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All Journal Jurnal Sain Veteriner Jurnal Veteriner Media Kedokteran Hewan Jurnal Riset Veteriner Indonesia (Journal of The Indonesian Veterinary Research) Jurnal Medik Veteriner Journal of Parasite Science Ovozoa: Journal of Animal Reproduction Jurnal Kedokteran Hewan
Bambang Sektiari Lukiswanto
Departemen Klinik Veteriner Fakultas Kedokteran Hewan Universitas Airlangga, Surabaya
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Veterinary

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Journal : Jurnal Veteriner

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Aktivitas Antifibrotik Ekstrak Buah Delima Terstandar 40% Ellagic Acid pada Tikus Putih (Rattus norvegicus) sebagai Hewan Model (ANTIFIBROTIC ACTIVITY OF POMEGRANATE FRUIT EXTRACT STANDARDIZED 40% ELLAGIC ACID ON RATS (RATTUS NORVEGICUS) AS ANIMAL MODEL) Wiwik Misaco Yuniarti; Bambang Sektiari Lukiswanto
Jurnal Veteriner Vol 13 No 2 (2012)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The number of patients with chronic liver disease (fibrosis or cirrhosis of the liver) is increasing fromtime to time. However, until now there is no therapy that really effective to overcome that disease. Therapyfor liver fibrosis typically use substance that have antioxidant activity, anti-inflammatory or anti fibrotic.Various efforts always done to find alternative therapies for liver fibrosis. One of them is the use ofpotential plants that suspected having such activity. Various parts of pomegranate have been shown tohave various activities that beneficial for health. This study was conducted to determine the effect ofpomegranate fruit extract standardized 40% ellagic acid on the improvement degree of liver fibrosis causedby cholestasis by measuring of serum alkaline phosphatase levels (ALP) and gamma-glutamyl transferase(GGT), as a specific indicator of liver damage becaused of cholestasis. The research was conducted by using32 male rats, wistar strain, 2.5 month old, weighing between 150-200 grams. Animal models of liver fibrosis obtained by using BDL technique. Subjects were divided into a control group (P0 = without BDLand giving of pomegranate extract) and treatment groups (P1 = BDL with administration of CMC, P2 =BDL with ellagic acid 90% and P3 = BDL with pomegranate fruit extract standardized 40% ellagic acid).CMC, extract (150 mg / kg BW / PO) and ellagic acid (60 mg / kg BW / PO) administered for 21 consecutivedays in the same volume. At the end of 21 days periods, biochemical evaluation was performed to measureserum levels of GGT and ALP. The result indicated that administration of pomegranate fruit extract ( P3)significantly reduced GGT ( 10.5±9.2 mg/dl) and ALP level ( 509.0±4.2 mg/dl) close to normal level of GGTand ALP ( P0, GGT : 2.8 ± 1.4; ALP : 449.0±62.3) (p<0.05). The level of GGT and ALP in P3 group were lowercompared to the group ellagic acid (P2, GGT=48.5±4.8 and ALP = 691.0± 29.7) and group which only begiven CMC (P1, GGT 191.0±35.4 and ALP 890 ± 5.7) ( p<0.05). Extract of pomegranate fruit thatstandardized with 40% ellagic acid is potential as a antifibrotic agent.
HYSTOPATHOLOGIC CHANGES ON AORTA OF CIRRHOSIS MALE RATS (RATTUS NORVEGICUS) INDUCTED BY ESCHERICIA COLI O55 : B5 Tony Hartono; Wiwik Misaco Yuniarti; Bambang Sektiari Lukiswanto
Jurnal Veteriner Vol 9 No 3 (2008)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The background of this study is to visualize histopathological changes on aorta of cirrhosis rats (Rattus norvegicus) induced by endotoxin E. coli O55 : B5 This study was a laboratory experimental using complete randomized design with five treatments and five repetitions. Twenty five male Wistar rats were used as experimental model of cirrhosis by bile duct ligation (BDL) technique. Three weeks after BDL, all cirrhosis experimental models were induced with a single intra venous injection of Eschericia coli endotoxin (3mg/kg b.b in 1 ml sterile saline), except those of five control rats that induced with sterile saline at the same volume only. Aortas of control rats group were excised at 6 hours after induction with sterile saline, whereas the other four groups were done at 6, 12, 18 and 24 hours after induction with endotoxin. The quantity of endothelial cell, discontinuity increment and the thickness of internal elastic lamina layer were observed to know histopathological changes on aorta. Histopathological changes were observed using a light mycroscope, dyscontinuity and thickness of internal elastic lamina were measured by reticular micrometer. The quantity of endothelial cell on control and observation interval of 6 and 12 hours as significant difference (P<0.05), which are bigger than that of 18 and 24 hours. Rats in the control group have the biggest quantity comparing to the other treatments. Discontinuity and thickness of internal elastic lamina layer had significant difference (P<0.05) on control, observation on 6 and 12 hours compared to observation on 18 and 24 hours after being induced with endotoxine. The highest discontinuity and the thinnest elastic lamina internal were obtained within observation on 24 hours. VCAM-1 expression on control group differ from observation on 6 and 12hours but all of them have significant difference to observation on 18 and 24 hours (P<0,05). The decrease of endothelial cell number is caused by endothelial cell contact with endotoxin. Longer contact interval can make more severe injury and dysfunction. When the aorta had loss of its endothelial cell, internal elastic lamina will exposed with endotoxin directly. In the vessel, endotoxin cause nitric oxide (NO) release, that have antiproliferative characteristic for neointimal formation. This condition presumed to be the cause of the dyscontinuity increment and thickness decrement of internal elastic lamina of cirrhosis rats. VCAM-1 expression was influenced by quantity and condition of endothelial cell. The higher VCAM-1 expression in control group higher than the other group because the quntity and morphofunction of endothelial cell was better than that of 6, 12, 18 and 24 hours.
Co-Authors A'la, Rofiqul Aksono HP., Eduardus Bimo Al-Arif, Moh. Anam Anike Rachmawati Anike Rachmawati Arifah, Sitti Aswin, Ahmad Aulia Rakhman, Muhammad Andika Bagaskara, Ryan Boedi Setiawan BUDI UTOMO Diyantoro Diyantoro, Diyantoro E Djoko Poetranto Fedik Abdul Rantam Fitri Nurdiana Helen Susilowati, Helen Hisyam, Mirza Atikah Madarina IMAM MUSTOFA Ira Sari Yudaniayanti Ira Sari Yudaniyanti Kadek Rachmawati Kuncorojakti, Suryo Kusnoto Kusnoto LINA SUSANTI Lucia Tri Suwanti, Lucia Tri M. Zainal Arifin Ma'ruf, Anwar Mufasirin Mustofa Helmi Effendi Niken Hastuti, Apriliwiyani Nisa’ Rachmaningtyas Putri Nove Hidajati Nufus, Faizah Zakiyyatun Nunuk Dyah Retno Lastuti Nusdianto Triakoso Poetranto, Emmanuel Djoko Rahardjo, Adi Prijo Rahmahani, Jola Rahmi Sugihartuti Septian H, Fajar Setiawan Koesdarto Sitti Arifah Soeharsono Sri Mumpuni Sri Mumpuni Sudjono, Budi Santoso Suryaningtyas, Ignasia Friska Amelia Theresia, Sis Wisnuarti Tony Hartono Widiyatno, Thomas Valentinus Widjiati w Wijaya, Andi Yasmin Wiwik Misaco Yuniarti Wiwik Misaco Yuniarti Wiwik Misaco Yuniarti Wiwik Misaco Yuniarti