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The RELATION OF NEUTROPHIL ELASTASE LEVEL WITH TISSUE DESTRUCTION IN GINGIVITIS AND PERIODONTITIS: HUBUNGAN KADAR NEUTROFIL ELASTASE DENGAN KERUSAKAN JARINGAN PERIODONTAL PADA GINGIVITIS DAN PERIODONTITIS Kasuma, Nila
Dentika: Dental Journal Vol. 20 No. 2 (2017): Dentika Dental Journal
Publisher : TALENTA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (188.581 KB) | DOI: 10.32734/dentika.v20i2.160

Abstract

Periodontal disease consists of gingivitis and periodontitis. They occur due to the interaction between bacterial antigens and the host tissue hence neutrophil is activated, antibody is produced and bone resorption is occurred. Enzymatic changes to diagnose periodontal disease index (PDI) based on biomarker measurement scale is useful for an accurate diagnosis and a successful measurement of periodontal disease therapy. Gingival crevicular fluid (GCF) was selected as the biological medium. This research aims to analyze the relationship between neutrophil elastase level and periodontal tissue damages at gingivitis and periodontitis. The study involved 66 people as samples with 22 healthy samples, 22 mild gingivitis samples and 22 early periodontitis samples. Enzyme level studied was tested using ELISA technique. In this cross-sectional study, neutrophil elastase enzyme level was compared between healthy, mild gingivitis and early periodontitis sample groups. Data analysis was performed using ANOVA test. The average NE level based on PDI group is ± SD 2.65 ng/dl ± 1.28 in healthy sample group. There is an increase by 2.3 times in mild gingivitis sample group where NE level is ± SD 6.27 ng/dl ± 1.13.While, the light periodontitis group has NE level of ± SD 9.38 ng/dl ± 1.06 which equals to 3.5 times increase from the healthy sample group. This research concludes that the rise of neutrophil elastase level in gingival crevicular fluid is related to the severity of gingivitis and periodontitis diseases.
MORINDA CITRIFOLIA EXTRACT MOUTHWASH AS ANTIGINGIVITIS: OBAT KUMUR EKSTRAK BUAH MENGKUDU (MORINDA CITRIFOLIA L.) SEBAGAI ANTIGINGIVITIS Kasuma, Nila; Fajrin, Fildzah Nurul; Aldi, Yufri
Dentika: Dental Journal Vol. 19 No. 2 (2016): Dentika Dental Journal
Publisher : TALENTA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (371.689 KB) | DOI: 10.32734/dentika.v19i2.409

Abstract

Gingivitis is a inflamatory mild form of periodontal disease in gingiva. The early stage of gingivitis characterized by the accumulation of plaque, leukocytes, and PMN. Noni (Morinda citrifolia L.) is known to have anti-inflammatory compositions which also affects the activity of leukocytes during inflammation. The research objective was to analyze the clinical effects of mouthwash that contains extracts of noni (Morinda citrifolia L.) on leukocyte levels in the saliva in patients with gingivitis, in order to obtain traditional mouthwash that is economical, readily available, and effective in reducing gingivitis and can be used by the public. Extracts of noni fruit (Morinda citrifolia L.) is given in the form of mouthwashes used for 7 days in a row. Observations conducted on three groups of respondents: group 1 using mouthwash placebo, group 2 using mouthwash noni fruit extract 5%, and group 3 using chlorhexidine 0.1%. The use of mouthwash done in the morning and at night before bed as much as 15 ml for 30 seconds. The results showed a decrease in the number of leukocytes in the group treated with placebo cyst, 5% noni fruit extract, and chlorhexidine 0.1% (p <0.05). Both test preparations equally decreased the number of leukocytes in gingival sulcus in gingivitis patients.
SEXUAL DIMORPHISM OF MINANGKABAUNES’ MAXILLARY CENTRAL INCISOR’S WIDTH: DISMORFISME SEKSUAL DARI LEBAR INSISIVUS SENTRAL MAKSILA PADA SUKU MINANGKABAU Kasuma, Nila; Susi, Susi; Fitri, Haria; Fajrin, Fildzah Nurul
Dentika: Dental Journal Vol. 20 No. 1 (2017): Dentika Dental Journal
Publisher : TALENTA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (466.488 KB) | DOI: 10.32734/dentika.v20i1.705

