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All Journal HAYATI Journal of Biosciences Jurnal Ilmu Pertanian Indonesia Jurnal Ilmu Dasar BERKALA SAINSTEK Abdimas REKAYASA UNEJ e-Proceeding BIOLINK (Jurnal Biologi Lingkungan, Industri, Kesehatan) Caraka Tani: Journal of Sustainable Agriculture BIOEDUKASI JPSCR : Journal of Pharmaceutical Science and Clinical Research Abdimas Umtas : Jurnal Pengabdian kepada Masyarakat SULUH: Jurnal Abdimas Journal of Natural Sciences Makara Journal of Science InSTEM Jurnal Malikussaleh Mengabdi Life Science and Biotechnology Biosaintifika: Journal of Biology & Biology Education Jurnal Penelitian Sains dan Teknologi Indonesia
Dwi Setyati
Jurusan Biologi, Fakultas Matematika Dan Ilmu Pengetahuan Alam, Universitas Jember
Religion Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Control & Systems Engineering Earth & Planetary Sciences Economics, Econometrics & Finance Education Electrical & Electronics Engineering Engineering Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Library & Information Science Mathematics Physics Public Health Social Sciences Other

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DNA barcode characteristics of Paphiopedilum supardii Braem & Loeb Mukhamad Su'udi; Vita Sindiya; Tri Ratnasari; Dhiyaul Kholis; Dwi Setyati; Husnatun Nihayah
Jurnal Ilmu Pertanian Indonesia Vol. 31 No. 3 (2026): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18343/jipi.31.3.571

Abstract

Paphiopedilum is a protected orchid (Orchidaceae) genus with the most endangered species due to its unique aesthetic qualities, totaling 15 species. The excessive exploitation of orchids has led to a decline in their natural populations. This has prompted comprehensive conservation efforts to preserve these species. However, it is quite challenging to identify orchids that have not yet bloomed because of the similar morphology of their vegetation among closely related species, necessitating alternative identification approaches that are both rapid and accurate, especially for endangered orchids. DNA barcoding is a rapid and accurate molecular method for species identification. In this study, the selected rare orchid was Paphiopedilum supardii Braem & Loeb. The research procedure included DNA sample isolation using a NEXprepTM Plant DNA Mini Kit, DNA amplification using rbcL and ITS primers, PCR product purification, and sequencing. Based on the research results, DNA samples were amplified using rbcL and ITS primers. The DNA band resulting from rbcL primer amplification was 600 bp in size, whereas the ITS amplification result was 900 bp. The PCR products were then purified directly using a Purification Kit (Jena Bioscience). The PCR and purification results were separated on a 1.25% agarose gel. Based on data analysis, BLAST results from NCBI and sequence alignment using MEGA11, Clustal X2, and GeneDoc showed that the rbcL sequence had a higher homology level than ITS. Therefore, the ITS sequence is more specific for use as a molecular marker in DNA barcoding research on P. supardii. Keywords: ITS, Molecular identification, P. supardii, rbcL
Effect of Colchicine and Bio-catharantin on the DNA Relative Content and Stomatal Structure of Black Rice (Oryza sativa L. var. Jeliteng) Setyati, Dwi; Su’udi, Mukhamad; Wulandari, Dyah Retno; Handoyo, Tri; Ulum, Fuad Bahrul
Caraka Tani: Journal of Sustainable Agriculture Vol 39, No 2 (2024): October
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/carakatani.v39i2.88279

