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Kajian Kualitas Mikrobiologi Permen Labu Kuning berdasarkan Lama Penyimpanan dan Macam Pembungkus Ditinjau dari Angka Lempeng Total Koloni Kapang Linda Hapsari; Utami Sri Hastuti; Sitoresmi Prabaningtyas
Jurnal Ilmu Hayat Vol 2, No 2 (2018)
Publisher : Universitas Negeri Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17977/um061v2i22018p61-73

Abstract

Permen labu kuning adalah produk olahan buah labu kuning yang merupakan makanan khas dari Sumbawa Besar. Dalam permen labu kuning terkandung senyawa-senyawa yang dapat dimanfaatkan oleh kapang kontaminan untuk tumbuh dan berkembang biak. Hal ini dapat menyebabkan penurunan kualitas permen sehingga mempengaruhi daya tahan simpannya. Tujuan penelitian ini adalah: 1)Mengungkap pengaruh lama waktu penyimpanan, macam pembungkus, dan interaksi lama waktu penyimpanan dan macam pembungkus terhadap kualitas mikrobiologi permen labu berdasarkan ALT koloni kapang. 2) Menentukan batas waktu simpan maksimal permen labu ditinjau berdasarkan ALT koloni kapang yang masih memenuhi syarat batas maksimal dari DIRJEN POM. Jenis penelitian ini adalah eksperimen dengan menggunakan Rancangan Acak Lengkap (RAL) Faktorial yang terdiri dari dua faktor yaitu lama waktu penyimpanan dan macam pembungkus. Sampel permen labu kuning sebanyak 25 gram dihaluskan, kemudian dilarutkan dalam 225 ml larutan air pepton 0,1%, kemudian diencerkan sampai tingkat pengenceran10-6. Inokulasi pada medium lempeng Czapek Agar (CA) masing-masing 0,1 ml dari setiap tingkat pengenceran. Selanjutnya dilakukan penghitungan ALT koloni kapang baik pada sampel yang dibungkus kertas minyak maupun plastik yang telah disimpan selama 0 hari, 2 hari, 4 hari, 6 hari , dan 8 hari,  kemudian dirujukkan pada ketetapan dari DIRJEN POM. Hasil penelitian ini menunjukkan bahwa 1) Lama penyimpanan berpengaruh signifikan terhadap ALT koloni kapang, 2) Macam pembungkus dan interaksi antara lama penyimpanan dan macam pembungkus tidak berpengaruh terhadap ALT koloni kapang, 3)Batas waktu simpan pada permen labu kuning yang dibungkus kertas minyak maksimal adalah 4 hari sedangkan yang dibungkus plastik maksimal adalah 6 hari.
The Result of Second Field Test of Cellulose Degrading Bacteria from Mangrove Soil Microbiology Handout Nugraheni, Febriani Sarwendah Asri; Hastuti, Utami Sri; Rohman, Fatchur
Journal of Biological Science and Education Vol 2, No 1 (2020): June
Publisher : Universitas Sembilanbelas November Kolaka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31327/jbse.v2i1.1227

Abstract

Learning media is one of the most important component in a learning process, both inside or outside a classroom. In order to provide proper learning for students based on the researcher’s prior research, at least, three different field tests are needed. This research is a quasi-experiment, which is provide result of the second field test of cellulose degrading bacteria from mangrove soil microbiology handout both in cognitive and psychomotor ability. The research aims are to describe effect of this microbiology handout on student’s cognitive achievement and gain data about inappropriate laboratory written instruction in this handout. Data gained by conducting learning activities using developed handout which were 2 times in class theory and three times of practical activity using instuctions written in the handout. In the end of class theory activity, students did a posttest to confirm their improvement of cognitive ability while their practical ability was observed using observation sheet along with their activities. Results showed that handout which being tested could improve student cognitive at average level (n gain 0,419). In contrast, several parts of laboratory written instruction need to be fixed to make better microbiology handout. 
Pengemasan dan pemasaran kopi biji salak (Salacca zalaca) melalui e-commerce untuk peningkatan perekonomian masyarakat desa Utomo, Muhammad Andry Prio; Rakhmawati, Yunita; Daniarsih, Ajeng; Hastuti, Utami Sri; Rozana, Kennis; Zahida, Nadila Sekar; Izzalqurny, Tomy Rizky
Jurnal Inovasi Hasil Pengabdian Masyarakat (JIPEMAS) Vol 7 No 2 (2024)
Publisher : University of Islam Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33474/jipemas.v7i2.20872

