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The effect of soybean extract supplementation to tris aminomethane–egg yolk extender on the post-thawing quality of Simmental semen Sri Wahjuningsih; Muhammad Nur Ihsan; Aulia Puspita Anugra Yekti; Muhammad Agus Tahar
Jurnal Ilmu-Ilmu Peternakan (Indonesian Journal of Animal Science) Vol 30, No 3 (2020): December 2020
Publisher : Faculty of Animal Science, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jiip.2020.030.03.10

Abstract

This research aims to evaluate the effect of soybean extract (Glycine max (L.) Merr.) supplementation on tris aminomethane egg yolk extender to the post thawing quality of Simmental bull frozen semen. The fresh semen was collected from 3 Simmental bulls aged at 3 to 3.5 years for twice a week by using artificial vagina. The semen was then selected for sperm motility and abnormality, and the semen that had >70% motility and <15% abnormality was used for the research. The research was conducted in a completely randomized with 4 treatments and 30 replications. The research treatments include 0% (T0), 7.5% (T1), 10% (T2), and 12.5% (T3) soybean extract supplementation on tris aminomethane egg yolk extender. The observed variables include spermatozoa motility, viability, abnormality, and plasm membrane integrity. The collected data were analysed with ANOVA and followed with Duncan’s Multiple Range Test to determine significant differences. The results showed that soybean extract supplementation gave significant effect (P<0.05) to the post thawed quality of Simmental semen. The research concludes that 10% (v/v) soybean  supplementation on tris aminomethane egg-yolk extender was effective to maintain sperm motility, viability, and plasma membrane integrity of post thawed Simmental semen.
Perbedaan Keberhasilan Inseminasi Buatan Menggunakan Semen Beku Dan Semen Cair Menggunakanpengencer Cep-3 + Kuning Telurpada Sapi Persilangan Ongole Ahmad Salim; Aulia Puspita Anugra Yekti; Kuswati Kuswati; Trinil Susilawati
TERNAK TROPIKA Journal of Tropical Animal Production Vol 19, No 1 (2018): TERNAK TROPIKA Journal of Tropical Animal Production
Publisher : Jurusan Produksi Ternak, Fakultas Peternakan, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (336.333 KB) | DOI: 10.21776/ub.jtapro.2018.019.01.10

Abstract

Abstract: The purpose of this research was to investigate the successful difference of artificial insemination (AI) using frozen semen and liquid semen with CEP-3 + egg yolk diluent of Ongole Crossbred cow indicated by Non Return Rate (NRR) and Conception Rate (CR). The materials used were 54 heads of cow which selected purposively using some criteria such as healthy, free from any reproduction disorder and having at least sexual desire criteria 3A (red, swollen and warm). This field experiment consisted of direct observation and interview to acquire primary and secondary data. The datas were analyzed descriptively and continued by   Chi-square test. The results showed that the value of NRR was insignificant influenced between the two treatment. The Conception Rate for frozen semen and liquid semen was found 62.96% and 51.85% respectively. The conclusion of this experiment was the successful rate of artificial insemination (AI) using liquid semen with the CEP-3 + 20% egg yolk diluent was lower than artificial insemination using frozen semen. The value of Conception Rate of frozen semen treatment was 62.96% and the liquid semen treatment was 51.85%.
TINGKAT KEBERHASILAN INSEMINASI BUATAN DOUBLE DOSIS PADA SAPI PERSILANGAN ONGOLE DENGAN KUALITAS BERAHI YANG BERBEDA Alifian Ibnu Ansori; Kuswati kuswati; Asri Nurul Huda; Rizki Prafitri; Aulia Puspita Anugra Yekti; Trinil Susilawati
REKASATWA : Jurnal Ilmiah Peternakan Vol 3, No 1 (2021): REKASATWA : Jurnal Ilmiah Peternakan
Publisher : Universitas Islam Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33474/rekasatwa.v3i1.11820

