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Aulia Puspita Anugra Yekti
Dosen Produksi Ternak Fakultas Peternakan, Universitas Brawijay
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Social Sciences Veterinary Other

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Karakter Motilitas Spermatozoa Hasil Sexing pada Sapi Peranakan Ongole dengan Volume Awal yang Berbeda Rifai Mustofa; Aulia Puspita Anugra Yekti; Aryogi Aryogi; Dicky Pamungkas; Rizki Prafitri; Asri Nurul Huda; Kuswati Kuswati; Trinil Susilawati
Jurnal Agripet Vol 20, No 2 (2020): Volume 20, No. 2, Oktober 2020
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v20i2.16932

Abstract

ABSTRAK. Sexing spermatozoa bertujuan untuk mengatur jenis kelamin sesuai harapan. Salah satu metode sexing adalah dengan menggunakan Sentrifugasi Gradien Densitas Percoll. Tujuan dari penelitian ini adalah untuk mengetahui berbagai parameter motilitas menggunakan Computer-assisted Sperm Analysis dan proporsi spermatozoa X dan Y menggunakan metode Sentrifugasi Gradien Densitas Percoll dengan volume awal yang berbeda. Penelitian dilakukan di Loka Penelitian Sapi Potong Grati, Pasuruan, pada bulan Januari sampai Maret 2020. Materi yang digunakan adalah Semen Sapi Peranakan Ongole berumur 5 tahun dan bobot badan 700 kg sebanyak 3 ekor, motilitas masa 2+ dan motilitas individu 70%. Metode yang digunakan adalah eksperimental menggunakan Rancangan Acak Kelompok (RAK) dengan 3 perlakuan volume saat sexing yaitu P1= 1 ml, P2= 1,5 ml dan P3= 2 ml dengan ulangan 11 kali. Ulangan juga berfungsi sebagai kelompok (block). Hasil penelitian menunjukkan bahwa setelah dilakukan sexing dan pendinginan hingga 5oC persentase motilitas adalah P1:71,02+10,08 %; P2: 79,63+8,65 % dan P3: 83,38+6,67 %, sedangkan motilitas progresif pada P1: 47,68+8,71%; P2: 59,61+7,26 % dan P3: 62,21+6,74%. Curvilinear Velocity (VCL) pada P1: 50,97,73 m/s, P2: 55,25,03 m/s dan P3: 53,25,97%. Straight-line Velocity (VSL) pada P1: 20,88,19 m/s; P2: 21,66,02 m/s; P3: 22,15,77 m/s, sedangkan Average Path Velocity (VAP) pada P1: 30,27,82 m/s; P2: 32,56,14 m/s dan P3 : 31,56,18 m/s. Linearity (LIN) pada P1: 40,212,26%;P2: 39,110,31%;P3: 39,87,25%. Straightness (STR) pada P1: 67,211,20%; P2: 65,710,06%;P3: 67,27,92% sedangkan Wobble (WOB) pada P1: 599,49%; P2: 58,88,63% dan P3: 59,17,74%. Kesimpulan dari penelitian adalah motilitas dan motilitas progresif spermatozoa pada sampel dengan volume awal 2 ml lebih baik dibandingkan dengan sampel dengan volume awal 1 ml dan 1,5 ml.(Characteristics of Sexing Spermatozoa Motility in Ongole Cattle with Different Initial Volume) ABSTRACT. Sperm sexing is a technique of sorting a specific type of sperm cell to fertilize the egg cell. One of the sexing methods that can be used for spermatozoa sexing is percoll gradient density centrifugation. This research aims to find out various motility parameters using Computer-assisted Sperm Analysis and the proportion of spermatozoa X and Y using density gradient centrifugation method percoll with different initial volume. The study was conducted at Grati Beef Cattle Research Station, Pasuruan, from January to March 2020. Materials of the study were Semen of 3 Filial Ongole Cattles, aged 5 years, with 700 kg body weight, mass motility 2+, and individual motility 70%. The method used was experimental using a Randomized Group Design with 3 treatment volume when sexing i.e. T0= 1 ml, T1= 1.5 ml dan T2= 2 ml with repetition 11 times. The repetition also functions as groups (blocks). The results showed that after sexing and cooling up to 5oC, the percentage of motility is T0:71.02+10.08 %; T1: 79.63+8,65 % and T2: 83.38+6.67 %. Progressive motility on T0: 47.68+8.71%; T1: 59.61+7.26 % and T2: 62.21+6.74%. Curvilinear Velocity (VCL) on T0: 50.97.73 m/s, T1: 55.25.03 m/s and T2: 53.25.97%. Straight-line Velocity (VSL) on T0: 20.88.19 m/s; T1: 21.66.02 m/s; T2: 22.15.77 m/s. The Average Path Velocity (VAP) on T0: 30.27.82 m/s; T1: 32.56.14 m/s and T2 : 31.56.18 m/s. Linearity (LIN) on T0: 40.212.26%; T1: 39.110.31%;T2: 39.87.25%. Straightness (STR) on T0: 67.211.20%; T1: 65.710.06%; T2: 67.27.92%. Wobble (WOB) on T0: 599.49%; T1: 58.88.63% and T2: 59.17.74%. The conclusion of the study is the motility and progressive motility of spermatozoa in samples with an initial volume of 2 ml is better than samples with an initial volume of 1 ml and 1.5 ml.
Tingkat Keberhasilan Inseminasi Buatan Menggunakan Semen Beku Sexing pada Bangsa Sapi yang Berbeda Wiranto Wiranto; Kuswati Kuswati; Rizki Prafitri; Asri Nurul Huda; Aulia Puspita Anugra Yekti; Trinil Susilawati
Jurnal Agripet Vol 20, No 1 (2020): Volume 20, No. 1, April 2020
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v20i1.15811

