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All Journal Jurnal Akuakultur Indonesia Jurnal Dedikasi BIOTROPIA - The Southeast Asian Journal of Tropical Biology The Journal of Experimental Life Sciences (JELS) Journal of Innovation and Applied Technology Research Journal of Life Science Journal of Environmental Engineering and Sustainable Technology Jurnal Sumberdaya Akuatik Indopasifik JFMR (Journal of Fisheries and Marine Research) Omni-Akuatika Jurnal Penelitian Pendidikan IPA (JPPIPA) Samakia: Jurnal Ilmu Perikanan Journal of Aquaculture and Fish Health Syntax Idea Indonesian Journal of Tropical Aquatic Abdimas: Jurnal Pengabdian Masyarakat Universitas Merdeka Malang Journal Of Aquaculture Development and Environment Journal of Applied Science, Engineering, Technology, and Education International Journal of Marine Engineering Innovation and Research Proceedings Series on Physical & Formal Sciences ACROPORA: Jurnal Ilmu Kelautan dan Perikanan Papua International Journal of Sustainable Competitiveness on Tourism Journal of Fisheries & Marine Jurnal Perikanan Journal of Tropical Diversity
Maheno Sri Widodo, Maheno Sri
Program Studi Budidaya Perairan - Fakultas Perikanan Dan Ilmu Kelautan Universitas Brawijaya
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The Effect of Difference Temperature on Cortisol, Glucose and Glycogen level of Uceng Fish (Nemacheilus fasciatus) Wijaya, Harun; Widodo, Maheno Sri; Soeprijanto, Agoes
The Journal of Experimental Life Science Vol. 9 No. 1 (2019)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (557.651 KB) | DOI: 10.21776/ub.jels.2019.009.01.01

Abstract

Nemacheilus is one of the genera of freshwater fish. The existence of Uceng fish (Nemacheilus fasciatus) in public waters is increasingly rare. Changes in water temperature in the maintenance medium will affect the physiological processes of fish. The purpose of this study was to analyze physiological responses (blood glucose levels, cortisol, and glycogen hormones) of Uceng Fish (N. fasciatus) which were incubated at different temperatures and find out the initial time of Uceng fish adaptation to its new environment. Total of 200 fish were collected from wild catches in the Lekso River, Blitar. Fish tissue collection was carried out to test the profile of glucose, cortisol, and glycogen. The research activities at this stage were carried out by raising the Uceng fish from nature to a cultivation container in an aquarium of size 50 x 30 x 30 cm that given the different temperatures (20°C, 24°C, 28°C and 32°C), and each aquarium is filled with 10 Uceng fish and will be kept for 14 days. ELISA method was used to quantify the parameter. The result of this study showed highest peak of cortisol levels and blood glucose levels was achieved in the treatment of 24°C on the 10th day and the lowest in the treatment temperature of 28°C on the 6th day. The highest glycogen peak was reached at 32°C on day 10 and the lowest peak was reached at 28°C on day 2. We conclude that temperature affects the physiological response (cortisol, blood glucose, and glycogen) of Uceng fish (N. fasciatus). High levels of cortisol and blood glucose indicate stressful fish. Keywords: Fish, Nemacheilus fasciatus, Physiological, Temperature, Uceng.
Molecular Identification and Genetic Characteristics of Genus Mystacoleucus Based on Gene Cytochrome Oxidase C Subunit I (COI) in Sengguruh Dam Valen, Fitri Sil; Widodo, Maheno Sri; Kilawati, Yuni
The Journal of Experimental Life Science Vol. 9 No. 1 (2019)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1080.646 KB) | DOI: 10.21776/ub.jels.2019.009.01.07