Abstract

Tingginya produksi keripik salak Monik (Salacca zalacca) di desa Tirtoyudo juga diimbangi dengan meningkatnya jumlah limbah biji salak yang dihasilkan. Biji salak umumnya dibuang karena keras dan sulit untuk didegradasi sehingga mengakibatkan pencemaran lingkungan. Tujuan dari pengabdian kepada masyarakat ini adalah untuk memberikan pelatihan tentang pengemasan produk kopi biji salak Monik dengan easy drip dan pemasarannya melalui e-commerce kepada ibu-ibu PKK Desa Tirtoyudo, Malang. Metode yang digunakan dalam pelatihan ini yaitu service learning, sehingga peserta dapat mencoba praktik mengemas produk kopi biji salak dan mengelola tokodi e-commerce setelah materi. Jumlah peserta pada kegiatan pelatihan ini adalah 40 orang. Hasil pelatihan ini adalah produk box kopi biji salak kemasan easy drip dalam box yang menarik dan toko online di Shopee dengan nama Zacco Official Store. Shopee dipilih sebagai e-commerce untuk memasarkan produk karena 50% peserta pelatihan pernah melakukan transaksi di Shopee. Selain itu pelatihan ini mampu meningkatkan pengetahuan peserta tentang e-commerce, perkembangan e-commerce di Indonesia dan manfaat e-commerce menjadi 100%. Hasil survey pada akhir pelatihan menunjukkan peserta menilai puas hingga sangat puas untuk pelaksanaan pelatihan ini. Tindak lanjut yang diharapkan dari pengabdian ini adalah produk kopi biji salak Monik dapat teregistrasi di BPOM dan Halal MUI sehingga kualitas produk terjamin.
Potential And Mechanism Antagonistic of Trichoderma sp. Againts Colletotrichum gloeosporioides and C. aotearoa on Avocado Plant (Persea americana Mill) Mafluhah, Luluk Rofiatul; Hastuti, Utami Sri; Prabaningtyas, Sitoresmi
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 9, No 3: Jurnal Pembelajaran Dan Biologi Nukleus November 2023
Publisher : Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v9i3.4689

Abstract

The objective of this study is to ascertain the pathogenic and antagonistic fungi that induce disease in avocado plants (Persea Americana Mill), identify the antagonistic fungi with the greatest antagonism potential among a variety of antagonistic fungi, and elucidate the mechanism by which antagonistic fungi inhibit the growth of pathogenic fungi. From January to May 2023, this study was conducted in vitro at the Microbiology Laboratory, State University of Malang, employing a completely randomized design (CRD) comprising six replications and four interventions. The identification of mold was accomplished through macroscopically and microscopically descriptive analysis, followed by a comparison with the mold identification key book. The antagonism process was executed utilizing the dual culture method on PDA medium, and the electron microscope (SEM) was employed to witness the antagonism mechanism. The research findings revealed that T. harzianum spp. and T. viride were identified as antagonist fungi, whereas C. aotearoa and C. gloeospoiroides were identified as pathogenic fungi. The antagonistic fungus T. viride exhibited the greatest degree of antagonism, impeding the growth of C. gloeospoioroides by 77.8%. Observed antagonism occurs when the hyphae of the antagonistic mold ensnare, entangle, and pierce those of the pathogenic mold, causing harm to the region surrounding the pathogenic mold's hyphae and subsequently impeding its growth
Development of Microbiology Module “Antibacterial Effect of Ginger Rhizome Extract to Inhibit the Growth of Propionibacterium acnes Bacteria” as a Teaching Material to Improve Students’ Problem Solving Skill Mahesti, Tika; Hastuti, Utami Sri; Sari, Murni Sapta
BIOEDUKASI Vol 22 No. 3 (2024)
Publisher : PROGRAM STUDI PENDIDIKAN BIOLOGI FAKULTAS KEGURUAN DAN ILMU PENDIDIKAN UNIVERSITAS JEMBER

