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Aulia Puspita Anugra Yekti
Dosen Produksi Ternak Fakultas Peternakan, Universitas Brawijay
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Social Sciences Veterinary Other

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Effect of Thawing Technique on the Quality and Status of Acrosome Reactions Safitri, Brilla Ismaya; Wahjuningsih, Sri; Yekti, Aulia Puspita Anugra; Susilawati, Trinil
Jurnal Ilmu dan Teknologi Peternakan Tropis Vol 9, No 2 (2022): JITRO, May
Publisher : Universitas Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (593.633 KB) | DOI: 10.33772/jitro.v9i2.23126

Abstract

The success of Artificial Insemination is affected by the thawing technique used by the inseminator. The difference in thawing technique can affect the quality of the spermatozoa produced. This study aims to determine the effect of temperature and duration of thawing on the frozen semen quality of Bali and Madura cattle. The material used was 80 frozen semen straws for Bali cattle and Madura cattle. The method used is an experiment with four treatments and 10 replications, that is thawing in water at 37℃ for 30 seconds duration, 28℃ for 30 seconds, 28℃ for 45 seconds and 28℃ for 60 seconds. The variables observed in this study were capacitation, intact acrosome, intact plasma membrane and chromatin damage.  The data were analyzed using a Randomized Block Design with replication as a block. The results of the analysis of variance showed that thawing technique had significant effect (p<0.05) on not yet capacitated, intact acrosome, intact plasma membrane.  Thawing technique had no significant effect (p>0.05) on the acrosome reacted and chromatin damage.Keywords:  thawing technique, capacitation, intact acrosome, intact plasma membrane, chromatin damage
Kualitas Semen Beku Sapi Limousin setelah Thawing Menggunakan Air Dingin dengan Lama Waktu yang Berbeda Aulia Puspita Anugra Yekti; Rheta Eva Ramadhani Setiawan; Achadiah Rachmawati; Trinil Susilawati
Jurnal Agripet Vol 23, No 1 (2023): Volume 23, No. 1, April 2023
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v23i1.23331

Abstract

ABSTRACT. Keberhasilan inseminasi buatan dipengaruhi oleh kualitas semen atau motilitas spermatozoa post thawing. Inseminator menggunakan air dingin (28oC) untuk thawing semen beku sedangkan SNI menyarankan thawing semen beku dengan air hangat (37oC) selama 30 detik. Tujuan dari penelitian ini adalah untuk mengetahui pengaruh lama dan suhu thawing pada semen beku sapi Limousin terhadap kualitas semen serta mengetahui perlakuan terbaik dari penelitian ini. Materi yang digunakan adalah 50 straw semen beku sapi Limousin yang diproduksi oleh BBIB Singosari. Metode penelitian yang digunakan adalah eksperimental dengan 5 perlakuan dan 10 ulangan. Variabel yang diamati adalah Motilitas Individu spermatozoa, Viabilitas spermatozoa, Abnormalitas spermatozoa, Konsentrasi dan Total Motilitas Spermatozoa. Data yang diperoleh dianalisis menggunakan analisis ragam Analysis of Variance (ANOVA) dengan Rancangan Acak Kelompok (RAK) kemudian diuji dengan Duncan Multiple Range Test (DMRT) jika terdapat perbedaan nyata. Selanjutnya diuji Chi square untuk variabel motilitas individu, konsentrasi dan total spermatozoa dan dibandingkan dengan SNI untuk semen beku. Dari hasil penelitian, didapatkan bahwa thawing pada suhu 28C dengan lama waktu 60 detik berpengaruh sangat nyata (P0,01) terhadap viabilitas dan berpengaruh nyata (P0,05) pada motilitas individu dan total spermatozoa motil. Sedangkan, konsentrasi dan abnormalitas spermatozoa memberikan hasil tidak berpengaruh nyata (P0,05). Kesimpulan dari penelitian ini adalah thawing dengan air dingin (suhu 28C) selama 60 detik memberikan kualitas semen terbaik setelah thawing.(Quality of Post Thawing Frozen Semen on Limousin Bull Using Cold Water with Different Durations)ABSTRAK. The quality of post-thawing semen influences the success factor of artificial insemination (AI). Inseminators usually used tap water (28C) to thaw the frozen semen, while Indonesian National Standard used warm water (37C) for 30 seconds. This study aims to determine the effect of thawing duration on the frozen semen quality of Limousin Bull and also to know the best treatment between the five treatments of this experiment. The material used in this study was 50 straws of frozen semen produced by Artificial Insemination Center Singosari. The method was an experiment method with five treatments and ten replications. the variables observed were individual motility, sperm viability, sperm abnormality, concentration, and total motile of sperm. The data obtained were analyzed statistically using Analysis of Variance (ANOVA) of Randomized Block Design (RBD) and then using Duncan Multiple Range Test if there is a difference. Then using Chi-Square Test for individual motility, concentration, and total motile of sperm and compared with the Indonesian National Standard of frozen semen. The results show that a temperature of 28C with a duration of 60 seconds is highly significant (P0,01) to viability and significant (P0,05) to individual motility and total motile of sperm. While, the concentration and abnormality of sperm give no significant effect (P0,05). In conclusion, the temperature and duration of thawing at 28C for 60 seconds show the best quality of post-thawing semen.
Evaluation The Success of Artificial Insemination Using Frozen Sexed Semen Based on Different Estrus Characters Putri Utami; Muhammad Zainul Hanif; Aulia Puspita Anugra Yekti; Rizki Prafitri; Asri Nurul Huda; Kuswati Kuswati; Kusmartono Kusmartono; Trinil Susilawati
Jurnal Agripet Vol 22, No 2 (2022): Volume 22, No. 2, Oktober 2022
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v22i2.23423