Abstract

In general, the process of naming several species of fish including species from the genus Mystacoleucus is still carried out based on morphological characters, which are often found in almost the same morphological characters in different fish species. Therefore, it is necessary to apply a more accurate identification method, which is a DNA-based identification method called DNA barcoding. The purpose of this study was to identify the species of the genus Mystacoleucus on the Sengguruh Dam molecularly based on the Cytochrome Oxidase C Subunit I (COI) gene. A caudal fin was taken on the test fish and preserved in 95% ethanol solution for molecular identification. The results of the identification showed that the sample belonged to the Mystacoleucus marginatus species with Identity values between 99-100% and E-value 0.0. The data obtained showed that from the calculation of genetic distance presented in the form of data matrix and phylogenetic tree reconstruction, there were 2 species that had a far genetic distance from the M. marginatus sample from the Sengguruh Dam namely M. atridorsalis with the furthest genetic distance of 0.1932-0.2595 and M. lepturus with genetic distance between 0.1117-0.1193. However, there are one species that has the closest distance, M. padangensis with genetic distance between 0.0019-0.0038 and identity values up to 99%.Keywords: DNA barcode, Molecular identification, Mystacoleucus, Phylogenetic COI.
Analysis of Substrate, Gastric Content and Nutrient Content of Varuna litterata Sorbakti Sinaga; Maheno Sri Widodo; Yunita Maimunah
Jurnal Penelitian Pendidikan IPA Vol 10 No 11 (2024): November
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v10i11.7691

Abstract

Varuna litterata is a secondary freshwater crab found in tidal areas. This crab is caught, its eggs are taken, and it is considered a delicacy. Continuous fishing without any cultivation efforts can cause a decline in the population, leading to extinction. This study aims to determine the characteristics of the habitat substrate, stomach, and nutritional content of V. litterata. The method used in this study is descriptive exploratory. The study was conducted in the Serayu River for four months (October 2023 - January 2024). The observation location was carried out at three stations. The study showed that V. litterata prefers waters with clay sand substrates (75% sand, 25% dust, and 0% clay). This habitat is very suitable for cultivation development, as shown by the population of captured crabs. The results of observations of stomach contents show that this crab is an opportunistic omnivore. This crab has a high nutritional content with an average water content (60.72%), ash (4.57%), fat (0.52%), protein (25.66%), and carbohydrates (8.52%). Further research is essential to understand the reproductive system of V. litterata, its life cycle, and suitable habitats for spawning.
Analisis Morfologi Integratif Ikan Berod (Mastacembelus unicolor) di Sungai Brantas, Jawa Timur Ifa Sufaichusan; Septiana Sri Astuti; Akhsan Fikrillah Paricahya; Bela Fatma Hani Ayu Lestari; Kiki Nur Azam Kholil; Fisma Josara Apriliyanti; Ating Yuniarti; Maheno Sri Widodo; Wahyu Endra Kusuma
Jurnal Penelitian Pendidikan IPA Vol 10 No 11 (2024): November
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v10i11.7978

Abstract

Mastacembelus sp., a member of the Mastacembelidae family, is distributed across Java, Sumatera, and Kalimantan. Mastacembelus, locally named berod fish in the Brantas River lacks population’s descriptions and morphological analyses. Morphological analyses, including morphometric and meristic descriptions, are important for precise identification and classification of kinship relationships, serving as a cornerstone in the fields of biology, taxonomy, and conservation. This study aimed to conduct a comprehensive morphometric and meristic identification of the berod fish population in the Brantas River, East Java. A total of 37 samples were collected from 6 different locations: Blitar, Tulungagung, Kediri1, Kediri2, Nganjuk, and Mojokerto. The morphometric measurements were based on 25 characters, while the meristic calculations were based on 6 characters. The data analyzed with PCA and CA using SPSS and PAST programs. The results revealed that berod fish in the Brantas River had morphological characters belongs to the Mastacembelus unicolor. However, notable disparities were observed in meristic characters, particularly in the Dorsal Fin Spines (DFS). PCA analysis showed significant differences in morphometric characters but not supported by meristic characters. These results underscore the importance of integration both morphological and meristic analyses for a comprehensive understanding of the berod fish population.
Sperm Quality and Fecundity of Glass Carp (Cyprinus carpio) Strain Produced in the Rainy and Dry Seasons Nadia Dara Panggita Wati; Maheno Sri Widodo; Anik Martinah Hariati
Jurnal Penelitian Pendidikan IPA Vol 11 No 1 (2025): January
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v11i1.9791