Abstract

Tujuan dari penelitian ini untuk mengetahui tingkat keberhasilan inseminasi buatan double dosis pada jam ke-8 dan jam ke-16 pada sapi Persilangan Ongole dengan kualitas berahi yang berbeda. Materi dalam penelitian ini menggunakan 25 ekor sapi betina Persilangan Ongole yang dipilih secara purposive. Metode dalam penelitian ini adalah observasi langsung di lapang, dengan menyeleksi sapi betina dengan kriteria BCS >3, umur >1,5 tahun dan telah melahirkan. Penelitian ini menggunakan semen beku pejantan Limousin yang diproduksi oleh Balai Besar Inseminasi Buatan (BBIB) Singosari, Malang. Hasil penelitian menujukkan persentase karakteristik yaitu Non Return Rate (NRR-1, NRR-2) masing-masing 92,31% dan 58,33%, Conception Rate (CR) 38,46% dan 25%, Pregnancy Rate (PR) 53,85% dan 50%. Suhu Vagina 37,0-37,9ºC dan >38,0ºC NRR-1, NRR-2 masing-masing 77,78% dan 57,14%, CR 14,28% dan 33,33%, PR 42,86% dan 55,56%. Lendir servik (ada, basah, sedikit) dan (ada, basah, banyak) NRR-1, NRR-2 masing-masing 89,47% dan 16,67%, CR 31,58% dan 16,67%, PR 47,37% dan 66,67%. Lendir Servik pH 7 dan pH 8 NRR-1, NRR-2 masing-masing 77,78% dan 68,75%, CR 11,11% dan 37,50%, PR 44,44% dan 56,25%. Nilai HD 21-30 dan 31-40 NRR-1, NRR-2 masing-masing 50% dan 82,35%, CR 12,50% dan 35,29%, PR 50% dan 52,94%. Kesimpulan penelitian ini bahwa karakteristik warna vulva merah, suhu vagina >38ºC, karakteristik lendir (ada, basah, banyak), lendir servik pH 8 dan nilai HD 31-40, memberikan persentase kebuntingan yang lebih tinggi.
Effect of Body Condition Score Limousine Crossbred Cow on Cattle Oestrus Response Synchronized using PGF2α Single Dose Muhammad Zainul Hanif; Putri Utami; Aulia Puspita Anugra Yekti; Asri Nurul Huda; Joko Riyanto; Chusnul Hanim; Mashudi Mashudi; Trinil Susilawati
Buletin Peternakan Vol 46, No 2 (2022): BULETIN PETERNAKAN VOL. 46 (2) MAY 2022
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v46i2.72976

Abstract

One  of  the  strategies  to  increase  the  cattle  population  in  Indonesia  is  the  AI (Artificial   Insemination)  method.  The  AI program's  improvement  of   reproductive efficiency can use the oestrus synchronization method. The purpose of this study was to evaluate   the   effect   of   body   weight   and   body   condition   score   on   the   oestrus synchronization  response with  the brand  "lutelyse."  This  research  was  conducted  in  Senggreng  Village,  Sumberpucung  District,  Malang Regency,  This  research  method uses experimental field experiments  with a sampling technique of purposive sampling. This study took a sample of 21, aged 1,5 –5 years, synchronized with PGF2α as much as  5  ml,  which  was  then  carried  out  by  observing  oestrus  behavior  for  24  hours after synchronization.  The  results  showed  that,  the  percentage  of  oestrus in  cows  with body condition  score  (BCS) 4,  which  is  76.92%,  is  lower  than  cows  with  BCS  5,  which  is 100%.  For  the synchronization  distance  with  the  onset  of  oestrus  in  BCS  4  cattle, namely 3802.92 ± 1007.80 (minutes) and BCS 5, namely 4081 ± 884.91 (minutes) with  the results of the conception rate of BCS 4 cattle of 38.46% and BCS 5 of 50%. Based on the results of the study, it was concluded that BCS 5 has a higher success pregnancy rate than BCS 4.
Study of the quality and integrity of spermatozoa acrosome caps in frozen sexing semen Friesian Holstein cattle Sitiadira Aulia Aldini; Nurul Isnaini; Aulia Puspita Anugra Yekti; Trinil Susilawati
Jurnal Ilmu-Ilmu Peternakan (Indonesian Journal of Animal Science) Vol. 32 No. 2 (2022): August 2022
Publisher : Faculty of Animal Science, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jiip.2022.032.02.09

