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All Journal Agrotrop : Journal on Agriculture Science SOCA: Jurnal Sosial Ekonomi Pertanian Jurnal Kimia (Journal of Chemistry) International Journal of Biosciences and Biotechnology Buletin Udayana Mengabdi Jurnal SPEKTRUM Journal of Agricultural Science and Biotechnology E-Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Jurnal Ekonomi dan Bisnis Jagaditha Agrovigor: Jurnal Agroekoteknologi Jurnal Perlindungan Tanaman Indonesia Journal of Tropical Biodiversity and Biotechnology JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA AGROMIX Jurnal Entomologi Indonesia Jurnal Widya Biologi Agrica: Journal of Sustainable Dryland Agriculture SEAS (Sustainable Environment Agricultural Science) Journal of Sustainable Development Science
I Putu Sudiarta
Magister Of Agricultural Biotechnology, Faculty Of Agriculture Of Udayana University, Jl. PB. Sudirman, Denpasar 80231 Bali
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Economics, Econometrics & Finance Education Electrical & Electronics Engineering Energy Engineering Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Medicine & Pharmacology Public Health

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Metode Isolasi Jamur Patogen Serangga (Aschersonia placenta) Menggunakan Media Water Agar dan Potato Sucrose Agar NI MADE SAVITA RASJMAN; I PUTU SUDIARTA; TRISNA AGUNG PHABIOLA; GUSTI NGURAH ALIT SUSANTA WIRYA; I PUTU WIRYA SUPUTRA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.11, No.2, April 2022
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Isolation Method of Insect Pathogenic Fungi (Aschersonia placenta) using Water Agar Media and Potato Sucrose Agar Bali Province is one of the provinces that is famous for its superior agricultural commodities. One of the leading agricultural commodities is citrus. The production of citrus fruits in Bali Province in 2020 is 4,903,341 quintals based on data from the Central Statistics Agency for Bali Province 2021. However, in some citrus-producing areas, citrus fruit production has decreased compared to the previous year. The decline in citrus fruit production is caused by several factors, one of which is the attack of whitefly pests. Whitefly pests can be controlled by utilizing the insect pathogenic fungus Aschersonia placenta. This study aims to determine the isolation method of insect pathogenic fungi Aschersonia placenta using water agar media and potato sucrose agar. The study began with sampling, isolated the fungus and identifying the fungus by morphological characteristics. The results of this study showed that isolation method of A. placenta using water agar media and potato sucrose agar effective for multiply the fungus.
A new report of moss-camouflaging mantis Nanomantinae in Bali, Indonesia Sudiarta, I Putu; Wirya, Gusti Ngurah Alit Susanta; Selangga, Dewa Gede Wiryangga; Sanjaya, I Gusti Ngurah Prabu Wira; Arimbawa, I Made; Damastra, Garda Bagus
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 23 No. 2 (2023): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA: JOURNAL OF TROPICAL PLAN
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jhptt.2231-6

Abstract

In 2019 we found moss praying mantis around the forest in the Bedugul Area of Bali, Indonesia. This species belongs to the family Mantidae, subfamily Nanomantinae, and predicted as member of  Calofulcinia genus based on diagnostics of morphological characteristics, behavior, and habitat. Information about the morphological characteristics and data from the molecular sequencing database of the genus Calofulcinia has been very limited. In this research, the barcoding DNA of mitochondrial Cytochrome Oxidase I (mtCOI) of the species is noted using paired primers LCO1490 and HCO2198. Based on the sequence analysis (identity matrix and phylogeny analysis), the moss mantis from Bali has low similarity and genetic relationship with other Mantodea species from GenBank database. The data indicate the moss mantis from Bali has not been reported previously. In addition, the distribution of moss mantis species in Bedugul, Bali, Indonesia has just been described and recorded.
First Report of Cladosporium dominicanum Zalar, de Hoog & Gunde-Cim. Infecting Whitefly on Ornamental Plants in Bali, Indonesia Sudiarta, I Putu; Sugiarta, Dwi; Selangga, Dewa Gede Wiryangga; Wirya, Gusti Ngurah Alit Susanta; Gargita, I Wayan Diksa; Yuliadhi, Ketut Ayu; Klett, Katrina
Journal of Tropical Biodiversity and Biotechnology Vol 9, No 2 (2024): June
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.86865

