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Effect of Dosages and Temperatures on Simalungun Red Ginger (Zingiber officinale var. Rubrum) Ethanol Extract Dipping on Masculinization of Zebra Fish (Danio rerio) as a Model Fish Eka Fitria Febriani; Maheno Sri Widodo; Abdul Rahem Faqih
Jurnal Ilmiah Perikanan dan Kelautan Vol. 15 No. 1 (2023): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v15i1.38271

Abstract

Highlight Research Successive administration of Ginger ethanol extract to male individuals was shown to be able to produce a significant increase in sperm count, motility, viability and morphology Ethanol extract of ginger has androgenic activity which is proven to be able to increase the concentration of testosterone in serum, which functions to control the process of spermatogenesis, maintain Sertoli cells and play a role in determining the quality of spermatozoa in male organisms Giving ginger ethanol extract for 30 consecutive days to male individuals showed significant results so that ginger extract was thought to have the potential to improve male characteristics Ginger extract is potentially useful in improving healthy sperm characteristics and management of male infertility especially in those with low sperm counts   Abstract Ornamental fish are known to be easily cultivated in aquarium or pond. Many people prefer male (MF) over female fish (FF) due to their aesthetic value and productivity which lead to better pricing. The aim of this study was to determine the effect of Simalungun Red Ginger (Zingiber officinale var. Rubrum) (SRG) on masculinization. This study used dipping method which was carried out using Simalungun Red Ginger (Zingiber officinale var. Rubrum) (SRG). The temperature treatments used in this study were 28°C and 32°C. For this research, an experimental study with a Completely Randomized Factorial Design (CRFD) method that had two independent variables such as different dosages and temperatures was used. From five dosages (0 mg/L; 5 mg/L; 10 mg/L; 15 mg/L; 20 mg/L) and three temperature treatments (ambient temperature (about 25°C; 28°C; 32°C), it was found that the highest male percentage of primary sexuality (88.33%), secondary sexuality (85%), and highest total testosterone levels (TL) (1.986 ng/L) were at the treatments with the highest dosages and temperature, while for the highest survival rate were at almost in all B treatments (5 mg/L) and b levels (28°C), in which the survival rate was 100%. For all of these results above, the results were significant.
Sustainable Pangasius Aquaculture Management Strategy using Multidimensional Scaling (MDS) and Analytical Hierarchy Process (AHP) in Tulungagung Regency, East Java, Indonesia Asro Nurhabib; Aida Sartimbul; Mimit Primyastanto; Maheno Sri Widodo; Lugu Tri Handoko; Andra Rejekining Rahayu; Suharun Martudi
Jurnal Ilmiah Perikanan dan Kelautan Vol. 16 No. 1 (2024): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v16i1.49377

Abstract

Graphical Abstract   Highlight Research The author mentioned four highlights from their research: Tulungagung Regency is East Java Province's major pangasius producer. MDS can assess sustainability across multiple dimensions. AHP enables the prioritization of strategies that must be implemented. The Pangasius aquaculture company in Tulungagung Regency is currently in a sustainable state. Abstract Aquaculture activities in Tulungagung Regency provide livelihoods for 12,050 households. Therefore, ensuring the sustainability of Pangasius aquaculture activities is crucial for the welfare of fish farmers. This study aims to analyze the sustainability of existing Pangasius aquaculture businesses in Tulungagung Regency, East Java, Indonesia, and to create a sustainable Pangasius aquaculture policy strategy. This quantitative study used Multi-Dimensional Scaling (MDS) and Analytical Hierarchy Process (AHP) analyses to determine the sustainability status of Pangasius aquaculture based on five dimensions: ecological, economic, social, institutional, and technological infrastructure. Among these dimensions, two are less sustainable, particularly infrastructure technology and the economy.
Pendugaan Fekunditas Induk Betina Ikan Nilem (Osteochilus vittatus) berdasarkan Karakter Morfometrik Ramadhan, Surya Dewa; Widodo, Maheno Sri; Ariyani, Destia Fitri; Pratama, Rakhmad Ndaru; Dailami, Muhammad
ACROPORA: Jurnal Ilmu Kelautan dan Perikanan Papua Vol 7 No 2 (2024): ACROPORA: Jurnal Ilmu Kelautan dan Perikanan Papua
Publisher : Cenderawasih University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31957/acr.v7i2.4389

