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MORFOLOGI DAN INTEGRITAS INTI KEPALA SPERMATOZOA SAPI SIMENTAL PASCA LIOFILISASI DENGAN WAKTU adiansyah, adiansyah; handarini, Ristika
Jurnal Peternakan Nusantara Vol 1, No 1 (2015)
Publisher : Universitas Djuanda Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (582.491 KB) | DOI: 10.30997/jpnu.v1i1.171

Abstract

Pengeringbekuan merupakan salah satu teknologi pilihan dalam pengawetan sel dan telah diaplikasikan secara luas termasuk sel spermatozoa. Penelitian ini bertujuan untuk pelihat perubahan morfometri dan integritas inti kepala spermatozoa sapi pasca liofilisasi dengan waktu inkubasi yang berbeda. Rancangan penelitian yang digunakan rancangan acak faktorial 2 faktor, faktor pertama: sumber semen (semen segar tanpa liofilisasi, semen segar liofilisasi dan semen beku liofilisasi) dan faktor kedua: lama inkubasi (0, 3 dan 6 jam). Uji ANOVA dilanjutkan dengan uji Duncan bila terdapat perbedaan nyata atau sangat nyata (SPSS 16). Peubah yang diamati: morfometri sperma (panjang, lebar dan luas kepala spermatozoa) dan integritas spermatozoa. Hasil penelitian menunjukkan rataan morfometri spermatozoa segar: panjang (9.29 μm), lebar (4.78 μm) dan luas kepala spermatozoa (36.59 μm2) secara signifikan mengecil (P<0.05) setelah diikubasi selama 3 jam yaitu panjang (9.10 μm), lebar (4.72 μm) dan luas kepala spermatozoa (35.72 μm2). Pada inkubasi 6 jam menunjukkan perbedaan tidak nyata (P>0.05) terhadap panjang (9.24 μm), lebar (4.73 μm) dan luas kepala spermatozoa (35.89 μm2). Sperma segar dan beku yang diliofilisasi dan diinkubasi selama 3 dan 6 jam menunjukkan morfometri yang tidak berbeda nyata (P>0.05). Data inimenunjukkan bahwa ada upaya sperma mempertahankan diri pada lingkungan baru selama 3 jam dan menjadi normal kembali ukurannya pada inkubasi selama 6 jam. Integritas inti kepala spermatozoa tidak berbeda nyata (P>0.05) pada inkubasi 0, 3 dan 6 jam. Integritas inti sperma segar yang diliofilisasi menunjukkan tidak berbeda nyata (P>0.05) pada inkubasi 3 jam, namun berbeda nyata (P<0.05) pada inkubasi 6 jam. Integritas inti sperma beku yang diliofilisasi setelah diinkubasi selama 6 jam mengalami penurunan drastis (P<0.05) yaitu 42% intak. Kesimpulan dari penelitian ini adalah integritas inti sperma segar tanpa liofilisasi stabil setelah diinkubasi selama 6 jam, sementara sperma segar dan beku yang diliofilisasi mengalami penurunan pada integritas inti.
PENGARUH SUBSTITUSI PAKAN KOMERSIL DENGAN TEPUNG AMPAS KELAPA TERHADAP PERFORMA AYAM KAMPUNG kesatria, Kesatria; Handarini, Ristika
Jurnal Peternakan Nusantara Vol 2, No 1 (2016)
Publisher : Universitas Djuanda Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (603.095 KB) | DOI: 10.30997/jpnu.v2i1.362