Abstract

As natural enemies, entomopathogenic fungi are essential for controlling certain plant pests, such as whitefly. Many types of entomopathogenic fungi can infect whiteflies, including those from the genera Aschersonia, Metarhizium, Beauveria, and Cladosporium. Currently, there is great interest in using entomopathogenic fungi as an environmentally friendly pest control in organic and sustainable agricultural systems. This study aimed to identify entomopathogenic fungus associated with whiteflies. Identification of entomopathogenic fungus was performed morphologically and molecularly using DNA barcoding with ITS-1 and ITS-4 as primers, in addition to sequencing and phylogenetic tree analysis. Whiteflies infected with entomopathogenic fungus were discovered on ornamental plants (Premna serratifolia, Ficus religiosa, and Ficus rumphii) in Denpasar, Bali, Indonesia from January to March 2023. We found that samples from the field had mycelium that was unbranched or sparingly branched with solitary conidiophores arising terminally from ascending hyphae or laterally from plagiotropous hyphae. Samples from PDA media had ramoconidia with darkened septa, straight and conidia in long branched chains, branching in all directions, conidia were obovoid, ovoid to limoniform. The fungal colonies had the characteristics of being grey-olivaceous, olivaceous-black, and the margins were grey-livaceous to white, gray olivaceous in colony center due to abundant sporulation. In addition, based on the BLAST nucleotide sequence from NCBI GenBank, the insect pathogen phylogenetic tree found in Denpasar, Bali, is in the same clade as Cladosporium dominicanum strain SCAU014, accession number KY827344.1. Based on morphological and molecular analysis the fungus that infects whiteflies on ornamental plants is C. dominicanum.
Occurrence of Cassava Lace Bug Vatiga illudens (Drake, 1922) (Hemiptera: Heteroptera: Tingidae) in Bali, Indonesia Sudiarta, I Putu; Dinarkaya, Shah Mahapati; Devi, Komang Saraswati; Ariyanta, I Putu Bawa; Wirya, Gusti Ngurah Alit Susanta; Sugiarta, Dwi; Selangga, Dewa Gede Wiryangga; Gargita, I Wayan Diksa; Wiguna, Putu Perdana Kusuma; Yuliadhi, Ketut Ayu; Devi, Putu Shinta
Journal of Tropical Biodiversity and Biotechnology Vol 9, No 1 (2024): March
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.87438

Abstract

Cassava Lace Bugs (CLB) are native pest of cassava (Manihot esculenta Crantz, Euphorbiaceae) to the Neotropical Region, mainly in Brazil. On the other hand, East Java was the first region in Indonesia to record the presence of CLB in 2021, however, it has not been reported in other regions in Indonesia. Therefore, the very importance to recognise the occurrence of CLB in other regions in Indonesia. Based on this, the research has been carried out starting with a field survey, observing behaviour of insect in the field and identify morphologically in the laboratory. The survey results show that the infestation of CLB has been found in lowland, medium, and highland areas in Bali. Symptoms of infestation on the upper leaf surface are small yellow spots with brownish variations. Based on the identification key, CLB from Bali Indonesia, shows a characteristic of a head with a pair of frontal spines. Based on this evidence, the CLB insect can be identified as Vatiga illudens (Hemiptera: Heteroptera: Tingidae). It is the first report of novel distribution areas for V. illudens in Bali, Indonesia. The results of this research are important because V. illudens is one of the main pests of cassava. 
The First Report of the Occurrence of the Root Mealybug Ripersiella multiporifera Jansen (2008) (Hemiptera: Coccoidea: Rhizoecidae) in Indonesia Devi, Komang Saraswati; Sudiarta, I Putu; Sunari, Anak Agung Ayu Agung Sri; Wirya, Gusti Ngurah Alit Susanta; Devi, Putu Shinta; Eka Wijayanti, Febri
Journal of Tropical Biodiversity and Biotechnology Vol 9, No 2 (2024): June
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.89662

Abstract

Ripersiella multiporifera is a root mealybug species within the family Rhizoecidae that has distinctive bitubular pores on the dorsal and ventral. This species was first discovered by Jansen (2008) in the Netherlands during an import interception of Sansevieria sp. from Indonesia and Hoya kerrii from Thailand. This species was also found during inspections in Sicily (Italy) on the roots of Sansevieria trifasciata. In Indonesia, there are no reports of the existence of R. multiporifera, emphasising the need for research on its presence. This work was conducted the morphological method based on modified determination key and the molecular method based on MtCOI gene. The identified species was R. multiporifera, and this finding represents the first evidence of R. multiporifera's presence in Bali (Indonesia) which can be used as a reference for future research, especially in population control approaches. 
Morphological Identification Characteristics of Basil (Ocimum spp.) in Tabanan Regency, Bali, Indonesia Harianja, Shara Yulita; Sudiarta, I Putu; Zhao, Tiejun
International Journal of Biosciences and Biotechnology Vol 11 No 2 (2024): International Journal of Biosciences and Biotechnology
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/IJBB.2024.v11.i02.p07

