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All Journal Jurnal Ilmu dan Teknologi Kelautan Tropis ILMU KELAUTAN: Indonesian Journal of Marine Sciences BULETIN OSEANOGRAFI MARINA Journal of Oceanography Jurnal Pengolahan dan Bioteknologi Hasil Perikanan Journal of Marine Research Jurnal Kelautan Tropis Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology JRST (Jurnal Riset Sains dan Teknologi) Jurnal Intek Akuakultur Jurnal DISPROTEK Pagaruyuang Law Journal Ulumuddin : Jurnal Ilmu-ilmu Keislaman International Journal of Social and Management Studies (IJOSMAS) Indonesian Journal of Mollusk Mukaddimah: Jurnal Studi Islam Analisa: Journal of Social Science and Religion Journal of Fisheries & Marine Jurnal Ekonomi dan Bisnis Islam

Ali Ridlo

Department Of Marine Sciences, Faculty Of Fisheries And Marine Sciences, Diponegoro University

Author-ID : 2957752

Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Electrical & Electronics Engineering Engineering Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Library & Information Science Social Sciences Other

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Efek Penambahan Gula Terhadap Kestabilan Warna Ekstrak Fikosianin Spirulina sp. Sri Sedjati; Ali Ridlo; Endang Supriyantini
Jurnal Kelautan Tropis Vol 18, No 1 (2015): JURNAL KELAUTAN TROPIS
Publisher : Universitas Diponegoro

Mikroalga Spirulina sp. memiliki kandungan fikosianin yang berpotensi untuk digunakan sebagai pewarna alami, namun fikosianin tidak stabil terhadap suhu, cahaya, pH, dan oksigen, sehingga perlu ditambahkan pengawet. Penelitian ini bertujuan untuk mengetahui pengaruh pengawet gula terhadap kestabilan fikosianin yang diekstraksi dari Spirulina sp. Spirulina sp diekstraksi dengan akuades dan larutan buffer fosfat pH 7. Efek penambahan gula diamati selama 14 hari penyimpanan pada suhu kamar (30±30C). Stabilitas pigmen diamati berdasar nilai konsentrasi relatifnya (CR). Hasil penelitian menunjukkan bahwa kadar fikosianin Spirulina sp. yang diekstraksi dengan aquades adalah 45,16±1,13 mg/g dw, sedangkan untuk ekstrak dengan pelarut buffer fosfat pH 7 adalah 760,51±0,11 mg/g dw. Penambahan gula fruktosa 5% memberikan hasil yang paling efektif selama 14 hari penyimpanan suhu kamar 30±30C, konsentrasi relatif yang tersisa sebesar 19,39 %, sedangkan tanpa pengawet konsentrasi relatif hanya 10,57 %. Secara umum penambahan gula (sukrosa, fruktosa dan glukosa 5% dan 10% b/v) mampu meningkatkan konsentrasi relatif menjadi 12,12-19,39%. Kata kunci : Fikosianin, Spirulina sp, gula, Aktivitas Antioksidan Microalga Spirulina sp. has a high content of phycocyanin. Phycocyanin has potential as a natural blue colorant. Phycocyanin is not stable against temperature, light, pH and oxygent. Preservatives are food additives commonly used to preserve unstable food. The purpose of this research was to screening sugar preservatives for phycocyanin. The effect of selected sugar preservatives, sucrose, glucose and fructose on the stability of phycocyanin at room temperature (30±30C) was studied in aqueous solution for 14 days. Pigment stability after 14 days storages was observed from concentration relative (CR) value. The result showed that phycocyanin content in aquadest extract was 45,16±1,13 mg/g dw. The CR value remained at approximately 12,12-19,39% after storage for 14 days at room temperature when sugar preservatives (sucrose, glucose and fructose 5% and 10% w/v) was added, while without preservatives CR value decreased to 10,57%. The highest stability of the phycocyanin solution was found in adding fructose 5% (CR value = 19,39%). Keywords : Phycocyanin, Spirulina sp., Sugar, Antioxidant Activity

