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Induksi Kalus dan Regenerasi Tanaman pada Kultur Antera Persilangan Padi Indica Varietas Lokal Bengkulu Herawati, Reny; Rustikawati, Rustikawati; Inoriyah, Entang
Akta Agrosia Vol 18 No 2 (2015)
Publisher : Badan Penerbitan Fakultas Pertanian (BPFP), Fakultas Pertanian, Universitas Bengkkulu

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Abstract

Establishment of homozygous lines can be accelerated with anther culture technique that can produce pure lines in one generation. Formation of spontaneous double haploid plants in rice anther culture is very beneficial, because it does not need to be doubled haploid plants as material selection.This study aims to obtain a doubled haploid lines of upland rice is tolerant to drought and blast disease resistance. The main ingredient in this research is anther crosses of rice plants (F1), which consists of local varieties with selected lines of P1 (Sriwijaya x IR-148), P2 (Sriwijaya x IR-7858-1), P3 (Bugis x IR -148), and P4 (Bugis x IR-7858-1), callus induction media (N6), regeneration medium (MS). Anther culture of local varieties of rice indica/indica produced callus induction and plant regeneration are low, resulting in low efficiency of anther culture in providing green plants. The use of donor parents indica/j aponica which is responsive to anther culture into consideration for the material F1 crosses. Anther inoculation needs to be done in several stages so that the opportunity to obtain plantlets in sufficient quantities for material selection will be greater.
Potensi Produksi Enam Hibrida Jagung pada Ultisol di Provinsi Bengkulu Suprijono, Eko; Rustikawati, Rustikawati; Romeida, Atra; Gustian, Meko
Akta Agrosia Vol 18 No 2 (2015)
Publisher : Badan Penerbitan Fakultas Pertanian (BPFP), Fakultas Pertanian, Universitas Bengkkulu

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Abstract

Ultisol is one of marginal land type quite extensively presence in Indonesia, including the province of Bengkulu. The use of varieties tolerance to acidity stress is the best manner to utilize of this type of land with relatively low cost and environmentally friendly. The objective of this research was to evaluate theyield potential of six maize hybrids developed to soil acidity tolerance. The experiment was conducted at the Experimental Farm Medan Baru, Kandang Limun Village, Muara Bangkahulu District, Bengkulu City, from August to November 2015. The experiment was arranged in a randomized completely block design with four replications. As the treatment were newly developed maize hybrids, namely CT5, CT8, CT9, CT13, CT14 and CT15. The variables measured were cob length, cob diameter, 100-seed weight and the estimated yield/ha. Qualitative variables supporting to corn yield were the cob position, seed color, seed type and seed row arrangement. The results indicated that the hybrid CT8 showed the highest estimated yield in Ultisol among hybrids evaluated, with the estimation of 6.5 tons per hectare. Qualitatively, theseeds of hybrid CT8 were a horse tooth type, pale yellow color, and stright line arrangement.
Performance of Fifteen F5 Pedigree Upland Rice Lines in Ultisol Luwih, Bagus Edi; Rustikawati, Rustikawati; Hindarto, Kanang Setyo
Akta Agrosia Vol 20 No 2 (2017)
Publisher : Badan Penerbitan Fakultas Pertanian (BPFP), Fakultas Pertanian, Universitas Bengkkulu

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Abstract

ABSTRACTRice (Oryza sativa L.) is a rice-producing crop that is the main food source for most of Indonesia's population. Although rice production increases but needs to be maintained. One of the efforts in maintaining rice production is by assembling superior paddy varieties that are adaptive to Ultisol soil. This study aims to compare the appearance of 15 lines of upland rice grown in Ultisol soil and choose the best lines based on index selection. The research was conducted on August 2015 until January 2016 at New Field Experiment Garden of Agriculture Faculty of Bengkulu University of Kandang Limun Sub-District, Muara Bangkahulu Sub-district, Bengkulu City. The experimental design was Randomized Block Design (RAKL) with 3 replications. As the treatment is 15 lines of rice are G1, G2, G3, G5, G8, G13, G16, G24, G30, G32, G35, G40, G44, G45, and G50. Of the 15 gogo rice strains tested showed that the generative and vegetative variables did not significantly affect the number of leaves, the number of productive tillers, the number of pithy grains per panicle, the weight of the grain per panicle, the weight of 100 grains, and the weight of grain per hill.keywords: upland rice, Ultisol, yield
Penapisan Tiga Puluh Tujuh Genotipe Tomat dan Seleksi Primer RAPD untuk Toleransi terhadap Layu Bakteri (Ralstonia solanacearum) Adeputri, Eriana; Rustikawati, Rustikawati; Herison, Catur
Akta Agrosia Vol 19 No 1 (2016)
Publisher : Badan Penerbitan Fakultas Pertanian (BPFP), Fakultas Pertanian, Universitas Bengkkulu