Abstract

ABSTRACT. Knowledge of farmers about estrus detection is one of the factors that affect fixed-time artificial insemination (AI). This study aims to evaluate the success rate of AI using Y sexing frozen semen based on the estrus character of cattle. Forty-five Limousin Crossed Cows involved in this research with a Body Condition Score of 3-5 (1-9 scale), 1.8-7 years old. Y sexing frozen semen is produced by the Singosari Center for Artificial Insemination using Percoll's Gradient Density Centrifugation method. Observation of estrus character was done before insemination with the deep insemination technique of AI. Artificial insemination was carried out using double doses at the 2nd and 8th hours after estrus. Rheinbio vitamins as BioATP+ are injected after AI. The results showed that the Conception Rate and Pregnancy Rate based on the estrus character was higher as indicated by the red colour of the vulva was 17.78% and 22.22%; very swollen vulva was 20% and 22.22%; abundant cervical mucus was 15.56% and 17.78%, and vaginal temperatures ranging from 38.0-38.5oC was 13.33% and 20%. In conclusion, the low percentage of pregnancy in this study was caused by various factors, mainly the genetic quality, early embryonic efficacy, maintenance management especially feed, and there were cows that experienced reproductive disorders.(Evaluasi keberhasilan inseminasi buatan menggunakan semen beku sexing berdasarkan karakter estrus yang berbeda)ABSTRAK. Pengetahuan peternak mengenai deteksi estrus merupakan salah satu faktor yang berpengaruh terhadap ketepatan waktu inseminasi. Penelitian ini bertujuan untuk mengevaluasi tingkat keberhasilan IB menggunakan semen sexing Y berdasarkan karakter estrus ternak. Akseptor yang digunakan pada penelitian ini berjumlah 45 ekor Sapi Persilangan Limousin dengan BCS 3-5 dan umur berkisar antara 1,8-7 tahun. Semen beku sexing Y merupakan hasil produksi Balai Besar Inseminasi Buatan (BBIB) Singosari menggunakan metode Sentrifugasi Densitas Gradien Percoll (SGDP). Pengamatan karakter estrus dilakukan sebelum inseminasi. Inseminasi Buatan dilakukan dengan menggunakan double dosis pada jam ke-2 dan jam ke-8 dengan teknik deep insemination. Vitamin Rheinbio sebagai BioATP+ diinjeksikan setelah IB. Hasil penelitian menunjukkan bahwa keberhasilan kebuntingan ditinjau dari nilai conception rate (CR) dan pregnancy rate (PR) berdasarkan karakter estrus ternak lebih tinggi ditunjukkan pada kondisi warna vulva merah merata yaitu 17,78% dan 22,22%; vulva yang sangat bengkak yaitu 20% dan 22,22%; banyak mengeluarkan lendir servik yaitu 15,56% dan 17,78%, serta suhu vagina yang berkisar antara 38,0-38,5oC yaitu 13,33% dan 20%. Persentase kebuntingan yang rendah pada penelitian ini disebabkan oleh berbagai faktor, utamanya kualitas genetik ternak, kematian embrio dini, manajemen pemeliharaan khususnya pakan, dan terdapat ternak yang mengalami gangguan reproduksi.
Perbedaan Keberhasilan Inseminasi Buatan Menggunakan Metode Dosis Tunggal dan Ganda pada Sapi Madura Khairul Rasyad; Aulia Puspita Anugra Yekti; Kuswati Kuswati; Trinil Susilawati
Jurnal Agripet Vol 22, No 2 (2022): Volume 22, No. 2, Oktober 2022
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v22i2.24532