Abstract

Ikan nilem merupakan ikan air tawar asli Indonesia yang persebaranya hanya di daerah Jawa Barat. Induk betina ikan nilem memiliki jumlah telur yang bervariasi sehingga perlu dilakukan seleksi. Seleksi yang dapat dilakukan pada induk betina ikan nilem yaitu dengan cara melihat karakter morfometrik. Sampai saat ini penelitian tentang ikan nilem khususnya yang berkaitan dengan reproduksi juga masih sangat jarang dilakukan. Tujuan dilaksanakanya penelitian ini adalah untuk menganalisis hubungan dan mengetahui karakter morfometrik yang paling berhubungan terhadap fekunditas. Metode yang digunakan dalam penelitian iniyaitu deskripstif komparatif dengan pendekatan kuantitatif. Parameter yang diamati meliputi panjang standar, tinggi badan, lingkar tubuh, dan bobot tubuh. Kelompok sampel ikan uji diukur berdasarkan panjang standar yang telah ditentukan, yaitu 06,1-09 cm, 09,1-12 cm, 12,1-15 cm, 15,1- 18 cm, 18,1-21 cm. Hasil penelitian menunjukkan bahwa morfometrik berpengaruh terhadap fekunditas. Karakter morfometrik terhadap fekunditas diperoleh hasil tertinggi pada lingkar tubuh dengan fekunditas sebesar 99%terhadap fekunditas yang dihasilkan pada induk betina ikan nilem. Faktor yang dapat mempengaruhi jumlah fekunditas diantaranya yaitu umur ikan, lingkungan, makanan, dan diameter ikan. Diameter ikan sangat berpengaruh terhadap jumlah fekunditas yang di hasilkan, semakin besar diameter telur dalam suatu gonad maka akan semakin sedikit jumlah telur yang dihasilkan.
Evaluation of PMSG (Oodev®) application on hepatosomatic and gonadosomatic index of snakehead fish Hutagalung, Rizal Akbar; Widodo, Maheno Sri; Faqih, Abdul Rahem
Jurnal Akuakultur Indonesia Vol. 14 No. 1 (2015): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2996.134 KB) | DOI: 10.19027/jai.14.24-29

