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All Journal Jurnal Veteriner Jurnal Ilmiah Mahasiswa Veteriner Jurnal Kedokteran Hewan Jurnal Medika Veterinaria Jurnal Agripet
Dwinna Aliza
Faculty Of Veterinary Medicine, Universitas Syiah Kuala
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Veterinary

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Journal : Jurnal Kedokteran Hewan

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UJI TOKSISITAS AKUT EKSTRAK ETANOL DAUN MALAKA (Phyllantus emblica) TERHADAP MENCIT (Mus musculus). (Acute Toxicity Test of Ethanolic Extract of Malaka (Phyllantus emblica) Leaves on Mice (Mus musculus)) T. Armansyah TR; Sudi Indriany; Amalia Sutriana; Rosmaidar Rosmaidar; Nuzul Asmilia; Budianto Panjaitan; Dwinna Aliza; Hamdan Hamdan
Jurnal Kedokteran Hewan Vol 10, No 2 (2016): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (85.831 KB) | DOI: 10.21157/j.ked.hewan.v10i2.5137

Abstract

ABSTRACT The aim of this research was to asses the acute toxicity of ethanolic extract of malaka leaves using lethal dose 50 (LD50) on mice (Mus musculus). Twenty male mice weighing between 20-30 g were randomly divided into 4 groups (group K1-K4) of 5 mice each. All mice in group K1, K2, K3, and K4 were administered ethanolic extract of malaka leaves with the dose of 2, 4, 8, and 16 g/kg bw, respectively. Single dose of ethanolic extract of malaka leaves were given by oral gavage prior to clinical observation . The observation period was 14 days post administration, for sign of toxicity symptom, weight loss, and mortality. The result showed that no mortality was observed in the experimental animals during this study. Slight reduction of body weight was observed in group K2, K3, and K4, and no toxicity sign was found during fourteen days of observation. The LD50 of ethanolic extract of malaka leaves was higher than 16 g/kg body weight, thus, the substance was practically non toxic substance.
Characterization of Inhibin from Culture and Non Culture of Granulose Cells for Monoclonal Antibody of Inhibin Production Amiruddin Amiruddin; Tongku Nizwan Siregar; Amalia Sutriana; Dwinna Aliza; T. Armansyah
Jurnal Kedokteran Hewan Vol 4, No 1 (2010): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (486.224 KB) | DOI: 10.21157/j.ked.hewan.v4i1.9789

Abstract

This study has long-term objectives to obtain immunogenic prototype that can be used to induce multiple ovulation in goats. Working steps of this study were begun with the collection of ovarium from goats, collection of granulose cells, culture of granulose and characterization of molecular weight and isoelectric point (pI) of inhibin protein of granulose cells obtained from culture and non-culture of granulose cells, and followed by preparation of monoclonal antibody toward inhibin. The results showed that inhibin isolated either from culture or non-culture of granulose cells produced a 32 kDa band. Molecular weight of inhibin was measured by Western Blot. The 32 kDa band of SDS PAGE product appeared on Western Blot result was inhibin molecules produced by granulose cells collected fom culture and non-culture of granulose cells that can be identified by Mab-inhibin. Product of IEF gel electrophoresis suggested that inhibin molecule collected from culture of granulose cells has no charge at isoelectric points ranging from 5-6, depends on its total amino acid composition.
Co-Authors . Nurliana Abdullah A. Muhammadar Aisyah Fadillah Tunnisa Alfin Oktarian Amalia Sutriana Amiruddin Amiruddin Arman Sayuti Asmawati Asmawati Awaluddin Awaluddin Bagus Dwijayanti Batubara, Agung Setia Budianto Panjaitan Budianto Panjaitan Budianto Panjaitan Budianto Panjaitan Budianto Panjaitan chiara giri puspa Cut Nila Thasmi Dasrul Dasrul Dedi Fazriansyah Putra Denny Irmawati Dian Masyitha Dian Pratiwi Erdiansyah Rahmi Erdiansyah Rahmi Fadli A. Gani Firdus Firdus Fitriani Fitriani Gholib Gholib Hafizuddin Hafizuddin Hafizuddin Hafizuddin Hamdan Hamdan Hamdani Budiman Hamdani Budiman Hamny Sofyan Hattanul Mulia Indra Sitorus Jesica Ramadhanita Juli Melia Ketut Adnyane Mudite Lingga Surya Maret Daulay M Nur Salim Mahdi Abrar Mira Ayu Lestari Hasibuan Muchlisin Zainal Abidin Muhammad Hanafiah Muhammad Jalaluddin Mulyadi Adam Muslim Akmal Nazaruddin N Nazarudin Nazarudin Nellita Meutia Nurhazimah Nurhazimah Nuzul Asmilia Rahmiwati R Rastina Rastina Razali Daud Roslizawaty Roslizawaty Rosmaidar Rosmaidar Samadi S Siti Aisyah Srihadi Agungpriyono Sudi Indriany Sugito Sugito Syafrizal S Syafruddin Syafruddin T Reza Ferasyi T. Armansyah T. Armansyah Tengku Muhammad Teuku Armansyah Tongku Nizwan Siregar Tongku Nizwan Siregar Tongku Nizwan Siregar Tongku Nizwan Siregar Ummu Balqis Wahyuni, Sri Yudha Fahrimal Zainuddin Zainuddin Zuhrawati Zuhrawati