Abstract

ABSTRACT. Penelitian ini bertujuan mengevaluasi keberhasilan IB menggunakan metode dosis tunggal dan ganda pada sapi Madura. Penelitian menggunakan 25 ekor dengan metode IB dosis tunggal dan 25 ekor dengan metode IB dosis ganda. IB menggunakan semen beku sapi Madura yang diproduksi oleh Balai Besar Inseminasi Buatan (BBIB) Singosari, Malang. Teknik deposisi semen adalah 4+ (deep insemination) yaitu pada posisi cornua uteri selanjutnya dilakukan IB dosis tunggal (jam ke-8 setelah menunjukkan tanda-tanda berahi) dan IB dosis ganda (jam ke-2 dan ke-8 setelah menunjukkan tanda-tanda berahi). Akseptor diinjeksi dengan Bio ATP+ merek Rheinbio 10 ml per ekor dan pemberian pakan konsentrat merek Novo NC62 1 kg per ekor per hari selama tujuh hari setelah IB. Variabel penelitian meliputi Non Return Rate (NRR), Conception Rate (CR), dan Service Per Conception (S/C). Hasil penelitian menunjukkan bahwa IB dosis tunggal menghasilkan nilai NRR 1 sebesar 92%, NRR 2 sebesar 80%, CR sebesar 60%, dan S/C sebesar 1,71. Sedangkan pada IB dosis ganda menghasilkan nilai NRR 1 sebesar 92%, NRR 2 sebesar 84%, CR sebesar 68%, dan S/C sebesar 1,53. Kesimpulan penelitian adalah nilai NRR, CR, dan S/C pada sapi Madura dengan IB dosis ganda lebih baik daripada dengan IB dosis tunggal, dan IB dosis ganda dapat meningkatkan conception rate pada sapi Madura.(The success different of artificial insemination using single and double dosage methodof Madura cows)ABSTRAK. This study aims to evaluate the success of AI using methods single and double dosage plus in Madura cows. The study used 25 Madura cow using the AI method single dosage and 25 Madura cow using the AI method double dosage. AI uses frozen semen from Madura cows produced by the Singosari Center for Artificial Insemination. The semen deposition technique is 4+ (deep insemination) in the cornua uteri position then AI implemented with a single dosage (8th hours after showing signs of estrus) and double dosage (2nd and 8th hours after showing signs of estrus). The acceptors were injected with 10 ml of "Rheinbio'' brand Bio ATP+ per cow and 1 kg of concentrate "Novo NC62" brand per cow per day was given for seven days after IB. Research variables include Non Return Rate (NRR), Conception Rate (CR), and Service Per Conception (S/C). The results showed that AI of single dosage had value NRR 1 of 92%, NRR 2 of 80%, CR of 60%, and S/C of 1.71. While AI of double dosage had value NRR 1 of 92%, NRR 2 of 84%, CR of 68%, and S/C of 1.53. In conclusion, the value of NRR, CR, and S/C in Madura cows with AI double dosage was better than AI single dosage, and AI double dosage could increase the conception rate in Madura cows.
Karakter Motilitas Spermatozoa Hasil Sexing pada Sapi Peranakan Ongole dengan Volume Awal yang Berbeda Rifai Mustofa; Aulia Puspita Anugra Yekti; Aryogi Aryogi; Dicky Pamungkas; Rizki Prafitri; Asri Nurul Huda; Kuswati Kuswati; Trinil Susilawati
Jurnal Agripet Vol 20, No 2 (2020): Volume 20, No. 2, Oktober 2020
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v20i2.16932

