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All Journal HAYATI Journal of Biosciences Teknosains: Media Informasi Sains dan Teknologi Folia Medica Indonesiana Biology, Medicine, & Natural Product Chemistry Agrokreatif Jurnal Ilmiah Pengabdian kepada Masyarakat Jurnal Penelitian Pendidikan IPA (JPPIPA) EKONOMIS : Journal of Economics and Business Majalah Farmaseutik JMM (Jurnal Masyarakat Mandiri) SELAPARANG: Jurnal Pengabdian Masyarakat Berkemajuan Indonesian Journal of Biology Education Jurnal Kreativitas PKM Care : Jurnal Ilmiah Ilmu Kesehatan JPBIO (Jurnal Pendidikan Biologi) Abdimas Umtas : Jurnal Pengabdian kepada Masyarakat Community Development Journal: Jurnal Pengabdian Masyarakat Indonesia Berdaya Nusantara Science and Technology Proceedings Darmabakti : Junal Pengabdian dan Pemberdayaan Masyarakat MAHESA : Malahayati Health Student Journal Jurnal Abdimas Sangkabira Molucca Medica Jurnal Pengabdian Masyarakat : Pemberdayaan, Inovasi dan Perubahan Jurnal PADE: Pengabdian & Edukasi
Melda Yunita
Departemen Mikrobiologi Fakultas Kedokteran, Universitas Pattimura
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Journal : HAYATI Journal of Biosciences

 1 
Genetic Background of β-Lactamase Genes in Extraintestinal Pathogenic Escherichia coli ST131 in Indonesia Based on Whole-Genome Sequencing (WGS) Sequences Noor Andryan Ilsan; Sipriyadi Sipriyadi; Melda Yunita; Siti Nurfajriah; Maulin Inggraini
HAYATI Journal of Biosciences Vol. 29 No. 4 (2022): July 2022
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.29.4.540-548

Abstract

Extraintestinal pathogenic Escherichia coli (ExPEC) is a group of pathogens that can colonize the outside of the intestine, such as the kidney, urinary tract, or bloodstream. This study analyzed more about the genetic background of β-lactamase genes among Indonesian ExPEC ST131. Whole-genome sequencing (WGS) sequences from the National Center for Biotechnology Information (NCBI) of Indonesian ExPEC ST131 were taken, then analyzed. Circular genomic mapping and genomic comparison of surrounding genes of β-lactamase in these isolates were generated. blaOXA-1 and blaDHA-1 were analyzed deeply due to their presence in a relatively long contig, making them available for analysis. Indonesian ExPEC ST131 isolates had blaOXA-1 with an identical genetic background of E. coli originating from China and Austria with aac (6’)-1b-cr5 in the downstream and cab83 in the upstream. The blaOXA-1 was also detected in other species, including Klebsiella pneumoniae INF142 originating from Australia and Salmonella enterica S146 from China. While, blaDHA-1 in EC_0406 had an identical genetic background to E. coli 142 and other species such as Shigella sonei FC1428 from India and S. flexneri M2901 from Australia, with Globulin-encoded gene in the upstream and lysR in the downstream. Our findings demonstrate the global spread of β-lactamase genes detected in Indonesian ExPEC ST131. These genes were identical to those isolated from some countries around the world.
Virulence Evaluation of Aeromonas spp. KS-1 Isolated from Kitchen Sponge using Omphisa fuscidentalis Larvae Ilsan, Noor Andryan; Inggrain, Maulin; Nurfajriah, Siti; Yunita, Melda; Priyanto, Jepri Agung; Ramanda, Viqih
HAYATI Journal of Biosciences Vol. 31 No. 4 (2024): July 2024
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.31.4.613-620