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Abstract

Bacterial wilt (Ralstonia solanacearum) is the most important tomato diseases which can reduce tomato yield up to 100%. One most prospective control measure is the development of high yielding varieties tolerance to bacterial wilt. Donor parent carrying bacterial wilt controlling gene(s) is required to develop such varieties. The objectives of this study were to screen thirty-seven tomato genotypes for tolerance to R. solanacearum and to obtain RAPD markers of resistance to bacterial wilt. The experiment was arranged without the experimental design. Each tomato genotypes consisting of 5 control uninoculated plants and 10 plants were inoculated with R. solanacearum with a concentration of 106 cfu / ml inoculum of 10 mL per plant. Scoring was done on the severity of disease symptom; and the plants were grouped according to scoring class. Molecular analysis was done by using Bulk Segregant Analysis (BSA). The results showed there were six genotypes considerd very tolerant i.e. genotype Pearl, Opal, Cung, Syu and Kudamati I; tolerant genotypes consisting of five genotypes; medium tolerant groups consisting of eight genotypes; medium sensitives groups consisting of 14 genotypes; and sensitive class consisting of four genotypes, namely Aceh Local Tomatoes I, Tanah Datar Local Tomato, Tomato Meranti I and Tomato Meranti II. Of the 20 primers used only 8 primers showed visible DNA bands, they were E1, E7, E10, H2, H5, H13, H16 and H19. However there no polymorphics bands observed for bacterial wilt tolerance.
Keragaan Pertumbuhan dan Hasil Enam Belas Genotipe Tomat (Solanum lycopersicum L.) di Dataran Rendah Pardosi, Santi K.; Ruistikawati, Rustikawati; Suryati, Dotti
Akta Agrosia Vol 19 No 2 (2016)
Publisher : Badan Penerbitan Fakultas Pertanian (BPFP), Fakultas Pertanian, Universitas Bengkkulu

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Abstract

Tomato is a high nutritional value fruit vegetables. Genetic variation is valuable plant breeding material which can be measured in terms of yield potential. This study aimed to compare the growth and yield components of 16 tomato genotypes in the lowlands. The experiment was conducted in Medan Baru Experimental Station of, Faculty of Agriculture, University of Bengkulu, with the elevation of 10 m above sea level. This was performed from September 2013 to January 2014. The experiment was set in a completely randomized design with a single factor of 16 tomato genotypes with 3 replications acounting to 48 experimental units, each of which consisted of 2 plants. The sixteen genotypes tested were Mirah, Berlian, CIN 06, SU, Kudamati 3, Lombok 3, Lombok 4, Makassar, Aceh 3, Aceh 5, Ranti Situbondo, Ranti, Situbondo Gelombang, Situbondo, Kemir, Meranti 2, Gondol Lonjong. There were significant differences in plant height, stem diameter, number of leaves, stomatal density, flowering age, harvest age, fruit diameter, fruit sweetness, fruit hardness, total fruit weight, fresh weight, and dry weight. Based on total fruit weight. The genotypes potentially cultivated in the lowlands are Ranti, Situbondo Gelombang, Kudamati 3, Situbondo, and Makassar.
Combining ability of six s7 generation of corn hybrid parents from half dialel crossesof Maize in a Half Diallel Crosses Dwitama, Niko; Rustikawati, Rustikawati; Suryati, Dotti; Herison, Catur
Akta Agrosia Vol 21 No 1 (2018)
Publisher : Badan Penerbitan Fakultas Pertanian (BPFP), Fakultas Pertanian, Universitas Bengkkulu