Abstract

ABSTRAK. Sexing spermatozoa bertujuan untuk mengatur jenis kelamin sesuai harapan. Salah satu metode sexing adalah dengan menggunakan Sentrifugasi Gradien Densitas Percoll. Tujuan dari penelitian ini adalah untuk mengetahui berbagai parameter motilitas menggunakan Computer-assisted Sperm Analysis dan proporsi spermatozoa X dan Y menggunakan metode Sentrifugasi Gradien Densitas Percoll dengan volume awal yang berbeda. Penelitian dilakukan di Loka Penelitian Sapi Potong Grati, Pasuruan, pada bulan Januari sampai Maret 2020. Materi yang digunakan adalah Semen Sapi Peranakan Ongole berumur 5 tahun dan bobot badan 700 kg sebanyak 3 ekor, motilitas masa 2+ dan motilitas individu 70%. Metode yang digunakan adalah eksperimental menggunakan Rancangan Acak Kelompok (RAK) dengan 3 perlakuan volume saat sexing yaitu P1= 1 ml, P2= 1,5 ml dan P3= 2 ml dengan ulangan 11 kali. Ulangan juga berfungsi sebagai kelompok (block). Hasil penelitian menunjukkan bahwa setelah dilakukan sexing dan pendinginan hingga 5oC persentase motilitas adalah P1:71,02+10,08 %; P2: 79,63+8,65 % dan P3: 83,38+6,67 %, sedangkan motilitas progresif pada P1: 47,68+8,71%; P2: 59,61+7,26 % dan P3: 62,21+6,74%. Curvilinear Velocity (VCL) pada P1: 50,97,73 m/s, P2: 55,25,03 m/s dan P3: 53,25,97%. Straight-line Velocity (VSL) pada P1: 20,88,19 m/s; P2: 21,66,02 m/s; P3: 22,15,77 m/s, sedangkan Average Path Velocity (VAP) pada P1: 30,27,82 m/s; P2: 32,56,14 m/s dan P3 : 31,56,18 m/s. Linearity (LIN) pada P1: 40,212,26%;P2: 39,110,31%;P3: 39,87,25%. Straightness (STR) pada P1: 67,211,20%; P2: 65,710,06%;P3: 67,27,92% sedangkan Wobble (WOB) pada P1: 599,49%; P2: 58,88,63% dan P3: 59,17,74%. Kesimpulan dari penelitian adalah motilitas dan motilitas progresif spermatozoa pada sampel dengan volume awal 2 ml lebih baik dibandingkan dengan sampel dengan volume awal 1 ml dan 1,5 ml.(Characteristics of Sexing Spermatozoa Motility in Ongole Cattle with Different Initial Volume) ABSTRACT. Sperm sexing is a technique of sorting a specific type of sperm cell to fertilize the egg cell. One of the sexing methods that can be used for spermatozoa sexing is percoll gradient density centrifugation. This research aims to find out various motility parameters using Computer-assisted Sperm Analysis and the proportion of spermatozoa X and Y using density gradient centrifugation method percoll with different initial volume. The study was conducted at Grati Beef Cattle Research Station, Pasuruan, from January to March 2020. Materials of the study were Semen of 3 Filial Ongole Cattles, aged 5 years, with 700 kg body weight, mass motility 2+, and individual motility 70%. The method used was experimental using a Randomized Group Design with 3 treatment volume when sexing i.e. T0= 1 ml, T1= 1.5 ml dan T2= 2 ml with repetition 11 times. The repetition also functions as groups (blocks). The results showed that after sexing and cooling up to 5oC, the percentage of motility is T0:71.02+10.08 %; T1: 79.63+8,65 % and T2: 83.38+6.67 %. Progressive motility on T0: 47.68+8.71%; T1: 59.61+7.26 % and T2: 62.21+6.74%. Curvilinear Velocity (VCL) on T0: 50.97.73 m/s, T1: 55.25.03 m/s and T2: 53.25.97%. Straight-line Velocity (VSL) on T0: 20.88.19 m/s; T1: 21.66.02 m/s; T2: 22.15.77 m/s. The Average Path Velocity (VAP) on T0: 30.27.82 m/s; T1: 32.56.14 m/s and T2 : 31.56.18 m/s. Linearity (LIN) on T0: 40.212.26%; T1: 39.110.31%;T2: 39.87.25%. Straightness (STR) on T0: 67.211.20%; T1: 65.710.06%; T2: 67.27.92%. Wobble (WOB) on T0: 599.49%; T1: 58.88.63% and T2: 59.17.74%. The conclusion of the study is the motility and progressive motility of spermatozoa in samples with an initial volume of 2 ml is better than samples with an initial volume of 1 ml and 1.5 ml.
Tingkat Keberhasilan Inseminasi Buatan Menggunakan Semen Beku Sexing pada Bangsa Sapi yang Berbeda Wiranto Wiranto; Kuswati Kuswati; Rizki Prafitri; Asri Nurul Huda; Aulia Puspita Anugra Yekti; Trinil Susilawati
Jurnal Agripet Vol 20, No 1 (2020): Volume 20, No. 1, April 2020
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v20i1.15811

