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New Report of Distribution of Entomopathogenic Fungi Aschersonia sp. Infected Whitefly in Bali I PUTU WIRYA SUPUTRA; I PUTU SUDIARTA; GUSTI NGURAH ALIT SUSANTA WIRYA; I KETUT SUMIARTHA
Agrotrop : Journal on Agriculture Science Vol 9 No 2 (2019)
Publisher : Fakultas Pertanian Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (331.94 KB) | DOI: 10.24843/AJoAS.2019.v09.i02.p09

Abstract

Laporan Baru tentang Distribusi Jamur Entomopatogenik Aschersonia sp. yang Menginfeksi Kutu Putih di Bali. Penelitian tentang distribusi jamur entomopatogenik Aschersonia sp. dilakukan untuk mengetahui persebaran Aschersonia yang menginfeksi kutu putih di seluruh Bali. Penelitian tersebut dilakukan di Kabupaten Gianyar, Bangli dan Buleleng pada centra perkebunan jeruk dimulai tahun 2014 sampai tahun 2018. Aschersonia pertama kali ditemukan pada tahun 2014 di Desa Sekaan Kintamani Bangli dan Desa Kerta Payangan Kabupaten Gianyar, dan pada tahun 2018 ditemukan di Desa Gobleg Sukasada Kabupaten Buleleng. Distribusi jamur tersebut ditemukan di dataran tinggi dan tidak ditemukan di dataran rendah. Hal ini sangat terkait dengan keadaan iklim terutama kelembaban dan suhu. Jamur Aschersonia sangat baik tumbuh pada suhu 20°C yang berada pada dataran tinggi.
The Observation of Blast Disease and Its Effect to Rice Yield Using Existing Assessment Method to Support the Indonesian Agriculture Insurance I Putu Sudiarta; I Komang Candra Giri Prayoga; I Gede Rai Maya Temaja; Gusti Ngurah Alit Susanta Wirya; Masahiro Shishido; Chiharu Hongo
SOCA: Jurnal Sosial Ekonomi Pertanian Vol 15 No 2 (2021): Vol.15, No.2, 2021
Publisher : Program Studi Agribisnis, Fakultas Pertanian, Universitas Udayana Jalan PB.Sudirman Denpasar, Bali, Indonesia. Telp: (0361) 223544 Email: soca@unud.ac.id

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/SOCA.2021.v15.i02.p15

Abstract

One of the causes for the low productivity of rice in Indonesia is the occurrence of rice blast disease. Blast disease is one of the main diseases of rice plants around the world. Therefore to minimise the risk of the lost yield by blast disease, the Ministry of Agriculture of the Republic of Indonesia has the agriculture insurance program. To support these program, the research about observations of blast disease in a fixed location are needed. The objective this research is to confirm pathogens causing the blast disease in the fields, to determine the development of blast disease and its effect to the rice yield (grain weight and straw weight). The study was conducted in a fixed paddy field at Subak Uma Dalem, Sobangan Village, Mengwi District, Badung Regency, Bali Province from February 2019 to April 2019. The data showed that the disease incidence of rice blast increased every week starting from 1st week (28 DAT) until 10th week (104 ATP). Our study confirmed that the fungus caused the blast disease in the field was Magnaporthe oryzae. The higher incidence of blast disease in the field resulted reduced the rice yield by decreasing both grain and straw weights.
The Existing Method to Assess the Disease Incidence of Bacteria Leaf Blight and Its Effect to Rice Yield to Support the Indonesian Agriculture Insurance Gusti Ngurah Alit Susanta Wirya; Made Satya Andrayuga; I Putu Sudiarta; Masahiro Shishido; Chiharu Hongo
SOCA: Jurnal Sosial Ekonomi Pertanian Vol 15 No 3 (2021): Vol.15, No.3, 2021
Publisher : Program Studi Agribisnis, Fakultas Pertanian, Universitas Udayana Jalan PB.Sudirman Denpasar, Bali, Indonesia. Telp: (0361) 223544 Email: soca@unud.ac.id

