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All Journal Jurnal Penelitian Saintek Agrikultura Cropsaver : Journal of Plant Protection Jurnal Fakultas Pertanian Dharmakarya : Jurnal Aplikasi Ipteks Untuk Masyarakat Biota Kultivasi Jurnal Mikologi Indonesia AGROLOGIA : Jurnal Ilmu Budidaya Tanaman Indonesian Journal of Community Services Cel Agrikultura Masyarakat Tani: Jurnal Pengabdian kepada masyarakat
Noor Istifadah
Departemen Hama Dan Penyakit Tumbuhan, Fakultas Pertanian, Universitas Padjadjaran
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Engineering Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Medicine & Pharmacology Public Health Other

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The Abilities of Bacillus subtilis and Trichoderma sp. to Suppress Powdery Mildew Disease on Tomato Leaves Istifadah, Noor; Maharani, Febry Aulia Riski
CROPSAVER Vol 6, No 2 (2023)
Publisher : Departemen Hama dan Penyakit Tumbuhan Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/cropsaver.v6i2.49011

Abstract

Powdery mildew caused by Oidium sp. is an important disease on tomatoes cultivated in the greenhouse. Biological control is an environmentally-friendly method for plant disease control. The objective of this study was to examine the abilities of Bacillus subtilis and Trichoderma sp. isolated from compost tea to suppress powdery mildew disease on tomato leaves. The experiments were arranged in randomized complete block design with seven treatments consisting application of B. subtilis, Trichoderma sp., the combination of both microbes, molasses (1%), water and control (untreated leaves). In the first experiment, the treatments were applied on the infected leaves as curative strategy, while in the second experiment the treatments were applied at 7, 4, and 1 day before and every 3 days after pathogen inoculation. The results showed that B. subtilis, Trichoderma sp. or combination of both microbes that were applied in the infected leaves only reduced the disease severity by 29–33%. However, the application of B. subtilis every three days started at 7 days before pathogen inoculation suppressed powdery mildew by 93.7%, whereas combination of B. subtilis and Trichoderma sp. reduced the symptom on inoculated leaves by 85.3%. Spraying tomato leaves with 1% molasses reduced the infection rate and suppressed powdery mildew by 78.6%. Mixing the molasses with Trichoderma sp. suspension (10%, v/v) did not improve the level of suppression. This study showed that application of B. subtilis regularly as preventive measure is very potential for biological control of powdery mildew disease.
Inhibition Effects of Culture Filtrates and Volatile Compounds of Antagonistic Microbes Isolated from Vermicompos and Compost Teas on the Growth of Alternaria solani Sor. in Vitro Istifadah, Noor; Septiandini, Adelia; Hartati, Sri; Widiantini, Fitri
CROPSAVER Vol 5, No 2 (2022)
Publisher : Departemen Hama dan Penyakit Tumbuhan Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/cropsaver.v5i2.43278

Abstract

Alternaria solani Sor. is one of destructive pathogens in solanaceous plants including tomato. Bacteria and yeast isolated from water extract of organic matters are potential as biological control agents of plant pathogenic fungi. Mechanisms of antagonism of bacteria and yeast can be through antibiosis. This study was conducted to examine the abilities of culture filtrate and volatile compounds produced by antagonistic bacteria and yeast isolated from compost and vermicompost teas to inhibit the growth of A. solani in vitro. The experiments were arranged in randomized complete design with four replications. The culture filtrate experiment applied well diffusion method, while the volatile compound effect experiment used petri dish sandwich method. The results showed that the culture filtrates of four bacteria and three yeast isolates inhibited the growth of A. solani in vitro by 16.6-87.5%. The highest inhibition level was showed by KSB4 isolate (Bacillus subtilis), a bacterial isolate from cow manure compost tea. In the volatile compound effect experiment, the tested bacteria and yeast isolates inhibited the pathogen growth by 31.3-75.2%, with the highest inhibition was showed by KcB3, a bacterial isolate from vermicompost tea. The isolate that its culture filtrate and volatile compounds both showed high inhibition level (62.7% and 87.5%) on A. solani growth was KSB4 isolate (B. subtilis).
Capability of Three Yeast Species in Suppressing Green Mold (Penicillium digitatum) on Siam Citrus Fruit (Citrus nobilis) Hartati, Sri; Utari, Elinda Dwi; Rasiska, Siska; Istifadah, Noor
CROPSAVER Vol 5, No 2 (2022)
Publisher : Departemen Hama dan Penyakit Tumbuhan Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/cropsaver.v5i2.42173