Abstract

Basil (Ocimum spp.) is an aromatic plant and is the wealthiest essential oil-producing genera from the Lamiaceae family. Due to the various phytochemical compounds or secondary metabolites, Basil has the potential of medicinal plant germplasm as a source of biopharmaceuticals. Several types of Basil grow in Bali, especially in Tabanan Regency, but their morphological characteristics have yet to be identified. This study aims to identify the morphological characteristics of the four types of Basil found growing at that location: Kecarum, Selasih, Tulasi, and Ruku-ruku. This research was conducted by characterizing the characteristics of the parts of the basil plant, including the morphology of the crown (Botany), stem (Caulis), flowers (Flos), leaves (Folium), and fruit (Fructus). The results of the characterization showed that there were similarities between the four types of Basil and differences in crown shape, plant height, the direction of branch growth, type of branching, leaf shape, color (leaves, stems, flowers, fruit), leaf tips, and leaf layout. , and the base of the leaf. Based on the morphological identification, Kecarum and Selasih have similar morphology to Ocimum americanum L and Ocimum basillicum. Tulasi and Ruku-ruku are morphologically identical to Ocimum sanctum var. Rama (red holy basils) and Ocimum campechianum. Keywords: Biopharmaceuticals, Basil, Lamiaceae, Morphology, Ocimum spp.
Inventory of pests attacking Adenium spp. in Denpasar city Gargita, I Wayan Diksa; Sudiarta, I Putu; Yuliadhi, Ketut Ayu; Sumartayasa, I Wayan Andi
Agrovigor Vol 17, No 2 (2024)
Publisher : Universitas Trunojoyo Madura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21107/agrovigor.v17i2.24176

Abstract

The cultivation of Adenium spp. encountered several challenges involving physical, environmental, and plant pest interference factors. Inventory of pests aimed to find out the kind of pests, the symptoms of the attacks, and the percentage of the pests that attack Adenium spp. Samples were taken using a purposive method by taking pictures and observing plants attacked by pests in 4 sub-districts in Denpasar, namely West Denpasar, East Denpasar, South Denpasar, and North Denpasar. The research results show that several pests attack Adenium spp. in Denpasar City, including mealybugs, spider mite, red cotton bug, and aphids. The study's findings indicate that Adenium spp. in Denpasar City are susceptible to several pests, such as mealybugs, spider mite, red cotton bug, and aphids. These organisms induce the dehydration, withering, and stunted growth of plants due to fluid loss. Mealybugs exhibit the highest incidence of infestation, with a prevalence rate of 85%. Spider mites follow with an infestation rate of 50%, while red cotton bug and aphids display infestation rates below 50%. The presence of several pest insects in Adenium spp. is indicative, with certain pests posing a significant threat to the plants, perhaps resulting in severe damage or even plant mortality. This research can provide additional information of pests that attack ornamental plants so that the strategy of pest control on ornamental plants, especially Adenium, can be developed.
Symptoms variation and molecular characterization of Strawberry vein banding virus in Bali, Indonesia Phabiola, Trisna Agung; Selangga, Dewa Gede Wiryangga; Wirya, Gusti Ngurah Alit Susanta; Sudiarta, I Putu; Dinarkaya, Shah Mahapati; Listihani, Listihani
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 25 No. 1 (2025): MARCH, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA: JOURNAL OF TROPICAL PLANT PE
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jhptt.12544-53

