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All Journal Buletin Peternakan Jurnal Ilmu Ternak Jurnal Ilmu-Ilmu Peternakan (Indonesian Journal of Animal Science) Jurnal Ternak Tropika JITRO (Jurnal Ilmiah dan Teknologi Peternakan Tropis) Kumawula: Jurnal Pengabdian Kepada Masyarakat REKASATWA : Jurnal Ilmiah Peternakan Livestock and Animal Research Jurnal Agripet

Aulia Puspita Anugra Yekti

Dosen Produksi Ternak Fakultas Peternakan, Universitas Brawijay

Author-ID : 2845970

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Social Sciences Veterinary Other

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Kapasitasi Spermatozoa Sapi Peranakan Ongole dalam Berbagai Formulasi Pengencer Air Kelapa Selama Simpan Dingin Dedi Muhammad; Nurul Isnaini; Kuswati Kuswati; Aulia Puspita Anugra Yekti; Muchamad Luthfi; Lukman Affandhy Sunarto; Trinil Susilawati
Jurnal Ilmu dan Teknologi Peternakan Tropis Vol 6, No 1 (2019): JITRO, Januari
Publisher : Universitas Halu Oleo

ABSTRAKPengencer dasar air kelapa merupakan solusi dari sulit dan mahalnya harga pengadaan bahan baku pembuatan pengencer. Tujuan dari penelitian ini adalah untuk mengetahui kapasitasi spermatozoa sapi Peranakan Ongole dalam berbagai formulasi pengencer air kelapa selama simpan dingin pada suhu 4-5oC. Metodologi yang digunakan adalah eksperimantal laboratorium menggunakan rancangan acak kelompok (RAK). Terdapat tiga perlakuan yaitu, P1 = Pengencer air kelapa + 20% kuning telur, P2 = Pengencer air kelapa + 20% kuning telur + 0,4% putih telur + fruktosa 1000 mg/l. P3 = Pengencer air kelapa + 20% kuning telur + 0,4% putih telur + fruktosa 2000 mg/l, dengan 10 ulangan. Hasil menunjukkan bahwa tidak terdapat perbedaan yang signifikan pada persentase spermatozoa belum kapasitasi, terkapasitasi dan telah rekasi akrosom antara perlakuan formulasi pengencer air kelapa yang berbeda (P1, P2, P3) selama peyimpana suhu 2-5oC. Pengencer berbasis air kelapa mampu mempertahankan kualitas akrosom diatas 50% penyimpanan hari ke lima.Kata kunci : air kelapa, kapasitasi, reaksi akrosom, semen cairABSTRACTCoconut water extender is the solution to the difficulty and high cots of semen diluents. The objective of this study were to examine the capacitation of Ongole Crossbreed sperm in various formulations of coconut water extender during storage in 4-5 oC. The study method used laboratory experimental. The experimental design was Randomized Completely Block Design and the data were analyzed by Analyze of Variance. There are three treatments in this study (P1 = Coconut Water + 20% of yolk, P2 = Coconut Water + 20% of yolk + 0,4% white egg + fructose 1 mg/ml, dan P3 = Coconut Water + 20% of yolk + 0,4% white egg + fructose 2 mg/ml) and ten replications of each treatment. There were not significant differences in percentage of sperm uncapacitation, sperm capacitaion and sperm acrosom reaction between various formulations of coconut water diluents during chilled storage at 4-5 oC. Coconut water diluents are able to maintain quality of sperm acrosom up to day 5th with values above 50%.Keywords: acrosom reaction, capacitation, coconut water, liquid semen

Kualitas Semen Beku Sexing Sapi Peranakan Ongole Menggunakan Volume Semen Awal Yang Berbeda Irvan Mardi; Aulia Puspita Anugra Yekti; Kuswati Kuswati; Muchamad Luthfi; Trinil Susilawati
Jurnal Ilmu dan Teknologi Peternakan Tropis Vol 7, No 3 (2020): JITRO, September
Publisher : Universitas Halu Oleo