Abstract

Green mold is one of the main diseases in citrus fruits caused by Penicillium digitatum. One of the managements that can be used to control this disease is by using yeasts as antagonistic agents. The objective of this study was to evaluate the ability of yeast to suppress Green mold caused by P. digitatum in siam citrus (Citrus nobilis Lour.). The study was conducted using a completely randomized design. The treatments were the application of yeast, consisting of Aureobasidium pullulans isolate Dmg 11 DEP, Rhodotorula minuta isolate Dmg 16 BE, Candida tropicalis isolate Lm 13 BE, fungicide benomyl, and control. The results showed that A. pullulans isolate Dmg 11 DEP, C. tropicalis isolate Lm 13 BE, and R. minuta isolate Dmg 16 BE were able to suppress the growth of P. digitatum in vitro on dual culture method, with suppression levels ranged from 14.64%-21.02%. The greatest suppression was caused by R. minuta isolate Dmg 16 BE. The formation of volatile compounds test results were ranged from 14.51%-34%, and the highest suppression was on C. tropicalis isolate Lm 13 BE. Application of the yeasts on siam citrus fruit was able to reduce the pathogen’s growth, and the suppressions were 28.87% to 68.72%. R. minuta isolate Dmg 16 BE caused the highest suppression.
Toksisitas Minyak Biji Azadirachta indica A. Juss Dengan Bahan Sinergis Sesamum indicum L. Terhadap Spodoptera frugiperda J.E. Smith Maulana, Raihan Rahmat; Dono, Danar; Istifadah, Noor
Agrimasta Vol 3, No 1 (2025): November
Publisher : Fakultas Pertanian, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/agrimasta.v3i1.68282

Abstract

Spodoptera frugiperda adalah hama pada tanaman jagung yang dapat mengakibatkan kerugian yang besar bagi para petani jagung. Minyak biji nimba (Azadirachta indica A. Juss) dapat digunakan sebagai insektisida nabati karena memiliki salah satu senyawa yang bersifat toksik. Minyak wiijen (Sesamum indicum L.) diketahui dapat meningkatkan keefektifan suatu insektisida karena salah satu senyawa yang bersifat sinergis yaitu sesamin. Penelitian ini bertujuan untuk mengevaluasi pengaruh toksik campuran minyak biji nimba dan minyak wijen terhadap larva S. frugiperda dan mengkaji sifat sinergis minyak wijen terhadap minyak biji nimba. Penelitian ini terdiri dari uji toksisitas menggunakan metode eksperimental dengan Rancangan Acak Kelompok (RAK) dengan 6 perlakuan 4 kali ulangan dan dilanjutkan dengan analisis sifat campuran. Uji toksisitas dilakukan dengan menggunakan minyak biji nimba tunggal dan campuran minyak biji nimba dengan minyak wijen,  konsentrasi minyak biji nimba tunggal yang digunakan sebesar 0,5%; 0,9%; 1,7%; 3,2%; 6%; dan Kontrol, lalu konsentrasi campuran minyak biji nimba dengan minyak wijen 0,5%; 0,8%; 1,4%; 2,4%; 4%; dengan perbandingan 1:1. Hasil uji toksisitas menunjukkan campuran minyak bersifat toksik dengan LC50 dan LC95 masing-masing sebesar 1,102% dan 2,416% pada 16 Hari Setelah Perlakuan (HSP). Campuran minyak biji nimba dengan minyak wijen dapat memberikan dampak lebih baik dalam menimbulkan mortalitas pada hama S.frugiperda. Hasil analisis sifat campuran minyak biji nimba dengan minyak wijen mendapatkan nilai nisbah sinergistik 2,402, dari hasil tersebut menunjukkan minyak wijen dapat bersinergis dengan minyak biji nimba.
Kombinasi Mikrob Antagonis dan Air Rendaman Kompos untuk Menekan Penyakit Bercak Cokelat pada Daun Tomat Istifadah, Noor; Muslim, Azis; Widiantini, Fitri; Hartati, Sri
Agrikultura Vol 36, No 3 (2025): Desember, 2025
Publisher : Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/agrikultura.v36i3.68219

