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All Journal Jurnal Fitopatologi Indonesia Jurnal Kimia (Journal of Chemistry) Agrikultura Biotika: Jurnal Ilmiah Biologi Journal of Marine Research Cropsaver : Journal of Plant Protection Padjadjaran Journal of Dentistry Chimica et Natura Acta Indonesian Journal of Chemistry Molekul: Jurnal Ilmiah Kimia Makara Journal of Science Molekul: Jurnal Ilmiah Kimia Media Penelitian dan Pengembangan Kesehatan
Tri Mayanti
Jurusan Kimia, Fakultas Matematika Dan Ilmu Pengetahuan Alam, Universitas Padjadjaran
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Decision Sciences, Operations Research & Management Dentistry Earth & Planetary Sciences Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Public Health

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Kemampuan Senyawa Volatil Aureobasidium pullulans pada Beberapa Kerapatan Sel dalam Menekan Antraknosa Stroberi Hartati, Sri; Nurjihan, Khansa; Susanto, Agus; Istifadah, Noor; Mayanti, Tri
Agrikultura Vol 36, No 2 (2025): Agustus, 2025
Publisher : Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/agrikultura.v36i2.62929

Abstract

Antraknosa yang disebabkan oleh Colletotrichum acutatum menjadi salah satu penyakit utama pada buah stroberi. Pemanfaatan senyawa volatil khamir dapat menjadi salah satu alternatif pengendalian penyakit pascapanen yang aman dan ramah lingkungan. Kerapatan sel khamir dapat berpengaruh terhadap keefektifan cara pengendalian tersebut. Penelitian ini bertujuan untuk menguji kemampuan senyawa volatil khamir Aureobasidium pullulans Dmg 11 DEP pada beberapa kerapatan sel dalam menghambat pertumbuhan C. acutatum dan menekan penyakit antraknosa pada buah stroberi, serta untuk mendapatkan kerapatan sel khamir yang mampu menghasilkan penekanan tertinggi terhadap penyakit antraknosa pada buah stroberi. Penelitian menggunakan Rancangan Acak Lengkap (RAL) yang dilakukan secara in vitro dan in vivo. Perlakuan terdiri atas kerapatan sel khamir A. pullulans Dmg 11 DEP yaitu 106 sel/ml, 107 sel/ml, 108 sel/ml, 109 sel/ml, dan kontrol. Pengujian in vitro dilakukan dengan metode double dishes system pada media PDA. Pengujian in vivo dilakukan pada buah stroberi varietas Mencir dengan menempatkan khamir tanpa kontak fisik dengan buah. Hasil penelitian menunjukkan bahwa senyawa volatil khamir A. pullulans Dmg 11 DEP pada beberapa kerapatan sel mampu menghambat pertumbuhan C. acutatum secara in vitro sebesar 34,98% - 42,64% dan mampu menekan penyakit antraknosa pada buah stroberi sebesar 18,27% - 36,46%. Kerapatan sel 108 sel/ml menunjukkan persentase penekanan penyakit antraknosa tertinggi pada buah stroberi.
Synthesis of Two Analogues of Xylapeptide A and Their Potency as New Antimicrobial Agent Maharani, Rani; Muchlis, Handi Nugraha; Hidayat, Ace Tatang; Al-Anshori, Jamaludin; Nurlelasari, Nurlelasari; Harneti, Desi; Mayanti, Tri; Farabi, Kindi; Supratman, Unang
Indonesian Journal of Chemistry Vol 25, No 5 (2025)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijc.100353

Abstract

Xylapeptide A, derived from the fungus Xylaria sp. x Sophora tonkinensis, exhibits potent and selective antimicrobial properties. Our research group has successfully synthesized xylapeptide A. In our recent work, two xylapeptide A analogues (An1 and An2) were synthesized using a combination of solid- and solution-phase synthesis methods. The linear precursors of An1 and An2 were synthesized on 2-CTC resin with the Fmoc strategy. The coupling reagents HBTU/HOBt and HATU/HOAt were employed. Subsequently, the linear precursor was cleaved from the resin using either 20% TFA or a TFE mixture, and then cyclized in solution phase with HBTU. The synthesized products were purified using semi-preparative RP-HPLC, giving the percent yields 16% for An1 and 12% for An2. Both compounds were then characterized by HR-ToF-MS, 1H- and 13C-NMR. The synthesized xylapeptide A and its analogues were evaluated against Bacillus cereus, Bacillus subtilis, Staphylococcus aureus, Enterococcus faecalis, Escherichia coli, Klebsiella pneumoniae, and Candida albicans. The result showed that An2, possessing arginine residue, exhibited higher activity compared to xylapeptide A and An1. This research suggests that xylapeptide A analogues hold great promise as novel antimicrobial agents.
Co-Authors Abdillah, Lutfi Ace Tatang Hidayat Agus Susanto Ajeng Diantini, Ajeng Andre A. Sonda Anindyta, Annisa Anjari, Intan Hawina Aoliya, Salwa Rohmatul Azmi, Mohamad Nurul Azzahra, Fathia Darwati Darwati Darwati, Deden Indra Dinata, Deden Indra Desi Harneti Putri Huspa Dewa Gede Katja Dikdik Kurnia DUDI RUNADI Elizabeth Fitriana Sari Endah Yulia Euis Julaeha Fajar Fauzi Abdullah Farabi, Kindi Ferry Ferdiansyah Sofian, Ferry Ferdiansyah Gunawan, Latifah Harizon Harizon Hideo Hayashi Hilmayanti, Erina Indriyani, Indri Isramiharti Isramiharti Jamaludin Al-Anshori Kautsari, Arsi Khalijah Awang Kindi Farabi Kindi Farabi Kindi Farabi Kusumiyati Madihah Madihah Maira, Faizah Mayshah Purnamasari Mohamad Nurzaman Muchlis, Handi Nugraha Muhammad Andry Muhammad Hanafi Mustaqim, Iqbal Wahyu Mustofa, Hidayat Nurul Nafiah, Mohamad Azlan Naini, Al Arofatus Noor Istifadah Nurcahyanti, Ois Nurjihan, Khansa Nurlelasari Nurlelasari Purnama Purnama Qomarilla, Nurul Rani Maharani Reina Yulianti Reina Yulianti Yulianti Rida Evalina Tarigan RIKA MELIANSYAH Romundza, Febbry Ronny Lesmana Salam, Supriatno Sandra Amalia Riyadi Setiani, Cahya Shiono, Yoshihito Sianturi, Julinton Siska Elisahbet Sinaga Sofa Fajriah Sri Hartati Sri Rejeki Rahayuningsih Surbakti, Chemayanti Tati Herlina Tenny Putri Wikayani, Tenny Putri Unang Supratman Wahyuni, Aneu Wawan Hermawan Wibisono, Aditya Seiza Yoshihito Shiono Yulisar, Riza Zahidah Nurulhaq