Abstract

Ampas kelapa merupakan limbah yang masih memiliki kandungan nutrisi yang baik, sehingga berpotensi dijadikan pakan ternak ayam khususnya ayam kampung. Penelitian ini bertujuan untuk mengkaji performa ayam kampung dengan mensubstitusikan sebagian pakan komersil dengan tepung ampas kelapa. Penelitian dilaksanakan dari 17 September sampai 31 Oktober 2015 di Laboratorium Ternak Unggas, Program Studi Peternakan, Universitas Djuanda Bogor. Ternak yang digunakan dalam penelitian ini adalah day old chick ayam kampung, sebanyak 100 ekor. Penelitian menggunakan rancangan acak lengkap (RAL) yang terdiri atas 5 perlakuan dan 4 ulangan, Perlakuan dalam penelitian ini adalah R0: 100% pakan komersil, R1: 90% pakan komersil + 10 % tepung ampas kelapa, R2: 80% pakan komersil + 20 % tepung ampas kelapa, R3: 70% pakan komersil + 30 % tepung ampas kelapa, R4: 60% pakan komersil + 40% tepung ampas kelapa. Data yang diperoleh dianalisis menggunakan Analisys of Variance (ANOVA) dan uji Duncan. Peubah yang diamati adalah konsumsi pakan, pertambahan bobot badan, konversi pakan dan mortalitas. Hasil penelitian menunjukan bahwa konsumsi pakan ayam kampung selama penelitian tidak berbeda nyata (P > 0,05), pertambahan bobot badan menunjukan perbedaan yang nyata (P < 0,05) pada P1 dengan P2 dan P4.Konversi pakan pada P0 dan P1 berbeda nyata (P < 0,05) dengan konversi pakan pada P2 dan P4. Subtitusi ransum komersil dengan tepung ampas kelapa dapat dilakukan sampai tingkat 30% tanpa mengurangi performa ayam kampung.
ESTRUS RESPONSE HOLSTEIN FRIESIAN COW PERANAKAN WHO INJECTED PROSTAGLANDIN IN INTRAMUSCULAR AND INTRAUTERINE Nuryanto, Lilik Bawa; Handarini, Ristika; Setiawan, Yanyan
Jurnal Peternakan Nusantara Vol 3, No 2 (2017)
Publisher : Universitas Djuanda Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (626.608 KB) | DOI: 10.30997/jpnu.v3i2.929

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This study was aimed to compare the different responses of cattle estrus and pregnancy rate FH (Friesian Holsein) were injected with the prostaglandin hormone by intramuscular and intra-uterine. This study was used 20 cows FH on condition of normal reproductive function, not in a state of pregnant (confirmed by rectal palpation), visually not fat and not skinny, not reproductive disease, normal estrous cycles. Cows were divided into two treatment, P1: estrus synchronization by intra muscular (IM), P2: estrus synchronization by  intrauterine (IU). Data were analyzed by T test, to compare the differences between the two treatments with SPSS device. The results showed that the synchronization of pregnancy by using intrauterine more efisien. The conclusion of this research PGF2α hormone injections by intrauterinehas atendency response   higher than by intra muscular injection method.               Keyword : Non Return Rate, Service Per Conception, Conception Rate.
THE QUALITY OF EMBRYOS RESULTED FROM IN VITRO FERTILIZATION BY USING FROZEN SEMEN THAWED IN DIFFERENT TEMPERATURES Yasri, Rakhmi; Handarini, Ristika; Imron, Muhammad
Jurnal Peternakan Nusantara Vol 3, No 1 (2017)
Publisher : Universitas Djuanda Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (501.711 KB) | DOI: 10.30997/jpnu.v3i1.853

