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All Journal Microbiology Indonesia Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Biomedical Engineering Indonesian Journal of Tropical and Infectious Disease WMJ (Warmadewa Medical Journal) YARSI Medical Journal Biomolecular and Health Science Journal Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCPML) Indian Journal of Forensic Medicine & Toxicology Jurnal Keperawatan Soedirman (The Soedirman Journal of Nursing) Folia Medica Indonesiana
Retno Handajani
Department Of Biochemistry, 00Faculty Of Medicine, Universitas Airlangga, Surabaya
Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Nursing Public Health

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Journal : biomedical engineering

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Detection of Hepatitis C Virus RNA in Blood Donors with Nested PCR Technique Supiana Dian Nurtjahyani; Mochammad Amin Amin; Retno Handajani Handajani
Biomedical Engineering Vol 2, No 2 (2016): Biomedical Engineering
Publisher : Biomedical Engineering

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Abstract

Objective: This study was conducted to detect hepatitis C virus (HCV) RNA among blood donors in Tuban, East Java, Indonesia,  using nested Polymerase Chain Reaction (PCR) technique.Material and methods: Seven blood donors  with positives anti-HCV were obtained from 500 blood donors from Red Cross Blood Donor Center (Palang Merah Indonesia = PMI) Tuban, East Java, Indonesia, consisting of 375 men (75%) and 125 women (25%). Detection of anti-HCV antibody prevalence in the blood donors was done by using Enzyme-Linked Immunosorbent Assay (ELISA).  The detection of HCV RNA in blood donors  sera  with positives  anti-HCV were performed using nested-PCR technique with three set of primer pairs targeting the NS5B and 5’UTR regions of HCV.  Further amplification products were visualized on a 2% agarose gel containing ethidium bromide under  UV illumination.Result: Seven positives anti-HCV blood donors sera (1.4%) were obtained from 500 blood donors sera, in PMI Tuban, East Java, Indonesia. Out of seven blood donors sera with positives anti-HCV, 6 sera (85,7%)  were positives HCV RNA by using nested PCR technique. Five HCV RNAs were detected with two set of primers based on NS5B HCV region and 1 positive HCV RNA was detected with another set of primer based on 5’UTR HCV region.Conclusion: HCV RNAs were detected in 85,7% positives anti-HCV blood donors using nested PCR technique, in PMI Tuban, East Java. Further research is needed to determine the  genotype  and  subtype  of  HCV. 
Detection of Gen Chloramphenicol Acetyl Transferase (CAT ) Salmonella typhi Resistant Chloramphenicol by Polymerase Chain Reaction (PCR) Technique Supiana Dian Nurtjahyani; Retno Handajani
Biomedical Engineering Vol 3, No 1 (2017): Vol. 3 No. 1 Biomedical Engineering
Publisher : Biomedical Engineering

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Objective: Salmonella typhi as a causative agent of typhoid abdominalis many militias, which was resistant to the drug, one of which is chloramphenicol. Detection of Salmonella typhi serologically generally done so if there is a problem in the diagnosis difficult to overcome so it needs a faster diagnosis techniques with accurate results. PCR can be used as an alternative solution to these problems. The purpose of this study was to detect the DNA of Salmonella typhi resistant to chloramphenicol by PCR.Material and methods: The method in this population experimental laboratory conducted in the laboratory biology and laboratory Unirow Tuban ITD Airlangga University Surabaya. Experiment done using PCR from blood samples. Using primer from CAT gene to detect this gene.Result: The results of this study with tank PCR can detect DNA of chloramphenicol resistant Salmonella typhi with a length of 293 bp.Conclusion: The conclusion PCR technique can detect DNA of Salmonella typhi resistant to chloramphenicol.
Co-Authors A Kentjono, Widodo Adi, Ginanjar Sasmito Afrina Zulaikah Amin, Indah Binti Mohd Anggraeni, Zuhrotul Eka Yulis Ayesie Natasha Zulka B Notopuro, Paulus Bakhtiar, Arief Bakhtiar, Arief Bayu Satria Wiratama David S. Perdanakusuma Edhi Rianto Elvaretta, Clarissa Ely Rahmatika Nugrahani Ely Rahmatika Nugrahani, Ely Rahmatika Fis Citra Ariyanto Gatut Suhendro Ginanjar Sasmito Adi HAK HOTTA Harianto Notopuro Hendri Susilo Hendy Muagiri Margono Henydhar Bramastivira Mahdani Hernomo Ontoseno Kusumobroto Humairah, Ira I Gusti Agung Komang Diafari Djuni Hartawan Indri Safitri Indri Safitri, Indri Juniastuti Juniastuti Kawilarang, Arthur Pohan Khaerunnisa, Siti Luki Indriaswati Lukitasari, Lina Lukitasari, Lina Margarita Maramis, Margarita Maria Inge Lusida MARIA INGE LUSIDA MOCHAMAD AMIN MOCHAMAD AMIN Mochamad Amin Mochamad Yusuf Alsagaff Mochammad Amin MOCHAMMAD THAHA Mochammad Thaha Nurwasis Nurwasis Nyilo Purnami Palupi, Dian Eka Roro Poernomo Boedi Setiawan Prabowo, Gwenny Ichsan Pranawa Pranawa Priyo Budi Purwono Qurnianingsih, Ema Radias Dwi Padmani Rizaliyana, Sitti Ruby Riana A Ruby Riana Asparini Ruslan, S. Eriaty N. S. Perdanakusuma Samira Assegaf Siti Khaerunnisa SOETJIPTO SOETJIPTO . SOETJIPTO . Soetjipto Soetjipto Sulistyo Mulyo Agustini Supiana Dian Nurtjahyani Susi Wahyuning Asih Susi Wahyuning Asih, Susi Wahyuning Suwandito Suwandito Suwandito, Suwandito Syafa'ah, Irmi Syafa’ah, Irmi TAKAKO UTSUMI Ulfa Kholili Ummah, Fithriyah Cholifatul Ummi Maimunah Waskito, Langgeng Agung Wiwik Misaco Yuniarti Wungu, Citrawati Dyah Kencono Wungu, Citrawati Dyah Kencono YOSHIHIKO YANO YOSHITAKE HAYASHI Yulianti, Erikavitri Zain Budi Syulthoni Zuhrotul Eka Yulis Anggraeni Zulka, Ayesie Natasha