Abstract

Vein banding disease has affected young strawberry leaves in Bali over the past five years. Caused by Strawberry vein banding virus (SVBV), the disease is primarily associated with fruit size reduction, though its exact impact remains unclear. This study aims to assess symptom variation, disease severity, and the molecular characteristics of SVBV in Bali strawberry plants. Field observations and molecular identification were conducted using PCR with SVBV-specific primers targeting the CP gene. Samples were collected from ten locations in Bali, including Pancasari, Candikuning, Wanagiri, Gobleg, and Kembang Merta. SVBV DNA from Candikuning, Pancasari, and Kembang Merta was successfully amplified, confirming SVBV infection as the cause of vein banding symptoms. SVBV induces vein banding with upward and downward leaf curling. Disease incidence was highest in Pancasari 1 and 3 (80%) and lowest in Wanagiri 1 and Gobleg (20–28%). Disease severity ranged from 13% to 83%, with the highest recorded in Pancasari, Buleleng, and the lowest in Kembang Merta, Tabanan. Molecular analysis revealed that the SVBV isolate from Bali shares 99.3–100% sequence homology with Chilean isolates. Phylogenetic analysis showed clustering with SVBV isolates from the United States, Brazil, and Chile. This study provides the first molecular characterization of SVBV in Bali, contributing to a better understanding of its epidemiology and potential impact on strawberry production.
The Effect of media types on the growth of insect pathogenic fungi (Aschersonia placenta) Sudiarta, I Putu; Hartha , I Komang Gede Suweca; Yuliadhi , Ketut Ayu; Wirya, Gusti Ngurah Alit Susanta; Selangga, Dewa Gede Wiryangga; Suputra , I Putu Wirya; Listihani, Listihani
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 25 No. 1 (2025): MARCH, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA: JOURNAL OF TROPICAL PLANT PE
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jhptt.12554-62

Abstract

Bali has favorable conditions for the production and expansion of citrus plantations. However, citrus cultivation in Bali often faces challenges, including reduced yield caused by pest infestations and pathogenic diseases. One significant pest affecting citrus plants is the whitefly. A potential method for controlling whiteflies is the use of natural enemies. The entomopathogenic fungus Aschersonia placenta is one of natural enemy that can effectively manage whitefly infestations. However, the succesful utilization of A. placenta requires a specialized approach, particularly in selecting an appropriate growth medium. This study aimed to investigate the impact of different growth media on the development of the entomopathogenic fungus A. placenta. The research began with field sampling, followed by the isolation and morphological identification of A. placenta to obtain a pure culture. Subsequently, media tests were conducted to evaluate fungal growth. Nine treatments were implemented to examine the impact of different media: (1) Water Agar (WA), (2) Potato Dextrose Agar (PDA), (3) Potato Sucrose Agar (PSA), (4) Water Agar + Weaver Ant Eggs Flour (WA-WAEF), (5) Potato Dextrose Agar + Weaver Ant Eggs Flour (PDA -WAEF), (6) Potato Sucrose Agar + Weaver Ant Eggs Flour (PSA-WAEF), (7) Water Agar + Cricket Flour (WA-CF), (8) Potato Dextrose Agar + Cricket Flour (PDA-CF), and (9) Potato Sucrose Agar + Cricket Flour (PSA-CF). The results demonstrated that incorporating cricket insect flour (Gryllidae: Orthoptera) or weaver ant egg flour (Oecophylla smaragdina) into potato sucrose media provided the most optimal growth environment for A. placenta.
Detection and molecular identification of citrus stem rot disease in Bali Province Wirya, Gusti Ngurah Alit Susanta; Agung, I Dewa Agung Putra; Sudiarta, I Putu

Publisher : Fakultas Pertanian Universitas Yudharta Pasuruan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35891/agx.v16i1.4075

