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Indra Kusuma
Department of Physiology, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia
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Journal : Current Biomedicine

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Effect of platelet-rich plasma on fibroblasts induced by lipopolysaccharide: in vitro study for wound healing Idris, Muthoharoh; Kusuma, Indra; Juniarti, Juniarti
Current Biomedicine Vol. 2 No. 1 (2024): January
Publisher : School of Veterinary Medicine and Biomedical Sciences, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/currbiomed.2.1.36-44

Abstract

Platelet-rich plasma (PRP) is expected to be an alternative therapy in wound healing by accelerating the inflammatory process and increasing the healing factors so that the healing process or inflammation is faster. The purpose of this study was to examine the effect of calcium chloride (CaCl2)-activated PRP on the viability, migration, and cytokine levels of interleukin-6 (IL-6) and vascular endothelial growth factor (VEGF) of dermal fibroblast cells in lipopolysaccharide (LPS)-induced inflammatory conditions, as a model in wound healing in vitro. Fibroblast cells were grown in DMEM medium induced with LPS, and then CaCl2-activated PRP treatment was added. Measure fibroblast cell viability using CCK-8 kit (cell counting kit-8) was evaluated using a microplate reader, and the cell migration was evaluated using scratch-assay and TScratch software. Expression of IL-6 and VEGF using ELISA kit. All data were analyzed using software SPSS version 26 by performing a one-way analysis of variance (ANOVA), Kruskal Wills, and Mann-Whitney tests. The results showed that PRP significantly increased fibroblast cell viability in the 10% PRP treatment group. This study shows that PRP does not reduce IL-6 cytokine levels but can increase VEGF growth factor in fibroblast cell cultures. PRP increased cell migration so that the healing process was faster. In conclusion, the CaCl2-activated PRP on LPS-induced fibroblast cells can increase viability and accelerate cell migration; it can’t decrease IL-6 but can increase VEGF expression. PRP is expected to be an alternative therapy in wound healing.
Co-Authors Agus Sulistiawan Ahmad Sulaeman Ajib Susanto Alida Harahap Alvita, Nini Aridestama, Raihan Aulia Arief Boediono Arief, Ilham Ascobat, Purwantyastuti Asnita, Rika Aufa, Akhdan Aulia, Maila Rizqi Avliya Quratul Marjan Azmi, Sabrina Azzahra, Sabna Bahri, Syukrini Bambang Kiranadi Bambang Purwoko Dewi, Hanik Anggraeni Djoko Sasono, Djoko Edy Supriyadi Eko Poerwanto Elsye Souvriyanti, Elsye Endang Purwaningsih Endy Muhammad Astiwara Erlina Burhan Erlina, Andi Erna Harfiani Evy Latifah Fachry Abda El Rahman Fadilah, Khonsa Fitri Fahrudin Fahrudin Fajrian, Muhammad Adhif Fasli Jalal Fauzi, Achmad Ma’ruf Fikriana, Chika Nur Fitria Athayya Desvianti Galih Saputra, Galih Gusnanto, Arief Hadi, Restu S. Hambani, Susy Hidayati, Anggi Puspa Nur Hidayati, Nur Ilham Risma Idris, Muthoharoh Ilmi, Ibnu Malkan Bakhrul Jemmy Rumengan Juniarti . Kesumaningtias, Raden Roro Widorini Lating, Noval Ali Lina Lasminingrum Muchtiar, Mulyadi Mulyadewi, Amelia Luthfiyah Mulyono, Ibnu Utomo Wahyu Mulyono, Ibnu Utomo Wahyu Noeriwan, Noeriwan Olivia, Prisca Pangestu, Hendri Pelangi Eka Yuwita Pratama, Muhammad Abel Priyono, Harim Putra, Andika Chandra Putri, Pingkan Permata Restu Syamsul Hadi Restuti, Ratna Dwi Rianda Dirkareshza Rianto Setiabudy Rifqatussaadah, Rifqatussaadah Rika Bur Rika Yuliwulandari Riliani, Marisa Ros Sumarny Samsul Mustofa, Muhammad Sapto Mei Budiyanto, Sapto Mei Sophie Yolanda Sulistiawati, Indah Syarifah, Lensy Khosinatus Taupiqqurrahman Tjandra Yoga Aditama Trijaya, Dewi Sekarsari Trinovita Andraini Utami Wahyuningsih Utomo, Ahmad Rusdan H Wening Sari Yati Sumiyati, Yati Yudertha, Andreo Yulia Suciati Yunazar Manjang Yurika Sandra Zannah, Fahma Hayyul