Abstract

Artificial insemination (AI) of dairy cows using sexing semen is the right choice to obtain female calves as replacement stock. The success of AI is influenced by the quality of the spermatozoa used. The purpose of this study was to determine the quality of semen produced by Lembang Artificial Insemination Center. The material used is 30 frozen semen straws of FH cattle produced by Lembang Artificial Insemination Center. This research method uses observational methods. The research variables included individual motility, viability, abnormalities, concentration, total motile spermatozoa, and intact acrosome caps of spermatozoa. The data obtained were analyzed by the Analysis of Variance (ANOVA) test and continued with Duncan's test if significantly different. The results showed that individual motility, concentration, total motile spermatozoa, and intact acrosome caps of spermatozoa had a very significant effect on all treatments (P<0.01), while spermatozoa abnormalities had a significant effect (P<0.05) on all treatments, but on viability, there was no effect (P> 0.05) in all treatments. Motility of individual spermatozoa in non-sexing semen (55.80±4.56)%, sperm X (46.30±5.36)%, sperm Y (46.70±6.40)%. Viability of spermatozoa in non-sexing semen (74.57±4.05)%, sperm X (73.24±2.30)%, sperm Y (72.51±2.68)%. Spermatozoa abnormalities in non-sexing semen (2.73±2.24)%, sperm X (4.94±1.57)%, sperm Y (3.25±2.72)%. Concentration of non-sexing semen (33.88±1.98 million/mini straw), sperm X (25.06±4.29 million/mini straw), sperm Y (25.13±5.74 million/mini straw). The number of sperm motile non-sexing (18.60±1.58 million/mini straw), sperm X (11.68±2.82 million/mini straw), sperm Y (25, 11.64±2.68 million/mini straw). Intact acrosome caps, non-sexing semen (78.40±4.84)%, sperm X (74.08±3.80)%, sperm Y (70.70±4.78)%.
Pengaruh lama thawing dengan air dingin pada semen beku Sapi Peranakan Ongole terhadap kualitas semen Nursahida Ramadhani; Achadiah Rachmawati; Ahmad Budi Purnawan; Trinil Susilawati; Aulia Puspita Anugra Yekti
Livestock and Animal Research Vol 20, No 3 (2022): Livestock and Animal Research
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/lar.v20i3.63170

Abstract

Objective: The purpose of this research was to determine the effect of thawing durations using cold water on Ongole Crossbred Bull frozen semen towards semen quality including motility, viability, abnormality and total motile spermatozoa.Methods: The method used was experimental in the laboratory using five treatments and ten replications on frozen semen samples of Ongole Crossbred Bull. The data were analyzed using Randomized Block Design variance and continued with DMRT if there was a significant difference. Motility and total motile spermatozoa were tested by Chi Square test to compare with the SNI standard for frozen semen quality. In this research, five treatments were carried out, T0 with a thawing time of 30 seconds with a temperature of 37 oC, while T1, T2, T3 and T4 each used a thawing time of 15, 30, 45 and 60 seconds with temperature of 28 oC.Results: The best thawing results were T2 with individual motility 49.9 ± 6.0 %, viability 65.1 ± 7.2 %, abnormality 4.0 ± 1.8 %, concentration of 39.1 ± 10.2 million/straw and total motile spermatozoa 19.3 ± 4.0 million/straw.Conclusions: In conclusion, the duration and temperature of thawing can affected the quality of Ongole Crossbred frozen semen.
Persentase keberhasilan inseminasi buatan dosis ganda pada sapi persilangan Limousin dengan karakter lendir serviks yang berbeda Nurul Layla; Yadi Malda; Aulia Puspita Anugra Yekti; Asri Nurul Huda; Rizki Prafitri; Kuswati Kuswati; Trinil Susilawati
Livestock and Animal Research Vol 20, No 3 (2022): Livestock and Animal Research
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/lar.v20i3.61588