Antimicrobial Activity of Fungal Extract of The Aspergillus flavus from Hiri Island, North Maluku to Pathogenic Bacteria Sri Sedjati; Ambariyanto Ambariyanto; Agus Trianto; Endang Supriyantini; Ali Ridlo; Muhammad S. Bahry; Rizky Rifatma Jezzi; Mahadika Fanindhita Sany
Jurnal Kelautan Tropis Vol 23, No 1 (2020): JURNAL KELAUTAN TROPIS
Publisher : Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jkt.v23i1.7049

Antibacterial compounds from sponge association fungi are one of the alternatives to search for new antibiotics against resistant bacteria. This study aims to explore the secondary metabolites potential from sponge association fungi as MDR pathogens antibacterial and to cultivate these fungi using a variety of cultivation media. TE-BO-09.1. Isolate can inhibit 3 bacteria, K. pneumoniae, B subtilis, and S. aureus, but is not able to inhibit E. coli and P. aeruginosa. Cultivation using standard media of Malt Extract Agar (MEA) and media modified from fish broth (M1, M2) and cassava infusion (M3, M4) produce secondary metabolites with varying quantities and antimicrobial activity. The inhibition zone of the produced extract with 500 μg/disc concentration ranged from 7.14 to 10.32 mm. The strongest potential was shown by ethyl acetate extract from isolates cultured with M2 (9 days cultivation), of which is able to produce 10.32 mm inhibition zones against S. aureus and methanol extracts from isolates cultured with M4 (6 days cultivation), and able to produce 10.05 mm inhibition zones against K. pneumoniae. In conclusion, the fungus Aspergillus flavus can be culture using fish broth added glucose and cassava infusion water added peptone media to potentially produce antibacterial compounds against MDR pathogens.

Studi Morfometri dan Tingkat Kematangan Telur Kepiting Bakau (Scylla sp.) di Kawasan Perairan Demak Edi Wibowo; Suryono Suryono; Raden Ario; Ali Ridlo; Dodik S. Wicaksono
Jurnal Kelautan Tropis Vol 20, No 2 (2017): JURNAL KELAUTAN TROPIS
Publisher : Universitas Diponegoro

Maturity of Mangrove Crab (Scylla sp)is a decisive factor in the life cycle of mangrove crab (Scylla sp). Human activities such as over-fishing of mangrove crab to meet market needs regardless the size is one of the causes of the reduced number of individuals in the wild. This study aims to determine the correlation between morphometric of maturity stage of mangrove crab (Scylla sp) in Kedungmutih Waters, Demak District. The study used exploratory descriptive method which site determination using purposive sampling methods. The research consisted of female mangrove crab sampling and field water quality measurement (DO, temperature, salinity, and pH), morphometric measurements and weighing body weight of female mangrove crab samples, and observation of maturity stage in the laboratory.Thematerial used in this study were female mud crab (Scylla sp) obtained from Kedungmutih Waters, Demak District. The results of this study showed that 94 female mangrove crabs crabs were found to have a carapace width ranging from 77.50 mm - 126.45 mm, for body weight ranging from 87 grams to 359.78 grams. While the fecundity stage were obtained from the stage of I - IV and on the size of 200 grams - 300 grams has an optimal fecundity stage on the stage II and III. There is correlation between morphometry and maturity stage of mangrove crab (Scyla sp) because when the weight of crabs increases, so does the addition of the number of eggs and also improvement of egg (gonads). Kematangan telur pada Kepiting Bakau betina (Scylla sp) adalah faktor yang menentukan pada siklus hidup Kepiting Bakau (Scylla sp). Kegiatan manusia seperti penangkapan kepiting bakau yang tinggi untuk memenuhi kebutuhan pasar tanpa melihat ukuran yang ditangkap merupakan salah satu penyebab berkurangnya jumlah individu yang berada di alam. Penelitian ini bertujuan untuk mengetahui hubungan morfometrik terhadap tingkat kematangan tekur Kepiting Bakau Betina (Scylla sp) di kawasan perairan Kedungmutih,Kabupaten Demak. Metode penelitian yang digunakan adalah metode deskriptf eksploratif dengan penentuan lokasi menggunakan purposive sampling methods. Penelitian ini terdiri dari sampling kepiting bakau betina dan pengukuran kulaitas perairan di lapangan (DO, suhu , salinitas , dan pH), pengukuran morfometri dan penimbangan berat tubuh sampel kepiting bakau betina, dan pengamatan tingkat kematangan telur di laboratorium. Materi yang digunakan dalam penelitian ini adalah kepiting bakau betina (Scylla sp) yang diperoleh dari perairan Kedungmutih, Kadbupaten Demak. Hasil dari penelitian ini menunjukkan dari 94 ekor kepiting bakau betina yang didapatkan memiliki lebar karapas berkisar 77,50 mm – 126,45 mm, untuk berat tubuhnya berkisar 87 gram – 359,78 gram. Sedangkan pada tingkat kematangan telur didapatkan dari tingkat I – IV dan pada ukuran 200 gram – 300 gram memiliki tingkat kemmatangan telur yang optimal pada tingkat kematangan telur II dan III. Morfometri dan tingkat kematangan telur pada kepiting bakau betina (Scyla sp) memiliki hubungan dikarenakan jika pada bobot kepiting mengalami pembesaran maka pada telur mengalami penambahan jumlah dan mengalami peningkatan telur(gonad).