ABSTRAKInseminasi buatan dengan menggunakan semen sexing diharapkan menghasilkan pedet dengan jenis kelamin sesuai harapan. Tujuan dari penelitian ini adalah untuk mengetahui kualitas, proporsi, dan jumlah produksi straw sexing menggunakan metode sentrifugasi gradien densitas percoll dengan volume awal semen yang berbeda. Penelitian dilakukan di Loka Penelitian Sapi Potong, Kecamatan Grati, Kabupaten Pasuruan dan Laboratorium Reproduksi Ternak, Fakultas Peternakan Universitas Brawijaya, Malang. Materi yang digunakan adalah semen sapi peranakan ongole berumur berkisar lima tahun dan bobot badan berkisar 700 kg sebanyak tiga ekor, motilitas massa ≥2+ dan motilitas individu ≥70%. Metode yang digunakan adalah eksperimental dengan tiga perlakuan volume awal saat sexing, yaitu 1 (P1); 1,5 (P2); dan 2 (P3) ml dengan ulangan 11 kali (ulangan berfungsi sebagai kelompok). Data dianalisa menggunakan Rancangan Acak Kelompok (RAK). Hasil penelitian menunjukkan bahwa perbedaan volume awal semen tidak berpengaruh (menurun) terhadap motilitas, viabilitas, abnormalitas, konsentrasi, total spermatozoa motil, recovery rate dan proporsi spermatozoa (P>0,05). Pengaruh yang sangat nyata (meningkat) terhadap jumlah produksi straw (P<0,01). Ulangan penelitian ini memberikan pengaruh yang sangat nyata (meningkat) terhadap kualitas (motilitas, konsentrasi, viabilitas, abnormalitas, total spermatozoa motil, RR, proporsi dan jumlah straw) dan proporsi spermatozoa X dan Y (P<0,01). Total spermatozoa motil setiap perlakuan telah memenuhi nilai harapan (10 juta/straw). Proporsi spermatozoa X dan Y telah memenuhi nilai harapan (80%:20%).Kata Kunci: kualitas, proporsi, semen beku sexing, strawABSTRACTArtificial insemination using sexing semen is expected to produce calves with the expected sex. The aim of this study was to determine the quality, proportion, and quantity of sexing semen production using the percoll density gradient centrifugation method with different initial semen volumes. The research was conducted at the Beef Cattle Research, Grati District, Pasuruan Regency, East Java Province, Indonesia, and the Animal Reproduction Laboratory, Faculty of Animal Science, University of Brawijaya, Malang, East Java Province, Indonesia. The material used was semen from three Ongole crossbred bull aged around five years and the bodyweight of around 700 kg, mass motility of ≥2+, and individual motility ≥70%. The method used was experimental with three initial volume treatments during sexing, namely 1 (P1); 1.5 (P2), and 2 (P3) ml with 11 replications (replications function as groups). The data were analyzed using a randomized block design (RBD). The results showed that the treatment of differences in initial semen volume did not affect motility, viability, abnormalities, concentration, total motile sperm, recovery rate, and proportion of sperm (P>0.05). On the other hand, the difference in the initial volume of semen had a very significant effect (increased) on the amount of frozen semen production (P<0.01). Repeated research also had a very significant effect (increased) on the semen quality (motility, concentration, viability, abnormality, total sperm motility, recovery rate proportion, and straw production) and the proportion of spermatozoa X and Y (P<0.01). The total motile sperm for each treatment had met the expected value (10 million/straw). Proportions of spermatozoa X and Y have met the expected value (80%: 20%).Keywords: proportion, quality, sexing frozen semen, straw.