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/SOCA.2021.v15.i03.p19

Abstract

Bacterial leaf blight (BLB) has become a major obstacle and a limiting factor in national rice production. Basically, this disease is caused by the bacterium Xanthomonas oryzae pv. oryzae. Therefore, to minimize the risk of disease infection to yield lost; The Ministry of Agriculture of the Republic of Indonesia has the agriculture insurance program. To support the program, the observations of disease incidence of the disease using existing method are needed. The purpose of this study was to determine the development of bacterial leaf blight on rice plants in Subak Munggu and to determine the effect of bacterial leaf blight on the yield of rice plants. Research activities included (1) isolation of pathogens from symptomatic leaves of rice plants, (2) weekly observations (3) morphological identification of pathogens (4) yields analysis. The results of this study showed the development of bacterial leaf blight in Subak Munggu, Munggu Village, Mengwi Sub-district, Badung Regency for ten weeks. The yield by calculating the weight of grains in each plot was calculated, it can be concluded that a sample that showed a high percentage of disease at the harvest time will cause the low grain yield production.
KUALITAS AIR PAMSIMAS DESA SABA KABUPATEN GIANYAR DITINJAU DARI SIFAT FISIK, KIMIA DAN MIKROBIOLOGI Ni Wayan Korniasih; I Putu Sudiartawan; Ni Luh Gede Sudaryati
JURNAL WIDYA BIOLOGI Vol 12 No 02 (2021): Widya Biologi
Publisher : UNHI Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32795/widyabiologi.v12i02.2146

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Pemeriksaan terhadap PAMSIMAS Desa Saba di beberapa titik sambungan oleh UPTD Laboratorium Kesehatan Kabupaten Gianyar tahun 2019 melaporkan bahwa bak penampungan air PAMSIMAS di Banjar Sema, Desa Saba telah mengalami pencemaran. Maka peneliti melakukan penelitian kualitas air PAMSIMAS di Desa Saba Kabupaten Gianyar ditinjau dari sifat fisik, kimia dan mikrobiologi. Penelitian ini bertujuan untuk mengetahui kualitas air PAMSIMAS di Desa Saba, Kabupaten Gianyar. Data penelitian dianalisis secara deskriptif komparatif mengacu pada Peraturan Menteri Kesehatan Republik Indonesia Nomor 32 Tahun 2017 dan Peraturan Gubernur Bali No.16 tahun 2016. Pengambilan sampel dilakukan sebanyak 2 (dua) kali di PAMSIMAS Desa Saba, Kabupaten Gianyar dengan jumlah sampel masing-masing pada setiap pengambilan sebanyak 7 (tujuh) sampel (7 stasiun Penelitian), sehingga jumlah sampel dalam penelitian ini adalah 14 (empat belas) sampel. Sedangkan parameter kualitas air yang diteliti sebanyak 15 (lima belas) parameter yang meliputi parameter fisik, kimia dan mikrobiologi. Dari 15 (lima belas) parameter yang diteliti, menunjukkan bahwa terdapat 3 (tiga) parameter kualitas air yang melampaui standar baku mutu lingkungan yaitu parameter BOD, COD dan Escherichia coli dengan nilai rata-rata BOD, COD dan Escherichia coli berturut-turut 9,48 mg/L; 17,41 mg/L dan 3,64 MPN/100 mL. Kualitas air PAMSIMAS Desa Saba, Kabupaten Gianyar memenuhi syarat jika ditinjau dari sifat fisik tetapi dari sifat kimia dan mikrobiologi kualitas air PAMSIMAS Desa Saba tidak memenuhi syarat sebagai bahan baku air minum.
Cell Wall Lytic Enzymes And Their Role In Bacteriophages Infection I Putu Sudiarta; Gusti Ngurah Alit Susanta Wirya
AGRICA Vol. 4 No. 1 (2011): June
Publisher : Agriculture Faculty of Flores University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37478/agr.v4i1.466

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Use of chemical pesticides has been shown to have many negative side effects, such as insecticide resistance and resurgence, an outbreak of secondary pests and diseases, the disappearance of parasitoid and predator species as well as residual effects on food crops and on the environment. Over the past 60 years, both the number of agricultural toxins in the environment and incidence rates of toxin-related diseases has increased dramatically. The most effective way to combat this problem is through the use of natural predators. One of the best examples of this is the use of host-specific bacteriophages to control bacterial diseases. The mechanism of infection is a very interesting one. To break through the bacterial cell wall the bacteriophages must produce a range of lytic enzymes. This review will examine and discuss studies of these site-specific cell wall lytic enzymes and their roles in the infection of bacteriophages.
PERANAN PESTISIDA BOTANI DALAM MENDUKUNG PERTANIAN ORGANIK I Putu Sudiarta
AGRICA Vol. 3 No. 1 (2010): June
Publisher : Agriculture Faculty of Flores University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37478/agr.v3i1.494