Abstract

Objective: The aim of the study was to find out the successful pregnancy in Limousin crossbreed cattle using double-dose AI method on different characters of cervical mucus.Methods: The material used in this study were 45 female cattle with BCS criteria 3-6 on a scale of 1-9 and aged 1.8-6 years. The parameters observed were the condition of the mucus, the pH of the mucus and the electrical resistance of the mucus. AI method uses a double dose method with 4+ semen deposition (cornua utery).Results: The results showed that the highest percentages of NRR 1 and NRR 2 were shown by cattle with the criteria for mucus conditions being present and abundant. It has a pH value of 8 and an electric current resistance is in the range of 20-29 Ω with a percentage of NRR 1 of 62.22%, 53.33% and 71.11%, while the NRR 2 has a percentage of respectively 60.00%, 51.11% and 68.89%. The highest percentage of CR was also shown by cattle with the mucus character, which had percentages of 33.33%, 33.33% and 42.22%, respectively.Conclusions: It was concluded that the highest percentage of pregnancy was shown by cattle with the criteria of mucus being present and abundant, having a pH value of 8 and having an electric current resistance value in the 20-29 Ω category.
Database of Nutrition Status, Production and Reproduction Performance, and Social Economy of a Farmer of a Beef Cattle Farm in Sumberpucung District, Malang Regency Mashudi; Rizki Prafitri; Nanang Febrianto; Aulia Puspita Anugra Yekti; Asri Nurul Huda; Poespitasari Hazanah Ndaru; Trinil Susilowati
Jurnal Ilmu-Ilmu Peternakan (Indonesian Journal of Animal Science) Vol. 32 No. 3 (2022): December 2022
Publisher : Faculty of Animal Science, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jiip.2022.032.03.06

Abstract

Sumberpucung District, Malang Regency has the potential for developing beef cattle. This study aimed to analyze the nutrition status, production, and reproduction performance of beef cattle as well as examine the socio-economic of farmers. The research method is a survey to obtain a database about beef cattle kept by a farmer. This study involved 50 respondents spread over 4 villages, namely Senggreng, Jatiguwi, Trenyang, and Sambigede. The results of the research showed that the average age of farmers was only 14% who were under 40 years old. The number of cattle ownership was an average of 2-3 heads with the largest breed of cattle being Simmental crossbreeds. The system of breeding and rearing was wholly AI and in the cage. Feed that was usually given in general was rice straw followed by corn straw, sugarcane top, elephant grass,  field grass, and rice bran. The average feed intake of rice straw, corn straw, and elephant grass were 17.865 – 22.9 kg/head/day, 10.23 – 20.5 kg/head/day, and 17-31.3 kg/head/day respectively. The chest girth of the male adult group had the highest value of 162.5 cm and the female adult group of 165.7 cm. The highest estimated live weights for male and female adults in Jatiguwi village were 340.40 kg and 352.31 kg respectively. The highest and the lowest average gestation length were 235 days and 224 days respectively. The highest and the lowest average of S/C were 5.5 times and once. The average calving interval of a cow in Senggreng, Trenyang, Sambigede, and Jatiguwi villages were 440 days, 399 days, 420 days, and 403 days respectively. The number of cattle that were pregnant in the first AI, second AI, and more than the second AI were 27 heads, 32 heads, and 24 heads. Based on the type of feed and its intake, it can be said that the nutrition status of beef cattle in Sumberpucung District is highly good. Effects of nutrition status can be seen from the performance of production and reproduction of cattle in terms of chest girth, live weight, and calving interval.
The Relationship of Estrous Character with Conception Rate in Madura Cattle Kuswati Kuswati; Dandy Prasetyo; Aulia Puspita Anugra Yekti; Trinil Susilawati
Jurnal Ilmu-Ilmu Peternakan (Indonesian Journal of Animal Science) Vol. 32 No. 3 (2022): December 2022
Publisher : Faculty of Animal Science, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jiip.2022.032.03.12

Abstract

This study aimed to determine the relationship between estrous characteristics with the success of AI in Madura cattle. A total of 50 female Madura cattle were used in this research. The estrus quality was observed, including vulva temperature, vulva swelling, vulva color, cervical pH, and cervical mucus. The parameters were NRR-1, NRR-2, Pregnancy Rate, and conception rate. The results showed that Madura cattle with the quality of estrus in the uneven red vulva color category showed better results than red vulva color at NRR-1, NRR-2, CR, and PR values, 100%, 93,33%, 93,33%, and 93,33% respectively. Abundant cervical mucus showed better results than the moderate mucus on the NRR-1, NRR-2, CR, and PR values, which were 100%, 87,5%, 75%, and 75%, respectively. Then a vulva temperature of 37,0-37,9ºC showed better results than a vulva temperature >38ºC in the NRR-1, CR, and PR values, namely 92,12%, 64,71%, and 70,59. Furthermore, the pH condition of cervical mucus with a pH value of 7 showed better results than cervical mucus with a pH of 8 at NRR-1, NRR-2, CR, and PR, 94,12%, 94,12%, 70,59%, and 76,47%. In the very swollen vulva category, the results were better than slightly swollen at the NRR-1 value of 100%. This study concludes that the characteristic uneven color of the vulva, the vulva temperature is 37,0-37,9, the abundant cervical mucus, cervical pH 7, and a very swollen vulva give a high percentage of the success of AI.
Reproductive Profile of Black Soldier Fly (Hermetia Illucens L.) on Different Rearing Substrates as Ruminant Feed Aulia Puspita Anugra Yekti; Nurul Isnaini; Sri Wahjuningsih; Ardyah Ramadhina Irsanti Putri; Hanief Eko Sulistyo
Jurnal Ilmu-Ilmu Peternakan Vol. 33 No. 2 (2023): August 2023
Publisher : Faculty of Animal Science, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jiip.2023.033.02.15