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ABSTRACT Snakehead fish (Channa striata) is a freshwater fish commodities that have high economic value, while their needs depend on natural catching. In general, hormonal induction commonly used for the efficiency of female parent utilization and enhancement quality and quantity of fish fry. One of the hormones that can be used is pregnant mare serum gonadotropin (PMSG) and antidopamine with trademark Oodev®. These hormones contain many elements follicle stimulating hormone (FSH) which can trigger the early stages of gonad maturity. The purpose of this research was to determine the effectiveness of hormone PMSG on gonadosomatic index (GSI) and hepatosomatic index (HSI). The size of female fish was 30−40 cm, weighing of 500−700 g, adapted in the aquarium and then induced with different doses of PMSG, namely: treatment A dose: 0.75 mL/kg; B: 1.0 mL/kg; C: 1.25 mL/kg; D: 1.5 mL/kg and be repeated three times. Fishes were then reared up to 72 hours to determine the development of GSI and HSI. The results showed that the best treatment was at dose of 1.25 mL/kg with GSI and HSI values of 1.37% and 3.35%, respectively. Keywords: PMSG, GSI, HSI, snakehead fish  ABSTRAK Ikan gabus (Channa striata) merupakan salah satu komoditas air tawar yang mempunyai nilai ekonomi tinggi. Saat ini pemenuhan kebutuhan hanya bergantung pada hasil penangkapan dari alam. Pada umumnya cara pemijahan buatan dengan induksi hormon dilakukan untuk efisiensi penggunaan induk serta peningkatan kualitas dan kuantitas benih ikan. Salah satu hormon yang dapat digunakan adalah pregnant mare serum gonadotropin (PMSG) dan antidopamin dengan merk dagang Oodev®. Hormon-hormon tersebut banyak mengandung unsur follicle stimulating hormon (FSH) yang dapat memicu kematangan gonad tahap awal. Tujuan dari penelitian ini adalah untuk menguji efektivitas hormon PMSG terhadap nilai indeks gonadosomatik (IGS) dan indeks hepatosomatik (IHS). Induk ikan gabus yang digunakan berukuran 30−40 cm dengan bobot 500–700 g, diaklimatisasikan di akuarium kemudian diinduksi hormon PMSG dengan perlakuan dosis A: 0,75 mL/kg; B: 1,0 mL/kg; C: 1,25 mL/kg; D: 1,5 mL/kg dan dilakukan pengulangan sebanyak tiga kali. Selanjutnya ditunggu hingga 72 jam untuk mengevaluasi perkembangan IGS dan IHS nya. Hasil penelitian menunjukkan bahwa perlakuan terbaik adalah dosis 1,25 ml/kg dengan nilai rata-rata IHS 1,37% dan IGS 3,35%, Kata kunci: PMSG, IGS, IHS, ikan gabus 
Ovulation time and ovulated eggs count of sneakehead Channa striata induced by prostaglandin 2α with different doses Jamlaay, Frits; Widodo, Maheno Sri; Faqih, Abd. Rahem
Jurnal Akuakultur Indonesia Vol. 15 No. 1 (2016): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3067.389 KB) | DOI: 10.19027/jai.15.89-92

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ABSTRACT Snakehead fish Channa striata is one of freshwater commodity which has high economic value. Nowadays, snakehead fish stock mostly comes from wild capture. Artificial spawning with hormonal induction is usually used to improve broodstock efficiency, as well as the quality and quantity of fish seed production. Purpose of this study was to determine the effectiveness of prostaglandin 2α (PGF2α) hormone on ovulation time and count of ovulated eggs. This experiment used 30–40 cm broodstock with 600–900 g body weight. Fishes were acclimated for one week in a container and then injected with different doses of PGF2α, namely: control without PGF2α (P1), 0.5 mL/kg (P2); 0.7mL/kg (P3); and 0.9 mL/kg (P4). Each treatment consisted of three fishes as replication. Ovulation time was observed until 72 hours post injection. The results showed that PGF2α injected broodstoks ovulated faster (9.17–12.24 hours post injection) than control P1 (22.67 hours), while among PGF2α injected fishes were the same. Furthermore, count of ovulated eggs from PGF2α induced broodstocks were higher than control P1, and the highest (2,860 eggs) was obtained in treatment 0.9 mL/kg. Thus, PGF2α at dose of 0.9 mL/kg can be used to induce eggs ovulation of snakehead fish and to increase count of ovulated eggs. Keywords: PGF2α, ovulation time, ovulated eggs count, Channa striata  ABSTRAK Ikan gabus Channa striata merupakan salah satu komoditas air tawar yang mempunyai nilai ekonomi tinggi. Saat ini, pemenuhan kebutuhan hanya bergantung pada hasil penangkapan di alam. Cara pemijahan buatan dengan induksi hormon biasanya digunakan untuk efisiensi penggunaan induk serta peningkatan kualitas dan kuantitas benih ikan yang dihasilkan. Tujuan dari penelitian ini adalah untuk menguji efektivitas hormon prostaglandin 2α (PGF2α) terhadap waktu ovulasi dan jumlah telur ikan gabus yang diovulasikan. Penelitian ini menggunakan induk ikan gabus berukuran 30–40 cm dengan bobot tubuh 600–900 g. Ikan diadaptasikan di wadah penelitian selama seminggu, kemudian diinjeksi hormon PGF2α dengan perlakuan hormon dengan dosis berbeda, yaitu: kontrol tanpa hormon (P1), 0,5 mL/kg (P2); 0,7 mL/kg (P3); dan 0,9 mL/kg (P4). Setiap perlakuan terdiri atas tiga ekor induk sebagai ulangan. Waktu ovulasi diamati sampai dengan 72 jam pascasuntik. Hasil penelitian menunjukkan bahwa induk yang disuntik PGF2α memijah lebih cepat (9,17–12,24 jam pascasuntik) daripada kontrol (22,67 jam), sedangkan antarperlakuan hormon PGF2α tidak berbeda. Selanjutnya, jumlah telur diovulasi dari induk ikan gabus yang disuntik PGF2α berjumlah lebih banyak daripada kontrol. Hasil tertinggi (2.860 butir) diperoleh dari perlakuan 0,9 mL/kg.  Dengan demikian, hormon PGF2α dosis 0,9 mL/kg dapat digunakan untuk mempercepat waktu ovulasi ikan gabus, dan meningkatkan jumlah telur yang diovulasikan. Kata kunci: PGF2α, waktu ovulasi, jumlah telur diovulasi, Channa striata 
Analysis of Substrate, Gastric Content and Nutrient Content of Varuna litterata Sorbakti Sinaga; Maheno Sri Widodo; Yunita Maimunah
Jurnal Penelitian Pendidikan IPA Vol 10 No 11 (2024): November
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v10i11.7691