Abstract

Until recently the use of synthetic pesticides to control pest and plant diseases proved effective, but on the other hand, the excessive use of synthetic pesticides cause many negative effects, such as the development of pest and disease resistance, second pest explosion, death of natural enemies and pesticide residues in food and environment. One alternative that can be done to solve this problem is the use of botanical pesticides. Botanical pesticides are compounds produced as a plant defence response to disturbances and stimulation. These compounds generally are of secondary metabolites that have many functions, such as growth hormones (Auxin, gibberellins and cytokinins), anti-fungal or anti-bacterial, antibiotics, and toxic to animals and insects. The advantage of botanical pesticides is that they have toxicity similar to synthetic pesticides, but the botanical pesticides can be used to support sustainable organic agriculture. Experiments in the utilization of botanical pesticides to control plant pest have been carried out. One example application is the use of botanical insecticides from “Brotowali” leaf extract (Tinospora crispa) to control the diamond black caterpillars (Plutella xylostella) on cabbage plants. In addition, the use of ‘basil’ oil (Ocimum tenuiflorum) has a real impact in population control of the fruit fly (Bactrocera dorsalis). In addition to pest control, botanical pesticides are also reported to effectively control plant diseases. Use of ‘galangal’ (Alpinia galanga) and papaya (Carica papaya) has a high ability to inhibit the growth of Ceratocystis sp. On PDA and fruits. The combination of ‘betel’ leaf extract (Piper betle) and ‘galangal’ (A galanga) can suppress the growth of banan wilt disease caused by Fusarium oxysporum and / or the Ralstonia solanacearum. The use of ‘galangal’ (A. Galanga) extract with a concentration of 5%, can also inhibit the growth of stem rot disease (F. oxisporum) on vanilla seedlings. In addition, the use of ‘betel’ ieaf P. Bettle in the field can suppress black rot disease on cocoa pods (cocoa black pd disease)
MtCOI DNA sequences from Sycanus aurantiacus (Hemiptera: Heteroptera: Reduviidae) provide evidence of a possible new harpactorine species from Bali, Indonesia I Putu Sudiarta; Dewa Gede Wiryangga Selangga; Gusti Ngurah Alit Susanta Wirya; Ketut Ayu Yuliadhi; I Wayan Susila; Ketut Sumiartha
Jurnal Entomologi Indonesia Vol 18 No 1 (2021): Maret
Publisher : Perhimpunan Entomologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5994/jei.18.1.74

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Sycanus aurantiacus Ishikawa & Okajima, found in Bali, was first described in 2007 as a new harpactorine species based on morphological and biological characteristics; however, its genome has not yet been sequenced. In this study, we examine the mitochondrial cytochrome oxidase subunit I (MtCOI) nucleotide sequence of S. aurantiacus in order to determine whether it represents a new harpactorine species. A sample from Pancasari, Bali, Indonesia was collected at the same location S. aurantiacus was first discovered in 2007. The selected mtCOI gene (650 bp) was successfully amplified using mtCOI primer pairs LCO1490 and HCO2198, and the resulting MtCOI sequence of the S. aurantiacus sample was compared with those from other hapactorine species recorded in GenBank. This comparison revealed low genetic similarity between S. aurantiacus and most other harpactorine species worldwide, except for the Genus Sycanus (JQ888697) from USA whose mtCOI shares approximately 91% similarity with the Pancasari sample. Phylogenetic analysis indicated a close genetic relationship between Sycanus from Bali and the Genus Sycanus (JQ888697) from the USA. The mtCOI sequence of S. aurantiacus had not been recorded previously, and our comparison with existing Sycanus sequences provides support to the understanding that S. aurantiacus is indeed its own species.
Influence of Some Packages of Technology on Pests Development on Chili Plants in Highland Area I Putu Sudiarta; Luciana Delavega; Ni Nengah Darmiati; Gusti Ngurah Alit Susanta Wirya; I Ketut Sumiartha; I Made Supartha Utama
Journal of Sustainable Development Science Vol 1 No 1 (2019)
Publisher : Dwijendra University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (297.819 KB) | DOI: 10.46650/jsds.1.1.791.8-14

