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All Journal Jurnal Akuakultur Indonesia Jurnal Dedikasi BIOTROPIA - The Southeast Asian Journal of Tropical Biology The Journal of Experimental Life Sciences (JELS) Journal of Innovation and Applied Technology Research Journal of Life Science Journal of Environmental Engineering and Sustainable Technology Jurnal Sumberdaya Akuatik Indopasifik JFMR (Journal of Fisheries and Marine Research) Omni-Akuatika Jurnal Penelitian Pendidikan IPA (JPPIPA) Samakia: Jurnal Ilmu Perikanan Journal of Aquaculture and Fish Health Syntax Idea Indonesian Journal of Tropical Aquatic Abdimas: Jurnal Pengabdian Masyarakat Universitas Merdeka Malang Journal Of Aquaculture Development and Environment Journal of Applied Science, Engineering, Technology, and Education International Journal of Marine Engineering Innovation and Research Proceedings Series on Physical & Formal Sciences ACROPORA: Jurnal Ilmu Kelautan dan Perikanan Papua International Journal of Sustainable Competitiveness on Tourism Journal of Fisheries & Marine Jurnal Perikanan Journal of Tropical Diversity

Maheno Sri Widodo, Maheno Sri

Program Studi Budidaya Perairan - Fakultas Perikanan Dan Ilmu Kelautan Universitas Brawijaya

Author-ID : 2587813

Aerospace Engineering Agriculture, Biological Sciences & Forestry Humanities Automotive Engineering Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Earth & Planetary Sciences Education Electrical & Electronics Engineering Energy Engineering Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Mechanical Engineering Medicine & Pharmacology Neuroscience Physics Public Health Social Sciences Transportation Veterinary Other

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Reproductive Hormone Performance: Glass Strain Common carp (Cyprinus carpio) Reared in The Wet and Dry Seasons Nadia Dara Panggita Wati; Maheno Sri Widodo; Anik Martinah Hariati
International Journal of Marine Engineering Innovation and Research Vol. 9 No. 4 (2024)
Publisher : Department of Marine Engineering, Institut Teknologi Sepuluh Nopember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12962/j25481479.v9i4.4895

Glass strain carp (Cyprinus carpio) is highly demanded by farmers due to its delicious, dense meat and fewer spines. To meet the increasing demand for carp, it is necessary to improve the efficiency of aquaculture. The season is known to stimulate one hormone production. The purpose of this study was to determine the effect of season (rainy and dry seasons) on reproductive hormones (follicle stimulating hormone (FSH), luteinizing hormone (LH), testosterone and estrogen, and interstitial cell-stimulating hormone)) in C. carpio glass strain. Idi research was conducted using the descriptive method by rearing fish in different seasons (rainy season and dry season). The results showed that the reproductive hormone of C. carpio strain glass increased during the rainy season. During the rainy season, changes in the environment and water flow patterns are detected by the fish's sensory system. The results showed that the highest hormones in a row were: ICSH hormone (12.82 pg/mL), FSH hormone (male: 3.73 ng/mL; female: 12.10 ng/mL), LH hormone (12.48 MIU/ml), testosterone hormone (9.27 ng/mL) and estrogen hormone (21.84 ng/mL). Further research is needed to observe the growth and abnormality of fish larvae produced in different seasons.

Effect of 17β-Estradiol on Feminization, Growth Rate and Survival Rate of Pacific White Shrimp (Litopenaeus vannamei, Boone 1931) Postlarvae Sugestya, I Nengah Gde; Widodo, Maheno Sri; Soeprijanto, Agoes
The Journal of Experimental Life Science Vol. 8 No. 1 (2018)
Publisher : Graduate School, Universitas Brawijaya