Abstract

ABSTRAK. Penelitian ini bertujuan mengevaluasi tingkat keberhasilan IB semen beku sexing Y. Penelitian menggunakan 39 sapi Peranakan Ongole dan 77 sapi Persilangan Limousin yang di IB secara double dosis. IB menggunakan semen beku spermatozoa Y dengan metode pemisahan Sentrifugasi Gradien Densitas Percoll (SGDP) sapi Limousin yang diproduksi oleh Balai Besar Inseminasi Buatan (BBIB) Singosari. Teknik deposisi semen adalah 4+ yaitu pada posisi cornua uteri selanjutnya dilakukan IB pada jam ke 2 dan ke 8. Untuk akseptor dilakukan penyuntikan BioATP+ dan pemberian pakan konsentrat 3 kg per hari selama 3 hari setelah IB. Variabel penelitian meliputi Non Return Rate (NRR), Service Per Conception (S/C), dan Conception Rate (CR). Hasil penelitian menunjukkan bahwa IB sapi Peranakan Ongole dan sapi Persilangan Limousin yaitu NRR1 sebesar 82,05% dan 89,61%, nilai NRR2 sebesar 76,92% dan 84,42%, nilai CR sebesar 58,97% dan 74,03%, serta nilai S/C sebesar 1,78% dan 1,46%. Kesimpulan penelitian adalah IB menggunakan semen beku hasil sexing Y sapi Limousin pada sapi persilangan Limousin memiliki tingkat keberhasilan yang lebih tinggi dibandingkan sapi Peranakan Ongole.(The success rate of artificial insemination using frozen sexing semen of different breed cow)ABSTRACT. This study aimed to evaluate the success rate of AI frozen semen sexing Y. The material in this study used 39 Filial Ongole and 77 Limousin crossbred cattle in AI in double dose. The spermatozoa used were Y frozen semen of Limousin bull with the Percoll Gradient Density Centrifugation (SGDP) separation method produced by the Singosari Center for Artificial Insemination. The semen deposition technique was 4+, which has the position of the cornua uteri (deep Insemination), then AI implemented with a double dose at the 2nd and 8th hours after estrous. The cow acceptor was injected with BioATP+ and feed by 3 kg concentrate per day for three days after AI. Research variables include Non-Return Rate (NRR), Service Per Conception (S/C), and Conception Rate (CR). The results showed that AI of Peranakan Ongol and Limousin crossbred cattle on NRR1, NRR2, CR values, and S/C values respectively was 82.05% and 89.61%; 76.92% and 84.42%; 58.97% and 74.03%; 1.78% and 1.46%. In conclusion, AI using frozen semen from sexing Y Limousin cattle in Limousin crossbred cows had a higher success rate than Filial Ongole cattle.
Hubungan antara Tingkah Laku Seksual dengan Produksi Spermatozoa Sapi Brahman Nisa'us Sholikah; Aris Sutomo; Ndaru Pranowo Widiasmoro; Sri Wahjuningsih; Aulia Puspita Anugra Yekti; Kuswati Kuswati; Trinil Susilawati
Jurnal Agripet Vol 18, No 2 (2018): Volume 18, No. 2, Oktober 2018
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v18i2.12036