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Varuna litterata is a secondary freshwater crab found in tidal areas. This crab is caught, its eggs are taken, and it is considered a delicacy. Continuous fishing without any cultivation efforts can cause a decline in the population, leading to extinction. This study aims to determine the characteristics of the habitat substrate, stomach, and nutritional content of V. litterata. The method used in this study is descriptive exploratory. The study was conducted in the Serayu River for four months (October 2023 - January 2024). The observation location was carried out at three stations. The study showed that V. litterata prefers waters with clay sand substrates (75% sand, 25% dust, and 0% clay). This habitat is very suitable for cultivation development, as shown by the population of captured crabs. The results of observations of stomach contents show that this crab is an opportunistic omnivore. This crab has a high nutritional content with an average water content (60.72%), ash (4.57%), fat (0.52%), protein (25.66%), and carbohydrates (8.52%). Further research is essential to understand the reproductive system of V. litterata, its life cycle, and suitable habitats for spawning.
Analisis Morfologi Integratif Ikan Berod (Mastacembelus unicolor) di Sungai Brantas, Jawa Timur Ifa Sufaichusan; Septiana Sri Astuti; Akhsan Fikrillah Paricahya; Bela Fatma Hani Ayu Lestari; Kiki Nur Azam Kholil; Fisma Josara Apriliyanti; Ating Yuniarti; Maheno Sri Widodo; Wahyu Endra Kusuma
Jurnal Penelitian Pendidikan IPA Vol 10 No 11 (2024): November
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v10i11.7978

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Mastacembelus sp., a member of the Mastacembelidae family, is distributed across Java, Sumatera, and Kalimantan. Mastacembelus, locally named berod fish in the Brantas River lacks population’s descriptions and morphological analyses. Morphological analyses, including morphometric and meristic descriptions, are important for precise identification and classification of kinship relationships, serving as a cornerstone in the fields of biology, taxonomy, and conservation. This study aimed to conduct a comprehensive morphometric and meristic identification of the berod fish population in the Brantas River, East Java. A total of 37 samples were collected from 6 different locations: Blitar, Tulungagung, Kediri1, Kediri2, Nganjuk, and Mojokerto. The morphometric measurements were based on 25 characters, while the meristic calculations were based on 6 characters. The data analyzed with PCA and CA using SPSS and PAST programs. The results revealed that berod fish in the Brantas River had morphological characters belongs to the Mastacembelus unicolor. However, notable disparities were observed in meristic characters, particularly in the Dorsal Fin Spines (DFS). PCA analysis showed significant differences in morphometric characters but not supported by meristic characters. These results underscore the importance of integration both morphological and meristic analyses for a comprehensive understanding of the berod fish population.
The Impact of Artificial Barriers on the Varuna litterata Migration Route in the Lower Serayu River, Central Java and Its Molecular Identification Sinaga, Sorbakti; Maimunah, Yunita; Ekawati, Arning Wilujeng; Widodo, Maheno Sri
Journal Omni-Akuatika Vol 20, No 1 (2024): Omni-Akuatika May
Publisher : Fisheries and Marine Science Faculty - Jenderal Soedirman University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.oa.2024.20.1.1140