This study was therefore aimed to determine the effect of different concentration of estrogen hormone, 17β-estradiol (E2) on feminization for the production of all female, growth rate and survival rate of Pacific white shrimp (Litopenaeus vannamei) postlarvae (PL). L. vannamei PL1 were stocked randomly at a density of 10 ind.L-1 into a 10 L of seawater for each experimental flask with three replicates each. PL1 was immersed in seawater containing 0.5, 1.0 and 1.5 mg.L-1 estrogen hormone, E2 for 4 hours. The experiment was continued for 30 days for larval rearing. At the termination of experiment, the specimens in each treatment groups were weighed and measured individually for their wet body weight (BW) and total length (TL) for the mean weight gain, length gain, and the specific growth rate (SGR) estimation. The mean sex ratio values of male to female postlarvae obtained from control till the highest E2 hormone concentration of 0, 0.5, 1.0 and 1.5 mg.L-1 were 1:1, 1:2, 1:9 and 0:1 respectively. The weight gain and length gain were 40605% and 606% for control, 46310% and 647% for 0.5 mg.L-1 concentration, 49310% and 663% for 1.0 mg.L-1 concentration and 45048% and 628% for 1.5 mg.L-1. The mean SGR BW and SGR TL for control till the highest E2 hormone concentration of 0, 0.5, 1.0 and 1.5 mg.L-1 were 20.00 and 6.51, 20.46 and 6.70, 20.67 and 6.77 and 20.37 and 6.62 respectively. The mean survival rate for E2 hormone concentration of 0, 0.5, 1.0 and 1.5 mg.L-1 were 52.67%, 54.33%, 54.67% and 52.33% respectively. The study shows that while the concentration of E2 hormone increased, the female sex ratio of L. vannamei PL also increased but the growth rate and survival rate decreased at concentration of 1.5 mg.L-1. Thus the optimum concentration of this hormone usage was 1.0 mg.L-1 immersed for 4 hours. Keywords: 17β-estradiol, feminization, growth rate, Litopenaeus vannamei, postlarvae, survival rate.

The Effect of Difference Temperature on Cortisol, Glucose and Glycogen level of Uceng Fish (Nemacheilus fasciatus) Wijaya, Harun; Widodo, Maheno Sri; Soeprijanto, Agoes
The Journal of Experimental Life Science Vol. 9 No. 1 (2019)
Publisher : Graduate School, Universitas Brawijaya

Nemacheilus is one of the genera of freshwater fish. The existence of Uceng fish (Nemacheilus fasciatus) in public waters is increasingly rare. Changes in water temperature in the maintenance medium will affect the physiological processes of fish. The purpose of this study was to analyze physiological responses (blood glucose levels, cortisol, and glycogen hormones) of Uceng Fish (N. fasciatus) which were incubated at different temperatures and find out the initial time of Uceng fish adaptation to its new environment. Total of 200 fish were collected from wild catches in the Lekso River, Blitar. Fish tissue collection was carried out to test the profile of glucose, cortisol, and glycogen. The research activities at this stage were carried out by raising the Uceng fish from nature to a cultivation container in an aquarium of size 50 x 30 x 30 cm that given the different temperatures (20°C, 24°C, 28°C and 32°C), and each aquarium is filled with 10 Uceng fish and will be kept for 14 days. ELISA method was used to quantify the parameter. The result of this study showed highest peak of cortisol levels and blood glucose levels was achieved in the treatment of 24°C on the 10th day and the lowest in the treatment temperature of 28°C on the 6th day. The highest glycogen peak was reached at 32°C on day 10 and the lowest peak was reached at 28°C on day 2. We conclude that temperature affects the physiological response (cortisol, blood glucose, and glycogen) of Uceng fish (N. fasciatus). High levels of cortisol and blood glucose indicate stressful fish. Keywords: Fish, Nemacheilus fasciatus, Physiological, Temperature, Uceng.

Molecular Identification and Genetic Characteristics of Genus Mystacoleucus Based on Gene Cytochrome Oxidase C Subunit I (COI) in Sengguruh Dam Valen, Fitri Sil; Widodo, Maheno Sri; Kilawati, Yuni
The Journal of Experimental Life Science Vol. 9 No. 1 (2019)
Publisher : Graduate School, Universitas Brawijaya

In general, the process of naming several species of fish including species from the genus Mystacoleucus is still carried out based on morphological characters, which are often found in almost the same morphological characters in different fish species. Therefore, it is necessary to apply a more accurate identification method, which is a DNA-based identification method called DNA barcoding. The purpose of this study was to identify the species of the genus Mystacoleucus on the Sengguruh Dam molecularly based on the Cytochrome Oxidase C Subunit I (COI) gene. A caudal fin was taken on the test fish and preserved in 95% ethanol solution for molecular identification. The results of the identification showed that the sample belonged to the Mystacoleucus marginatus species with Identity values between 99-100% and E-value 0.0. The data obtained showed that from the calculation of genetic distance presented in the form of data matrix and phylogenetic tree reconstruction, there were 2 species that had a far genetic distance from the M. marginatus sample from the Sengguruh Dam namely M. atridorsalis with the furthest genetic distance of 0.1932-0.2595 and M. lepturus with genetic distance between 0.1117-0.1193. However, there are one species that has the closest distance, M. padangensis with genetic distance between 0.0019-0.0038 and identity values up to 99%.Keywords: DNA barcode, Molecular identification, Mystacoleucus, Phylogenetic COI.