Abstract

Teeth are known as organs made up of the hardest mineral tissue that is resistant to decay and physical, thermal, mechanical, chemical or biological damage. Tooth crown dimensions are reasonably accurate predictors of genders especially to young individuals whose secondary skeletal characteristics are yet developed. This research aims to analyse the influence of genders to the width of maxillary central incisor Minangkabaunese. The study was conducted using cross-sectional method in Luhak Agam, Luhak Tanah Datar and Luhak Lima Puluh Kota, West Sumatra using simple random sampling technique to a total of 120 samples. Teeth width was measured in mesiodistal dimension with 0.01 mm calibrated sliding digital caliper. The measurement was done on a tooth mould at ⅓ incisal as the position of mesial and biggest distar. T-test independent statistic test was done to determine the relationship between the 2 variables. Sexual dimorphism was determined with Garn formula. The research shows significant difference (p=0.001) between the width of left and right central incisor in males and females. The average width of the male and female right maxillary incisor is 8.545 mm and 8.370 mm, while the left central incisor width is 8.877 mm in male and 8.283 mm in females. Sexual dimorphism on the right central incisor is 2.09% and 3.43% on the left side. The conclusion of the study is that maxillary central incisor width can distinguish different genders in the identification process.
HUBUNGAN KONSUMSI SENG SEBAGAI KOFAKTOR MAKANAN TRADISIONAL MINANGKABAU DENGAN NEUTROFIL ELASTASE DALAM CAIRAN SULKUS GINGIVA PADA PENYAKIT PERIODONTAL: RELATION OF ZINC CONSUMPTION AS COFACTOR IN MINANGKABAU TRADITIONAL FOOD WITH NEUTROPHIL ELASTASE LEVEL IN GINGIVAL CREVICULAR FLUID IN PERIODONTAL DISEASE Nila Kasuma
Dentika: Dental Journal Vol. 18 No. 2 (2014): Dentika Dental Journal
Publisher : TALENTA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (172.628 KB) | DOI: 10.32734/dentika.v18i2.2010

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Penelitian ini melibatkan 66 orang sampel terdiri atas 22 orang sehat, 22 orang mengalami gingivitis ringan dan 22 orangmengalami periodontitis awal. Kadar enzim yang diteliti diuji dengan menggunakan teknik ELISA. Rancangan penelitianadalah cross sectional yang membandingkan kadar enzim netrofil elastase pada sampel sehat, gingivitis ringan danperiodontitis awal. Untuk melihat distribusi normal (p> 0,05) dilakukan tes Kolmogorov Smirnof. Terdapat kadarneutrofil elastase yang paling tinggi pada periodontitis ringan dengan rata-rata 9,42 ± 1,06 ng/dl. Kadar konsumsi sengyang paling tinggi adalah pada pasien sehat dengan rata-rata 6,39 ± 1,26 mg. Tes Pearson Correlation digunakan untukmembuktikan hubungan antara konsentrasi neutrofil elastase dengan konsumsi seng pada makanan tradisionalMinangkabau. Hasil penelitian menunjukkan korelasi yang signifikan ( p= 0,000) antara konsentrasi neutrofil elastasedengan kadar konsumsi seng pada makanan tradisional Minangkabau. Hubungan antara neutrofil elastase dan sengmenunjukkan korelasi yang kuat berarah negatif (r= -0,784). Sebagai kesimpulan, terdapat hubungan konsumsi sengsebagai kofaktor makanan tradisional Minangkabau dengan neutrofil elastase dalam gingival crevicular fluid penyakitperiodontal.
Jumlah Koloni Bakteri Asam Laktat pada Rongga Mulut yang Sehat Rheta Elkhaira; Nila Kasuma; Andani Eka Putra
Jurnal Kesehatan Andalas Vol 8, No 4 (2019): Online December 2019
Publisher : Fakultas Kedokteran, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jka.v8i4.1134