Abstract

ABSTRAK. Pejantan untuk program inseminasi buatan adalah pejantan unggul yang diseleksi berdasarkan libido dan produksi spermatozoa. Penelitian bertujuan untuk menganalisa hubungan antara tingkah laku seksual dengan produksi spermatozoa sapi Brahman. Materi penelitian adalah 3 pejantan sapi Brahman yang ditampung menggunakan vagina buatan. Metode penelitian adalah observasional dengan mengamati waktu reaksi dan lama ejakulasi. Observasi laboratorium dengan mengamati volume, konsentrasi, dan motilitas individu untuk menghitung total spermatozoa (TS) dan total spermatozoa motil (TSM). Pengamatan dilakukan sebanyak 10 ulangan. Data dianalisa secara deskriptif serta hubungan antara tingkah laku seksual dan produksi spermatozoa dihitung dengan korelasi Pearson. Hasil penelitian menunjukkan terdapat variasi tingkah laku seksual dan produksi spermatozoa pada sapi Brahman. Individu Cipta mempunyai waktu reaksi dan lama ejakulasi paling singkat serta produksi spermatozoa paling rendah. Hubungan signifikan antara waktu reaksi dengan TS serta TSM pada individu Bizzyard dengan koefisien determinasi (R2) sebesar 14 % dan 20%. Hubungan sangat signifikan antara lama ejakulasi dengan TS serta TSM pada individu Cipta dengan R2 sebesar 46% dan 58%. Kesimpulan penelitian adalah terdapat hubungan antara tingkah laku seksual dengan produksi spermatozoa pada sapi Brahman, dengan koefisien korelasi (r) tertinggi sebesar 0,72 dan R2 sebesar 58% antara lama ejakulasi dan total spermatozoa motil pada individu Cipta.(The relationship between sexual behavior and semen production in Brahman bulls)ABSTRACT. Bulls for artificial insemination must be selected based on libido and sperm production. The study aimed to analyze the relationship between sexual behavior with sperm production on Brahman bulls. The material was 3 Brahman bulls collected using artificial vagina. The method was observational by observing reaction time and ejaculation duration. Laboratory observations by observing volume, concentration, and motility to calculate total sperm (TS) and total motile sperm counts (TMSC). Observations were replicated 10 times. The data were analyzed descriptively, the relationship between behavior and sperm production was examined using Pearson correlation. The results showed that there were variations in sexual behavior and sperm production in each bull. Cipta have the shortest reaction time and ejaculation duration and the lowest sperm production. A significant relationship between reaction time and TS and TMSC in Bizzyard with coefficient of determination (R2) of 14% and 20%. A very significant between ejaculation duration with TS and TMSC in Cipta with R2 of 46% and 58%. In conclusion, there was a relationship between sexual behavior and sperm production in Brahman bulls, with the highest correlation coefficient (r) of 0.72 and R2 of 58% between ejaculation duration and the total motile sperm counts in Cipta.
The success rate of artificial insemination in Limousin crossbred heifer with additional concentrate Putri Utami; Andriawan Soni Hantoro; Rizki Prafitri; Kuswati Kuswati; Asri Nurul Huda; Aulia Puspita Anugra Yekti; Trinil Susilawati
Livestock and Animal Research Vol 22, No 1 (2024): Livestock and Animal Research
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/lar.v22i1.67613

