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Journal : Borneo Journal Of Pharmascientech

AKTIVITAS ANTIOKSIDAN DAN KADAR FENOLIK TOTAL DARI EKSTRAK DAUN KACIP FATIMAH (Labisia pumila) MENGGUNAKAN VARIASI PELARUT EKSTRAKSI Rahmatullah, Satrio Wibowo; Rizki, Muhammad Ikhwan; Sari, Anna Khumaira; Triyasmono, Liling; Normaidah, Normaidah; Lingga, Herningtyas Nautika
BORNEO JOURNAL OF PHARMASCIENTECH Vol 8 No 2 (2024): Borneo Journal of Pharmascientech
Publisher : Universitas Borneo Lestari

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51817/bjp.v8i2.538

Abstract

Secondary metabolite content and extract characterization can be influenced by the solvent used in the extraction process. The content of phenolic compounds and antioxidant activity of Labisia pumila leaves can be influenced by solvents. This research aims to determine three extracts' antioxidant activity and total phenolic content using different solvents. Fresh L. pumila leaf samples were cleaned with running water and then dried using a drying cabinet. The dried leaves are then powdered and extracted using distilled water, 70% ethanol, and 96% ethanol. Extraction with distilled water using the infusion method and ethanol using maceration. Next, the solvent obtained was evaporated using a rotary evaporator and oven until a dry extract was obtained. Total phenolic content was determined using the UV-Vis spectrophorometric method with the Folin-Ciocalteu reagent. The standard used is error-free. Antioxidant activity was determined using the DPPH method with a UV-Vis spectrophotometer, then the IC50 value was calculated. The research showed that the total phenolic content of the distilled water extract was 6.437 ± 0.177 w/w GAE, the 70% ethanol extract was 8.377 ± 0.079 w/w GAE, and the 96% ethanol extract was 17.387 ± 0.621% w/w GAE. Antioxidant activity is known to have an IC50 value of distilled water extract of 59,716 ppm, 70% ethanol extract of 50,543 ppm, and ethanol extract of 43,520 ppm. The conclusion of the research is that of the three extracts, it is known that the antioxidant activity is the strongest, and the total phenolics are the highest in the 96% ethanol extract.
Co-Authors Abda Abda Abdul Gafur Agung Endro Nugroho Agung Nugroho Agustina, Ni Kadek Ayu Akbar, Nabila Hadiah Alfandani, Gina Alifni Adha Bakti Amali, Dzikra Aulia Ana Ulfah Anna Khumaira Sari Anna Khumaira Sari Arnida Arnida Arnida Arnida Beny Rahmanto Beny Rahmanto Beny Rahmanto Budi Prayitno Buih, Putri Helena Junjung Destria Indah Sari Dewi, Anita Ratna Dewita Fitri Widodo Difa Intannia Dika Wardana, Dika Dina Rahmawanty Dodon Turianto Nugrahadi Effendi, Ibrahim Rully Eko Suhartono Evi Mintowati Evi Mintowati Kuntorini Evi Mintowati Kuntorini Evi Mintowati, Evi Fajar Lestari Fajar Lestari Fajar Lestari Gina Alfandani Gunawan Gunawan Gunawan Gunawan Hayatun Izma Helmina Wati Heri Budi Santoso Herningtyas Nautika Lingga Humaidy, Mizan Irvan Ipand Irvan Ipandi, Irvan Izma, Hayatun Khoerul Anwar Khoerul Anwar Khoerul Anwar Khoerul Anwar Khumaira Sari, Anna Laura Sarwo Malahayati, Siti Malikhatun Ni’mah, Malikhatun Malikhatun Ni’mah Maman Turjaman Maman Turjaman Mia Fitriana Muhammad Ikhwan Rizki Muhammad Janawi Muhammad Reza Faisal, Muhammad Reza Muslimah Muslimah Mutia Mawaddah Nazhipah Isnani Noor Rizkiyah Normaidah, Normaidah Nugroho, Agung Nur Ainah Nur Mahdi Nuraji, Tara Pramesti Nurlely Nurlely Nurlely, Nurlely Ori Minarto Rahmah, Indah Noor Rahmanto, Beny Rahmatullah, Satrio Wibowo Rahmi Safitri Rahmi Safitri, Rahmi Ratnapuri, Prima Happy Redanti, Hema Novita Rorry Adivrio Rizky S.Pd. M Kes I Ketut Sudiana . Salamiah . Salamiah Salamiah Salsabilla, Risma Zahra Salwa, Helmina Samsul Hadi Sandi, Dita Ayulia Dwi Sarlina Illyyani Sarlina Illyyani, Sarlina Selamat Alfiani, Selamat Sofia Sri Cahyo Wahyono Sudarsono Sudarsono Susiani, Eka Fitri Susy Andriani Sutomo Sutomo Sutomo Sutomo Totok Wianto Veithzal Rivai Zainal Wawan Halwany Wawan Halwany Wawan Halwany Wawan Halwany, Wawan