Abstract

Aeromonas spp. causes the human diseases including diarrhea, gastroenteritis, and bacteremia. Aeromonas spp. can be found in kitchen sponge, one of the reservoirs for food-borne bacterial pathogens. Virulence study of Aeromonas spp. in vivo in animal model is important since the animal model can mimic manifestasions in human infections. Omphisa fuscidentalis was chosen for alternative virulence model, since they are in the same taxonomical order with the well-known infections model, Galleria mellonella. Bacterial isolation and selection of kitchen sponge used Brain Heart Infusion agar and Endo Agar, respectively. Bacterial virulence of KS-1 was injected into Omphisa fuscidentalis larvae. Survival percentage and melanization score of infected larvae were evaluated. Hemolymph of larvae with melanization score of 1 and 4 were stained with Giemsa method to observe the hemocyte changes. Bacterial identification of isolate KS-1 based on 16S rRNA sequence resulted in 96.9% identity to Aeromonas spp. strain VS7. Isolate KS-1 injection to O. fuscidentalis revealed higher bacterial dosage resulting more severe symptoms to the larvae according to survival percentage and melanization score. However, statistical analysis showed evaluation of melanization score could distinguish larvae with 106 and 107 CFU/larva dosage injection, while evaluation of survival percentage could not. Hemocyte of larvae with melanization score 1 had larger and more cytoplasmic vacuolization than the score 4 (healthy larvae). Omphisa fuscidentalis is an alternative of insect model for bacterial infections with survival percentage and melanization score as the evaluation. Cytoplasmic vacuolization of hemocyte can be used as larvae’s health indicator in a cellular level.
Co-Authors Abbas, Muthmainnah Ade Nurhayati Kusumadewi Adelia Mantong Alessandra Flowrence Saija Alimudi, Saiful Anggun Lestari Husein Astuti Nur Fadhilah ASTUTI, EKA Astuty, Eka Augie Desvie Klausya Kadun B. Bension, Johan Bandjar, Fitri Kadarsih Bandjar, Fitri Kardasih Cucu Hodijah de Lima, Filda V.I. de Lima, Filda Vionita Irene Dilya Maghfirah Taher DRIANA, Elin Dylan Tamalsir Dylan Tamalsir Effendi Effendi Eka Astuti Eka Astuty Elpira Asmin Elpira Asmin Elpira Asmin Erin D Noya Ernawati Ernawati Esmeraldine, Genevieva Etrin Zulqarnain Eka Saputra Linggar Evangelista Risalia Haurissa Fadila Tri Starlia Farah Christina Noya Filda de Lima Grace Latuheru Halidah Rahawarin Hartono Nurlette Hasibuan, Andrian Bayo Arif Hasna Vuspita Latuconsina Hataul, Is Asma’ul Haq Henok Siboka Onim Hursepuny, Valentine Husein, Anggun Lestary Ilsan, Noor Andryan Indas Wari Rahman Inggrain, Maulin Inggraini, Maulin Intania Riska Putrie, Intania Riska Irwan Ivanmorl Ruspanah Jennifer Vivian Kiriwenno Jepri Agung Priyanto, Jepri Agung Johan B Bension Johan Bruyif Bension Kusnara, Hasti Pramesti Latuheru, Grace Lesbatta, Kezia Josawel Mailoa, Joice Mainase, Josepina Makatita, Miftah H Marasabessy, Rendi Marasabessy, Rendy Mariyatul Qibtiyah Maxwell Landri Vers Malakauseya Metekohy, Meilani Mus, Rosdiana Musaid, Hendra Mutmainnah Abbas Myllisa Citra Wulandari Nahumarury, Salmin Nahumarury, Salmin Safril Noviyanter Siahaya Noya, Farah C Nurdin, Sukmawati Nurul Kusuma Dewi Ohiwal, Morgan Patteilohy, Merry Presly G. Siahaya Rachmawati Dwi Agustin Rahawarin, Halidah Ramanda, Viqih Ramla, Razita Ratau, Asria Reubun, Ririn Jihan S Rosalina, Febrianti Rumatiga, Masdar Ruth Magdalena Lumbantobing Safinah Yulianty Sitania Sipriyadi Siti Nur Azizah Siti Nurfajriah Sofia Mustamu Soumena, Rifah Zafarani Sukmawati Sukmawati Sukmawati Sukmawati Sukmawati, Sukmawati Sulfiana Sulfiana SULFIANA, SULFIANA Tahitu, Ritha Talakua, Gratia Tamtelatihu, Carmila L Titin Agustina Titin Agustina Umban Adi Jaya Usman Umarella Valentine Hursepunny Victor Leo Nolly Kewilaa Vina Z. Latuconsina Warella, Juen Carla Yensy Lolita Salamor Yosephina Manse Yuliawati , Sri Yuniasih Mulyani Jubeliene Taihuttu