Struktur Komunitas dan Anatomi Rumput Laut di Perairan Teluk Awur, Jepara dan Pantai Krakal, Yogyakarta Rini Pramesti; AB. Susanto; Wilis A Setyati; Ali Ridlo; Subagiyo Subagiyo; Yohanes Oktaviaris
Jurnal Kelautan Tropis Vol 19, No 2 (2016): JURNAL KELAUTAN TROPIS
Publisher : Universitas Diponegoro

Seaweed has ecological benefits as well as economic value. Waters condition of Awur Bay and Krakal Beach supported this plant’s growth. There’s not yet the latest information about the vegetation. Utilization is still limited on some specieses. Tourist and inhabitant’s activities who take this plant would give impact to this plant’s vegetation. Therefore, it’s necessary to have data collecting, monitoring, and controlling at both of location. This research was aim to inventarisasi of seaweed for morphologic and anatomic characteristics at both of location. The research of method is explorative descriptive. The results showed that the amount of seaweed which was found at Awur Bay based on the morphology characteristics consist of two divisions was Chlorophyta (3 species) and Phaeophyta (5 species). Beside that, the amount of seaweed which was found at Krakal Beach based on the morphology characteristics consist of three divisons was Chlorophyta (4 species), Phaeophyta (2 species) and Rhodophyta (11 species). Three types of cell (anatomy) i.e. epidermis, kortex and medulla. The results of seaweed which found at Krakal Beach are density, frequency, cover percentage, important value index, and ecology index was taller than Awur Bay.Rumput laut bermanfaat secara ekologis maupun ekonomis. Kondisi perairan Teluk Awur, Jepara dan Pantai Krakal, Yogyakarta mendukung tumbuhan ini dapat tumbuh. Pemanfaatannya masih terbatas pada jenis tertentu. Aktivitas wisatawan dan penduduk sekitar yang mengambil tumbuhan ini akan berpengaruh sehingga perlu dilakukan penelitian tentang struktur komunitas di kedua lokasi tersebut. Penelitian ini bertujuan untuk mengetahui struktur komunitas, inventarisasi jenis baik secara morfologi dan anatomi. Metode yang digunakan yaitu metode deskriptif eksploratif. Hasil penelitian menunjukkan jumlah jenis rumput laut yang ditemukan di Teluk Awur terdiri dari dua divisi yaitu Chlorophyta (3 jenis) dan Phaeophyta (5 jenis). Jumlah jenis yang ditemukan di Pantai Krakal terdiri dari tiga divisi yaitu Chlorophyta (4 jenis), Phaeophyta (2 jenis) dan Rhodophyta (11 jenis). Tiga jenis sel penyusun thallus yaitu sel epidermis, korteks dan medulla. Struktur komunitas yang ditemukan di Pantai Krakal meliputi kepadatan, frekuensi, persentase penutupan, indeks nilai penting, dan indeks ekologi lebih tinggi daripada di Teluk Awur.