Abstract

Chili is a type of plant that can grow in various types of area, in lowland and highland areas and also can adapt well at a temperature of 24-27 ˚C. The purpose of this research in order to know the influence and effectiveness of the packages technology of Trichoderma sp. mixed with compost, rain shelter and pesticides to control the development of pests in cayenne chili plants and long chili in the highland area. This research was conducted from September 2017 to February 2018, in Pancasari Village, Buleleng Regency, Bali. The study used a Factorial Randomized Block Design with two factors and four times replications. The first factor was chili type (cayenne chili and long chilli), and the second factor was the use of technologies, compost plus Trichoderma sp., rain shelter, and pesticide. The variable was observed in this research were population of aphis, percentage of fruit fly and yields. The results showed that all treatment technologies effectively suppressed pests development when compared with control. The result show that the damage population of the highest aphis pest was found in control about 194 of Aphids, and population of aphids on pesticide treatment were not found. The result show that damage percentage of the highest fruit fly pest was found in control treatment with the percentage of 76,55% while the lowest percentage was found in pecticide treatment. The rain shelter treatment showed the highest yields were reached 405,87 gr and the control treatment showed the lowest yields were reached 12,03 gr.
UTILIZATION OF THE FLOWER OF Tagetes Erecta LINN AS A REPELLENT AGAINST Aedes Aegypti L (DIPTERA: CULIDAE) MOSQUITO Ni Putu Noviyanti; I Putu Sudiarta; Anak Agung Ayu Agung Sri Sunari
International Journal of Biosciences and Biotechnology Vol 9 No 2 (2022): INTERNATIONAL JOURNAL OF BIOSCIENCES AND BIOTECHNOLOGY
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/IJBB.2022.v09.i02.p04

Abstract

The use of Tagetes Erecta Linn flowers as a repellent against the Aedes Aegypti L mosquito (Diptera: culidae) has been carried out. Extraction of 570 grams of Tagetes flower samples was carried out by maceration using methanol to produce 44 grams of thick green extract. Phytochemical test results showed the methanol extract of Tagetes flower samples were positive for flavonoids. Compound fractionation was carried out and obtained 3 fractions, namely water fraction, acetone fraction and n-hexane. The results of the phytochemical test showed that the n-hexane fraction was negative for flavonoids, while the water and acetone fractions were positive for flavonoids. The total yield of flavonoids in the water and acetone fractions was 8293.2692 mg/100 grams and 4964.1148 mg/100 grams, respectively. The water fraction of Tagetes flower extract was tested for active compounds using the GC-MS instrument. Test the effectiveness of the repellent effectiveness of Tagetes flower compounds, the lowest was 5% at 5 hours at 9.35% and the highest was 20% at 1 hour at 60.58%.
Modeling the Incidence of Yellowing Diseases to Whiteflies and Alternative Host Gusde Ida Bagus Gde Pranatayana; I Putu Sudiarta; Ali Nurmasyah
SEAS (Sustainable Environment Agricultural Science) Vol. 5 No. 2 (2021)
Publisher : Warmadewa University Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (231.043 KB) | DOI: 10.22225/seas.5.2.3936.88-95