Abstract

Objective: This study aimed to determine whether the addition of concentrate to heifers could be the same as the success of AI in calving cows in terms of Return Rate (NRR), Conception Rate (CR), and Pregnancy Rate (PR).Methods: The material used in this study was 28 Limousine Crossbred cows with 13 cows and 15 heifers. There were two treatments, namely T0: Cows that had given birth with control feed (control without added concentrate) + BIO ATP after AI, while T1: Heifers with control feed and the addition of 1kg/day concentrate for seven days after AI + BIO ATP. Samples were selected by purposive sampling with the criteria of BCS 3-5 (1-9) and aged ≥ 2 years. The artificial insemination method uses rectovaginal with 4+ deposition at the 2nd and 8th hour after estrus, then injection of Bio ATP brand "Rheinbio" intramuscularly as much as 10 ml.Results: The results showed that the reproductive performance of Limousine Crossbred Cow that had given birth (T0) was NRR1 84.62%, NRR2 84.62%, CR 53.33%, and PR 66.67%, while in heifers (T1), it was NRR1 86 .67%, NRR2 60%, CR 20%, and PR 20%.Conclusions: The addition of concentrate feed of 1 kg/head/day for seven days before AI in heifers (T1) showed lower reproductive success than in cows that had given birth (T0) even without the addition of concentrate.
The Successful Artificial Insemination with Frozen Semen from Different Genetic Qualities of Friesian Holstein Bull Yekti, Aulia Puspita Anugra; Hasan, Ismail; Hermanto; Febrianto, Nanang; Isnaini, Nurul; Utami, Putri; Syah, Habib Asshidiq; Wahjuningsih , Sri; Susilawati, Trinil
Jurnal Ilmu-Ilmu Peternakan Vol. 34 No. 3 (2024): December 2024
Publisher : Faculty of Animal Science, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jiip.2024.034.03.15

Abstract

This study aimed to evaluate the success of artificial insemination (AI) using frozen semen with different genetic qualities of Friesian Holstein Bull. In this research, the bulls came from the artificial insemination center of Singosari, consisting of Friesian Holstein bulls with proven bull certificates and the pure breed from embryo transfer. The frozen semen was produced by standard semen processing in the artificial insemination center of Singosari. The inseminated female totals were 56 head of Friesian Holstein crossbred in Pujon district, Malang, Indonesia. The females were divided into two groups: 28 cows receiving insemination from frozen semen of proven bull (T1) and 28 from pure breed FH bull from embryo transfer (T2). The females were chosen with purposive sampling with the criteria, have a Body Condition Score (BCS) of at least 2.5 (scale 1–5), have normal reproductive organs, and exhibit the clear estrus sign. Artificial insemination was performed with a single dose at the eight hour after estrus. Semen deposition was at the corpus uteri, while the thawing process was performed at 22–23°C for 60 seconds. The result showed that the non-return rate 1 (NRR-1), non-return rate 2 (NRR-2), and conception rate (CR) values for AI using frozen semen from a proven bull were 96%, 75% and 43%, respectively. In contrast, the percentage of AI success using pure breed FH bull from embryo transfer was 93%, 86%, and 64%. In conclusion, AI success using pure breed FH bull from embryo transfer was better than proven bull frozen semen.
PELATIHAN PEMASARAN PRODUK MAGGOT MELALUI MARKETPLACE PADA KELOMPOK PETERNAK SAPI PERAH KECAMATAN PUJON KABUPATEN MALANG Isnaini, Nurul; Yekti, Aulia Puspita Anugra; Hamiyanti, Adelina Ari; Sulistyo, Hanief Eko; Andri, Faizal
Kumawula: Jurnal Pengabdian Kepada Masyarakat Vol 8, No 1 (2025): Kumawula: Jurnal Pengabdian Kepada Masyarakat
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/kumawula.v8i1.51103