Abstract

ABSTRAK. Penelitian ini bertujuan mengevaluasi tingkat keberhasilan IB semen beku sexing Y. Penelitian menggunakan 39 sapi Peranakan Ongole dan 77 sapi Persilangan Limousin yang di IB secara double dosis. IB menggunakan semen beku spermatozoa Y dengan metode pemisahan Sentrifugasi Gradien Densitas Percoll (SGDP) sapi Limousin yang diproduksi oleh Balai Besar Inseminasi Buatan (BBIB) Singosari. Teknik deposisi semen adalah 4+ yaitu pada posisi cornua uteri selanjutnya dilakukan IB pada jam ke 2 dan ke 8. Untuk akseptor dilakukan penyuntikan BioATP+ dan pemberian pakan konsentrat 3 kg per hari selama 3 hari setelah IB. Variabel penelitian meliputi Non Return Rate (NRR), Service Per Conception (S/C), dan Conception Rate (CR). Hasil penelitian menunjukkan bahwa IB sapi Peranakan Ongole dan sapi Persilangan Limousin yaitu NRR1 sebesar 82,05% dan 89,61%, nilai NRR2 sebesar 76,92% dan 84,42%, nilai CR sebesar 58,97% dan 74,03%, serta nilai S/C sebesar 1,78% dan 1,46%. Kesimpulan penelitian adalah IB menggunakan semen beku hasil sexing Y sapi Limousin pada sapi persilangan Limousin memiliki tingkat keberhasilan yang lebih tinggi dibandingkan sapi Peranakan Ongole.(The success rate of artificial insemination using frozen sexing semen of different breed cow)ABSTRACT. This study aimed to evaluate the success rate of AI frozen semen sexing Y. The material in this study used 39 Filial Ongole and 77 Limousin crossbred cattle in AI in double dose. The spermatozoa used were Y frozen semen of Limousin bull with the Percoll Gradient Density Centrifugation (SGDP) separation method produced by the Singosari Center for Artificial Insemination. The semen deposition technique was 4+, which has the position of the cornua uteri (deep Insemination), then AI implemented with a double dose at the 2nd and 8th hours after estrous. The cow acceptor was injected with BioATP+ and feed by 3 kg concentrate per day for three days after AI. Research variables include Non-Return Rate (NRR), Service Per Conception (S/C), and Conception Rate (CR). The results showed that AI of Peranakan Ongol and Limousin crossbred cattle on NRR1, NRR2, CR values, and S/C values respectively was 82.05% and 89.61%; 76.92% and 84.42%; 58.97% and 74.03%; 1.78% and 1.46%. In conclusion, AI using frozen semen from sexing Y Limousin cattle in Limousin crossbred cows had a higher success rate than Filial Ongole cattle.
The success rate of artificial insemination in Limousin crossbred heifer with additional concentrate Putri Utami; Andriawan Soni Hantoro; Rizki Prafitri; Kuswati Kuswati; Asri Nurul Huda; Aulia Puspita Anugra Yekti; Trinil Susilawati
Livestock and Animal Research Vol 22, No 1 (2024): Livestock and Animal Research
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/lar.v22i1.67613