Abstract

In vitro fertilization technology in cows is an effort done to utilize ovary waste from cows slughtered in abbatoir. This study was aimed at assessing the qualiy of embryos resulted from in vitro fertilization by using frozen semen thawed in different temperatures. In order to get qualty semen, standardized thawing method is required. It was expected from this study that an optimum thawing temperature for frozen semen was determined to obtain quality transferable embryos. Three treatments consisting of thawing with water 37°C for 30 second (T1), thawing with water 25°C for 30 second (T2), and thawing with water 10°C for 30 second (T3). Data were subjected to an an anlysis of variance (Anova) and a Duncan test. Results showed that oocytes fertilized with frozen semen thawed at 37°C and 10°C had higher fertilization rate and excellent-grade embryos (P<0.05) than did the ones fertilized with frozen semen thawed at 25°C. However, no different effect of thawing temperatures was found on transferable and degenerated embryos (P>0.05). It was concluded that embryos fertilized with Brahman frozen semen in thawed at 37°C had the highest number of embryos (49.66±2.88) and excellent-grade embryos (22.00±4.35). Key words: Embryo quality, In vitro fertilization, frozen semen thawing, Brahman bull.
EFEKTIVITAS PENYUNTIKAN FSH SECARA SUBKUTAN DAN INTRAMUSKULAR TERHADAP RESPON SUPEROVULASI SAPI SIMENTAL Djunaedi, Mohammad; Handarini, Ristika; Zamanti, Deasy
Jurnal Peternakan Nusantara Vol 4, No 1 (2018): Jurnal Peternakan Nusantara
Publisher : Universitas Djuanda Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (23.63 KB) | DOI: 10.30997/jpnu.v4i1.1562

Abstract

Superovulation is a necessary technique to produce large number of embryos for embryo transfer. Hormonal treatment is superovulation methode can be done by implant CIDR and injection of Follicle Stimulating Hormone (FSH). Experiment were carried out to observe the effectiveness of subcutaneous and intramuscular FSH injections on superovulation response in simmental cattle. All animal (n=10) were treated with intravaginal CIDR implant before FSH injection. Studies were devided into two experiment. In experiment 1 (P1), 400 mg FSH (diluted in 4 ml sterile diluent) injected in five simmental cattle by single subcutan injection. In experiment 2 (P2), 400 mg FSH (diluted in 20 ml sterile diluent) injected in five simmental cattle by twice daily intramuscular injection over 4 day in decreasing doses. The number of corposa luteal (CL), total embryos collected, and total transferable embryos were observed in this experiment. Data were analyzed by T-test method. The result showed that effectiveness of single subcutan FSH injection were significanthy different (P < 0,05) than intramuscular FSH injection superovulation with single subcutan FSH injetcion is easier than twice daily intramuscular injection in decreasy dose. In conclusion the average of CL (21,4 ± 3,6) and number of tranferable embryo (71,96 %) of the single subcutan FSH injection tended to be better than intramuscular FSH injection. Single subcutan FSH injection more efficient than intramuscular FSH injection. Single subcutan injection can decreasing sterss level in the cattle and be easier in handling the cattle during the experiment.Keywords: Simmental cattle, FSH, superovulation, single subcutan injection, intramuscular injection.
PERSENTASE BAGIAN-BAGIAN KARKAS ITIK LOKAL JANTAN YANG RANSUMNYA DITAMBAH LARUTAN DAUN SIRIH (PIPER BETLE LINN) DAN BUNGA KECOMBRANG (ETLINGERA ELATIOR) PERCENTAGES OF CARCASS AND ITS PARTS OF MALE LOCAL DUCKS FED PIPER BETLE LEAF AND TORCH GINGER FLOWER SOLUTION INCLUDED IN COMMERCIAL RATION solihin, solihin; Handarini, Ristika; Dihansih, Elis
Jurnal Peternakan Nusantara Vol 4, No 1 (2018): Jurnal Peternakan Nusantara
Publisher : Universitas Djuanda Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (23.63 KB) | DOI: 10.30997/jpnu.v4i1.1533

