteriostatik yaitu hanya menahan pergerakan bukan membunuh bakteri. Dosis yang tepat untuk membunuh bakteri dan tidak bersifat toksik adalah pengobatan dengan paparan larutan daun pepaya dosis 15 dan 20 mg/mL. Hal tersebut didukung oleh kelulushidupan ikan lele.
Perbedaan Padat Penebaran Terhadap Kualitas Air Ikan Nila Merah (Oreochormis sp) Pada Sistem Budikdamber Vina Nur Nadiro; Sri Andayani; Maheno Sri Widodo; Nurhalisa Nurhalisa
Jurnal Penelitian Pendidikan IPA Vol 9 No 4 (2023): April
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v9i4.3236

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The problems of fish and plant cultivation are limited land, decreasing quality and quantity of water for human needs, food sources, and increasing population on earth. Optimization of fish farming with high stocking densities accompanied by high feeding will cause accumulation of organic matter in the culture containers which will worsen the quality of the rearing water which will ultimately have an impact on the physiological conditions, survival and growth of fish. One of the technologies for growing vegetables, fruit and fish farming that has been developed to overcome these obstacles is the Budikdamber system with red tilapia organisms and water spinach plants. The purpose of this study was to determine the quality of water with different stocking densities in the Budikdamber system with water spinach and without water spinach. The experimental design used a factorial complete randomized design with density treatment (A (2 fish/10L), B (4 fish/10L), C (6 fish/10L), D(8 fish/10L) and system (budikdamber with water spinach) (a) and without water spinach (b)). The results showed that temperature parameters ranged from 22.4 - 30.6°C, pH ranged from 6.4 - 8.9, and DO ranged from 1.07 - 16.4 mg/l. Parameters of temperature, pH, and DO are in a range that is not in accordance with optimum conditions (temperature = 25-32°C, pH = 6.5 – 8, and DO ≥3 ), but can still be tolerated by tilapia so that fish tilapia still survive. Water spinach plants can reduce ammonia so that the ammonia value in the treatment using kale decreased from week 1 to 4 and conversely, for budikdamber without water spinach, it increased from week 1 to week 4. The highest SR value was found in the Ba treatment (4 fish/10 liters) with water spinach is 91.67%.
Effect of Dosages and Temperatures on Simalungun Red Ginger (Zingiber officinale var. Rubrum) Ethanol Extract Dipping on Masculinization of Zebra Fish (Danio rerio) as a Model Fish Eka Fitria Febriani; Maheno Sri Widodo; Abdul Rahem Faqih
Jurnal Ilmiah Perikanan dan Kelautan Vol. 15 No. 1 (2023): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v15i1.38271

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Highlight Research Successive administration of Ginger ethanol extract to male individuals was shown to be able to produce a significant increase in sperm count, motility, viability and morphology Ethanol extract of ginger has androgenic activity which is proven to be able to increase the concentration of testosterone in serum, which functions to control the process of spermatogenesis, maintain Sertoli cells and play a role in determining the quality of spermatozoa in male organisms Giving ginger ethanol extract for 30 consecutive days to male individuals showed significant results so that ginger extract was thought to have the potential to improve male characteristics Ginger extract is potentially useful in improving healthy sperm characteristics and management of male infertility especially in those with low sperm counts   Abstract Ornamental fish are known to be easily cultivated in aquarium or pond. Many people prefer male (MF) over female fish (FF) due to their aesthetic value and productivity which lead to better pricing. The aim of this study was to determine the effect of Simalungun Red Ginger (Zingiber officinale var. Rubrum) (SRG) on masculinization. This study used dipping method which was carried out using Simalungun Red Ginger (Zingiber officinale var. Rubrum) (SRG). The temperature treatments used in this study were 28°C and 32°C. For this research, an experimental study with a Completely Randomized Factorial Design (CRFD) method that had two independent variables such as different dosages and temperatures was used. From five dosages (0 mg/L; 5 mg/L; 10 mg/L; 15 mg/L; 20 mg/L) and three temperature treatments (ambient temperature (about 25°C; 28°C; 32°C), it was found that the highest male percentage of primary sexuality (88.33%), secondary sexuality (85%), and highest total testosterone levels (TL) (1.986 ng/L) were at the treatments with the highest dosages and temperature, while for the highest survival rate were at almost in all B treatments (5 mg/L) and b levels (28°C), in which the survival rate was 100%. For all of these results above, the results were significant.