Abstract

Yellowing diseases is one types of plant diseases which are found in some of the centers of cultivation of yard long bean in Bali. The spread of yellowing diseases caused the presence of vector insects, such as whiteflies and alternative host plants, such as weeds and other crops. This problem is then examined through statistical modeling in order to determine the pattern of the relationship between the incidence of yellowing disease with the populations of whiteflies and the alternative host plants. Collection of data on the yellowing disease incidences, the abundance of whiteflies, and the existence of alternative host plants was done through a direct observation in 100 farmer fields in several centers of cultivation of yard long beans in Bali. Determination of the best mathematical model was performed by linear regression analysis with ordinary least squares method. The results indicated that the best models for the relationship between the incidence of yellowing disease (KPK) with the population of whiteflies (KB) as well as the alternative host plants of yellowing were as follows: KPK = 1,2265 KB0,9872 TIAK1,0936 (R2 = 98,43 %; P-value = 0,000). The incidence of yellowing disease was highly significant positively correlated with the population of whiteflies and yellowing symptomatic alternative host plants. The existence of alternative host plants around the field was very decisive in triggering the emergence of yellow virus disease in the crop.
Co-Authors - Hamid Agung, I Dewa Agung Putra AGUS PUTRA SEDANA Ali Nurmasyah Americo Alves Brito Anak Agung Ayu Agung Sri Sunari Anak Agung Sagung Kendran ANANDA RIZKI NURANI ARIYANTA, I PUTU BAWA Chiharu Hongo Damastra, Garda Bagus Desak Putu Sudarmini Devi, Komang Saraswati Devi, Putu Shinta DEWA AYU DIAH PUSPITASARI Dewa Gede Wiryangga Selangga Dewa Gede Wiryangga Selangga Dewa Gede Wiryangga Selangga DINA RAHMAWATI Dinarkaya, Shah Mahapati Dwi Martiningsia Dwi Martiningsia DWI SUGIARTA DWI WIDANINGSIH Eka Wijayanti, Febri G.A.M.K. Dewi GARGITA, I WAYAN DIKSA Gede Suastika Gede Suastika GREG LUTHER GREGORY C. LUTHER Gusti Ayu Mayani Kristina Dewi Gusti Ayu Yuniati Kencana Gusti Ngurah Alit Susanta Wirya GUSTI NGURAH GEDE DHARMA PUTRA PUTRA Hamid, - Harianja, Shara Yulita Hartha , I Komang Gede Suweca I DEWA MADE PUTRA WIRATAMA I Dewa Nyoman Nyana I DEWA PUTU SINGARSA I G. R. M. TEMAJA I G. Wijana I GEDE PUTU WIRAWAN I GEDE SILA ADNYANA I Gst Agung Putu Raka Agung I GUSTI AYU DEVI VALENIA SARI I Gusti Ngurah Bagus I Gusti Ngurah Kade Mahardika I Ketut Mangku Budiasa I KETUT PURNA YASA I KETUT SUMIARTHA I Komang Candra Giri Prayoga I KOMANG VIDIA DHARMA TARO PUTRA I Made Arimbawa I Made Dira Swantara I Made Sudana I MADE SUDARMA I Made Sukewijaya I MADE SUPARTHA UTAMA I MADE WINANTARA I MADE YESTA SANTIATMA I NYOMAN DARMA YASA I NYOMAN MANTIK ASTAWA I Nyoman Suarsana I Nyoman Suartha I NYOMAN WIJAYA I PUTU BAWA ARIYANTA I PUTU DHARMA I PUTU WIRYA SUPUTRA I Wayan Arta Wijaya I WAYAN RUSMAN I Wayan Suardana I Wayan Supartha I Wayan Susila I.A.P. Apsari I.B.K. Suardana Ida Ayu Pasti Apsari IDA BAGUS GDE PRANATAYANA Ida Bagus Kade Suardana JOKO MARIYONO JOKO MARIYONO KETUT AYU YULIADHI Klett, Katrina Listihani, Listihani Luciana Delavega LUTFI SURYAWAN M SUDANA Made Getas Pudak Wangi MADE MIKA MEGA ASTUTHI Made Mika Mega Astuthi Made Satya Andrayuga MARIA ULFA ELLA ELLA Masahiro Shishido MOCH BINTANG RAMADHAN NI KADEK NINA ARI SUCI Ni Kadek Nita Karlina Astriyani Ni Luh Gede Sudaryati NI LUH WAHYU SUTARINI, NI LUH WAHYU NI MADE DWI ANTARI Ni Made Dwi Desiyanti Ni Made Puspawati NI MADE SAVITA RASJMAN NI MADE WINDA UTARI NI NENGAH DARMIATI Ni Putu Merthaningsih Ni Putu Noviyanti Ni Wayan Korniasih NI WAYAN SUNITI NYOMAN SEMADI ANTARA Putra, I Gusti Putu Semara Putu Perdana Kusuma Wiguna R. SRINIVASAN RAMADHAN, MOCH BINTANG S.K. Widyastuti SANJAYA, I GUSTI NGURAH PRABU WIRA Selangga, Dewa Gede Wiryangga SONIA ASHA HASARI Sri Kayati Widyastuti SUGIARTA, DWI Sumartayasa, I Wayan Andi SUNARI, ANAK AGUNG AYU AGUNG SRI Suputra , I Putu Wirya SUPUTRA, I PUTU WIRYA Syahbana , Raditya Darmawan TRISNA AGUNG PHABIOLA TRISNA AGUNG PHABIOLA Yuliadhi , Ketut Ayu Zhao, Tiejun