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All Journal Jurnal Akuakultur Indonesia Jurnal Ilmu dan Teknologi Kelautan Tropis ILMU KELAUTAN: Indonesian Journal of Marine Sciences BULETIN OSEANOGRAFI MARINA Saintek Perikanan : Indonesian Journal of Fisheries Science and Technology Jurnal Pasir Laut Jurnal Perikanan dan Kelautan Management of Aquatic Resources Journal (Maquares) Journal of Aquaculture Management and Technology Journal of Fisheries Resources Utilization Management and Technology AL KAUNIYAH International Journal of Marine and Aquatic Resource Conservation and Co-existence Omni-Akuatika Jurnal Kelautan Nasional JURNAL METEOROLOGI DAN GEOFISIKA Telcomatics Jurnal Meteorologi dan Geofisika Jurnal Perikanan dan Kelautan
Agus Hartoko
Coastal Resources Management, Department Of Aquatic Resources, Faculty Of Fisheries And Marine Science Diponegoro University, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Earth & Planetary Sciences Education Electrical & Electronics Engineering Energy Environmental Science Immunology & microbiology Library & Information Science Materials Science & Nanotechnology Physics Social Sciences Other

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Journal : AL KAUNIYAH

 1 
Identification and Phylogenetic Analysis of Blue Swimming Crab (Portunus sp.) in The Northern Waters of Jepara Used DNA Barcoding Lazuardhi, Risqi; Sabdaningsih, Aninditia; Ayuningrum, Diah; Saputra, Suradi Wijaya; Hartoko, Agus; Haeruddin, Haeruddin
Al-Kauniyah: Jurnal Biologi Vol 18, No 2 (2025): AL-KAUNIYAH JURNAL BIOLOGI
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/kauniyah.v18i2.38705

Abstract

The Awur Bay and Kartini Beach in Jepara, Central Java, are fishing areas with blue swimming crab as a main catch. This study aims to examine the species of blue swimming crabs captured in the Northern Waters of Jepara from different locations, analyze the molecular characteristics based on gender differences, obtain GenBank accession numbers as genetic conservation, and construct their phylogenetic tree. The research used a quantitative descriptive method. Samples were collected using purposive sampling from fishermen's catches and selected based on the difference in crab claws and carapace color, with a total of 4 samples. The samples were extracted using a DNA extraction kit and tested using Polymerase Chain Reaction (PCR) to visualize mitochondrial DNA (COI gene) and processed using MEGA 11. BLAST results showed that the similarity of the samples to Portunus pelagicus ranged from 97.02% to 99.42%. The different colors of claws and carapace did not affect the DNA sequence composition, hence indicating sexual dimorphisms. The DNA sequences from this study were deposited to GenBank with accession number LC836055.1-LC836058.1. Based on the phylogenetic tree construction, the clade of all samples shows a close relationship with the species Portunus pelagicus.
Identification and Phylogenetic Analysis of Blue Swimming Crab (Portunus sp.) in The Northern Waters of Jepara Used DNA Barcoding Lazuardhi, Risqi; Sabdaningsih, Aninditia; Ayuningrum, Diah; Saputra, Suradi Wijaya; Hartoko, Agus; Haeruddin, Haeruddin
Al-Kauniyah: Jurnal Biologi Vol. 18 No. 2 (2025): AL-KAUNIYAH JURNAL BIOLOGI
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/kauniyah.v18i2.38705