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/bioedu.v22i3.47971

Abstract

This research is a research and development of module on the topic "Antibacterial Effect of Ginger Rhizome Extract to Inhibit the Growth of P. acnes Bacteria" as a teaching material in the learning process to improve students' problem solving skills. The aim of developing the module is to produce a valid, practical and effective teaching materials. The development of module used ADDIE model. Validation of the Microbiology Module was conducted by Microbiology material expert, learning media and teaching material experts, and Microbiology learning practitioner expert. The effectiveness of Microbiology Module in improving problem solving skills can be known through pretest and posttest scores analyzed through One Way Ancova test. The research and development results show that validation scores by learning media and teaching materials experts were 99.72%, Microbiology material experts were 87.56%, biology education practitioner experts were 99.33%, student practical response results were 87.05%. The efficiency results were 0.00 < 0.05 so it proved that the use of module is effective in the learning process, it can be concluded that the Microbiology Module category as very valid, very practical and effective as teaching material, so it can be used by students in Microbiology courses especially in improving problem solving skills.
A Microbiology Module Development on Antagonism Between Fungi and Bacteria Based on Problem-Based Learning to Improve Student’s Critical Thinking Skills Maknuna, Durrotul; Hastuti, Utami Sri; Prabaningtyas, Sitoresmi
BIOEDUKASI Vol 22 No. 3 (2024)
Publisher : PROGRAM STUDI PENDIDIKAN BIOLOGI FAKULTAS KEGURUAN DAN ILMU PENDIDIKAN UNIVERSITAS JEMBER

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/bioedu.v22i3.50920

Abstract

The purpose of the research is to develop Microbiology Module on the topic "The Antagonism of P. chrysogenum against Escherichia coli, Staphylococcus aureus, and Bacillus subtilis" that have criteria valid, practice, and effective for improve the student’s critical thinking skills. This research type is a quasi-experiment research using a non-randomized pretest-posttest control group design. The learning model uses the ADDIE approach which is implemented with the Problem Based Learning (PBL) model. Data collection uses response questionnaires, pretest and post-test, and validation sheets. The effectiveness of the Microbiology Module in improving students' critical thinking skills can be known through the pretest and post-test scores analyzed through the One Way Ancova test. The research and development result proved that the validation score gives by media and teaching materials experts was 99%, material experts were 98%, and Biology education practitioners were 100% from the student responses were 93%. The p-level results showed 0.00<0.05, so the modules were effective to use in the learning process. The research results proved that a significant difference between the experimental class learned using the Microbiology Module compared to the control class PowerPoint without the Microbiology Module. The modules is valid, practical, and effective to use in improve the student’s critical thinking skills.
Development of Microbiology Handout as Teaching Material to Improve Science Process Skills of Health Vocational Students Pratama, Aris Yudha; Hastuti, Utami Sri; Setiowati, Frida Kunti
BIOEDUKASI Vol 22 No. 3 (2024)
Publisher : PROGRAM STUDI PENDIDIKAN BIOLOGI FAKULTAS KEGURUAN DAN ILMU PENDIDIKAN UNIVERSITAS JEMBER

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/bioedu.v22i3.49473

Abstract

This research is a research and development of Microbiology Handout on the topic "Antibacterial Test on Bacterial Growth Inhibition" which uses natural materials, namely star fruit extract (Averrhoa carambola L.), which will be used as teaching materials in the learning process to help improve students' science process skills. The purpose of developing Microbiology Handout is to produce teaching materials that are valid, practical and effective for use in learning Microbiology subjects. Microbiology Handout development uses the ADDIE development model. Validation of Microbiology Handouts was carried out by learning media and teaching material expert validators, Microbiology material experts, and Microbiology learning practitioner expert validators. The Microbiology Handout trial was conducted through three stages of trials, namely a one-to-one trial of 3 students, a small group trial of 10 students, and a field trial of 15 students. The effectiveness of Microbiology Handout in improving students' science process skills can be seen through pretest and posttest scores analyzed using the IBM SPSS version 26 application through the One Way Ancova test. The results of research and development show the acquisition of validation scores by learning media and teaching material experts of 100%, Microbiology material experts of 94.3%, education practitioner experts of 99.64%, student response results of 91.43% and efficiency results of 0.028
Microbiology literacy and its influence on knowledge, perceptions and community attitudes Mustofa, Ali; Hastuti, Utami Sri; Susanto, Hendra
Biosfer: Jurnal Pendidikan Biologi Vol. 18 No. 1 (2025): Biosfer: Jurnal Pendidikan Biologi
Publisher : Universitas Negeri Jakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21009/biosferjpb.49273