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AbstrakSalah satu flora normal yang terdapat di dalam rongga mulut adalah Bakteri Asam Laktat (BAL). Kemampuan BAL antara lain menghasilkan antimikroba, mengatur respons imun host dan menghalangi pertumbuhan bakteri patogen. Keberadaan BAL di rongga mulut sangat penting untuk menjaga kesehatan oral dan mencegah terjadinya penyakit periodontal. Hal ini mendorong perlunya penelitian mengenai jumlah koloni BAL di dalam rongga mulut yang sehat sehingga dapat dijadikan sebagai alternatif terapi penyakit periodontal. Tujuan: Mengidentifikasi jumlah koloni BAL pada rongga mulut yang sehat. Metode: Penelitian ini merupakan studi observasional dengan rancangan cross- sectional. Subyek penelitian terdiri dari 49 orang dengan gusi yang sehat. Subyek diminta untuk berkumur dengan akuades steril kemudian akuades hasil berkumur ditampung untuk selanjutnya dikultur di media MRS. Setelah diinkubasi selama 48 jam dilakukan penghitungan jumlah koloni bakteri asam laktat yang tumbuh pada media MRS. Hasil: Rerata jumlah koloni BAL kelompok sehat 7,8 x 104 ± 9,4 x 104 CFU/ml. Bakteri yang tumbuh pada media MRS pada penelitian ini semuanya berbentuk bundar, umumnya tepian rata, berwarna putih, mukoid, berukuran kecil dengan elevasi cembung. Beberapa bakteri berwarna krem atau putih keruh, berukuran sedang, tidak mukoid, tepian tidak rata dan elevasi seperti tombol. Simpulan: BAL merupakan flora normal yang keberadaannya penting untuk menjaga rongga mulut yang sehat. 
Pengaruh Aplikasi Bahan Pemutih Gigi Karbamid Peroksida 10% dan Hidrogen Peroksida 6% secara Home Bleaching terhadap Kekerasan Permukaan Email Gigi Meiyestri Dwi Riani; Fadil Oenzil; Nila Kasuma
Jurnal Kesehatan Andalas Vol 4, No 2 (2015)
Publisher : Fakultas Kedokteran, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jka.v4i2.252

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AbstrakHome bleaching adalah teknik pemutihan gigi vital yang dilakukan oleh pasien di rumah dalam pengawasan dokter gigi dengan konsentrasi karbamid peroksida 10-16% atau 3-6% hidrogen peroksida. Tujuan penelitian ini adalah untuk mengetahui pengaruh aplikasi bahan pemutih gigi karbamid peroksida 10% dan hidrogen peroksida 6% secara home bleaching terhadap kekerasan permukaan email gigi. Metode penelitian adalah true experimental dengan pretest-posttest control group design. Sampel berupa gigi premolar satu rahang atas yang sudah diekstraksi sebanyak 32 buah yang dibagi menjadi dua kelompok. Kelompok 1 adalah kelompok aplikasi bahan pemutih gigi karbamid peroksida 10% dengan lama aplikasi 1 jam perhari selama 14 hari dan kelompok II adalah kelompok aplikasi bahan pemutih gigi hidrogen peroksida 6% dengan lama aplikasi 10 menit perhari selama 14 hari. Selama aplikasi kedua sampel berada dalam inkubator 370C. Kekerasan permukaan email gigi diukur dengan alat Vickers Harndness Tester. Analisis data yang digunakan adalah uji t independen dan uji t dependen (p<0,05). Hasil uji statistik menunjukkan terjadinya penurunan yang sangat signifikan 0,000 (p<0,05) pada rerata nilai kekerasan permukaan email gigi antara sebelum dan setelah aplikasi bahan pemutih gigi. Kedua bahan pemutih gigi ini mempunyai perbandingan yang cenderung tidak signifikan 0,073 (p>0,05) terhadap kekerasan permukaan email gigi setelah aplikasi bahan pemutih gigi. Disimpulkan bahwa bahan pemutih gigi dapat menyebabkan penurunan kekerasan permukaan email gigi yang sangat signifikan karena terjadinya demineralisasi.Kata kunci: karbamid peroksida, hydrogen peroksida, home bleaching, kekerasan permukaan email gigiAbstractHome bleaching is whitening treatment of vital teeth conducted by a patient at home with under control of a dentist. The consentration of bleaching agents are 10-16% of carbamid peroxide or 3-6% of hidrogen peroxide. The objective of this study was to investigate the effect of application bleaching agents 10% of carbamide peroxide and 6% of hidrogen peroxide in home bleaching on enamel hardness surface.The method that used in this study is using true experimental with pre test-post test contol group design. Sample of the study using first premolar maxillary had been extracted into 32 pieces that divided into two treatments groups. Group I was applied by 10% of carbamide peroxide for 1 hours a day and group II was applied by 6% of hidrogen peroxide for 10 minutes a day and the duration of application was 14 days. Both of the groups were in the incubator 370C. Enamel hardness surface was measure by Vickers Hardness Tester. This study used independent t-test and paired sample t-test (p<0.05).The result of this study showed that significant decrasing 0.000 (p<0.05) of enamel hardness surface in average between before and after applied. Both of bleaching agents have the comparison that tend to be not significantly 0.073 (p>0.05) on enamel hardness surface after application of bleaching agents with significant value were. The conclusion is bleaching agents can affect enamel by decreasing enamel hardness surface value which are marked significant because demineralizion.Keywords:carbamide peroxide, hidrogen peroxide, home bleaching, enamel hardness surface
Hubungan Body Mass Index dengan Laju Aliran Saliva (Studi pada Mahasiswa Fakultas Kedokteran Gigi Universitas Andalas) Fildzah Nurul Fajrin; Zulkarnain Agus; Nila Kasuma
Majalah Kedokteran Gigi Indonesia Vol 1, No 2 (2015): December
Publisher : Faculty of Dentistry, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (255.15 KB) | DOI: 10.22146/majkedgiind.9230

