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Antioxidant Capacity, Phytochemical Profile, and Clustering of Pomegranate (Punica granatum L.) Peel Extracts Using Different Solvent Extraction Rafi, Mohamad; Wulansari, Laela; Septaningsih, Dewi Anggraini; Purnomo, Tsania Firqin; Auliatifani, Reza; Khaydanur, Khaydanur; Ilmiawati, Auliya; Yulianti, Wina; Nengsih, Nunuk Kurniati; Suparto, Irma Herawati; Kusuma, Wisnu Ananta
Journal of Tropical Life Science Vol 11, No 3 (2021)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Pomegranate has valuable nutritional content and contains various bioactive compounds, one found in the fruit's peel. The utilization of these bioactive compounds could be used as herbal medicines and supplements, such as antioxidants. This study aimed to determine the antioxidant capacity, phytochemical profile, and pomegranate peel extract grouping using different extracting solvents. The three extracting solvents used were water, 70% ethanol, and ethanol p.a. Antioxidant capacity of the three extracts was measured using the DPPH and CUPRAC methods. We also determined the total phenolic and flavonoid levels and the TLC fingerprint analysis and FTIR spectrum of the pomegranate peel extracts. The 70% ethanol extract owned the largest antioxidant capacity than the other two extracts with a value of 358.67 and 2981.59 µmol trolox/g dried sample using the DPPH and CUPRAC methods, respectively. The three pomegranate peel extracts' total phenolic and flavonoid levels ranged from 287.26–1068.81 mg GAE/g dried sample and 0.24-0.75 mg QE/g dried sample. TLC fingerprint analysis of pomegranate peel extract yielded 2, 6, and 6 bands for water extract, 70% ethanol, and p.a ethanol, respectively. The three extracts can be grouped based on FTIR spectrum data using principal component analysis using three principal components with a total variance of 93%. The results obtained show that using different extracting solvents provides different antioxidant capacities and phytochemical profiles.
Antioxidant Capacity, Phytochemical Profile, and Clustering of Pomegranate (Punica granatum L.) Peel Extracts Using Different Solvent Extraction Mohamad Rafi; Laela Wulansari; Dewi Anggraini Septaningsih; Tsania Firqin Purnomo; Reza Auliatifani; Khaydanur Khaydanur; Auliya Ilmiawati; Wina Yulianti; Nunuk Kurniati Nengsih; Irma Herawati Suparto; Wisnu Ananta Kusuma
Journal of Tropical Life Science Vol. 11 No. 3 (2021)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.11.03.14

Abstract

Pomegranate has valuable nutritional content and contains various bioactive compounds, one found in the fruit's peel. The utilization of these bioactive compounds could be used as herbal medicines and supplements, such as antioxidants. This study aimed to determine the antioxidant capacity, phytochemical profile, and pomegranate peel extract grouping using different extracting solvents. The three extracting solvents used were water, 70% ethanol, and ethanol p.a. Antioxidant capacity of the three extracts was measured using the DPPH and CUPRAC methods. We also determined the total phenolic and flavonoid levels and the TLC fingerprint analysis and FTIR spectrum of the pomegranate peel extracts. The 70% ethanol extract owned the largest antioxidant capacity than the other two extracts with a value of 358.67 and 2981.59 µmol trolox/g dried sample using the DPPH and CUPRAC methods, respectively. The three pomegranate peel extracts' total phenolic and flavonoid levels ranged from 287.26–1068.81 mg GAE/g dried sample and 0.24-0.75 mg QE/g dried sample. TLC fingerprint analysis of pomegranate peel extract yielded 2, 6, and 6 bands for water extract, 70% ethanol, and p.a ethanol, respectively. The three extracts can be grouped based on FTIR spectrum data using principal component analysis using three principal components with a total variance of 93%. The results obtained show that using different extracting solvents provides different antioxidant capacities and phytochemical profiles.