Mikroplastik pada Kedalaman Sedimen yang Berbeda di Pantai Ayah Kebumen Jawa Tengah Ali Ridlo; Raden Ario; Arif Maa’ruf Al Ayyub; Endang Supriyantini; Sri Sedjati
Jurnal Kelautan Tropis Vol 23, No 3 (2020): JURNAL KELAUTAN TROPIS
Publisher : Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jkt.v23i3.7424

Microplastics are one of the most significants pollutan in the marine environment and accumulate in sediments. The purpose of this research is to know the abundance of microplastics at different sediment depth in Ayah Beach Kebumen Central Java. The sediment samples were collected from three depth, 0-5 cm, 6–10 cm and 11–15 cm. microplastics were separated from sediment through density fracxination using NaCl solutiaon and ZnCl2 solution respectively. This study demonstrated that microplastics were consistenly found in all depth. The type of microplastics found were fiber, fragment and film. Fiber was the most frequent type (> 70%) in all samples. At 0–5 cm depth were found 578 microplastisc particle, at 6–10 cm depth were 459 particles, and at 11–15 cm depth were 610 particles. Mikroplastik merupakan salah satu polutan penting di laut dan terakumulasi di sedimen. Penelitian ini bertujuan untuk mengetahui kandungan mikroplastik di sedimen Pantai Ayah kebumen Jawa Tengah. Penelitian ini bertujuan untuk mengetahui kelimpahan mikroplastik pada kedalaman sedimen 0-5 cm, 6–10 cm dan 11–15 cm. Mikroplastik dipisahkan dari sedimen dengan cara fraksinasi berdasarkan densitasnya berturut-turut menggunakan larutan NaCl dan larutan ZnCl2. Hasil penelitian menunjukkan mikroplastik ditemukan pada setiap kedalaman. Bentuk mikroplastik yang ditemukan adalah fragmen, fiber dan film. Bentuk fiber menyusun lebih dari 70 % mikroplastik dalam sedimen di ketiga tingkat kedalaman. Pada kedalaman 0–5 cm terdapat 578 partikel mikroplastik, pada kedalaman 6–10 cm ditemukan 459 partikel, dan pada kedalaman 11–15 cm terdapat 610 partikel.

Skrining Dan Seleksi Bakteri Simbion Spons Penghasil Enzim Ekstraseluler Sebagai Agen Bioremediasi Bahan Organik Dan Biokontrol Vibriosis Pada Budidaya Udang Wilis Arii Setyati; Ahmad Saddam Habibi; Subagiyo Subagiyo; Ali Ridlo; Nirwani Soenardjo; Rini Pramesti
Jurnal Kelautan Tropis Vol 19, No 1 (2016): JURNAL KELAUTAN TROPIS
Publisher : Universitas Diponegoro