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The Serayu River is one of the longest rivers in Central Java. Dams built in the lower reaches of the Serayu River are thought to block the migration paths of fish and aquatic macrocrustaceans. This study aimed to determine the effect of dam on Varuna litterata migration and its molecular identification. This research was conducted using the observation method. Migration observations were made on vertical paths while molecular identification of V. litterata in the lower reaches of the Serayu River was compared with existing data in GenBank. The study covered the freshwater area above the Serayu Dam, the open area with freshwater below the Serayu Dam, and the open area with brackish water at the estuary of the Serayu River, with a study length of 33.37 km. The results showed that the presence of dams in the lower reaches of the Serayu River inhibited the migration pattern of V. litterata. The vertical shape of the dam causes only a few V. litterata to migrate to the top of the dam. The dam in the lower reaches of the Serayu River resulted in not many V. litterata being able to migrate to the top of the dam, so there were very few V. litterata found in the population upstream of the dam (115 ind). The results of molecular identification of V. litratata using the cytochrome C oxidase subunit I (COI) gene show that this crab is 100% the same as the data in GenBank and is closely related to Varuna yui and other Varunidae crab species.Keywords: Dams, Molecular, Migration, Serayu River, Varuna litterata
Reproductive Hormone Performance: Glass Strain Common carp (Cyprinus carpio) Reared in The Wet and Dry Seasons Nadia Dara Panggita Wati; Maheno Sri Widodo; Anik Martinah Hariati
International Journal of Marine Engineering Innovation and Research Vol 9, No 4 (2024)
Publisher : Institut Teknologi Sepuluh Nopember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12962/j25481479.v9i4.22120

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Glass strain carp (Cyprinus carpio) is highly demanded by farmers due to its delicious, dense meat and fewer spines. To meet the increasing demand for carp, it is necessary to improve the efficiency of aquaculture. The season is known to stimulate one hormone production. The purpose of this study was to determine the effect of season (rainy and dry seasons) on reproductive hormones (follicle stimulating hormone (FSH), luteinizing hormone (LH), testosterone and estrogen, and interstitial cell-stimulating hormone)) in C. carpio glass strain. Idi research was conducted using the descriptive method by rearing fish in different seasons (rainy season and dry season). The results showed that the reproductive hormone of C. carpio strain glass increased during the rainy season. During the rainy season, changes in the environment and water flow patterns are detected by the fish's sensory system. The results showed that the highest hormones in a row were: ICSH hormone (12.82 pg/mL), FSH hormone (male: 3.73 ng/mL; female: 12.10 ng/mL), LH hormone (12.48 MIU/ml), testosterone hormone (9.27 ng/mL) and estrogen hormone (21.84 ng/mL). Further research is needed to observe the growth and abnormality of fish larvae produced in different seasons.
Sperm Quality and Fecundity of Glass Carp (Cyprinus carpio) Strain Produced in the Rainy and Dry Seasons Nadia Dara Panggita Wati; Maheno Sri Widodo; Anik Martinah Hariati
Jurnal Penelitian Pendidikan IPA Vol 11 No 1 (2025): January
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v11i1.9791