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Relationship between Body Mass Index and Saliva Flow Rate (A Study in Faculty of Dentistry, Andalas University). Saliva flow rate is an affecting factor of caries formation. Adequate saliva flow rate can prevent cariess formation process and progression of periodontal disease and oral infection. Nutritional status is known as an influencing factor of saliva flow rate. The parameter of nutritional status is Body Mass Index (BMI). The purpose of this study is to discover the relation between BMI and saliva flow rate in College Students of the Dentistry Faculty Andalas University. This observational study with cross sectional design was conducted to 24 college students of the Dentistry Faculty Andalas University whose ages were 18-25 years old who complied with inclussion and exclussion criteria. BMI was calculated with BMI formula. The saliva flow rate was determined with Sialometry Method of Navazesh 2008 with ”ml/minute” unit. The univariat data analysis was caried out to describe each variable. Normality test Kolmogorov Smirnoff was done to see the normal distribution (p > 0,05). After the distribution was proven to be normal, corelation and regression test was executed to discover the relation between BMI and saliva flow rate. Based on the correlation test result, BMI of college students of the Dentistry Faculty Andalas University is χχ ± SD = 24,6 ± 6,02 kg/m2 and saliva flow rate is χχ ± SD = 0,29 ± 0,1 ml/minute. The relation between BMI and saliva flow rate expresses medium correlation with negative direction ( r = - 0,451). The result of linear regression shows that Saliva Flow Rate = 0,404 - 0,008*(BMI). The coefficient of regression BMI 0.008 shows that increasing 1 unit of BMI will decrease saliva flow rate 0,008 ml/minute. This study concludes that there is correlation between Body Mass Index and saliva flow rate. Obesity group has the lowest saliva flow rate, while the saliva flow rate does not decrease in underweight group.
Correlation between magnesium and alkaline phosphatase from gingival crevicular fluid periodontal diseases Nila Kasuma
Dental Journal (Majalah Kedokteran Gigi) Vol. 48 No. 3 (2015): September 2015
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (370.533 KB) | DOI: 10.20473/j.djmkg.v48.i3.p130-134