Abstract

The management of biogas sludge waste from dairy farming operation has not been properly managed, potentially causing environmental pollution. Biogas sludge waste can be utilized as a medium for maggot cultivation, which will yield products such as maggot flour and organic fertilizer, thus realizing a circular economy in dairy farming. This community service program aims to enhance the knowledge and skills of the Dairy Farmer Group in the Pujon District, Malang Regency, in marketing maggot products through online marketplaces. This program was conducted from June to August 2023. The method used in this community service is Participatory Learning and Action, implemented through activities such as socialization, discussions, and hands-on practice. The topics covered during socialization and discussions include the utilization of online marketplaces for marketing maggot products. Participants then engage in hands-on activities, including creating accounts, filling in store profile information, selecting delivery services, filling in bank account details, and uploading products. Participants actively and enthusiastically took part in the program. The initial evaluation results before the program indicate that the knowledge and skills of participants related to the target marketing of maggot products, types of online marketplaces, and marketing management with online marketplaces, scored 80, 65, and 70, respectively. Subsequently, the evaluation after program implementation showed an improvement in knowledge and skills of participants related to target marketing of maggot products, types of online marketplaces, and marketing management with online marketplaces, with scores of 95, 100, and 90, respectively. In conclusion, this community service has successfully enhanced the knowledge and skills of the Dairy Farmer Group in the Pujon District, Malang Regency, in marketing maggot-based products through online marketplaces.Hingga saat ini limbah sludge biogas pada peternakan sapi perah belum dikelola dengan baik sehingga berpotensi menyebabkan pencemaran lingkungan. Limbah sludge biogas dapat dimanfaatkan sebagai media budidaya maggot yang akan menghasilkan produk berupa tepung maggot dan pupuk organik sehingga dapat mewujudkan ekonomi sirkular pada peternakan sapi perah. Tujuan pengabdian kepada masyarakat ini adalah untuk meningkatkan pengetahuan dan keterampilan Kelompok Peternak Sapi Perah Kecamatan Pujon Kabupaten Malang dalam melakukan pemasaran produk budidaya maggot melalui marketplace. Program ini dilaksanakan mulai bulan Juni hingga Agustus 2023. Metode yang digunakan pada pengabdian kepada masyarakat ini adalah Participatory Learning and Action yang dilaksanakan melalui kegiatan sosialisasi, diskusi, dan praktik. Materi yang disampaikan pada saat sosialisasi dan diskusi adalah tentang pemanfaatan marketplace untuk pemasaran produk budidaya maggot. Peserta kemudian melaksanakan praktik mulai dari pembuatan akun, pengisian informasi profil toko, penentuan jasa pengiriman, pengisian rekening bank, dan unggah produk. Peserta terlibat secara aktif dan antusias dalam pelaksanaan program ini. Hasil evaluasi awal menunjukkan bahwa pengetahuan dan keterampilan peserta terkait target pemasaran produk budidaya maggot, jenis marketplace, dan manajemen pemasaran dengan marketplace sebelum dilaksanakan program memiliki skor masing-masing sebesar 80, 65, dan 70. Selanjutnya, evaluasi setelah pelaksanaan program menunjukkan bahwa pengetahuan dan keterampilan peserta terkait target pemasaran produk budidaya maggot, jenis marketplace, dan manajemen pemasaran dengan marketplace mengalami peningkatan dengan skor masing-masing sebesar 95, 100, dan 90. Kesimpulan pengabdian kepada masyarakat ini adalah bahwa kegiatan yang telah dilaksanakan mampu meningkatkan pengetahuan dan keterampilan Kelompok Peternak Sapi Perah Kecamatan Pujon Kabupaten Malang dalam melakukan pemasaran produk budidaya maggot melalui marketplace.
The Quality of Sexed Bali Bull Semen Using Percoll Density Gradient Centrifugation in Various Gradients with Tris Aminomethane Diluent Rahmawati, Nanda Ayu; Yekti, Aulia Puspita Anugra; Wahyudi, Aditiya; Nafis, Fardha Ad Durrun; Isnaini, Nurul; Susilawati, Trinil
Jurnal Ilmu-Ilmu Peternakan Vol. 35 No. 1 (2025): April 2025
Publisher : Faculty of Animal Science, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jiip.2025.035.01.4