The rapid cultivation of aquaculture with the application of intensive systems in recent years, has raised problems in the form of a decrease in the carrying capacity of ponds for the life of aquaculture organisms. The further impact caused is the occurrence of a series of diseases and damage to environmental conditions. Anticipatory measures through the application of bioremediation are solutions to prevent more serious damage. In the bioremediation process, enzymes play the role of catalysts that accelerate biochemical reactions in ponds of soil and water. Bacteria associated with sponges have various bioactive compounds that can inhibit the growth of pathogenic bacteria. The purpose of this study is isolation and screening of bacteria associated with sponges that have extracellular enzyme activity (proteolytic, amylolytic, cellulolytic, and lipolytic) and antibacterial activity, as well as knowing the interaction of antagonism properties among the best isolates, and phenotypic identification of bacterial species. This research was conducted in 4 stages: sample collection, isolation, selection, and identification. The results showed that the total sponge symbiotic bacteria obtained were 15 isolates with the potential of 15 proteolytic isolates, 12 amylolytic isolates, 12 lipolytic isolates, and 4 cellulolytic isolates. There were 10 isolates having antibacterial activity against Vibrio harveyi and 2 isolates had antibacterial activity against Vibrio alginolyticus. The best bacterial isolates SP.1.3, SP.5.1, and SP.5.3 have no antagonistic activity between the three. Phenotypic identification of 3 isolates alleged that isolates SP.1.3, SP.5.1, and SP.5.2 were identified as Bacillus sp., Acinetobacter sp., And Pseudomonas sp.Pesatnya kegiatan budidaya perikanan dengan penerapan sistem intensif dalam beberapa tahun terakhir, telah memunculkan permasalahan berupa penurunan daya dukung tambak bagi kehidupan organisme budidaya. Dampak lanjut yang ditimbulkan adalah terjadinya serangkaian penyakit dan kerusakan kondisi lingkungan. Langkah antisipatif melalui penerapan bioremediasi merupakan solusi untuk mencegah kerusakan yang lebih serius. Dalam proses bioremediasi, enzim memainkan peran katalis yang mempercepat reaksi biokimia di kolam tanah dan air. Bakteri yang berasosiasi dengan spons memiliki beragam senyawa bioaktif yang dapat menghambat pertumbuhan bakteri patogen. Tujuan dari penelitian ini adalah isolasi dan skrining bakteri yang berasosiasi dengan spons yang memiliki aktivitas enzim ekstraseluler (proteolitik, amilolitik, selulolitik, dan lipolitik) dan aktivitas antibakteri, serta mengetahui interaksi sifat antagonisme antar isolat terbaik, dan identifikasi spesies bakteri secara fenotipik. Penelitian ini dilakukan dalam 4 tahap: koleksi sampel, isolasi, seleksi, dan identifikasi. Hasil penelitian menunjukkan bahwa total bakteri simbion spons yang diperoleh 15 isolat dengan potensi 15 isolat proteolitik, 12 isolat amilolitik, 12 isolat lipolitik, dan 4 isolat selulolitik. Terdapat 10 isolat memiliki aktivitas antibakteri terhadap Vibrio harveyi dan 2 isolat memiliki aktivitas antibakteri terhadap Vibrio alginolyticus. Isolat bakteri terbaik SP.1.3, SP.5.1, dan SP.5.3 tidak memiliki aktivitas saling antagonis antar ketiganya. Identifikasi fenotipik dari 3 isolat diduga bahwa isolat SP.1.3, SP.5.1, dan SP.5.2 diidentifikasi sebagai Bacillus sp., Acinetobacter sp., dan Pseudomonas sp.

Kandungan Total Fenolat pada Ekstrak Rhizophora sp Dari Teluk Awur, Jepara Ali Ridlo; Endang Supriyantini; Sri Sedjati
Jurnal Kelautan Tropis Vol 22, No 1 (2019): JURNAL KELAUTAN TROPIS
Publisher : Universitas Diponegoro

The antioxidant activity and total phenolic content of 18 extracts from different plant parts (leaves and stem bark) of 3 mangroves R. mucronata, R. apiculata andR. stylosa from Teluk Awur, Jeparawere examined. The content of total phenolics in the extracts was calculated as gallic acid equivalent (GAE) and antioxidant activity was estimated as IC50 values using 1,1-diphenyl-2-picrylhydrazyl (DPPH). The samples were extracted with n-hexane, ethyl acetate, and methanol respectively. The result showed that ethyl acetate extract of stem bark of R. apiculata holded the highest antioxidant activity with IC50 47.52ppm. Rates of total phenolic content ranged from 5.15 ± 1.02 to 64.79 ± 2.75 mg GAE/g.There was a significant correlation between total phenolic content and antioxidant activity. The results indicated promising mangrove Rhizophora sp. for the utilization as significant source of natural antioxidant. Telah dilakukan uji kandungan total fenolat dan aktivitas antioksidan 18 ekstrak dari bagian tanaman (daun dan kulit batang) 3 spesies mangrove asal Teluk Awur Jepara, yaitu mangroves R. mucronata, R. apiculatadan R. stylosa. Kandungan total fenolat ditentukan sebagai ekuivalen asam galat (GAE) dan aktivitas antioksidan ditentukan berdasarInhibition Concentration 50 (IC50) dengan menggunakan radikal bebas DPPH (1,1-diphenyl-2-picrylhydrazyl). Sampel diekstraksi secara bertngkat menggunakan n-heksana, etil asetat dan metanol. Hasil penelitian menunjukkan ekstrak etil asetat kulit kayu mangrove R. apiculata memiliki aktivitas antioksidan terbaik dengan IC5047.52ppm. Nilai kandungan total fenolat ekstrak berkisar antara 5.15 ± 1.02 sampai 64.79 ± 2.75 mg GAE/g. Terdapat korelasi kuat antara kandungan total fenolat dengan aktivitas antioksidan. Hasil ini menunjukkan bahwa mangrove Rhizophora sp. dapat dijadikan sebagai sumber senyawa antioksidan alami.