Abstract

Objective: This study aimed to determine whether the addition of concentrate to heifers could be the same as the success of AI in calving cows in terms of Return Rate (NRR), Conception Rate (CR), and Pregnancy Rate (PR).Methods: The material used in this study was 28 Limousine Crossbred cows with 13 cows and 15 heifers. There were two treatments, namely T0: Cows that had given birth with control feed (control without added concentrate) + BIO ATP after AI, while T1: Heifers with control feed and the addition of 1kg/day concentrate for seven days after AI + BIO ATP. Samples were selected by purposive sampling with the criteria of BCS 3-5 (1-9) and aged ≥ 2 years. The artificial insemination method uses rectovaginal with 4+ deposition at the 2nd and 8th hour after estrus, then injection of Bio ATP brand "Rheinbio" intramuscularly as much as 10 ml.Results: The results showed that the reproductive performance of Limousine Crossbred Cow that had given birth (T0) was NRR1 84.62%, NRR2 84.62%, CR 53.33%, and PR 66.67%, while in heifers (T1), it was NRR1 86 .67%, NRR2 60%, CR 20%, and PR 20%.Conclusions: The addition of concentrate feed of 1 kg/head/day for seven days before AI in heifers (T1) showed lower reproductive success than in cows that had given birth (T0) even without the addition of concentrate.
The Successful Artificial Insemination with Frozen Semen from Different Genetic Qualities of Friesian Holstein Bull Yekti, Aulia Puspita Anugra; Hasan, Ismail; Hermanto; Febrianto, Nanang; Isnaini, Nurul; Utami, Putri; Syah, Habib Asshidiq; Wahjuningsih , Sri; Susilawati, Trinil
Jurnal Ilmu-Ilmu Peternakan Vol. 34 No. 3 (2024): December 2024
Publisher : Faculty of Animal Science, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jiip.2024.034.03.15

Abstract

This study aimed to evaluate the success of artificial insemination (AI) using frozen semen with different genetic qualities of Friesian Holstein Bull. In this research, the bulls came from the artificial insemination center of Singosari, consisting of Friesian Holstein bulls with proven bull certificates and the pure breed from embryo transfer. The frozen semen was produced by standard semen processing in the artificial insemination center of Singosari. The inseminated female totals were 56 head of Friesian Holstein crossbred in Pujon district, Malang, Indonesia. The females were divided into two groups: 28 cows receiving insemination from frozen semen of proven bull (T1) and 28 from pure breed FH bull from embryo transfer (T2). The females were chosen with purposive sampling with the criteria, have a Body Condition Score (BCS) of at least 2.5 (scale 1–5), have normal reproductive organs, and exhibit the clear estrus sign. Artificial insemination was performed with a single dose at the eight hour after estrus. Semen deposition was at the corpus uteri, while the thawing process was performed at 22–23°C for 60 seconds. The result showed that the non-return rate 1 (NRR-1), non-return rate 2 (NRR-2), and conception rate (CR) values for AI using frozen semen from a proven bull were 96%, 75% and 43%, respectively. In contrast, the percentage of AI success using pure breed FH bull from embryo transfer was 93%, 86%, and 64%. In conclusion, AI success using pure breed FH bull from embryo transfer was better than proven bull frozen semen.
PELATIHAN PEMASARAN PRODUK MAGGOT MELALUI MARKETPLACE PADA KELOMPOK PETERNAK SAPI PERAH KECAMATAN PUJON KABUPATEN MALANG Isnaini, Nurul; Yekti, Aulia Puspita Anugra; Hamiyanti, Adelina Ari; Sulistyo, Hanief Eko; Andri, Faizal
Kumawula: Jurnal Pengabdian Kepada Masyarakat Vol 8, No 1 (2025): Kumawula: Jurnal Pengabdian Kepada Masyarakat
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/kumawula.v8i1.51103