Abstract

This study was aimed at assessing the effects of the inclusion of piper betle leaf and torch ginger flower solution in commercial ration on the percentages of carcass and its parts of male local ducks. The study was conducted from June to August 2016 at Poultry Laboratory of Animal Science Study Program, Faculty of Agriculture, Djuanda University, Bogor. Twenty-four male local ducks aged 2 weeks with average initial body weight of 450 ± 53.04 g were used. A completely randomized design with 4 treatments and 3 replicates was used. Treatments consisted of 100% commercial ration (R0), commercial ration +2.5% piper betle leaf solution + 2.5% torch ginger flower solution (R1), commercial ration+ 5.0% piper betle leaf solution+ 2.5% torch ginger flower solution (R2), and commercial ration + 7.5% piper betle leaf solution + 2.5% torch ginger flower solution (R3). Measurements were taken onlive weight, the percentages of carcass, breast, wings, thigh, and back. Data were subjected to an analysis of variance and a Duncan test. Results showed that the inclusion of piper betle leaf and torch ginger flower solution in the rations of male local ducks gave significant effects (P<0.05) on the percentages of wings and thigh. Treatments were also found to maintain the percentages of breast and back. The inclusion of 2,5% piper betle leaf solution and 2,5% torch ginger flower solution in ration was also found to increase the percentage of thigh of male local ducks. It was recommended that 2,5% piper betle leaf and torch ginger flower solution be included in ration to result in higher percentages of and more economical wings and thigh.
PERSENTASE KARKAS DAN POTONGAN KOMERSIAL ITIK LOKAL JANTAN YANG DIBERI LARUTAN BUNGA KECOMBRANG DALAM PAKAN Komara, Fatutthorman; nur, Hanafi; Handarini, Ristika
Jurnal Peternakan Nusantara Vol 4, No 1 (2018): Jurnal Peternakan Nusantara
Publisher : Universitas Djuanda Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30997/jpnu.v4i1.1561

Abstract

Torch ginger flower which functions as antibiotic and antioxidant is potential to be used as feed additive in ration to improve local duck production.  This study was aimed at assessing the effects of the inclusion of torch ginger flower solution in rations on the percentages of carcass and meat commercial cuts of male local ducks.  The study was conducted at Poultry Laboratory of Department of Animal Husbandry, Faculty of Agriculture, Djuanda University, Bogor from June to August 2016.  Twenty-four male local ducks aged 2 weeks with mean initial body weight of 450±70.35 g were used.  Ducks were fed basal rations containing 20–22% crude protein.    Torch ginger flower solution was included in the rations.  Ducks were allocated into battery cages of 150 x 40 cm each.  A completely randomized design with 4 treatments and 3 replicates was used.  Treatments consisted of commercial ration (PK) + 0% torch ginger flower solution (LBK) (R1), PK + 2.5% LBK (R2), PK + 5% LBK (R3), and PK + 7.5% LBK (R4).  Measurements were taken on the percentage of carcass and meat commercial cuts (wing, breast, back, thigh).  Data were subjected to an analysis of variance and a Duncan test.  Results showed that treatments gave significant effects (P<0.05) on carcass percentage but not on meat commercial cuts.  It was concluded that the inclusion of torch ginger flower solution in rations of up to 7.5% increased carcass percentage and tended to increase breast and thigh percentages.  It was suggested that the inclusion of torch ginger flower solution in rations be done by 5% to result in higher and more economical duck meat commercial cuts (breast and thigh). Key words: carcass percentage, commercial cuts, male local duck, torch ginger flower solution
PERSENTASE KARKAS DAN POTONGAN KOMERSIAL ITIK LOKAL JANTAN YANG DIBERI LARUTAN BUNGA KECOMBRANG DALAM PAKAN Komara, Fatutthorman; nur, Hanafi; Handarini, Ristika
Jurnal Peternakan Nusantara Vol. 4 No. 1 (2018)
Publisher : Universitas Djuanda Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (645.752 KB) | DOI: 10.30997/jpnu.v4i1.1513