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Carp (Cyprinus carpio) is one of the most widely cultivated freshwater fish species worldwide. C. carpio is increasingly widespread with the presence of the C. carpio glass strain. This fish farming is inseparable from the influence of the season. The purpose of this study was to determine the sperm quality and fertility of glass carp strain produced in the rainy season and dry season. The method used in this research is descriptive. This research was conducted for 3 months (April - June 2024). Samples of glass strain carp C. carpio broodstock and research carried out at the Punten Aquaculture Fisheries Installation (IPB), with a total of 12 broodstock (6 males and 6 females with body size between 25-30 cm). The results showed that the sperm of C. carpio glass strain fish produced in different seasons showed different quality. The best sperm quality was produced in the rainy season with motility (70.44%), concentration (26.75 X 109 cells/ml), and viability (79.81%). However, the rainy season produced the highest abnormality (5.53%). Fecundity of C. carpio glass strain fish was highest in the rainy season reaching 10,875 grains. Further research is needed to determine the success of carp larvae in different seasons.
Co-Authors Abdul Raheem Faqih Abdul Rahem Faqih Agoes Soeprijanto, Agoes Aida Sartimbul Aida Sartimbul Akhsan Fikrillah Paricahya Amalia Ayuk Riyadini Amalia, Elok Andi Masriah Andik Isdianto Andra Rejekineng Rahayu Andra Rejekineng Rahayu Rahayu Andra Rejekining Rahayu Angga Wira Perdana Anik Martinah Hariati Ariyani, Destia Fitri Arning Wilujeng Ekawati Ating Yuniarti Bayu Kusuma Bayu Kusuma Bela Fatma Hani Ayu Lestari Dedi Pardiansyah Dewa Gede Raka Wiadnya Dewi Susylowati Diana Arfiati Eka Fitria Febriani Emyliana Listiowati, Emyliana Eric Armando Fani Fariedah Fariedah, Fani Fisma Josara Apriliyanti Fitri Sil Valen Frits Jamlaay, Frits Harahap, Muhammad Alfiandi Rachmad Hariati, Anik Martinah Harsuko Riniwati Harun Wijaya I Nengah Gde Sugestya Ifa Sufaichusan Ir. Mulyanto, MS Ir. Mulyanto, MS, Ir. Mulyanto, Irfanov Hafiz Kiki Nur Azam Kholil Kurniasari, Reni Dyah Yuni Kusuma, Baruna Kusuma, Bayu Kusuma, Wahyu Endra Lugu Tri Handoko Lugu Tri Handoko Handoko Lutviana N.D Marsoedi Marsoedi Marsoedi Marsoedi Mimit Primyastanto Mimit Primyastanto Mohamad Fadjar Muh. Sulaiman Dadiono Muhammad Azmi Amanullah Muhammad Dailami, Muhammad Muhammad Fadjar Mujtahidah, Tholibah Nadia Dara Panggita Wati Nadia Dara Panggita Wati Nadiro, Vina Nur Niken Hendrakusma Wardani Nurhabib, Asro Nurhalisa Nurhalisa Pratama, Rakhmad Ndaru R Adharyan Islamy Ramadhan, Surya Dewa Risnita Tri Utami Risnita Tri Utami Utami Rizal Akbar Hutagalung, Rizal Akbar Rohmah Widiastuti Rudianto Rudy Wijaya Samsudin Hariyanto Saputra Fredo Seftiawan Samsu Rijal Septiana Sri Astuti Sinaga, Sorbakti Soemarno Soemarno Soko Nuswantoro Sorbakti Sinaga Sri Andayani Sri Andayani Sudjatmiko, Sudjatmiko Sufaichusan, Ifa Suharun Martudi Supriatna Tatang Tatang Taufik Budhi Pramono Taufik Budhi Pramono tholibah mujtahidah Tholibah Mujtahidah Veryl Hasan Vina Nur Nadiro Wahida Kartika Sari Wahyu Endra Kusuma Yanuhar, Uun Yuni Kilawati Yunita Maimunah