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All Journal Jurnal Biologi Udayana ILMU KELAUTAN: Indonesian Journal of Marine Sciences Biotropika Current Biochemistry Journal of Innovation and Applied Technology Journal of Environmental Engineering and Sustainable Technology The Journal of Pure and Applied Chemistry Research Jurnal Kelautan Tropis Jurnal Sumberdaya Akuatik Indopasifik JFMR (Journal of Fisheries and Marine Research) Jurnal Biologi Tropis Indonesian Journal of Chemistry Jurnal Perikanan Universitas Gadjah Mada Jurnal Iktiologi Indonesia (Indonesian Journal of Ichthyology) Musamus Fisheries and Marine Journal Tomini Journal of Aquatic Science Bioscientist : Jurnal Ilmiah Biologi Nekton ACROPORA: Jurnal Ilmu Kelautan dan Perikanan Papua Jurnal Pijar MIPA STATISTIKA Jurnal Perikanan Berita Biologi Journal of Tropical Diversity
Muhammad Dailami, Muhammad
Aquaculture Study Program, Faculty Of Fisheries And Marine Science, Brawijaya University, Veteran Street, Malang 65145, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Engineering Environmental Science Immunology & microbiology Materials Science & Nanotechnology Mathematics Mechanical Engineering Medicine & Pharmacology Physics Social Sciences

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Journal : Current Biochemistry

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Phylogenetic Analysis of Cytochrome Oxidase I from Buduk Toads Duttaphrynus melanostictus and Phrynoidis asper from Bogor Muhammad Dailami; I Made Artika; Mirza Dikari Kusrini; Dodi Safari
Current Biochemistry Vol. 3 No. 2 (2016)
Publisher : IPB University

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Abstract

Indonesia have high diversity of Amphibians. Amphibians have an important role in ecosystem and produce many bioactive peptides. However, the genetic information of amphibians from Indonesia is very limited, especially Duttaphrynus melanostictus and Phrynoidis asper. The aims of this study are to determine the nucleotide sequence of cytochrome oxidase I (COI) from D. melanostictus and P. asper, to analyze their genetic diversity and their phylogenetic relationship. A total 668 base pairs of COI gene fragment were successfully amplified and their nucleotide sequence determined. P. asper (5 haplotypes) samples group have high haplotype diversity compared to D. melanostictus (1 haplotype). The results of Basic Local Alignment Search Tools (BLAST) to the NCBI and BOLD database, showed 99 % - 100 % identity to sequence of D. melanostictus. For the sequence of P. asper showed 99.23 % identity to sequence P. asper in BOLD database. There was no sequence of COI gene of P. asper in NCBI database. Genetic relationship among species in family Bufonidae, indicated that D. melanostictus has closer relation to P. asper than to another species, inspite of their pharapyletic characteristic. For intern species relationship of D. melanostictus, the data showed that D. melanostictus from Bogor have closer relationship to D. melanostictus from India than D. melanostictus from China.
Co-Authors Abdul Hamid A. Toha Abdul Hamid A. Toha Abdul Rahem Faqih Afnan, Naufal Izzudin Agoes Soeprijanto Agus Mohammad Hikam Aji Wahyu Anggoro Amalia, Elok Ambariyanto Ambariyanto Andrianus Sembiring Aradea Bujana Kusuma Ariyani, Destia Fitri Asus Maizar Suryanto H Ating Yuniarti Ating Yuniarti Aulia Rahmawati, Aulia Bayu Pranata, Bayu Buana Insan Kamil Christopher P. Meyer Condro Puspo Nugroho Dandi Saleky Dandi Saleky Dandi Saleky Dandi Saleky Dandi Saleky Dandi Saleky Darma Santi Dean Rama Prayoga Delvira Riska Ananta Denanta, I Made Kresna Dewa Gede Raka Wiadnya Dewa Gede Raka Wiadnya Diah Permata Wijayanti Dodi Safari Dyah Kinasih Wuragil EDY H.P. MELMAMBESSY Eka Maya Kurniasih Elsa Syafi’ul Umma Emmanuel Manangkalangi Febriyana Novita Damayanti Febriyani Eka Supriatin Febriyani Eka Supriyatin Firda, Wahyuma Fitri Nur Azizah Harahap, Muhammad Alfiandi Rachmad Hardoko Hardoko Hardoko Hardoko Hariati, Anik Martinah Hariyadi, Hariyadi Heder Djamaludin Hendrikus R Siga Hermawaty Abubakar I MADE ARTIKA Indria Graha Hayuningtyas Isma Isma Jeni, Jeni Kholil, Kiki Nur Azzam Kusuma, Wahyu Endra Lalu Panji Imam Agamawan Maftuch Maftuch Mahariawan, I Made Dedi Maheno Sri Widodo, Maheno Sri Manan, Jemmy Manumpil, Abraham Welliam Marius Agustinus Welliken Maulana, Antajala Rian Mayor, Corazon E.M.P. Mayor, Corazon Ell Merilia Papuani Milansari, Sindi Mirza Dikari Kusrini Mohammad Sholeh Uluwwi Mudjirahayu Mudjirahayu Muhammad Fakhri Muhammad Musa Murtihapsari . Nasrullah Bai Arifin Ni Kadek Dita Cahyani Ni Putu Dian Pertiwi Novaldo Yuri Muhammad Nugroho, Nurul Nurcahyani, Shinta Eka Nurul Jadid Mubarokati Paricahya, Akhsan Fikrillah Permatasari, Felyta Dian Pratama, Rakhmad Ndaru Putri Nur Azizah Putri, Ajeng Ayu Restuaning Putri, Radwa Fathin H Rahardjo, Seto Sugianto Prabowo Ramadhan, Surya Dewa Reny Sianturi Renyaan, Alberth W. Roni Bawole Rosa D. Pangaribuan RR. Ella Evrita Hestiandari Saleky, Dandi Salsabila, Sivana Yesica Ayu Satria, Ibnu Darma Siahaan, Marthin Daniel Theo Gratia Simon P.O Leatemia Sri Andayani Suciati, Lusia Dyah Sufaichusan, Ifa Supriatin, Febriyani Eka Surya Dewa Ramadhan Thomas Frans Pattiasina Tresia Tururaja Tristany Brighton Jorghi Vivi Nurhadianty Vivi Nurhadianty, Vivi Widyawati, Yuni Yenny Risjani Yuni Widyawati Yuni Widyawati Yusup A Jentewo Zeth Parinding