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All Journal HAYATI Journal of Biosciences Teknosains: Media Informasi Sains dan Teknologi Folia Medica Indonesiana Biology, Medicine, & Natural Product Chemistry Agrokreatif Jurnal Ilmiah Pengabdian kepada Masyarakat Jurnal Penelitian Pendidikan IPA (JPPIPA) EKONOMIS : Journal of Economics and Business Majalah Farmaseutik JMM (Jurnal Masyarakat Mandiri) SELAPARANG: Jurnal Pengabdian Masyarakat Berkemajuan Indonesian Journal of Biology Education Jurnal Kreativitas PKM Care : Jurnal Ilmiah Ilmu Kesehatan JPBIO (Jurnal Pendidikan Biologi) Abdimas Umtas : Jurnal Pengabdian kepada Masyarakat Community Development Journal: Jurnal Pengabdian Masyarakat Indonesia Berdaya Nusantara Science and Technology Proceedings Darmabakti : Junal Pengabdian dan Pemberdayaan Masyarakat MAHESA : Malahayati Health Student Journal Jurnal Abdimas Sangkabira Molucca Medica Jurnal Pengabdian Masyarakat : Pemberdayaan, Inovasi dan Perubahan Jurnal PADE: Pengabdian & Edukasi
Melda Yunita
Departemen Mikrobiologi Fakultas Kedokteran, Universitas Pattimura
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Journal : Indonesian Journal of Biology Education

 1 
Measurement of alpha-Amilase Enzymes in Bacillus subtilis Bacteria Sukmawati Sukmawati; Melda Yunita; Febrianti Rosalina
Indonesian Journal of Biology Education Vol 3, No 2 (2020): INDONESIAN JOURNAL OF BIOLOGY EDUCATION
Publisher : Universitas Tidar

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (355.601 KB) | DOI: 10.31002/ijobe.v3i2.3237

Abstract

The bacterium Bacillus subtilis is able to produce amylase enzymes which are able to hydrolyze various types of starch sources into simple compounds such as maltose, glucose. Amylase converts carbohydrates which are polysaccharides into maltose. Amylase enzyme is a group of enzymes that are needed in the industrial field. The purpose of this study was to determine the level of the amylase enzyme produced by the bacterium Bacillus subtilis. The method used in this research is a quantitative method using an amylase enzyme measurement experiment. The results of this study are from two types of enzyme sources, namely Bacillus subtilis + Starch + NB and Bacillus subtilis + NB. Both samples showed enzyme activity. The conclusion of this study is that Bacillus subtilis + Starch + NB produces 63.186 units of the alpha-amylase enzyme used to produce 1 ppm / 1 micromol maltose per minute. Whereas the Bacillus subtilis + NB sample was 19.474 units of the alpha-amylase enzyme used to produce 1 ppm / 1 micromol maltose per minute.
Co-Authors Abbas, Muthmainnah Ade Nurhayati Kusumadewi Adelia Mantong Alessandra Flowrence Saija Alimudi, Saiful Anggun Lestari Husein Astuti Nur Fadhilah ASTUTI, EKA Astuty, Eka Augie Desvie Klausya Kadun B. Bension, Johan Bandjar, Fitri Kadarsih Bandjar, Fitri Kardasih Cucu Hodijah de Lima, Filda V.I. de Lima, Filda Vionita Irene Dilya Maghfirah Taher DRIANA, Elin Dylan Tamalsir Dylan Tamalsir Effendi Effendi Eka Astuti Eka Astuty Elpira Asmin Elpira Asmin Elpira Asmin Erin D Noya Ernawati Ernawati Esmeraldine, Genevieva Etrin Zulqarnain Eka Saputra Linggar Evangelista Risalia Haurissa Fadila Tri Starlia Farah Christina Noya Filda de Lima Grace Latuheru Halidah Rahawarin Hartono Nurlette Hasibuan, Andrian Bayo Arif Hasna Vuspita Latuconsina Hataul, Is Asma’ul Haq Henok Siboka Onim Hursepuny, Valentine Husein, Anggun Lestary Ilsan, Noor Andryan Indas Wari Rahman Inggrain, Maulin Inggraini, Maulin Intania Riska Putrie, Intania Riska Irwan Ivanmorl Ruspanah Jennifer Vivian Kiriwenno Jepri Agung Priyanto, Jepri Agung Johan B Bension Johan Bruyif Bension Kusnara, Hasti Pramesti Latuheru, Grace Lesbatta, Kezia Josawel Mailoa, Joice Mainase, Josepina Makatita, Miftah H Marasabessy, Rendi Marasabessy, Rendy Mariyatul Qibtiyah Maxwell Landri Vers Malakauseya Metekohy, Meilani Mus, Rosdiana Musaid, Hendra Mutmainnah Abbas Myllisa Citra Wulandari Nahumarury, Salmin Nahumarury, Salmin Safril Noviyanter Siahaya Noya, Farah C Nurdin, Sukmawati Nurul Kusuma Dewi Ohiwal, Morgan Patteilohy, Merry Presly G. Siahaya Rachmawati Dwi Agustin Rahawarin, Halidah Ramanda, Viqih Ramla, Razita Ratau, Asria Reubun, Ririn Jihan S Rosalina, Febrianti Rumatiga, Masdar Ruth Magdalena Lumbantobing Safinah Yulianty Sitania Sipriyadi Siti Nur Azizah Siti Nurfajriah Sofia Mustamu Soumena, Rifah Zafarani Sukmawati Sukmawati Sukmawati Sukmawati Sukmawati, Sukmawati Sulfiana Sulfiana SULFIANA, SULFIANA Tahitu, Ritha Talakua, Gratia Tamtelatihu, Carmila L Titin Agustina Titin Agustina Umban Adi Jaya Usman Umarella Valentine Hursepunny Victor Leo Nolly Kewilaa Vina Z. Latuconsina Warella, Juen Carla Yensy Lolita Salamor Yosephina Manse Yuliawati , Sri Yuniasih Mulyani Jubeliene Taihuttu