Abstract

Carp (Cyprinus carpio) is one of the most widely cultivated freshwater fish species worldwide. C. carpio is increasingly widespread with the presence of the C. carpio glass strain. This fish farming is inseparable from the influence of the season. The purpose of this study was to determine the sperm quality and fertility of glass carp strain produced in the rainy season and dry season. The method used in this research is descriptive. This research was conducted for 3 months (April - June 2024). Samples of glass strain carp C. carpio broodstock and research carried out at the Punten Aquaculture Fisheries Installation (IPB), with a total of 12 broodstock (6 males and 6 females with body size between 25-30 cm). The results showed that the sperm of C. carpio glass strain fish produced in different seasons showed different quality. The best sperm quality was produced in the rainy season with motility (70.44%), concentration (26.75 X 109 cells/ml), and viability (79.81%). However, the rainy season produced the highest abnormality (5.53%). Fecundity of C. carpio glass strain fish was highest in the rainy season reaching 10,875 grains. Further research is needed to determine the success of carp larvae in different seasons.
Co-Authors Abdul Rahem Faqih Agoes Soeprijanto, Agoes Aida Sartimbul Aida Sartimbul Aida Sartimbul Akhsan Fikrillah Paricahya Amalia Ayuk Riyadini Amalia, Elok Amanullah, Muhammad Azmi Andi Masriah Andik Isdianto Andra Rejekineng Rahayu Rahayu Andra Rejekining Rahayu Angga Wira Perdana Anik Martinah Hariati Ariyani, Destia Fitri Arning Wilujeng Ekawati Ating Yuniarti Ating Yuniarti Bayu Kusuma Bayu Kusuma Bela Fatma Hani Ayu Lestari Dedi Pardiansyah Dewa Gede Raka Wiadnya Dewi Susylowati Diana Arfiati Eka Fitria Febriani Emyliana Listiowati, Emyliana Eric Armando Fani Fariedah Faqih, Abdul Raheem Fariedah, Fani Fisma Josara Apriliyanti Fitri Sil Valen Frits Jamlaay, Frits Handoko, Lugu Tri Harahap, Muhammad Alfiandi Rachmad Ifa Sufaichusan Ir. Mulyanto, MS Ir. Mulyanto, MS, Ir. Mulyanto, Irfanov Hafiz Kiki Nur Azam Kholil Kurniasari, Reni Dyah Yuni Kusuma, Baruna Kusuma, Bayu Kusuma, Wahyu Endra Lugu Tri Handoko Lugu Tri Handoko Handoko Lutviana N.D Marsoedi Marsoedi Marsoedi Marsoedi Maulana, Antajala Rian Mayor, Corazon Ell Merilia Papuani Milansari, Sindi Mimit Primyastanto Mimit Primyastanto Mimit Primyastanto Mohamad Fadjar Muh. Sulaiman Dadiono Muhammad Dailami, Muhammad Muhammad Fadjar Mujtahidah, Tholibah Nadia Dara Panggita Wati Nadiro, Vina Nur Niken Hendrakusma Wardani Nurcahyani, Shinta Eka Nurhalisa Nurhalisa Pratama, Rakhmad Ndaru Putri, Radwa Fathin H R Adharyan Islamy Rahayu, Andra Rejekining Ramadhan, Surya Dewa Risnita Tri Utami Utami Rizal Akbar Hutagalung, Rizal Akbar Rohmah Widiastuti Rudianto Samsudin Hariyanto Saputra Fredo Seftiawan Samsu Rijal Septiana Sri Astuti Siahaan, Marthin Daniel Theo Gratia Sinaga, Sorbakti Soemarno Soemarno Soko Nuswantoro Sorbakti Sinaga Sri Andayani Sri Andayani Sudjatmiko, Sudjatmiko Sufaichusan, Ifa Sugestya, I Nengah Gde Suharun Martudi Supriatna Taufik Budhi Pramono Taufik Budhi Pramono tholibah mujtahidah Tholibah Mujtahidah Veryl Hasan Vina Nur Nadiro Wahida Kartika Sari Wahyu Endra Kusuma Wijaya, Harun Wijaya, Rudy Yanuhar, Uun Yuni Kilawati Yunita Maimunah