Sperm Quality and Fecundity of Glass Carp (Cyprinus carpio) Strain Produced in the Rainy and Dry Seasons Wati, Nadia Dara Panggita; Widodo, Maheno Sri; Hariati , Anik Martinah
Jurnal Penelitian Pendidikan IPA Vol 11 No 1 (2025): January
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v11i1.9791

Carp (Cyprinus carpio) is one of the most widely cultivated freshwater fish species worldwide. C. carpio is increasingly widespread with the presence of the C. carpio glass strain. This fish farming is inseparable from the influence of the season. The purpose of this study was to determine the sperm quality and fertility of glass carp strain produced in the rainy season and dry season. The method used in this research is descriptive. This research was conducted for 3 months (April - June 2024). Samples of glass strain carp C. carpio broodstock and research carried out at the Punten Aquaculture Fisheries Installation (IPB), with a total of 12 broodstock (6 males and 6 females with body size between 25-30 cm). The results showed that the sperm of C. carpio glass strain fish produced in different seasons showed different quality. The best sperm quality was produced in the rainy season with motility (70.44%), concentration (26.75 X 109 cells/ml), and viability (79.81%). However, the rainy season produced the highest abnormality (5.53%). Fecundity of C. carpio glass strain fish was highest in the rainy season reaching 10,875 grains. Further research is needed to determine the success of carp larvae in different seasons.

Co-Authors Abdul Rahem Faqih Agoes Soeprijanto, Agoes Aida Sartimbul Aida Sartimbul Aida Sartimbul Amalia Ayuk Riyadini Amalia, Elok Amanullah, Muhammad Azmi Andi Masriah Andik Isdianto Andra Rejekineng Rahayu Rahayu Andra Rejekining Rahayu Angga Wira Perdana Anik Martinah Hariati Apriliyanti, Fisma Josara Ariyani, Destia Fitri Arning Wilujeng Ekawati Ating Yuniarti Bayu Kusuma Bayu Kusuma Dedi Pardiansyah Dewa Gede Raka Wiadnya Dewi Susylowati Diana Arfiati Eka Fitria Febriani Emyliana Listiowati, Emyliana Eric Armando Fani Fariedah Faqih, Abd. Raheem Faqih, Abdul Raheem Fariedah, Fani Fitri Sil Valen Frits Jamlaay, Frits Handoko, Lugu Tri Harahap, Muhammad Alfiandi Rachmad Hariati , Anik Martinah Ihsan, Gusti Abdillah Ir. Mulyanto, MS Ir. Mulyanto, MS, Ir. Mulyanto, Irfanov Hafiz Kholil, Kiki Nur Azam Kurniasari, Reni Dyah Yuni Kusuma, Baruna Kusuma, Bayu Kusuma, Wahyu Endra Lestari, Bela Fatma Hani Ayu Lugu Tri Handoko Lugu Tri Handoko Handoko Lutviana N.D Marsoedi Marsoedi Marsoedi Marsoedi Maulana, Antajala Rian Mayor, Corazon Ell Merilia Papuani Milansari, Sindi Mimit Primyastanto Mimit Primyastanto Mimit Primyastanto Mohamad Fadjar Muh. Sulaiman Dadiono Muhammad Dailami, Muhammad Muhammad Fadjar Mujtahidah, Tholibah Nadia Dara Panggita Wati Nadiro, Vina Nur Niken Hendrakusma Wardani Nurcahyani, Shinta Eka Nurhalisa Nurhalisa Paricahya, Akhsan Fikrillah Pratama, Rakhmad Ndaru Putri, Radwa Fathin H R Adharyan Islamy Rahayu, Andra Rejekining Ramadhan, Surya Dewa Risnita Tri Utami Utami Rizal Akbar Hutagalung, Rizal Akbar Rohmah Widiastuti Rudianto Samsudin Hariyanto Saputra Fredo Seftiawan Samsu Rijal Septiana Sri Astuti, Septiana Sri Siahaan, Marthin Daniel Theo Gratia Sinaga, Sorbakti Soemarno Soemarno Soko Nuswantoro Sri Andayani Sri Andayani Sudjatmiko, Sudjatmiko Sufaichusan, Ifa Sugestya, I Nengah Gde Suharun Martudi Supriatna Taufik Budhi Pramono Taufik Budhi Pramono tholibah mujtahidah Tholibah Mujtahidah Veryl Hasan Vina Nur Nadiro Wahida Kartika Sari Wati, Nadia Dara Panggita Wijaya, Harun Wijaya, Rudy Yanuhar, Uun Yuni Kilawati Yunita Maimunah

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