Abstract

There is lack of evidence on the black soldier fly (Hermetia Illucens L.) and its use as a biological control and waste recycle agent. Unknown information related with mating and ovipositional activities. The aim of this research is to determine the reproductive profile and its manure of the Black Soldier Fly (Hermetia Illucens L.) through different rearing substrate as ruminant feed. A total of 4 treatments and 4 replicates were used in this experiment. The treatment diet was formulated as follows:    T0:  dry fermented cow waste, T1: 21% of crude protein, T2: 22% of protein, T3: 23% of protein. The data was analyzed using ODA SAS on an academic general linear model (GLM).  The result showed using different rearing substrates gave insignificant differences (p > 0.05) on the reproductive profile, nutritional content, and manure nutritional content. In summary, the use of different leves of protein can be used as the rearing substrate without any adverse effects.
Co-Authors Achadiah Rachmawati Adelina Ari Hamiyanti Ahmad Budi Purnawan Ahmad Salim Ali Mahfud Ali mahfud Alifian Ibnu Ansori Andriani, Nadya Meyta Andriawan Soni Hantoro Ardyah Ramadhina Irsanti Putri Aris Sutomo Aryogi Aryogi Asri Nurul Huda Asri Nurul Huda Aziz, Achmad Fadhli Azmi, Dandy Ulul Bayuardhi, Muhammad Chusnul Hanim Dandy Prasetyo Dedi Muhammad Deny Sulistyowati Dian Ratnawati Dicky Pamungkas Dwi Susan Setiyani Erin Ayu Octaviani Errico Errico Faizal Andri fakhri alfi annashru Febrianto, Nanang Firdaus, Amir Gatot Ciptadi Hanief Eko Sulistyo Hermanto Hisyam Ryandhika Kusuma Ika Fitri Puspitasari Ika Nurjannah Irvan Mardi Ismail Hasan, Ismail Jois Harsah Joko Riyanto Khairul Rasyad Kurniaesa, Titah Ulya Kusmartono Kusmartono Kuswati , Kuswati Kuswati Kuswati Kuswati Lukman Affandhy Sunarto Luqman Affandhy M. Nur Ihsan Mashudi , Mashudi Mashudi Moch Saifudin Muchamad Luthfi Muhammad Ade Salim Muhammad Agus Tahar Muhammad Azharil Faris Muhammad Dikman Muhammad Nur Ihsan Muhammad Zainul Hanif Muhammad Zainul Hanif Nafis, Fardha Ad Durrun Nanang Febrianto Nareswara Hapsari Ndaru Pranowo Widiasmoro Nisa, Dhinda Choirun Nisa’us Sholikah Nolasco da costa Nursahida Ramadhani Nurul Isnaini Nurul Layla Nuryadi Nuryadi Poespitasari Hazanah Ndaru Priyo Sugeng Winarto Putri Utami Putri Utami Putri Utami Putri Utami, Putri Rahmawati, Nanda Ayu Ramadhani, Anisa Ravenska Ravenska Rheta Eva Ramadhani Setiawan Rifai Mustofa Rizki Prafitri Rizki Prafitri Safa, Firlia Safitri, Brilla Ismaya ShikhMaidin, Mashitah Sitiadira Aulia Aldini Sri Wahjuningsih Sri Wahjuningsih Sulistyo, Hanief Eko Sutomo, Aris Syah, Habib Asshidiq Trinil Susilawati Trinil Susilawati Trinil Susilowati Umamah, Azna Roudlotul Nur Wahjuningsih , Sri Wahyudi, Aditiya Widiasmoro, Ndaru Pranowo Willy Saputra Saputra Tatulus Wiranto Wiranto Yadi Malda