Evaluasi Tingkat Keberhasilan Inseminasi Buatan Double Dosis (Jam Ke 2 Dan Ke 8) Terhadap Kualitas Berahi Pada Sapi Persilangan Ongole Hisyam Ryandhika Kusuma; Kuswati ,; Asri Nurul Huda; Rizki Prafitri; Aulia Puspita Anugra Yekti; Trinil Susilawati
Jurnal Ilmu Ternak Vol 21, No 2 (2021): December
Publisher : Fakultas Peternakan, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/jit.v21i2.33594

AbstrakPenelitian ini bertujuan untuk mengetahui evaluasi tingkat keberhasilan IB double dosis terhadap kualitas berahi pada selang waktu IB jam ke 2 dan jam ke 8 pada sapi Peranakan Ongole. Penelitian dilaksanakan di Desa Senggreng, Kabupaten Malang pada Juli-Oktober 2020. Materi yang digunakan sapi betina persilangan Ongole Crossbred sebanyak 25 ekor yang dipilih secara purposive. Semen yang digunakan adalah semen beku dari Limousine jantan produksi Balai Besar Inseminasi Buatan (BBIB) Singosari, Malang. Metode yang digunakan dalam penelitian ini adalah studi kasus, yaitu dengan memilih sapi sesuai kriteria dan memiliki kualitas berahi yang baik. Hasil penelitian menunjukkan persentase lendir servik (ada, basah, banyak) NRR1 84,62%, NRR2 76,92%, CR 53,85% dan PR 76,92%. Persentase warna vulva merah yaitu NRR1 90%, NRR2 80%, CR 55,56% dan PR 80%. Persentase suhu >38oC NRR1 93,33%, NRR2 86,67%, CR 53,33% dan PR 66,67%. Indikator pH 8 menunjukkan persentase NRR1 92,86%, NRR2 92,86%, CR 57,14% dan PR 64,29%.

Tingkat keberhasilan Inseminasi Buatan (IB) menggunakan semen beku hasil sexing pada Sapi Persilangan Ongole Ali mahfud; Trinil Susilawati; Aulia Puspita Anugra Yekti; Kuswati Kuswati
Jurnal Ilmu-Ilmu Peternakan (Indonesian Journal of Animal Science) Vol 29, No 2 (2019): Agustus
Publisher : Faculty of Animal Science, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jiip.2019.029.02.10

The purpose of artificial insemination using sexing semen was to obtain the calf according to the expected sex. This study aimed to determine the success of artificial insemination in non-sexing and sexing of frozen semen. The materials used in this research were 77 non-sexing artificial insemination acceptor cows and 77 sexing artificial insemination acceptor cows with double dose treatment. The method used in this study was experimental field experiment with the research treatment: P0 = frozen non-sexing semen straw and P1 = frozen semen of spermatozoa Y by sexing method using Percoll Density Gradient Centrifugation (SGDP) produced by Balai Besar Inseminasi Buatan (BBIB) Singosari. The variables of this study were Non Return Rate (NRR), Service Per Conception (S/C) and Conception Rate (CR). The data obtained were analyzed descriptively. The results of the study in each non-sexing and sexing artificial insemination treatment were: the percentage of NRR 1 were 90.91% and 77.92%, followed by NRR 2 with 88.31% and 76.62%, the pregnancy percentage were 84.42% and 75.33%, and the CR values were 79.22% and 57.14%. The conclusion of this study was artificial insemination using frozen non-sexing semen had a higher success rate compared to artificial insemination using frozen sexing semen.

Pengaruh perbedaan waktu inseminasi buatan terhadap keberhasilan kebuntingan sapi Brahman Cross fakhri alfi annashru; M. Nur Ihsan; Aulia Puspita Anugrah Yekti; Trinil Susilawati
Jurnal Ilmu-Ilmu Peternakan (Indonesian Journal of Animal Science) Vol 27, No 3 (2017): Desember
Publisher : Faculty of Animal Science, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jiip.2017.027.03.03