Abstract

Torch ginger flower which functions as antibiotic and antioxidant is potential to be used as feed additive in ration to improve local duck production.  This study was aimed at assessing the effects of the inclusion of torch ginger flower solution in rations on the percentages of carcass and meat commercial cuts of male local ducks.  The study was conducted at Poultry Laboratory of Department of Animal Husbandry, Faculty of Agriculture, Djuanda University, Bogor from June to August 2016.  Twenty-four male local ducks aged 2 weeks with mean initial body weight of 450±70.35 g were used.  Ducks were fed basal rations containing 20–22% crude protein.    Torch ginger flower solution was included in the rations.  Ducks were allocated into battery cages of 150 x 40 cm each.  A completely randomized design with 4 treatments and 3 replicates was used.  Treatments consisted of commercial ration (PK) + 0% torch ginger flower solution (LBK) (R1), PK + 2.5% LBK (R2), PK + 5% LBK (R3), and PK + 7.5% LBK (R4).  Measurements were taken on the percentage of carcass and meat commercial cuts (wing, breast, back, thigh).  Data were subjected to an analysis of variance and a Duncan test.  Results showed that treatments gave significant effects (P<0.05) on carcass percentage but not on meat commercial cuts.  It was concluded that the inclusion of torch ginger flower solution in rations of up to 7.5% increased carcass percentage and tended to increase breast and thigh percentages.  It was suggested that the inclusion of torch ginger flower solution in rations be done by 5% to result in higher and more economical duck meat commercial cuts (breast and thigh). Key words: carcass percentage, commercial cuts, male local duck, torch ginger flower solution 
CARCASS AND NON CARCASS PERCENTAGES OF 35 DAY-OLD MALE QUAILS (COTURNIX-COTURNIX JAPONICA) GIVEN DRINK CONTAINING MORINGA LEAF SOLUTION fitrah, halim; Handarini, Ristika; Dihansih, Elis
Jurnal Peternakan Nusantara Vol. 4 No. 2 (2018)
Publisher : Universitas Djuanda Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (740.651 KB) | DOI: 10.30997/jpnu.v4i2.1542

Abstract

Quail is potential to provide protein source but its meat production in Indonesia is still limited.  Thi study was aimed at assessing the effects of the inclusion of moringa leaf solution in dringking water on carcass and giblet percentages of quails.  The study was conducted at Badrupuyuh Farm, Sukajaya Village RT 01/02, Ciomas, Bogor Regency from October to December 2017.  One-hundred quail birds aged 7 days with average initial body weight of 18.04±0.508 g were used.  The birds were fed starter and grower commercial rations.  Moringa leaf solution was given through drinking water.  A completely randomized design with 4 treatments and 5 replicates was used.   Treatments consisted of drinking water containing no moringa leaf solution (R0), drinking water containing 5% moringa leaf solution (R1), drinking water containing 10% moringa leaf solution (R2), drinking water containing 15% moringa leaf solution (R3).  Data were subjected to an analysis of variance and a Duncan test. Measurements were taken on the percentage of carcass, liver, gizzard, and heart. Results showed that treatments gave significant effects on liver percentage but not on the percentages of carcass, heart, and gizzard.  It was concluded that the inclusion of moringa leaf solution of up to 10% in drinking water optimized carcass and non carcass percentages of male quails.  It was recommended that moringa leaf solution of up to10% be included in drinking water of male quails. Key words: male quail, carcass, giblet, moringa leaf solution.
PERSENTASE BAGIAN-BAGIAN KARKAS ITIK LOKAL JANTAN YANG RANSUMNYA DITAMBAH LARUTAN DAUN SIRIH (PIPER BETLE LINN) DAN BUNGA KECOMBRANG (ETLINGERA ELATIOR) Solihin, Solihin; Handarini, Ristika; Dihansih, elis
Jurnal Peternakan Nusantara Vol. 4 No. 1 (2018)
Publisher : Universitas Djuanda Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (757.639 KB) | DOI: 10.30997/jpnu.v4i1.1510