Abstract

Microbiology literacy development shapes people's attitudes, behaviors, and perceptions toward microorganisms. This study aims to explore the relationship between microbiological literacy and people's perceptions and attitudes towards microorganisms. This study uses the Systematic Literature Review (SLR) method to analyze the relationship between microbiological literacy and public knowledge, attitudes, and perceptions of microorganisms. Data were retrieved from Scopus using relevant keywords and then filtered based on the following criteria: published between 2019–2024, in English, and open access. The analysis was conducted through four steps: coding, concept review, and summarizing key information (author, year, and core concepts). The results were synthesized to map the relationship between microbiological literacy and public perceptions. The results showed that respondents' knowledge levels varied about various health issues. Good microbiological knowledge can improve health literacy, affecting individual attitudes and perceptions of health. Regarding respondents' attitudes, it is known that respondents are interested in education and health awareness. A positive attitude toward health can encourage actions that support public health. In contrast, individuals with low microbiology knowledge orientation are more likely to cause negative perceptions and distrust of health interventions. Continuous, evidence-based education is essential to improve microbiology and health literacy and shape positive attitudes toward health interventions. Microbiology knowledge has a broad impact on daily life and the future. However, there are significant challenges in understanding microbiology. Public perception of microbes is often only associated with 'germs' and negative images. Therefore, microbiology knowledge in learning must be presented in visual arts and visual, olfactory, and tactile experiences. The more we understand the role and knowledge of microbes, the better we can make relevant decisions.
Active Compounds and Antibacterial Activity of Banana (Musa paradisiaca) var. Agung Bunches and Combs Extracts Against Escherichia coli Analysis Pratama, Ade Wahyu; Hastuti, Utami Sri
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 11, No 2: Jurnal Pembelajaran Dan Biologi Nukleus June 2025
Publisher : Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v11i2.7186

Abstract

Background: Escherichia coli cause diarrhea. E. coli infections can be treated using synthetic antibiotics. Natural antibiotics are an alternative for prevent E. coli infections as synthetyc antibiotic substitute. Natural antibiotics are come from plants part such as agung banana’s (Musa paradisiaca) bunch and combs. This bananas are used as a dessert fruits. The waste of this friut, such as bunches and fruit comb. This banana plant is potentially as antibacterial substance. The aims of this research are, 1) to examine the antibacterial effect of banana bunch and comb extracts; 2) to determine the effective concentration of the extract against the E.coli growth; and 3) to analysis of the active compounds content of banana bunch and comb extracts. Methodology: The antibacterial effect was examine using the agar well diffusion. Total active compound levels was examine using the UV-Vis spectrophotometry method and the derivative compounds uses the LCMS method. Findings: The research results showed the bunches had antibacterial effect against E. coli and the effective concentration is 90% with the highest inhibitory zone size diameter is 6.98 ± 0,00 mm, while the combs effective concentration is 80% is 6.55 ± 0.81 mm. The antibacterial effect of both extracts is moderate categorize. The highest content of active compounds in the bunches is phenol, with content: 70.70 mg/g and in the comb is 45.82 mg/g. Contribution: This research contributes to the utilisation of organic waste as an alternative source of environmentally friendly natural antibacterial agents and supports innovation in phytopharmaceuticals based on local plants.
Endophytic Fungi as A Symbion in Polianthes tuberosa L.: Identification, Microscopic Observation, and Secondary Metabolite Analysis Hastuti, Utami Sri; Abdini, Aulia; Puspitasari, Dela Reni; Hamidah, Rodiah Amin; Labibah, Sylvana Bilqis
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 11, No 3: Jurnal Pembelajaran Dan Biologi Nukleus September 2025
Publisher : Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v11i3.7928