Pengaruh Cahaya Terhadap Produksi Fukosantin Chaetoceros calcitrans (Paulsen) Takano 1968 (Bacillariophyceae: Chaetocerotaceae) Sri Sedjati; Endang Supriyantini; Ali Ridlo; Ervia Yudiati; Linggar Dirgantara Prasetyo
Jurnal Kelautan Tropis Vol 22, No 2 (2019): JURNAL KELAUTAN TROPIS
Publisher : Universitas Diponegoro

Fucoxanthin is a pigment of the carotenoid group that can be used in the food and human health. The demand for natural carotenoid in the global market is quite high for food nutrition, food colorant, medicine, and cosmetic. Chaetoceros calcitrans included sea diatom rich in carotenoid, mainly fucoxanthin. This research aim is to optimize C. calcitrans cultivation using different light intensity treatment to create high fucoxanthin quantity. Light intensity treatment consisted of 4 levels : 1.000, 1.500, 2.000, and 2.500 lx with 2 replications. The culture process was done in medium-scale (60 L) for 14 days, enriched with diatom’s fertilizer, vitamin B, silica at temperature 25-27oC, salinity 32-33‰, pH 8-8,5, and DO 7-9 mg/g. Biomass and pigments were harvested in stationer phase, two days after the peak of logarithmic growth. Pigments analysis used Spektrofotometry method, absorbance values were measured in wavelengths 445, 632, 649, 663, 665, and 696 nm. The results showed that light intensity treatment was not significant in biomass weight (p=0,06), but it is very significant to cells density (p=0,01) and fucoxanthin production (p=0,01). In conclusion, the light intensity of 2.500 lx was effective to obtain the highest fucoxanthin at the amount of 10,13 ±1,62 mg dw with productivity at 0,17±0,03 mg/g culture media. The increase of light intensity correlated positively with the increase of fucoxanthin production with regression equation y = 0,006x-4,938 (r = 0,96). The increase in light intensity reaching 2500 lx in Chaetoceros calcitrans culture is proven can accelerate fucoxanthin biosynthesis, therefore the method can be applied to increase its production. Fukosantin merupakan salah satu karotenoid yang bermanfaat dalam bidang pangan dan kesehatan manusia. Permintaan karotenoid alami di pasar global besar sekali untuk berbagai kegunaan dalam bidang nutrisi makanan, pewarna makanan, obat-obatan, dan kosmetik. Chaetoceros calcitrans termasuk diatom laut yang kaya karotenoid, terutama fukosantin. Penelitian ini bertujuan untuk mengoptimasi kultur C. calcitrans dengan menggunakan perlakuan intensitas cahaya berbeda agar memproduksi fukosantin yang tinggi. Perlakuan intensitas cahaya terdiri dari 4 taraf : 1.000, 1.500, 2.000, dan 2.500 lx dengan 2 kali ulangan. Kultur dilakukan dalam skala medium (60 L) selama 14 hari, diperkaya dengan pupuk diatom, vitamin B, silikat pada suhu 25-27oC, salinitas 32-33‰, pH 8-8,5, dan DO 7-9 mg/g. Pemanenan biomassa dilakukan saat fase stasioner, yaitu 2 hari setelah puncak logaritmik. Analisis pigmen menggunakan metode Spektrofotometri, nilai absorbansi diukur pada panjang gelombang 445, 632, 649, 663, 665, dan 696 nm. Hasil penelitian membuktikan bahwa perlakuan intensitas cahaya tidak berpengaruh nyata (p=0,06) terhadap berat biomassa, tetapi sangat berpengaruh nyata terhadap kepadatan sel (p=0,01) dan produksi fukosantin (p=0,01). Pemberian intensitas cahaya 2.500 lx menghasilkan produksi fukosantin tertinggi, yaitu sebesar 10,13 ±1,62 mg dw dengan produktivitas sebesar 0,17±0,03 mg/g media kultur. Kenaikan intensitas cahaya berkorelasi positif dengan kenaikan produksi fukosantin dengan persamaan regresi y = 0,006x - 4,938 (r = 0,96). Peningkatan intensitas cahaya sampai 2500 lx pada kultur Chaetoceros calcitrans terbukti dapat memacu biosintesis fukosantin, sehingga metode tersebut bisa diaplikasikan untuk menaiknya produksinya.