Abstract

This study was aimed at assessing the effects of the inclusion of piper betle leaf and torch ginger flower solution in commercial ration on the percentages of carcass and its parts of male local ducks. The study was conducted from June to August 2016 at Poultry Laboratory of Animal Science Study Program, Faculty of Agriculture, Djuanda University, Bogor. Twenty-four male local ducks aged 2 weeks with average initial body weight of 450 ± 53.04 g were used. A completely randomized design with 4 treatments and 3 replicates was used. Treatments consisted of 100% commercial ration (R0), commercial ration +2.5% piper betle leaf solution + 2.5% torch ginger flower solution (R1), commercial ration+ 5.0% piper betle leaf solution+ 2.5% torch ginger flower solution (R2), and commercial ration + 7.5% piper betle leaf solution + 2.5% torch ginger flower solution (R3). Measurements were taken onlive weight, the percentages of carcass, breast, wings, thigh, and back. Data were subjected to an analysis of variance and a Duncan test. Results showed that the inclusion of piper betle leaf and torch ginger flower solution in the rations of male local ducks gave significant effects (P<0.05) on the percentages of wings and thigh. Treatments were also found to maintain the percentages of breast and back. The inclusion of 2,5% piper betle leaf solution and 2,5% torch ginger flower solution in ration was also found to increase the percentage of thigh of male local ducks. It was recommended that 2,5% piper betle leaf and torch ginger flower solution be included in ration to result in higher percentages of and more economical wings and thigh.Key words: Betle leaf, ginger flower , male local duck, percentage of thigh.
Co-Authors A.A. Ketut Agung Cahyawan W Abdullah Baharun Agung Puji Haryanto Agus restu budimas Ahmad Faqih Amril, La Ode Anak Agung Gede Sugianthara Anas Alhifni Anggraeni Anggraeni Anggraeni Annisa Rahmi Annisa Rahmi, Annisa Anwar, Ghoitsa Zahra Khoerul Arti Yoesdiarti Baharun, Abdulah Bambang Purwantara Burhanudin Malik Burhanudin Malik Dede Kardaya Deden Sudrajat Dewi Wahyuni Djunaedi, Mohammad Dwi Purnama Putri Elis Dihansih Elis Dihansih Elis Dihansih Fauzan, Dhifa Hilmi Feilza Putri, Okta fitrah, halim Florana, Bella Florana, Bella Furqan Herdes, Ali Gagarin, Muhamad Yuri GONO SEMIADI Gumelar, Restu Haerina, Nina Haerina, Nina Hanafi Nur Helmi Haris Hirzi Hanifi, Hirzi Ichsan Pamungkas Indra Cahya Kusuma Jatmiko Jatmiko Jatmiko Kennie Cendekia Desnamrina kesatria, Kesatria kesatria, Kesatria Khodijah, Neng Siti Komara, Fatutthorman Kumalasari, Rafika Kurniasih, Desta Dwi Kurniawan, Sukurna Kurniawan, Sukurna M. Winugroho Mala Nurilmala Malik, Burhanudin Maman, Asep Masitoh Maulana, Tulus Mohammad Winugroho, Mohammad Muhammad Amin Muhammad Husein Muhammad Imron, Muhammad Mulyana, Dani Nuryanto, Lilik Bawa Nuryanto, Lilik Bawa Parida Permadani Pertiwi, Pirda Permadani Pertiwi, Pirda Parida Pujiharyanto, Agung R. Iis Arifiantini Raden Mohamad Herdian Bhakti Ramadhani, Dede Syahrul Resti Yeksyastuti Rimadhani, Farras Fajria Rizal, M. Saman Rohman, Fatkur Sale, Maria Sale, Maria Septiadi, Asep Septiadi, Asep Setiawan, Yanyan Setiawan, Yanyan Siregar, Nur Asyiah Siti Julaeha, Siti Solihin Solihin Subchan, Fariz Azka Subchan, Fariz Azka Sudrajat, Mashudy Suryana, Ahmad swito, swito swito, swito Tuty Laswardi Yusuf Umar, Sayed Viona Oktavia Wahyan, Dwiki Tri Satya Wahyan, Dwiki Tri Satya Wicaksono, Muhammad Abdiki Widiyantoro, Shena Wilmientje Marlene Mesang Nalley Wini Nahraeni Yasri, Rakhmi Yasri, Rakhmi Yoga Yuniadi Yuandhani, Rezky Zaman, Mohammad Badru Zamanti, Deasy Zandos, F Zulfikar Siregar