Pendugaan Fekunditas Induk Betina Ikan Nilem (Osteochilus vittatus) berdasarkan Karakter Morfometrik Ramadhan, Surya Dewa; Widodo, Maheno Sri; Ariyani, Destia Fitri; Pratama, Rakhmad Ndaru; Dailami, Muhammad
ACROPORA: Jurnal Ilmu Kelautan dan Perikanan Papua Vol 7 No 2 (2024): ACROPORA: Jurnal Ilmu Kelautan dan Perikanan Papua
Publisher : Cenderawasih University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31957/acr.v7i2.4389

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Ikan nilem merupakan ikan air tawar asli Indonesia yang persebaranya hanya di daerah Jawa Barat. Induk betina ikan nilem memiliki jumlah telur yang bervariasi sehingga perlu dilakukan seleksi. Seleksi yang dapat dilakukan pada induk betina ikan nilem yaitu dengan cara melihat karakter morfometrik. Sampai saat ini penelitian tentang ikan nilem khususnya yang berkaitan dengan reproduksi juga masih sangat jarang dilakukan. Tujuan dilaksanakanya penelitian ini adalah untuk menganalisis hubungan dan mengetahui karakter morfometrik yang paling berhubungan terhadap fekunditas. Metode yang digunakan dalam penelitian iniyaitu deskripstif komparatif dengan pendekatan kuantitatif. Parameter yang diamati meliputi panjang standar, tinggi badan, lingkar tubuh, dan bobot tubuh. Kelompok sampel ikan uji diukur berdasarkan panjang standar yang telah ditentukan, yaitu 06,1-09 cm, 09,1-12 cm, 12,1-15 cm, 15,1- 18 cm, 18,1-21 cm. Hasil penelitian menunjukkan bahwa morfometrik berpengaruh terhadap fekunditas. Karakter morfometrik terhadap fekunditas diperoleh hasil tertinggi pada lingkar tubuh dengan fekunditas sebesar 99%terhadap fekunditas yang dihasilkan pada induk betina ikan nilem. Faktor yang dapat mempengaruhi jumlah fekunditas diantaranya yaitu umur ikan, lingkungan, makanan, dan diameter ikan. Diameter ikan sangat berpengaruh terhadap jumlah fekunditas yang di hasilkan, semakin besar diameter telur dalam suatu gonad maka akan semakin sedikit jumlah telur yang dihasilkan.
Evaluation of PMSG (Oodev®) application on hepatosomatic and gonadosomatic index of snakehead fish Hutagalung, Rizal Akbar; Widodo, Maheno Sri; Faqih, Abdul Rahem
Jurnal Akuakultur Indonesia Vol. 14 No. 1 (2015): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2996.134 KB) | DOI: 10.19027/jai.14.24-29

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ABSTRACT Snakehead fish (Channa striata) is a freshwater fish commodities that have high economic value, while their needs depend on natural catching. In general, hormonal induction commonly used for the efficiency of female parent utilization and enhancement quality and quantity of fish fry. One of the hormones that can be used is pregnant mare serum gonadotropin (PMSG) and antidopamine with trademark Oodev®. These hormones contain many elements follicle stimulating hormone (FSH) which can trigger the early stages of gonad maturity. The purpose of this research was to determine the effectiveness of hormone PMSG on gonadosomatic index (GSI) and hepatosomatic index (HSI). The size of female fish was 30−40 cm, weighing of 500−700 g, adapted in the aquarium and then induced with different doses of PMSG, namely: treatment A dose: 0.75 mL/kg; B: 1.0 mL/kg; C: 1.25 mL/kg; D: 1.5 mL/kg and be repeated three times. Fishes were then reared up to 72 hours to determine the development of GSI and HSI. The results showed that the best treatment was at dose of 1.25 mL/kg with GSI and HSI values of 1.37% and 3.35%, respectively. Keywords: PMSG, GSI, HSI, snakehead fish  ABSTRAK Ikan gabus (Channa striata) merupakan salah satu komoditas air tawar yang mempunyai nilai ekonomi tinggi. Saat ini pemenuhan kebutuhan hanya bergantung pada hasil penangkapan dari alam. Pada umumnya cara pemijahan buatan dengan induksi hormon dilakukan untuk efisiensi penggunaan induk serta peningkatan kualitas dan kuantitas benih ikan. Salah satu hormon yang dapat digunakan adalah pregnant mare serum gonadotropin (PMSG) dan antidopamin dengan merk dagang Oodev®. Hormon-hormon tersebut banyak mengandung unsur follicle stimulating hormon (FSH) yang dapat memicu kematangan gonad tahap awal. Tujuan dari penelitian ini adalah untuk menguji efektivitas hormon PMSG terhadap nilai indeks gonadosomatik (IGS) dan indeks hepatosomatik (IHS). Induk ikan gabus yang digunakan berukuran 30−40 cm dengan bobot 500–700 g, diaklimatisasikan di akuarium kemudian diinduksi hormon PMSG dengan perlakuan dosis A: 0,75 mL/kg; B: 1,0 mL/kg; C: 1,25 mL/kg; D: 1,5 mL/kg dan dilakukan pengulangan sebanyak tiga kali. Selanjutnya ditunggu hingga 72 jam untuk mengevaluasi perkembangan IGS dan IHS nya. Hasil penelitian menunjukkan bahwa perlakuan terbaik adalah dosis 1,25 ml/kg dengan nilai rata-rata IHS 1,37% dan IGS 3,35%, Kata kunci: PMSG, IGS, IHS, ikan gabus 
Ovulation time and ovulated eggs count of sneakehead Channa striata induced by prostaglandin 2α with different doses Jamlaay, Frits; Widodo, Maheno Sri; Faqih, Abd. Rahem
Jurnal Akuakultur Indonesia Vol. 15 No. 1 (2016): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3067.389 KB) | DOI: 10.19027/jai.15.89-92

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ABSTRACT Snakehead fish Channa striata is one of freshwater commodity which has high economic value. Nowadays, snakehead fish stock mostly comes from wild capture. Artificial spawning with hormonal induction is usually used to improve broodstock efficiency, as well as the quality and quantity of fish seed production. Purpose of this study was to determine the effectiveness of prostaglandin 2α (PGF2α) hormone on ovulation time and count of ovulated eggs. This experiment used 30–40 cm broodstock with 600–900 g body weight. Fishes were acclimated for one week in a container and then injected with different doses of PGF2α, namely: control without PGF2α (P1), 0.5 mL/kg (P2); 0.7mL/kg (P3); and 0.9 mL/kg (P4). Each treatment consisted of three fishes as replication. Ovulation time was observed until 72 hours post injection. The results showed that PGF2α injected broodstoks ovulated faster (9.17–12.24 hours post injection) than control P1 (22.67 hours), while among PGF2α injected fishes were the same. Furthermore, count of ovulated eggs from PGF2α induced broodstocks were higher than control P1, and the highest (2,860 eggs) was obtained in treatment 0.9 mL/kg. Thus, PGF2α at dose of 0.9 mL/kg can be used to induce eggs ovulation of snakehead fish and to increase count of ovulated eggs. Keywords: PGF2α, ovulation time, ovulated eggs count, Channa striata  ABSTRAK Ikan gabus Channa striata merupakan salah satu komoditas air tawar yang mempunyai nilai ekonomi tinggi. Saat ini, pemenuhan kebutuhan hanya bergantung pada hasil penangkapan di alam. Cara pemijahan buatan dengan induksi hormon biasanya digunakan untuk efisiensi penggunaan induk serta peningkatan kualitas dan kuantitas benih ikan yang dihasilkan. Tujuan dari penelitian ini adalah untuk menguji efektivitas hormon prostaglandin 2α (PGF2α) terhadap waktu ovulasi dan jumlah telur ikan gabus yang diovulasikan. Penelitian ini menggunakan induk ikan gabus berukuran 30–40 cm dengan bobot tubuh 600–900 g. Ikan diadaptasikan di wadah penelitian selama seminggu, kemudian diinjeksi hormon PGF2α dengan perlakuan hormon dengan dosis berbeda, yaitu: kontrol tanpa hormon (P1), 0,5 mL/kg (P2); 0,7 mL/kg (P3); dan 0,9 mL/kg (P4). Setiap perlakuan terdiri atas tiga ekor induk sebagai ulangan. Waktu ovulasi diamati sampai dengan 72 jam pascasuntik. Hasil penelitian menunjukkan bahwa induk yang disuntik PGF2α memijah lebih cepat (9,17–12,24 jam pascasuntik) daripada kontrol (22,67 jam), sedangkan antarperlakuan hormon PGF2α tidak berbeda. Selanjutnya, jumlah telur diovulasi dari induk ikan gabus yang disuntik PGF2α berjumlah lebih banyak daripada kontrol. Hasil tertinggi (2.860 butir) diperoleh dari perlakuan 0,9 mL/kg.  Dengan demikian, hormon PGF2α dosis 0,9 mL/kg dapat digunakan untuk mempercepat waktu ovulasi ikan gabus, dan meningkatkan jumlah telur yang diovulasikan. Kata kunci: PGF2α, waktu ovulasi, jumlah telur diovulasi, Channa striata 