Abstract

Penyakit bercak cokelat yang disebabkan jamur Alternaria solani merupakan salah satu kendala dalam budidaya tanaman tomat. Pengendalian penyakit secara ramah lingkungan dapat dilakukan melalui aplikasi mikrob pengendali penyakit dan penggunaan bahan organik. Artikel ini membahas hasil penelitian yang mengevaluasi efek pencampuran mikrob antagonis (isolat bakteri dan khamir) serta kombinasi mikrob dengan air rendaman kompos terhadap kemampuannya dalam menekan penyakit bercak cokelat pada daun tomat. Selain pengujian pada tanaman tomat, dilakukan juga percobaan secara in vitro untuk mengevaluasi kompatibilitas dan pengaruh campuran mikrob terhadap kemampuannya dalam menghambat pertumbuhan jamur A. solani. Percobaan secara in vitro menggunakan Rancangan Acak Lengkap dengan lima ulangan, sedangkan percobaan pada tanaman tomat menggunakan Rancangan Acak Kelompok dengan tiga ulangan. Hasil percobaan menunjukkan bahwa bakteri isolat KSB4 (Bacilus subtilis) dan khamir isolat KDK11 asal air rendaman kompos bersifat kompatibel dan dapat menghambat pertumbuhan jamur A. solani secara in vitro sebesar 57,50%-65,23%. Kemampuan isolat mikrob secara tunggal atau campurannya tidak berbeda secara nyata. Aplikasi isolat mikrob antagonis secara tunggal, campuran, air rendaman kompos maupun kombinasinya dapat menekan penyakit bercak cokelat pada tanaman tomat sebesar 78,77%-97,12%.  Pencampuran isolat mikrob antagonis atau kombinasi mikrob antagonis dengan air rendaman kompos tidak menghasilkan penekanan penyakit bercak coklat yang lebih baik daripada perlakuan secara tunggalnya.
Co-Authors Agus Susanto Ai Melawati Anggi Ayuningtyas Anne Nuraini Anne Nurbaity Aoliya, Salwa Rohmatul Arisaputri, Annisa Azis Muslim, Azis Baharudin, Aziz Ceppy Nasahi Danar Dono Dian E. Kkartiwa Diyan Herdiyantoro Endah Yulia Erni Suminar Farida Fitri Widiantini Hersanti - Hilda Sandra Utami I P Sari Kusumiyati Lia Herawati Luciana Djaya Luciana Djaya, Luciana Maharani, Febry Aulia Riski Maulana, Raihan Rahmat Mochamad Arief Soleh Muhamad Salman Umar Nasrul Hakim Noladhi Wicaksana Nurdiana, Dadi Nurjihan, Khansa Nurul Ihsani Octa Saktianti P. Rizky D. Sianipar Prabowo, Yudhisa Hendri Putu Ghita Novilaressa Rahmat Budiarto Retno Anjani Putri Rika Meliansyah Rohmah, Nanda Dea Nikmatu Saleh, Teddy Wahyana Salsabila, Haifanisa Santa Monica Septiandini, Adelia SIska Rasiska, SIska Sri Ageng Fitria Sri Hartati Sri Hartati Sri Hartati Sri Hartati Sri Rosmala Dewi Sudarjat Sudarjat Suseno Amien Syariful Mubarok Tarkus Suganda Toto Sunarto Tri Mayanti Utari, Elinda Dwi Wahyu Daradjat Natawigena, Wahyu Daradjat Wawan Sutari