Antibacterial Potential Chloroform Extract of Photobacterium phosphoreum Bacteria Symbiotic in Light Organs of Squid Loligo duvauceli Iin Putriyani; Delianis Pringgenies; Ali Ridlo
Jurnal Moluska Indonesia Vol. 4 No. 2 (2020): Oktober 2020
Publisher : Masyarakat Moluska Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.54115/jmi.v4i2.20

Microorganisms that live in association with marine invertebrates are able to produce a compound similar to those produced by marine invertebrates and are thought to be potential as bioactive materials. Supplement extracts from the Photobacterium phosphoreum bacterial culture have been shown to have antibacterial activity. The purpose of this study was to determine the anti-bacterial activity of the chloroform extract of Photobacterium phosphoreum biomass against pathogenic bacteria Bacillus substilis, Staphylococcus aureus, Salmonella sp. and Escherichia coli. The extraction process was carried out by the liquid-liquid extraction method. Fractionation was performed using Open Column Chromatography (KKT). The antibacterial activity test was conducted by the agar diffusion method according to Kirby-Bauer. The results showed that for the antibacterial activity test of crude extract, the largest inhibition zone diameter occurred in Escherichia coli with a concentration of 50 µg / disk at 24 h incubation, namely (9.80 ± 0.75 mm), while the smallest inhibition zone diameter occurred in Salmonella sp. by giving a concentration of 10 µg / disk at an incubation time of 48 hours, namely (6.03 ± 0.05) mm. Of the 6 fractions resulting from crude extract fractionation, it was known that fraction 5 was the most active fraction and inhibited the growth of the tested bacteria. The largest inhibition zone diameter occurred in Escherichia coli (9.83 ± 0.28) mm, while the smallest inhibition zone diameter occurred in Salmonella sp. (8.63 ± 0.20 mm)

Analysis of Fe and Zn content in green mussell Perna viridis at Bandengan beach Jepara and Tanjung Mas beach Semarang city Anita Dwi Kartika; Delianis Pringgenies; Ali Ridlo
Jurnal Moluska Indonesia Vol. 3 No. 1 (2019): April 2019
Publisher : Masyarakat Moluska Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.54115/jmi.v3i1.32

Green Mussel included to permanent animal so the interaction with its environment will affect its needed or its un-needed nutrient. Iron and zinc represent a very required micro mineral of human body. Iron plays an important role in forming red corpuscles and preventing anemia. The function and role of zinc in body for example to assist the forming of cell genetic items, forming the red corpuscle (haemoglobin), and also to assist pancreas function in digestion process. Territorial water of Jepara and Semarang are the representation areas producing Green Mussel (Perna Viridis). The aim of this research is to understand the iron and zinc content in Green Mussel. This research was conducted in December 2006 until February 2007. Materials used in this research is Green Mussel derived from Territorial water of Bandengan, Jepara and Territorial Water of Tanjung Mas, Semarang. The intake of Green Mussel sample used Purpose Random Sampling Method, which is intake of sample at random according to specific purpose. Method used in this research is Descriptive Method, while for iron and zinc measurement method using AAS. The result showed that the iron rate in Green Mussel at Territorial Water of Bandengan, Jepara degraded 459,70, 209,23 and 147,87 mg/kg from December 2006 until February 2007. While the Iron content in Green Mussel at Territorial Water of Tanjung Mas, Semarang continuously increased 227,18, 317,66 and 336,52 mg/kg from December 2006 until February 2007. The analysis result of zinc content on December until February of Jepara degraded 60,15, 8,90 and 7,73 mg/kg. While the Green Mussel Zinc of Tanjung Mas, Semarang on December 2006 until January 20.07 was degrading, from 154,10 become 9,63 and on January until February was increasing, from 9,63 mg/kg become 10,11 mg/kg.