The Impact of Artificial Barriers on the Varuna litterata Migration Route in the Lower Serayu River, Central Java and Its Molecular Identification Sinaga, Sorbakti; Maimunah, Yunita; Ekawati, Arning Wilujeng; Widodo, Maheno Sri
Journal Omni-Akuatika Vol 20, No 1 (2024): Omni-Akuatika May
Publisher : Fisheries and Marine Science Faculty - Jenderal Soedirman University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.oa.2024.20.1.1140

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The Serayu River is one of the longest rivers in Central Java. Dams built in the lower reaches of the Serayu River are thought to block the migration paths of fish and aquatic macrocrustaceans. This study aimed to determine the effect of dam on Varuna litterata migration and its molecular identification. This research was conducted using the observation method. Migration observations were made on vertical paths while molecular identification of V. litterata in the lower reaches of the Serayu River was compared with existing data in GenBank. The study covered the freshwater area above the Serayu Dam, the open area with freshwater below the Serayu Dam, and the open area with brackish water at the estuary of the Serayu River, with a study length of 33.37 km. The results showed that the presence of dams in the lower reaches of the Serayu River inhibited the migration pattern of V. litterata. The vertical shape of the dam causes only a few V. litterata to migrate to the top of the dam. The dam in the lower reaches of the Serayu River resulted in not many V. litterata being able to migrate to the top of the dam, so there were very few V. litterata found in the population upstream of the dam (115 ind). The results of molecular identification of V. litratata using the cytochrome C oxidase subunit I (COI) gene show that this crab is 100% the same as the data in GenBank and is closely related to Varuna yui and other Varunidae crab species.Keywords: Dams, Molecular, Migration, Serayu River, Varuna litterata
Co-Authors Abdul Rahem Faqih Agoes Soeprijanto, Agoes Aida Sartimbul Aida Sartimbul Aida Sartimbul Amalia Ayuk Riyadini Amalia, Elok Amanullah, Muhammad Azmi Andi Masriah Andik Isdianto Andra Rejekineng Rahayu Rahayu Andra Rejekining Rahayu Angga Wira Perdana Anik Martinah Hariati Apriliyanti, Fisma Josara Ariyani, Destia Fitri Arning Wilujeng Ekawati Ating Yuniarti Bayu Kusuma Bayu Kusuma Dedi Pardiansyah Dewa Gede Raka Wiadnya Dewi Susylowati Diana Arfiati Eka Fitria Febriani Emyliana Listiowati, Emyliana Eric Armando Fani Fariedah Faqih, Abd. Raheem Faqih, Abdul Raheem Fariedah, Fani Fitri Sil Valen Frits Jamlaay, Frits Handoko, Lugu Tri Harahap, Muhammad Alfiandi Rachmad Hariati , Anik Martinah Ihsan, Gusti Abdillah Ir. Mulyanto, MS Ir. Mulyanto, MS, Ir. Mulyanto, Irfanov Hafiz Kholil, Kiki Nur Azam Kurniasari, Reni Dyah Yuni Kusuma, Baruna Kusuma, Bayu Kusuma, Wahyu Endra Lestari, Bela Fatma Hani Ayu Lugu Tri Handoko Lugu Tri Handoko Handoko Lutviana N.D Marsoedi Marsoedi Marsoedi Marsoedi Maulana, Antajala Rian Mayor, Corazon Ell Merilia Papuani Milansari, Sindi Mimit Primyastanto Mimit Primyastanto Mimit Primyastanto Mohamad Fadjar Muh. Sulaiman Dadiono Muhammad Dailami, Muhammad Muhammad Fadjar Mujtahidah, Tholibah Nadia Dara Panggita Wati Nadiro, Vina Nur Niken Hendrakusma Wardani Nurcahyani, Shinta Eka Nurhalisa Nurhalisa Paricahya, Akhsan Fikrillah Pratama, Rakhmad Ndaru Putri, Radwa Fathin H R Adharyan Islamy Rahayu, Andra Rejekining Ramadhan, Surya Dewa Risnita Tri Utami Utami Rizal Akbar Hutagalung, Rizal Akbar Rohmah Widiastuti Rudianto Samsudin Hariyanto Saputra Fredo Seftiawan Samsu Rijal Septiana Sri Astuti, Septiana Sri Siahaan, Marthin Daniel Theo Gratia Sinaga, Sorbakti Soemarno Soemarno Soko Nuswantoro Sri Andayani Sri Andayani Sudjatmiko, Sudjatmiko Sufaichusan, Ifa Sugestya, I Nengah Gde Suharun Martudi Supriatna Taufik Budhi Pramono Taufik Budhi Pramono Tholibah Mujtahidah tholibah mujtahidah Veryl Hasan Vina Nur Nadiro Wahida Kartika Sari Wati, Nadia Dara Panggita Wijaya, Harun Wijaya, Rudy Yanuhar, Uun Yuni Kilawati Yunita Maimunah