Abstract

The management of biogas sludge waste from dairy farming operation has not been properly managed, potentially causing environmental pollution. Biogas sludge waste can be utilized as a medium for maggot cultivation, which will yield products such as maggot flour and organic fertilizer, thus realizing a circular economy in dairy farming. This community service program aims to enhance the knowledge and skills of the Dairy Farmer Group in the Pujon District, Malang Regency, in marketing maggot products through online marketplaces. This program was conducted from June to August 2023. The method used in this community service is Participatory Learning and Action, implemented through activities such as socialization, discussions, and hands-on practice. The topics covered during socialization and discussions include the utilization of online marketplaces for marketing maggot products. Participants then engage in hands-on activities, including creating accounts, filling in store profile information, selecting delivery services, filling in bank account details, and uploading products. Participants actively and enthusiastically took part in the program. The initial evaluation results before the program indicate that the knowledge and skills of participants related to the target marketing of maggot products, types of online marketplaces, and marketing management with online marketplaces, scored 80, 65, and 70, respectively. Subsequently, the evaluation after program implementation showed an improvement in knowledge and skills of participants related to target marketing of maggot products, types of online marketplaces, and marketing management with online marketplaces, with scores of 95, 100, and 90, respectively. In conclusion, this community service has successfully enhanced the knowledge and skills of the Dairy Farmer Group in the Pujon District, Malang Regency, in marketing maggot-based products through online marketplaces.Hingga saat ini limbah sludge biogas pada peternakan sapi perah belum dikelola dengan baik sehingga berpotensi menyebabkan pencemaran lingkungan. Limbah sludge biogas dapat dimanfaatkan sebagai media budidaya maggot yang akan menghasilkan produk berupa tepung maggot dan pupuk organik sehingga dapat mewujudkan ekonomi sirkular pada peternakan sapi perah. Tujuan pengabdian kepada masyarakat ini adalah untuk meningkatkan pengetahuan dan keterampilan Kelompok Peternak Sapi Perah Kecamatan Pujon Kabupaten Malang dalam melakukan pemasaran produk budidaya maggot melalui marketplace. Program ini dilaksanakan mulai bulan Juni hingga Agustus 2023. Metode yang digunakan pada pengabdian kepada masyarakat ini adalah Participatory Learning and Action yang dilaksanakan melalui kegiatan sosialisasi, diskusi, dan praktik. Materi yang disampaikan pada saat sosialisasi dan diskusi adalah tentang pemanfaatan marketplace untuk pemasaran produk budidaya maggot. Peserta kemudian melaksanakan praktik mulai dari pembuatan akun, pengisian informasi profil toko, penentuan jasa pengiriman, pengisian rekening bank, dan unggah produk. Peserta terlibat secara aktif dan antusias dalam pelaksanaan program ini. Hasil evaluasi awal menunjukkan bahwa pengetahuan dan keterampilan peserta terkait target pemasaran produk budidaya maggot, jenis marketplace, dan manajemen pemasaran dengan marketplace sebelum dilaksanakan program memiliki skor masing-masing sebesar 80, 65, dan 70. Selanjutnya, evaluasi setelah pelaksanaan program menunjukkan bahwa pengetahuan dan keterampilan peserta terkait target pemasaran produk budidaya maggot, jenis marketplace, dan manajemen pemasaran dengan marketplace mengalami peningkatan dengan skor masing-masing sebesar 95, 100, dan 90. Kesimpulan pengabdian kepada masyarakat ini adalah bahwa kegiatan yang telah dilaksanakan mampu meningkatkan pengetahuan dan keterampilan Kelompok Peternak Sapi Perah Kecamatan Pujon Kabupaten Malang dalam melakukan pemasaran produk budidaya maggot melalui marketplace.
The Quality of Sexed Bali Bull Semen Using Percoll Density Gradient Centrifugation in Various Gradients with Tris Aminomethane Diluent Rahmawati, Nanda Ayu; Yekti, Aulia Puspita Anugra; Wahyudi, Aditiya; Nafis, Fardha Ad Durrun; Isnaini, Nurul; Susilawati, Trinil
Jurnal Ilmu-Ilmu Peternakan Vol. 35 No. 1 (2025): April 2025
Publisher : Faculty of Animal Science, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jiip.2025.035.01.4