The purpose of this research is to acknowledge the difference of AI time towards Brahman Cross Cow’s pregnancy rate determined by time and Non Return Rate (NRR). The subjects in this research are 75 female Brahman Cross. Methods that were used during this research are fiels testing. Selection method of the sample livestock used is Purpose Sampling with criterias : Female adult cow with weight >250kg, aged 1,5 – 2 years, healthy, and free from any disease, and at least have mating urge. Materials used during this research are frozen cow semen that acquired from Balai Inseminasi Buatan (BIB) Lembang, Bandung, West Java widh 35-40% motility. The variabels are Cow’s pregnancy and mating urge’s symptomps. Data acquired duringthis reserch are analyzed descriptively and chi-square tested. Supportive datas are food and valuefrom heat detector (HD). Research result shows that Brahman Cross species which AI within 0-4 hours interval have higher CR according to NRR1, which is 70%. Compared with the other breed that AI within 8-12 hour interval, which had 37,14% CR value. According to chi-square test, the difference of AI time of Brahman Cross are p<0.05 which means that the difference of AI time of cow is affecting the pregnancy rate. It is advised that AI is executed during 0-4 hour interval after showing mating symptomps and further reviewed about the difference between AI execution time of Brahman Cross and further observation of NRR2 until NRR3 and also Pregnancy Test to know the pregnancy success rate of Brahman Cross.

Pengaruh penggantian Bovine Serum Albumin (BSA) dengan putih telur pada pengencer CEP-2 terhadap kualitas semen sapi Peranakan Ongole pada suhu penyimpanan 3-5oC Nisa’us Sholikah; Nurul Isnaini; Aulia Puspita Anugra Yekti; Trinil Susilawati
Jurnal Ilmu-Ilmu Peternakan (Indonesian Journal of Animal Science) Vol 26, No 1 (2016)
Publisher : Faculty of Animal Science, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jiip.2016.026.01.2

Purpose of this research was to investigate the effect of Bovine Serum Albumin substitution by albumen on CEP-2 to semen quality Ongole CrossBred bull stored at 3-5oC. Research was conducted at Research Centre Beef Cattle Laboratory, Grati, Pasuruan on February 2016. Semen diluent was divided into two groups, there were P0 (90% CEP-2 + 10% Egg Yolk (EY)); and P1 (90% CEP + 0.4% albumen + 10% EY). Data of the research were analyzed using paired design t test. The result showed that after eight days chilled preservation, percentage of motility P1 (47.4±10.9%) was higher than P0 (47±9.2%). Percentage of viability P0 (83.1±1.9%) was higher than P1 (81.3±1.5%). Percentage of abnormality P1 (3.6±0.4%) was lower than P0 (3.8±0.3%). Total motile sperm count after six days chilled preservation was significantly higher in all treatments compared to the standard criteria of SNI 40% motile sperm/ml. The conclusion of this research was 0,4% albumen could replace the BSA capability on CEP-2. Keywords: CEP-2, Bovine Serum Albumin, chilled preservation, semen quality

Evaluation of adoption rate of frozen sexed semen artificial insemination technology adoption in Palang Village, Tuban Regency, East Java Rizki Prafitri; Priyo Sugeng Winarto; Aulia Puspita Anugra Yekti; Trinil Susilawati; Kuswati Kuswati
Jurnal Ilmu-Ilmu Peternakan (Indonesian Journal of Animal Science) Vol 31, No 1 (2021): April 2021
Publisher : Faculty of Animal Science, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jiip.2021.031.01.10

A research group of Animal Science Faculty of Brawijaya University introduced and implemented Frozen sexed semen Artificial Insemination to 120 beef cattle farmers in Palang Village, Tuban Regency, East Java, from 2017 to 2019. The result of the program reported that the success rate of the technology is quite high, with the birth rate of male claves up to 80%. However, farmers’ adoption rate of the technology was relatively low. This research aims to evaluate factors that affected the adoption rate including the technology, characteristics of respondents, and the extension agents. Primary data were collected through a census of 120 farmers involved in the program and in-depth interviews with stakeholders. Secondary data were collected through related documents including reports of the programs, Statistics, and other related documents. This research utilized Quantitative and qualitative analyses. Data of the study indicated that respondents have neutral perceptions of the Frozen Semen Sexing Artificial Insemination. Although male calves relatively have a higher price, more than 50% of respondents did not expect specific sexing for the calves. In-depth interview data revealed that the success of the Artificial insemination technology is more valuable for the farmers rather than the sexing. Characteristics of respondents and the extension agents played significant roles in the adoption rate of the Frozen Sexed Semen Artificial Insemination technology adoption in Palang Village, Tuban Regency, East Java.