Abstract

The Awur Bay and Kartini Beach in Jepara, Central Java, are fishing areas with blue swimming crab as a main catch. This study aims to examine the species of blue swimming crabs captured in the Northern Waters of Jepara from different locations, analyze the molecular characteristics based on gender differences, obtain GenBank accession numbers as genetic conservation, and construct their phylogenetic tree. The research used a quantitative descriptive method. Samples were collected using purposive sampling from fishermen's catches and selected based on the difference in crab claws and carapace color, with a total of 4 samples. The samples were extracted using a DNA extraction kit and tested using Polymerase Chain Reaction (PCR) to visualize mitochondrial DNA (COI gene) and processed using MEGA 11. BLAST results showed that the similarity of the samples to Portunus pelagicus ranged from 97.02% to 99.42%. The different colors of claws and carapace did not affect the DNA sequence composition, hence indicating sexual dimorphisms. The DNA sequences from this study were deposited to GenBank with accession number LC836055.1-LC836058.1. Based on the phylogenetic tree construction, the clade of all samples shows a close relationship with the species Portunus pelagicus.
Co-Authors - Pramonowibowo - Subiyanto - Supriharyono Abdul Ghani Abdul Ghofar Abdul Kohar Mudzakir Agung Wardana Agus Wahyudi Ahmad Fadlan Alfian Zulfikar, Alfian Ambariyanto Ambariyanto Andri Ramdhani Andrian Juniarta, Andrian Angela Merici Dwi Widiyanti Aninditia Sabdaningsih Anindya Wirasatriya Arif Rahman Aris Ismanto Ashari, Asqita Rakhma Ayuningtyas Indrawati Bagus Biantara, Bagus Bambang Sulardiono Boedi Hendrarto Budhi Agung Prasetyo Budianto, Tri Hendrawan Cahyo, Tri Nur Carleone de Prima, Carleone Churun A’in Damopolii, Sharfina Amalia Delianis Pringgenies Delis, Putu Cinthia Dewati Ayu Febrianti Dewi Sartika Diah Ayuningrum, Diah Djoko Suprapto Dwi Mulyasih EDY H.P. MELMAMBESSY Falensia, Talita Safa Febrianto, Sigit Ferdiansyah Ferdiansyah Fofied, Fernanda Gitarini Frida Aprilia Loinenak, Frida Aprilia Frida Purwanti Geertruidha Adelheid Latumeten Haeruddin Haeruddin Hanifati Masturah Haryani, Nisrina Septi Herman Yulianto Herry Boesono I. Kumalasari Indradi Setiyanto Isnawati, Nadiya Khoerul Umah Kitarake, Yopi Sondy Kukuh Prakoso, Kukuh Kurniawan Kurniawan Lahati, Sonny Lazuardhi, Risqi Lestari Lahksmi Widowati Lestari, Abid Lestari, Yumna Maro, Jahved Ferianto Max Rudolf Muskananfola Max Rudolf Muskanonfola Miladiyah Ahsanul Akhlak, Miladiyah Ahsanul Moh Toriq Abidin, Moh Toriq Monica Evi Suanty Manurung Monica Febrina Silitonga Muhammad Helmi Muhammad Najib Habibie Muhammad Zainuri Mutia Ismi Febrianti Mutia Ismi Febrianti Nabila Fikri Dwi Cahyani Nadhea O. Rahmawati Natalie, Gyorgiana Gilda Niniek Widyorini Nining Sari Ningsih Noky Rizky Samudra, Noky Rizky Nugroho, Restu Wahyu Nugroho, Tiyo Meizi Nurul Latifah nurul latifah Nurwinda Hikmawati Olvi Cristianawati Pinasthi, Laili Salma Pra Luber Agung Wibowo Pramonowibiwo M.Pi. Pratiwi, Galuh Alia Prayogi Prayogi Prijadi Soedarsono Pujiono Wahyu Purnomo Putri, Amanda Nerissa Qadar Hasani Rahayu Sapta S. Sudewi Rahmatuloh, Irzani Hamzah Setya Ralita, Tesya Feby Ratri Canar Perdana Restiana Wisnu Ariyati Richan Fakhrurrizal Rimty Mayuftia Rio Januardi, Rio Romadhony, Muchammad Yusuf Roni Kurniawan Rudy Kurniawan Sahala Hutabarat SARASWATI SARASWATI Sarif, Fina Saffuteri Sasanti Retno Suharti, Sasanti Retno Sembiring, Yoan Teresia Br Sibero, Mada T Simon Giando Sinaga, Simon Giando Siska Tri Cahyaningrum Siswanto Siswanto Siti Rudiyanti Sonny Lahati Sonny Lahati Subiyanto Subiyanto Suradi Wijaya Saputra Suryanti Suryanti - Sutrisno Anggoro Sutrisno Anggoro Syafrudin Syafrudin Urfan Ridha Wijayanti, Amelia Yosafat Donni Haryanto Yullita, Ilma Zulyani Zulyani