Abstract

This research aims to determine the quality of spermatozoa in the sexed semen that separated using the Percoll Density Gradient Centrifugation (PDGC) method with various gradients using egg yolk tris aminomethane diluent. The study was conducted at the Singosari Artificial Insemination Center from December 2023 to February 2024. The study's material was the semen of one Bali bull with individual motility ? 70%. The experimental design used a Randomized Group Design with the sexed semen PDGC method treatment: T1 = 10 gradients, T2 = 5 gradients, and T3 = 3 gradients, each with ten replications. The analysis showed that the gradient variations significantly impact the individual motility on the bottom layer of sexed semen (P<0.05). Conversely, gradient variance did not affect viability, abnormality, concentration, total motile spermatozoa, and individual motility on the top layer of sexed semen (P>0.05). In conclusion, sexed semen using the PDGC method with varied gradients is suitable for the freezing process due to its high quality.
Co-Authors Achadiah Rachmawati Adelina Ari Hamiyanti Ahmad Budi Purnawan Ahmad Salim Ali mahfud Ali Mahfud Alifian Ibnu Ansori Andriani, Nadya Meyta Andriawan Soni Hantoro Ardyah Ramadhina Irsanti Putri Aris Sutomo Aryogi Aryogi Asri Nurul Huda Asri Nurul Huda Aziz, Achmad Fadhli Azmi, Dandy Ulul Bayuardhi, Muhammad Chusnul Hanim Dandy Prasetyo Dedi Muhammad Deny Sulistyowati Dian Ratnawati Dicky Pamungkas Dwi Susan Setiyani Erin Ayu Octaviani Errico Errico Faizal Andri fakhri alfi annashru Febrianto, Nanang Firdaus, Amir Gatot Ciptadi Hanief Eko Sulistyo Hermanto Hisyam Ryandhika Kusuma Ika Fitri Puspitasari Ika Nurjannah Irvan Mardi Ismail Hasan, Ismail Jois Harsah Joko Riyanto Khairul Rasyad Kurniaesa, Titah Ulya Kusmartono Kusmartono Kuswati , Kuswati Kuswati Kuswati Kuswati Lukman Affandhy Sunarto Luqman Affandhy M. Nur Ihsan Mashudi , Mashudi Mashudi Moch Saifudin Muchamad Luthfi Muhammad Ade Salim Muhammad Agus Tahar Muhammad Azharil Faris Muhammad Dikman Muhammad Nur Ihsan Muhammad Zainul Hanif Muhammad Zainul Hanif Nafis, Fardha Ad Durrun Nanang Febrianto Nareswara Hapsari Ndaru Pranowo Widiasmoro Nisa, Dhinda Choirun Nisa’us Sholikah Nolasco da costa Nursahida Ramadhani Nurul Isnaini Nurul Layla Nuryadi Nuryadi Poespitasari Hazanah Ndaru Priyo Sugeng Winarto Putri Utami Putri Utami Putri Utami Putri Utami, Putri Rahmawati, Nanda Ayu Ramadhani, Anisa Ravenska Ravenska Rheta Eva Ramadhani Setiawan Rifai Mustofa Rizki Prafitri Rizki Prafitri Safa, Firlia Safitri, Brilla Ismaya ShikhMaidin, Mashitah Sitiadira Aulia Aldini Sri Wahjuningsih Sri Wahjuningsih Sulistyo, Hanief Eko Sutomo, Aris Syah, Habib Asshidiq Trinil Susilawati Trinil Susilawati Trinil Susilowati Umamah, Azna Roudlotul Nur Wahjuningsih , Sri Wahyudi, Aditiya Widiasmoro, Ndaru Pranowo Willy Saputra Saputra Tatulus Wiranto Wiranto Yadi Malda