Co-Authors Abdul Aziz Abdul Rahman Saleh Adrianus, Albert Agus Buono Ahmad, Tarmizi Aini Fazriani Aisah Rini Susanti Alami, Tegar Ali Djamhuri Annisa Annisa Annisa , Annisa Annisa Annisa Annisa Annisa Annisa Annisa Annisa Annisa Anton Suryatama Arini Aha Pekuwali Arini Pekuwali Arwan Subakti Ary Prabowo Ary Prabowo Auliatifani, Reza Auliya Ilmiawati Auriza Rahmad Akbar Azizah, Norma Nur Azzahra, Syarifah Fathimah Badollahi Mustafa Badrut Tamam Bahrul Ulum BUDI TJAHJONO Budi Tjahjono Dahrul Syah Diah Handayani Dian Indah Savitri Dian Kartika Utami Essy Harnelly Fadli , Aulia Fahrury Romdendine, Muhammad Farhan Ramadhani , Hilmi Farit Mochamad Afendi Farohaji Kurniawan Fatriani, Rizka Fazriani, Aini Firman Ardiansyah Ginoga, Muh Fadhil Al-Haaq Halida Ernita Handayani, Vitri Aprilla Handayani, Vitri Aprilla Hanifah Nuryani Lioe Hardi, Wishnu Hasibuan, Lailan Sahrina Hendra Rahmawan Hendra Rahmawan Hera Dwi Novita Heru Sukoco Imas Sukaesih Sitanggang Indra Astuti Ira Maryati Irfan Wahyudin Irma Herawati Suparto Irman Hermadi Irmanida Batubara Irvan Lewenusa ISKANDAR ZULKARNAEN SIREGAR Isnan Mulia Janti G. Sudjana Jaya Sena Turana Joni Prasetyo Kana Saputra S Kangko, Danang Dwijo Karlisa Priandana Khaydanur Khaydanur Khaydanur, Khaydanur Laela Wulansari Larasati Larasati Lina Herlina Tresnawati Listina Setyarini Lusi Agus Setiani M. Rafi Maggy T. Suhartono Mala Nurilmala Medria Kusuma Dewi Hardhienata Mohamad Rafi Mohamad Rafi Mohamad Rafi Mohammad Romano Diansyah Mohammad Romano Diansyah Muchlishah Rosyadah Muhammad Asyhar Agmalaro Muhammad Subianto Mulyati Mulyati Mushthofa Muttaqin, Muhammad Rafi Nasution, Tegar Alami Nengsih, Nunuk Kurniati Norma Nur Azizah Nunuk Kurniati Nengsih Nur Choiriyati Nurdevi Noviana Ovi Sofia Pramita Andarwati Prihasuti Harsani Priyo Raharjo Pudji Muljono Purnajaya, Akhmad Rezki Purnomo, Tsania Firqin Ramadhanti, Nabila Sekar Ramdan Satra Ratu Mutiara Siregar Refianto Damai Darmawan Refianto Damai Darmawan Resnawati Reza Auliatifani Rif’ati, Lutfah Rizky Maulidya Afifa Ronald Marseno Rosy Aldina Rosyadah, Muchlishah Rudi Heryanto SATRIYAS ILYAS Septaningsih, Dewi Anggraini Siti Syahidatul Helma Sony Hartono Wijaya Sri Nurdiati SUHARINI, YUSTINA SRI Sulistyo Basuki Sulistyo Basuki Supriyanto, Arif Syahid Abdullah Syarifah Aini Syukriyansyah Taufik Djatna Toni Afandi Tsania Firqin Purnomo Usman, Muhammad Syafiuddin Wa Ode Rahma Agus Udaya Manarfa Wahjuni, Sri Widya Sari Wijaya, Eko Praja Hamid Wina Yulianti Wishnu Hardi Wulansari, Laela Yandra Arkeman Yessy Yanitasari Yudhi Trisna Atmajaya Yulianah Yulianah Yunita Fauzia Achmad Zulkarnaen, Silvia Alviani