Abstract

One major disease of citrus is basal stem rot which may cause significant constraint on citrus production in Indonesia. Basically the diseasecan be caused by fungi Phytophthora spp., Diplodia sp., and Botryodiplodia theobromae or Lasiodiplodia theobromae. The purpose of this study wasto provide information on the main pathogens and characterization of BPB disease, especially in citrus in Bali, which ultimately leads to early warning for protection against BPB disease and enriches academic evidence about pre-existing BPB disease.The method used is observation andisolation, pathogenicity test, and in vitro inhibitory test. Research activities include (1) sampling, (2) isolation of pathogens from symptomatic citrusplants, (3) test of pathogenicity, (4) identification of pathogen morphology and molecular, (5) DNA amplification, (6) DNA electrophoresis (7) analysis of DNA sequences. The results of this research successfully identified Lasiodiplodia theobromae, as a pathogen of citrus stem rot disease in Bali Province.
Co-Authors - Hamid Agung, I Dewa Agung Putra AGUS PUTRA SEDANA Ali Nurmasyah Americo Alves Brito Anak Agung Ayu Agung Sri Sunari Anak Agung Sagung Kendran ANANDA RIZKI NURANI Anu Watu, Getrud Novia ARIYANTA, I PUTU BAWA Chiharu Hongo Damastra, Garda Bagus Desak Putu Sudarmini Devi, Komang Saraswati Devi, Putu Shinta DEWA AYU DIAH PUSPITASARI Dewa Gede Wiryangga Selangga Dewa Gede Wiryangga Selangga Dewa Gede Wiryangga Selangga DINA RAHMAWATI Dinarkaya, Shah Mahapati Dwi Martiningsia Dwi Martiningsia DWI SUGIARTA DWI WIDANINGSIH Eka Wijayanti, Febri G.A.M.K. Dewi GARGITA, I WAYAN DIKSA Gede Suastika Gede Suastika GREG LUTHER GREGORY C. LUTHER Gusti Ayu Mayani Kristina Dewi Gusti Ayu Yuniati Kencana Gusti Ngurah Alit Susanta Wirya GUSTI NGURAH GEDE DHARMA PUTRA PUTRA Hamid, - Hanifah, Wafa’ Nur Harianja, Shara Yulita Hartha , I Komang Gede Suweca I DEWA MADE PUTRA WIRATAMA I Dewa Nyoman Nyana I DEWA PUTU SINGARSA I DEWA PUTU SINGARSA, I DEWA PUTU I G. R. M. TEMAJA I G. Wijana I GEDE PUTU WIRAWAN I GEDE SILA ADNYANA I Gst Agung Putu Raka Agung I GUSTI AYU DEVI VALENIA SARI I Gusti Ngurah Bagus I Gusti Ngurah Kade Mahardika I Ketut Mangku Budiasa I KETUT PURNA YASA I KETUT SUMIARTHA I Komang Candra Giri Prayoga I KOMANG VIDIA DHARMA TARO PUTRA I Made Arimbawa I Made Dira Swantara I Made Sudana I MADE SUDARMA I Made Sukewijaya I MADE SUPARTHA UTAMA I MADE WINANTARA I MADE YESTA SANTIATMA I NYOMAN DARMA YASA I NYOMAN MANTIK ASTAWA I Nyoman Suarsana I Nyoman Suartha I NYOMAN WIJAYA I PUTU BAWA ARIYANTA I PUTU DHARMA I PUTU WIRYA SUPUTRA I Wayan Arta Wijaya I WAYAN RUSMAN I Wayan Suardana I Wayan Supartha I Wayan Susila I.A.P. Apsari I.B.K. Suardana Ida Ayu Pasti Apsari IDA BAGUS GDE PRANATAYANA Ida Bagus Kade Suardana JOKO MARIYONO JOKO MARIYONO KETUT AYU YULIADHI Klett, Katrina Listihani, Listihani Luciana Delavega LUTFI SURYAWAN M SUDANA Made Getas Pudak Wangi MADE MIKA MEGA ASTUTHI Made Mika Mega Astuthi Made Satya Andrayuga MARIA ULFA ELLA ELLA Masahiro Shishido Maulinda, Restiana MOCH BINTANG RAMADHAN NI KADEK NINA ARI SUCI Ni Kadek Nita Karlina Astriyani Ni Luh Gede Sudaryati NI LUH WAHYU SUTARINI, NI LUH WAHYU NI MADE DWI ANTARI Ni Made Dwi Desiyanti Ni Made Puspawati NI MADE SAVITA RASJMAN NI MADE WINDA UTARI NI NENGAH DARMIATI Ni Putu Merthaningsih Ni Putu Noviyanti Ni Wayan Korniasih NI WAYAN SUNITI NYOMAN SEMADI ANTARA Putra, I Gusti Putu Semara Putu Perdana Kusuma Wiguna R. SRINIVASAN RAMADHAN, MOCH BINTANG S.K. Widyastuti SANJAYA, I GUSTI NGURAH PRABU WIRA Selangga, Dewa Gede Wiryangga SONIA ASHA HASARI Sri Kayati Widyastuti SUGIARTA, DWI Sumartayasa, I Wayan Andi SUNARI, ANAK AGUNG AYU AGUNG SRI Suputra , I Putu Wirya SUPUTRA, I PUTU WIRYA Syahbana , Raditya Darmawan TRISNA AGUNG PHABIOLA TRISNA AGUNG PHABIOLA Yuliadhi , Ketut Ayu Zhao, Tiejun