Pengaruh kastrasi terhadap performan produksi Sapi Persilangan Wagyu berdasarkan umur yang berbeda Kuswati Kuswati; Ravenska Ravenska; Nareswara Hapsari; Aulia Puspita Anugra Yekti; Trinil Susilawati
Jurnal Ilmu-Ilmu Peternakan (Indonesian Journal of Animal Science) Vol 26, No 3 (2016)
Publisher : Faculty of Animal Science, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jiip.2016.026.03.08

The purpose of this research was to know the effect of castration to Wagyu crossbreed cattle production performance based on age difference and know the appropriate age to do castration. The materials used in this research were 37 heads of 3-month old and 28 heads 4 months calf castration. The method was case studies with purposive sampling. Data were analyzed using unpaired t test. The results showed that the calf castration age of 3 months and 4 months showed significantly different (P <0.05) in daily body weight gain and feed conversion. The average daily weight gain of 3 months old calf castration was 0.78 kg / head / day and 4 months old calf castration was 0.85 kg / head / day and feed conversion of 3 months old calf castration was 5.14 and 4 months old calf castration was 4.48, while the feed intake of the research results were not significantly different. The average feed consumption of 3 months old calf castration was 3.82 kg / head / day and 4 months old calf castration was 3.66 kg / head / day. Calves were castrated on 4 months old showed higher daily weight gain than the calf castration on 3 months old. This could be concluded that 4 months old was more appropriate to do castration. Keywords: Average daily gain, calf stock, feed convertion, castration

Keberhasilan inseminasi buatan menggunakan semen sexing beku pada Sapi Persilangan Ongole Dwi Susan Setiyani; Aulia Puspita Anugra Yekti; Kuswati Kuswati; Trinil Susilawati
Jurnal Ilmu-Ilmu Peternakan (Indonesian Journal of Animal Science) Vol 28, No 3 (2018): Desember
Publisher : Faculty of Animal Science, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jiip.2018.028.03.09

The Artificial Insemination (AI) results in Beef cattle are expected to be male calf because they can increaseweight gain rapidly, so in this study AI was carried out by using sexing semen with Percoll density gradient centrifugation method containing Y sperm.This study was conducted to evaluate the success rate of AI using frozen sexing semen on Ongole Cross Breed. The study method used wasexperimental, comparing AI frozen sexing semen and frozen semen in 54 Ongole Cross Breed. The study variableswere Non Return Rate1 (NRR1), Non Return Rate2 (NRR2) and Conception Rate (CR).The study showed that the success rate of based on the value of NRR1 and NRR2AI using frozen sexing semen on Ongole Cross Breed is lower than un sexed frozen semen with the NRR1 value is 73,91%; 93,54% while the value of NRR2 was 69,56%; 83,87%. Based on CR value of frozen sexing semen higher than un sexed frozen semen showed CR valuewas 43,47%; 35,48%.

