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Journal : Journal of Fisheries

DNA Barcoding of Cardinalfish (Apogonidae) in Gilimanuk Bay, Bali, Indonesia Putra, I Nyoman Giri Putra; Indrawan, Gede Surya; Faiqoh, Elok
Jurnal Ilmiah Perikanan dan Kelautan Vol. 16 No. 1 (2024): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.vi.43336

Abstract

Abstract The Apogonidae is estimated to consist of nearly 300 fish species, most of which inhabit coral reef areas. The lack of distinctive body markings and overlapping species distribution makes species assignment challenging. Therefore, this study aimed to delineate species and establish barcoding reference databases of Apogonidae in Gilimanuk Bay (Bali, Indonesia) using the Cytochrome Oxidase I (COI) gene of the mitochondrial DNA. A total of 22 fish tissue samples were extracted with 10% Chelex solution. BLAST analysis was performed and genetic differentiation between species was calculated. The phylogenetic tree was constructed using the Maximum Likelihood method and tree visualization was generated using iTOL V5. The morphology and genetic identification results based on the mitochondrial COI gene revealed eight species of seven genera, and one species was new to GenBank online database. This study was the first-ever addition of COI sequence for Ostorhinchus hartzfeldii into the GenBank database. The average K2P genetic distance within species and K2P distance between genera within the family were 0.60% and 19.10%, respectively. The mean genetic distance between genera within the family was 31.8-fold higher than the mean genetic distance within species. The phylogenetic tree showed that each sample resided in a distinct cluster, which indicates that DNA barcoding is a reliable and effective approach for species delimitation in Apogonidae fishes. Highlight Research Eight species of cardinalfish in Gilimanuk Bay were delineated using the barcoding method. This study provides the first nucleotide sequence for Ostorhinchus hartzfeldii in GenBank online database. The phylogenetic tree showed that each sample resided in a distinct cluster, indicating that the barcoding method efficiently differentiated at the species level. The mean genetic distance between genera within the family was 31.8-fold higher than the mean genetic distance within species.
Co-Authors A. A. Md. Ananda Putra Suardana Abd. Rahman As-syakur Abda Abda Agus Alfiyan Nur Ahmada, Muhamad Agustien Lilawati Agustina, Siti Rodliyah Eka Aji Wahyu Anggoro Al Umairi, Mushab Ali Mashar Allamah, Labibah Alvina Rahmayanti Amalia, Dwi Risky Anak Agung Istri Candra Kirana Andri Octapianus Purba Andrianus Sembiring Andrianus Sembiring Anggita Della Lestari Anisa Setyawati Ari Pratama, Yoga Ariandini, Yovita Aulia Dharma Nusa Pangestu Ayu, Inna Puspa Beginer Subhan Binti Nur Asiyah Budi Santoso Carolina Asrin Cornelia Coraima Lazaren Dede Nurohman Denisa, Ayu Dewa Ayu Wedha Astiti Dwi Budi Wiyanto Dwi Budi Wiyanto Enex Yuniarti Ningsih Erinda, Adysa Febriansyah, Fandi Febriyanto Arifin ferdi ardiansyah, ferdi Frida Purwanti Gede Surya Indrawan Hanifa Miranda I.H. Putri Hardianti Hardianti, Hardianti Hariyanto, Ropik Henny Hayati Heru Wicaksono I Dewa Nym. Nurweda P., I Gede Budi Astrawan I Gede Budi Astrawan I Gede Hendrawan I Gusti Ayu Dian Indraswari I Gusti Ayu Ricca Mahatma Putri I Gusti Bagus Sila Dharma I Gusti Bagus Sila Dharma I Gusti Ngurah Agung Dhananjaya I Gusti Ngurah Putra Dirgayusa I Kadek Dedy Antara Putra I Ketut Budiarta I Ketut Dedi Ariawan I Ketut Suada I Made Raditya Putra I Putu Irpan Aditanaya I Putu Sugiana I Putu Sugiana I Wayan Arthana I Wayan Eka Dharmawan I Wayan Eka Dharmawan I Wayan Gede Astawa Karang I Wayan Juliantara Putra IDA AYU ASTARINI Ida Bagus Indra Danendra Ifadah, Ayunda Sayyidatul Ikha Listyarini Indana Hilyatun Nafisah Indri Astutik Isaroh, Nila Latifatul Istri Pradnyandari Dewi, I Gusti Ayu Jatmiko, Dadang Heru Kadek Weda Ari Bhawana Kadek Yudiastuti Kisyanto, Edy Komang Indah Vitri Lestari Listyarini, lkha Lucia Hendrati, Lucia Luh Putu Puspita Dewanti Lutfinisa, Nur Aini Made Ayu Pratiwi Mashudi Mashudi Maulidia, Nur Fariha Mohammad Mukhlis Kamal Mubarika, Melinda Putri Muhammad Danie Al Malik Munawir Munawir Munawir Mushab Al Umairi Nabila, Putri Sukma Ngurah Ayu Nyoman Murniati Ni Luh Astria Yusmalinda Ni Luh Gede Widya Bintang Gustavina Ni Made Dewi Damayanti Ni Putu Ayu Aryanti Ni Putu Eva Damayanti Ni Wayan Loviasari Noorrahman, Handy Triya Pande Ditha Prasatia Pratama, Yoga Ari Prissanty, Anne Aninditha Putra, Alexander Vito Dharma Putra, I Nyoman Giri Putra, I Nyoman Giri Putra Putu Dian Pertiwi Putu Eva Setiawati Putu Satya Pratama Qomarul Huda, Qomarul Raharja, Mediana Emilia Raharjo, Totok Dwi Ranny R. Yuneni Ratih Permitha Syury Rizky Ramadhan Rohmah, Siti Lailatur Samantha Julia Blandina Lumban Tobing Sansabhilla, Faradiva Septi Novita Sari Siti Fatimah Siti Rudiyanti Siti Thomas Zulaikhah Susilawati, Susilawati Suyoto Suyoto Syam, Ferawaty Umar, Nugrah Juniar Vita Paramita Wahyudi, Pamungkas Tri Widiastuti Widiastuti Widya Rizky Pratiwi, Widya Rizky Wiratama, I Gusti Ngurah Made Yaniawati, Poppy Yaqin, Achmad Haqqul Yuliana Fitri Syamsuni Yuliastri, Windriyani Yuniarti K. Pumpun Yuspita, Ni Luh Eta