Abstract

This research aims to determine the quality of spermatozoa in the sexed semen that separated using the Percoll Density Gradient Centrifugation (PDGC) method with various gradients using egg yolk tris aminomethane diluent. The study was conducted at the Singosari Artificial Insemination Center from December 2023 to February 2024. The study's material was the semen of one Bali bull with individual motility ? 70%. The experimental design used a Randomized Group Design with the sexed semen PDGC method treatment: T1 = 10 gradients, T2 = 5 gradients, and T3 = 3 gradients, each with ten replications. The analysis showed that the gradient variations significantly impact the individual motility on the bottom layer of sexed semen (P<0.05). Conversely, gradient variance did not affect viability, abnormality, concentration, total motile spermatozoa, and individual motility on the top layer of sexed semen (P>0.05). In conclusion, sexed semen using the PDGC method with varied gradients is suitable for the freezing process due to its high quality.
Co-Authors Achadiah Rachmawati Adelina Ari Hamiyanti Adelina Ari Hamiyanti Ahmad Budi Purnawan Ahmad Salim Ali Mahfud Ali mahfud Alifian Ibnu Ansori Amir Firdaus Andriawan Soni Hantoro Anisa Ramadhani Ardyah Ramadhina Irsanti Putri Aris Sutomo Aryogi Aryogi Asri Nurul Huda Asri Nurul Huda Aziz, Achmad Fadhli Azna Roudlotul Nur Umamah Budi Hartono Chusnul Hanim Dandy Prasetyo Dandy Ulul Azmi Dedi Muhammad Deny Sulistyowati Dewi Masyithoh Dian Ratnawati Dicky Pamungkas Dwi Susan Setiyani Erin Ayu Octaviani Errico Errico Faizal Andri fakhri alfi annashru Febrianto, Nanang Firlia Safa Gatot Ciptadi Habib Asshidiq Syah Hanief Eko Sulistyo Hermanto Hisyam Ryandhika Kusuma Ika Fitri Puspitasari Ika Nurjannah Irvan Mardi Ismail Hasan, Ismail Jois Harsah Joko Riyanto Khairul Rasyad Kurniaesa, Titah Ulya Kusmartono Kusmartono Kuswati , Kuswati Kuswati Kuswati Kuswati Lukman Affandhy Sunarto Luqman Affandhy M. Nur Ihsan Mad Nasir Shamsudin Mashitah ShikhMaidin Mashudi , Mashudi Mashudi Moch Saifudin Muchamad Luthfi Muhammad Ade Salim Muhammad Agus Tahar Muhammad Azharil Faris Muhammad Bayuardhi Muhammad Dikman Muhammad Helmi Muhammad Nur Ihsan Muhammad Zainul Hanif Muhammad Zainul Hanif Nadya Meyta Andriani Nafis, Fardha Ad Durrun Nanang Febrianto Nanang Febrianto Nanang Febrianto Nareswara Hapsari Ndaru Pranowo Widiasmoro Nisa, Dhinda Choirun Nisa’us Sholikah Nolasco da costa Nursahida Ramadhani Nurul Isnaini Nurul Isnaini Nurul Layla Nuryadi Nuryadi Poespitasari Hazanah Ndaru Priyo Sugeng Winarto Puji Akhiroh Putri Utami Putri Utami Putri Utami Putri Utami Putri Utami, Putri Rahmawati, Nanda Ayu Ravenska Ravenska Rheta Eva Ramadhani Setiawan Rifai Mustofa Rizki Prafitri Rizki Prafitri Safitri, Brilla Ismaya Sitiadira Aulia Aldini Sri Wahjuningsih Sri Wahjuningsih Sulistyo, Hanief Eko Syah, Habib Asshidiq Trinil Susilawati Trinil Susilawati Trinil Susilawati Trinil Susilawati Trinil Susilawati Trinil Susilowati Wahjuningsih , Sri Wahyudi, Aditiya Willy Saputra Saputra Tatulus Wiranto Wiranto Yadi Malda