Co-Authors A. Budi Susanto A.B. Susanto A.B. Susanto Adi Santosa Agus Sabdono Agus Trianto Ahmad Saddam Habibi Akbar, Muhamad Rahadian Alfin Anggraeni Ali Djunaedi Ambariyanto , Ambariyanto Ambariyanto Amtoni Caesario Nainggolan Anggi Setiabudi Anisah Harahap Anita Dwi Kartika Annisa Afifah Nugroho Anthony D Arnanda Antonius Budi Susanto Arif Maa’ruf Al Ayyub Arifin, Muhammad Sholeh Arvianto Wibowo Azizah, Pramita Bahry, Muhammad Syaifudien Bambang Yulianto Bara Yanwar Hadi Nugroho, Bara Yanwar Beladini, Septi Chrisna Adhi Suryono Christtenson Purba Christtenson Purba Cornelia Widya Seprandita Dafit Ariyanto Delianis Pringgenies Dewi, Lutfianna Fatma Dinda Ayuniar Zanjabila Dion Ragil Saputra Dion Ragil Saputra Dodik S. Wicaksono Dwi Haryo Ismunarti Edi Wibowo Eka Mulya Eko Sasmaya Eko Sasmaya Endang Supriyantini Endang Supriyantini Erma Nurmalitasari Ervia Yudiati Faishal Islami Faishal Islami, Faishal Faiz Naida Salimah Ghofari, Miftahul Akhyar Gunawan Widi Santosa Gunawan Widi Santosa Hafida Salma Hakim, M Sabron Sukmanul Harahap, Akbar Ibnu Pratikto Ibnu Wardani Iin Putriyani Irwani Irwani Ita Widowati Ivend Umbu Jawa Ivend Umbu Jawa, Ivend Umbu Jelita Rahma Hidayati Jusup Suprijanto Jusup Suprijanto Jusup Suprijanto Juwita Lesly Senduk Khusnul Khotimah Kirana Fatika Brilianti Koesoemadji Koesoemadji Laksono, Ollivia Brylliant Langit, Novita Thea Puspita Lilik Maslukah Linggar Dirgantara Prasetyo Lutfianna Fatma Dewi Mahadika Fanindhita Sany Manggola, Alen Mardani Mardani Masri, Mohammed Sharin Haji McCauley, Erin Miftahul Akhyar Ghofari Mohamad Mirza Mohamad Mirza Muhamad Rahadian Akbar Muhamad Rahadian Akbar Muhammad Abdul Zaky Muhammad S. Bahry Muhammad Zainuddin Nada Kristiani Ginting Nadya Oktavia Nerva Sembiring Nirwani Soenardjo Novita Thea Puspita Langit Nur Arif Kurniawan Nur Indah Febriani Nur Indah Febriani Ocky Karna Radjasa Oetari Kusuma Putri Ollivia Brylliant Laksono Pramastuti, Fransisca Ria Pramita Azizah Pratiwi, Siswantar Putri, Dhiya Aflah Luswanto Rachman, Rhima Rismiyati Raden Ario Raditya Ahmad Rifandi Radityo Haris Ratna Ibrahim Refi Sekarwardhani Retno Hartati Revo Raprika Kurniawan Ribka Anindita Br Perangin-angin Rifa’i, Akhmad Rini Pramesti RINI PRAMESTI Rizky Rifatma Jezzi Rizqi Umi Arifah Roestiawan, Dwi Bagus Salma, Hafida Salsabila, Nada Santoso, Gunawan Widi Savitri, Nadiah Humairoh Mufidah Sedjati, Sri Sedjati Septi Beladini Setia Devi Kurniasih Setia Devi Kurniasih, Setia Devi Setiyorini, Alin Sri Sedjati Sri Sedjati Sri Yulina Wulandari Subagiyo Subagiyo Subagiyo Subagiyo Subagiyo Subagiyo Sulistiana, Zalsabila Sunaryo Sunaryo Sunaryo Sunaryo Suryono Suryono Tri Aji Pamungkas Utomo, Wahyu Laurentius Pria Victorina Yulina Santi Wardani, Ibnu Widianingsih Widianingsih Wilis A Setyati Wilis A Setyati Wilis A Setyati Wilis Ari Setyati Wilis Ari Setyati Wismayanti, Gita Yohanes Oktaviaris Zaenal Arifin Zahari, Abdul Kadir Mulku Zaky, Muhammad Abdul

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