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Background: Magnesium is one of the alkaline phosphatase (ALP) cofactor. The amount of magnesium contained infoods affect ALP activity. Increased ALP activity will indicate the level of inflammation in periodontal disease. Elevated inflammation in periodontal disease will change gingivitis to periodontitis, where there has been damage to the bone ssupporting the teeth, and an increasing number of gingival crevicular fluid (GCF). The content of GCF consists of enzymatic and non-enzymatic. Changes in the composition of GCF occurs when the inflammation gets worse. Purpose: This study was aimed to prove the correlation between magnesium and ALP from GCF on periodontal disease. Method: This research involved 60 Minangkabaunese people with 20 healthy samples, 20 mild gingivitis samples, and 20 mild periodontitis samples. GCF was collected by absorbing method. Then ALP level in GCF was measured by using ELISA technique. Magnesium level in Minangkabaunese food was tested by Food Frequency Questionnaire (FFQ).Univariate analysis was performed to describe each variable. To see a normal distribution, Kolmogorov Smirnof Test was used (p>0.05). Unpaired T-test and Pearson correlation test was used to see correlation between ALP and magnesium level in Minangkabaunese food. Result: There is a significant correlation between the levels of ALP and magnesium level in Minangkabaunese food with periodontal disease (p=0.005). ALP is higest on mild periodontitis (137.74±23.01 ng/dl). Magnesium level normal control group is highest (250.14±32.34 mg) and in mild periodontitis is the lowest (110.83±21.04 mg). Corelation between ALP and magnesium level indicates strong correlation with negative direction (r=- 0.907). Conclusion: There is correlation between the levels of alkaline phosphatase and magnesium level on periodontal disease. Increasing inflamation rate will elevate the ALP level.
Effectiveness of Online Oral Health Education During the Covid-19 Pandemic Nila Kasuma1 , M. Biomed1 , Murniwati2 , Dedi Sumantri3 , Reni Nofika4 , Surya Nelis1 , Susi2, Aria
Indian Journal of Forensic Medicine & Toxicology Vol. 14 No. 4 (2020): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v14i4.12306

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This study aims at analyzing the effectiveness of online public education about oral health during theCOVID-19 pandemic. Studies show that humans touch their face 23 times in 1 hour and touch their mouthand its surroundings 4 times per hour with a duration of 1-12 seconds. This can cause the transmission ofthe virus through the oral, nasal, or conjunctival mucosa. How oral hygiene can contribute to reducing therisk of Corona Virus transmission between humans is the main focus of this study. Dental procedures carryhigh risk of exposure to Covid-19 through saliva, blood and other body fluids from patients, the use of highspeed instruments also increases the risk of infection in practice. The incubation period of 7-24 days exposesdentists and patients to cross-infection that can be transmitted through the oral cavity and respiratory tract.The study reveals that a great part of the population in Indonesia, especially in West is unaware of thetransmission of the Corona Virus through dental procedures. Consequently, the study suggests that onlineoral education is suitable for raising the community’s awareness of the Covid-19 pandemic.
BAKTI SOSIAL TELEDENTISTRY KEDOKTERAN GIGI, SUATU ALTERNATIF BAGI MASYARAKAT PADA MASA PANDEMI COVID-19 Haria Fitri; Suci Rahma Sari; Nila Kasuma; Rahmi Khairani; Dedi Sumantri
BULETIN ILMIAH NAGARI MEMBANGUN Vol 4 No 1 (2021)
Publisher : LPPM (Institute for Research and Community Services) Universitas Andalas Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/bina.v4i1.282

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Teledentistry is an examination and consultation of patients to the dentist without going through face-to-face visits, but with the help of an online application. The purpose of the activity is to help and facilitate Co-ass students of the Faculty of Dentistry, Andalas University, to fulfill the requirements for oral diagnosis via online (teledentistry) during the covid 19 pandemic. The activity is carried out in the form of examination/screening of patients carried out by Co-ass students whom Hospital Precept accompanies at the Faculty of Dentistry, Andalas University (Lecturer). Activities to be carried out consist of counseling, coordinator, mentoring, and monitoring of implementation and evaluation. The results of this activity can meet the patient's need for dental and oral examinations and expand access to dental care. Dental and oral health is essential for every individual, especially for carrying out functions such as eating, speaking, aesthetics, and feeling comfortable throughout the day. The Covid-19 pandemic limits the community's social conditions, including their need to get dental and oral services from dentists. In addition, dentistry is one of the jobs most at risk of contracting Covid-19 from patients. Through teledentistry activities, a dentist takes anamnesis to patients, clinical examinations are carried out through consultation through pictures and videos, diagnosis as initial information in planning further treatment, and supportive care such as patient education.