Abstract

Black rice (Oryza sativa L. var. Jeliteng), known for its health benefits compared to white rice, faces challenges in productivity. Among varieties, this black rice is popular in Indonesia but shows low yield. Research on improving black rice through genetic manipulation with antimitotic substances is limited. Therefore, this study aims to compare the effects of colchicine and Bio-catharantin on the germination rate, DNA relative content, and stomatal structure of O. sativa L. var. Jeliteng. Seeds were treated with colchicine (0.1%, 0.2%, and 0.3%) and Bio-catharantin (0.1%, 0.2%, 0.3%, 0.4%, and 0.5%) at soaking durations of 12, 24, and 48 hours. Germination was assayed, ploidy was determined using flow cytometry, and stomatal traits, including size and density, were examined microscopically. The results showed that Bio-catharantin did not exhibit any toxic effects on germination rates, whereas colchicine reduced germination starting at 0.2% concentration. Both chemical agents modified the DNA relative content of Jeliteng black rice. Colchicine generally increased stomatal length and width while decreasing stomatal density, with significant changes at 0.3% concentration for 24 hours. Bio-catharantin also altered stomatal traits, enhancing length and width in most cases but significantly reducing density under certain conditions. Bio-catharantin emerged as a promising alternative to colchicine for inducing chromosomal mutations in plants, offering benefits in altered stomatal structures without the toxic effects on germination, compared to colchicine.
Antimicrobial and Phytochemistry study of Liparis resupinata Ridl. from Mount Gumitir, East Java, Indonesia Ulum, Fuad Bahrul; Setyati, Dwi; Rizqoni, Moh. Ikmal Alfi; Su'udi, Mukhamad
JPSCR: Journal of Pharmaceutical Science and Clinical Research Vol 8, No 3 (2023)
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/jpscr.v8i3.70856

Abstract

Liparis resupinata Ridl. is one of the abundant epiphytic orchids in the Mount Gumitir, Jember Regency with potency for phytopharmacy study. Our study focused on the observation of the species for their antimicrobial and metabolite profiles. This study used Gram-positive and Gram-negative bacteria, i.e., S. aureus, S. typhi, and E. coli with an agar diffusion method for antibacterial activity test. The metabolite profile was generated through GC-MS. The methanolic extract of the leaves of L. resupinata showed positive antibacterial activity. The GC-MS data analysis suggested the presence of antimicrobial substances e.g., (-)-loliolide, nonanoic acid, 1,2-ethanediamine, and hydroxy dimethyl furanone.
Co-Authors A.A. Ketut Agung Cahyawan W Adawiyah, Robiyatul Afidah, Yusfi Aini Nurul Ali Murtodo Ana, Daniar Alfi Andiana, Jocelin Anik Suwandari Anik Swandari Ari Satia Nugraha Arif Mohammad Siddiq Arsetyo Rahardianto Arum, Asyifa Yasmin Bambang Sugiharto Desy Lutfianasari Dhiyaul Kholis DWI INDAHNING ROHMAH Dwinianti, Edia Dwinianti, Edia Fitri Dwinianti, Edia Fitria Esti Utarti Eva Tyas Utami Farhan, Abdillah Maulana Fitri, Edia Fuad Bahrul Ulum Gilani, Noor Azizah Hayyinah, Asa Hanifatul Hidayat Teguh Wiyono Khoirunnisa, Gita Ayu Kumalasari, Deris Erlita Luh Putu Ratna Sundari Luthfiah Luthfiah M. Akbar, Salahuddin Miatin Alvin Septianasari Monica Paulina Erizcy Mukhamad Su'udi Mukhamad Su'udi Mukhamad Su'udi Mukhamad Su'udi Mustafidah, Qori'atul Nihayah, Husnatun Ningrum, Asyifa Yasmin Parawita Dewanti Pining Suwardining Tyas Rajamani, Ranjithkumar Ratnasari, Tri Ravitamala, El Shania Rendy Setiawan Rike Oktarianti Rizqoni, Moh Ikmal Alfi Rizqoni, Moh. Ikmal Alfi Robi’atul Adawiyah Rosida, Waki'atil Rosida, Waki’atil Rosyadi, Achmad Roziqoh, Wasiatur Saniyah Fatkhul Alim Sattya Arimurti Sa’adah, Kurnia Su'udi, M. Su'udi, Mukhamad Sulistiyowati, Hari Sutoyo Sutoyo Su’udi, Mukhamad TRI HANDOYO Tri Ratnasari Tri Ratnasari Tri Ratnasari Tri Ratnasari Umiyah Umiyah Veren Yuliana Saputri Vita Sindiya Wardatus Sholeha Wulandari, Dyah Retno