Keberhasilan inseminasi buatan menggunakan semen beku dan semen cair pada sapi Peranakan Ongole Trinil Susilawati; Nurul Isnaini; Aulia Puspita Anugra Yekti; Ika Nurjannah; Errico Errico; Nolasco da costa
Jurnal Ilmu-Ilmu Peternakan (Indonesian Journal of Animal Science) Vol 26, No 3 (2016)
Publisher : Faculty of Animal Science, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jiip.2016.026.03.03

The purpose of this research was to know about the successfully artificial insemination (AI) using frozen and liquid semen of Ongole cross (PO) cattle indicated by Service per Conception (S/C), Days Open (DO) dan Conception Rate (CR). The materials used were 60 heads of Ongole crossbred cattle which were selected purposively using some criteria, such as having calving experience, healthy, and free from any reproduction disorder. This field experiment consisted of direct observation and interview to acquire primary and secondary data. Datas were analyzed descriptively and continued by a paired t-test. The results showed that the value of (S/C) was very significantly different (P<0.01), DO was not significantly different (P>0.05). CR for P1, P2, P3, and P4 was found 63.33% , 86.67%, 16.33%, 83.33% respectively. The conclusion of this research was AI using frozen and liquid semen with only 5 days preservation had a difference on the value of service per conception. The most successfull AI was found higher in the liquid semen which was stored for 1 and 5 days than that of frozen semen. Key words : semen, service per conception, days open, calving interval

Co-Authors Achadiah Rachmawati Adelina Ari Hamiyanti Adelina Ari Hamiyanti Ahmad Budi Purnawan Ahmad Salim Ali mahfud Ali Mahfud Alifian Ibnu Ansori Amir Firdaus Andriawan Soni Hantoro Anisa Ramadhani Ardyah Ramadhina Irsanti Putri Aris Sutomo Aryogi Aryogi Asri Nurul Huda Asri Nurul Huda Aziz, Achmad Fadhli Azna Roudlotul Nur Umamah Budi Hartono Chusnul Hanim Dandy Prasetyo Dandy Ulul Azmi Dedi Muhammad Deny Sulistyowati Dewi Masyithoh Dian Ratnawati Dicky Pamungkas Dwi Susan Setiyani Erin Ayu Octaviani Errico Errico Faizal Andri fakhri alfi annashru Febrianto, Nanang Firlia Safa Gatot Ciptadi Habib Asshidiq Syah Hanief Eko Sulistyo Hermanto Hisyam Ryandhika Kusuma Ika Fitri Puspitasari Ika Nurjannah Irvan Mardi Ismail Hasan, Ismail Jois Harsah Joko Riyanto Khairul Rasyad Kurniaesa, Titah Ulya Kusmartono Kusmartono Kuswati , Kuswati Kuswati Kuswati Kuswati Lukman Affandhy Sunarto Luqman Affandhy M. Nur Ihsan Mad Nasir Shamsudin Mashitah ShikhMaidin Mashudi , Mashudi Mashudi Moch Saifudin Muchamad Luthfi Muhammad Ade Salim Muhammad Agus Tahar Muhammad Azharil Faris Muhammad Bayuardhi Muhammad Dikman Muhammad Helmi Muhammad Nur Ihsan Muhammad Zainul Hanif Muhammad Zainul Hanif Nadya Meyta Andriani Nafis, Fardha Ad Durrun Nanang Febrianto Nanang Febrianto Nanang Febrianto Nareswara Hapsari Ndaru Pranowo Widiasmoro Nisa, Dhinda Choirun Nisa’us Sholikah Nolasco da costa Nursahida Ramadhani Nurul Isnaini Nurul Isnaini Nurul Layla Nuryadi Nuryadi Poespitasari Hazanah Ndaru Priyo Sugeng Winarto Puji Akhiroh Putri Utami Putri Utami Putri Utami Putri Utami Putri Utami, Putri Rahmawati, Nanda Ayu Ravenska Ravenska Rheta Eva Ramadhani Setiawan Rifai Mustofa Rizki Prafitri Rizki Prafitri Safitri, Brilla Ismaya Sitiadira Aulia Aldini Sri Wahjuningsih Sri Wahjuningsih Sulistyo, Hanief Eko Syah, Habib Asshidiq Trinil Susilawati Trinil Susilawati Trinil Susilawati Trinil Susilawati Trinil Susilawati Trinil Susilowati Wahjuningsih , Sri Wahyudi, Aditiya Willy Saputra Saputra Tatulus Wiranto Wiranto Yadi Malda

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