Abstract

Background: Polianthes  tuberosa L. was known as an ornamental plants. People use this plant flower for ritual purpose because of the fragrant aroma. This plant rarely attacked by microorganism and usually stay healthy. Some endophytic fungi species live with P. tuberosaL. plant in mutualistic symbiosis interaction. This study was conducted to: (1) determine where the hyphae of endophytic fungi are located within the tissue of P. tuberosa L. plant petals and leaves, using findings from microscopic observations; (2) identify the species of endophytic fungi residing in the flower petals and leaves of P. tuberosa L.; and (3) ascertain the various secondary metabolites produced by each species of endophytic fungi. Methodology: P. tuberosa plant were obtained from flower market at Malang city. The leaves and flower petal parts were prepared for microscopic observation and identification. Cultures of each endophytic fungal species were grown on PDA plate medium, then cut into pieces measuring 5 x 1 cm and inoculated in PDB medium. These were shaken at a speed of 120 rpm for 7 days. After that, the liquid culture was centrifuged at 3000 rpm for 10 minutes. The contents of several secondary metabolites uses the supernatant of the liquid culture. The phytochemical analysis used spectrophotometry method.  Findings: The study findings are: (1) the endophytic fungi hyphae are located in stomata neighbor cells, the walls of xylem cells, epidermal cell walls, sponsa cell walls, and also within the petal epidermis cell walls of the flower; (2) eight endophytic fungi species have been identified; (3) every species of endophytic fungi generates flavonoids, alkaloids, tannins, saponins, and steroids, each with varying content levels. Contribution: Based on this research result, the endophytic fungi culture can be utilized as natural antibiotic source for better synthetic substitution. Besides that endophytic fungi also can be applied as antagonistic fungi to inhibite pathogenic fungi growth that attack some cultivated plant through appropriate research
Co-Authors Abdini, Aulia Abdul Fattah Noor Abdul Ghofur Abdul Gofur Abdul Gofur Abdul Gofur Abdul Gofur Agung Witjoro Ahmad Najib Ajeng Daniarsih Ali Mustofa Ana Syarifatun Nisa Anggia Oktantia Anindya Nirmala Permata Arif Rahman Arini Zahrotun Nasichah Ary Maf’ula Asna, Putri Moortiyani Al Aswal Salewangeng Aziz Tanama Balqis Balqis Betty Lukiati Chandra Kirana Chandra Kirana Chandra Kirana Choirunnisa, Hesti Nur Chomisatut Thoyibah Dina Istia’nah Dwi Listyorini Dwi Rahmawati Dwi Rahmawati Dwi Rakhmawati Dwi Rakhmawati Endang Suarsini Erna Wijayanti Faiza Nur Imawati Ningsih Fatchur Rohman Fatchur Rohman Fatchur Rohman Febriani Sarwendah Asri Nugraheni Febriani Sarwendah Asri Nugraheni Fitri, Rizka Diah Fitria Maulita Fitria Maulita Frida Kunti Setiowati Galina Istiqhfarini Gani, Abdul Rasyid Fakhrun Hadi Suwono Hamidah, Rodiah Amin Hanzen, W.F Edi Hendra Susanto Henny Nurul Khasanah Henny Nurul Khasanah Henny Nurul Khasanah Herawati Susilo Herdina Rahma Zalita Hesti Nur Choirunnisa Ibrohim Imam Mudakir Indah Sari Dewi Indriana Rahmawati Indriana Rahmawati Indriana Rahmawati Intan Rezki Kurniasari Istamar Syamsuri Izzalqurny, Tomy Rizky Izzatinnisa’ Izzatinnisa’ Khusnul Khotimah Kuniasari, Intan Rezki La Arlan Labibah, Sylvana Bilqis Lely Hermawati Linda Hapsari Linda Hapsari M. Ainur Yaqin M. Amin M. Nidhamul Maulana Mafluhah, Luluk Rofiatul Mahesti, Tika Maknuna, Durrotul Mareta Arisswara Edy Mariyanti Mariyanti Mashuri Saputra Mastika, Laily Maghfiro Kamil Maya Firdausi Prayudhani Muhammad Andry Prio Utomo Muhammad Syamsussabri Murni Sapta Sari Natalia Rosa Keliat Naufal Wima Al Fahri Ningsih, Husdiani Nugraheni, Febriani Sarwendah Asri Nursyahbani Saraha Nurul Yanuarsih Nuzalifa, Yossie Ulfa Otavia Dewi Permata Ika Hidayati Pratama, Ade Wahyu Pratama, Aris Yudha Puspitasari, Dela Reni Putri M. Al Asna Putri Moortiyani Al Asna Qorry Aulya Rohmana Rahel Natalia Saragih Munthe Rahmadyah Kusuma Putri Ria Yustika Sari Ria Yustika Sari Ria Yustika Sari Rina Kristina Maria Rizky, Mirza Yanuar Rofiqoh Lailatul Fitriyah Rozana, Kennis Safrudin M. Abidin Saidil Mursali Saidil Mursali Sari, Ria Yustika Siti Aisaroh Siti Annisaa&#039;ul Kariimah Siti Hartina Pratiwi Siti Zubaidah Siti Zubaidah Sitoresmi Prabaningtyas Sri Rahayu Lestari Sueb Sugeng Handiyanto Sugi Hartono Suhadi Suhadi Sulisetijono Sulisetijono Sundari, Syifa Syafitri, Nur Laila Umi Lestari Umu Fatonatul Hidayah W. F. Edi Hanzen W.F Edi Hanzen Wa Ode Nurhawa Yahmi Ira Setyaningrum Yessi Hermawati Yesy Maulina Nadhifah Yheni Sapitri Yulia Venicreata Dipu Yulia Venicreata Dipu Yunita Putri Irsadul Ummah Yunita Rakhmawati Zahida, Nadila Sekar