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Abstract

Combining ability is the genotype's ability to pass on the desired character to the offspring.  Combining ability information is needed to determine the crossed pairs in the formation of hybrid varieties.  The purpose of this study was to obtain information on the combining ability value of six S7 generation of inbreed maize.  The experiment was conducted in September 2015 until January 2016, at Medan Baru Experimental Station of Faculty of Agriculture, at Kandang Limun village, Muara Bangkahulu Sub-district, Bengkulu City.  The experiment was prepared in a Randomized Block Design (RCBD) with 15 corn hybrid treatments including: G1XG3, G1XG6, G3XG6, G1XG7, G3XG7, G6XG7, G1XG8, G3XG8, G6XG8, G7XG8, G1XG9, G3XG9, G6XG9, G7XG9 and G8xG9.  Each experimental unit was repeated three times obtaining 45 experimental plots.  Estimation of general combining ability (GCA) value  and specific combining ability (SCA) of each inbreed strain were conducted with Griffing model IV method.  The results showed that genotype G1 and G3 had positive GCA values for all characters except plant height.  The G7XG1 cross combination showed a positive SCA on all characters even higher in plant height and stem diameter. The G8XG6 cross combination showed high SCA values for leaf number characters, weight of husk-less cob, number of seeds per seed-line, weight of grain per ear, and grain yield per plot.Keywords: Hybrid, corn, GCA and SCA
Morphological Characterization of 10 Hot Pepper Genotipes in Low Altitude Location Herison, Catur; Surmaini, Ervi; Rustikawati, Rustikawati; Yulian, Yulian
Akta Agrosia Vol 21 No 2 (2018)
Publisher : Badan Penerbitan Fakultas Pertanian (BPFP), Fakultas Pertanian, Universitas Bengkkulu

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Abstract

Development of high yielding and superior cultivars requires information on characteristic of germplasm will be used. Ten curly-type hot pepper genotypes was grown in a completely randomized design with three replication in a low altitude land to determine their morphological characters and to evaluate genetic relationship among them based on their morphological similarities. The study was conducted in September 2015 until January 2016 in Medan Baru experimental station of Faculty of Agriculture, 15 m above sea level.  Observation was conducted on both quantitative and qualitative characters.  The results showed that there were not significantly diferent (?=5%) for plant habitus, leaf shape, leaf tip, leaf edge, leaf shape and seed shape.  Whereas for quantitative variables of stem diameter, number of fruits per plants and fruit length were significantly different, while for the other variables were not.  Cluster analysis with phylogenetic trees in 58% similarity coefficient resulted that the genotypes grouped in to six groups.  Group I consisted of genotype LPK and Ferosa, group II Mario, group III was genotype Laris, group IV consisted of genotype Kopay and Romario, group V KH and L Curup, and group VI included genotype Bogota and Sempurna. The Kopay and Romario genotypes have the closest relationship level of 73%, while Local Payakumbuh and Lokal Curup have the farrest relationship with the coefficient of 35%. From the results of the study it can be concluded that Local Payakumbuh and Local genotypes Curup can be used as parents with the highest probability to have high transgresive segregation or highest hybrid vigor. Keywords: morphological characterization, genetic relationship, curly hot pepper
Effect of Several Types and Concentrations of Complex Organic Compounds on Growth of Pineapple In Vitro Sianipar, Mangara W; Rustikawati, Rustikawati; Bertham, Yudhy H; Herison, Catur; Mukhtasar, Mukhtasar
Akta Agrosia Vol 22 No 1 (2019)
Publisher : Badan Penerbitan Fakultas Pertanian (BPFP), Fakultas Pertanian, Universitas Bengkkulu

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Abstract

Pineapple plants are usually propagated vegetatively by utilizing the base of the fruit (ratoone), stem buds (sucker), fruit buds (slip) and crown (crown). Propagation of plants by utilizing parts of the plant is less effective in meeting the needs because the planting material produced is small and requires a long time. An alternative to the propagation of pineapple to increase the amount of planting material is by tissue culture techniques. This study aims to obtain the best types of complex organic compounds and the right concentration for the growth of pineapple shoots in vitro. The research was conducted in June 2017 until October 2017 at the Laboratory of Agronomy at the Faculty of Agriculture, University of Bengkulu. Complete randomized design with 3 replications was used to allocate 9 treatments of complex organic compounds. The basic media used are the composition of Murashige and Skoog (MS). Treatment consists of 1) Coconut water 100 ml / l, 2) Coconut water 150 ml / l, 3) Coconut water 200 ml / l, 4) Banana porridge 100 gl / l, 5) Banana pulp 150 g / l, 6) Banana slurry 200 g / l, 7) Bean sprout extract 100 g / l, 8) Bean sprout extract 150 g / l, 9) Bean sprout extract 200 g / l. The results showed that at 16 weeks after MS media planting the addition of 100 g / l banana pulp was able to produce the highest plant height with an average of 3.45 cm. MS media with the addition of 200 ml coconut water resulted in the highest shoot height of 1.5 cm and followed by MS with the addition of 100 g / l media of banana pulp with an average of 0.9 cm. MS media with the addition of 100 ml / l of coconut water is suitable for the growth of pineapple roots with the average value of the number of roots and the highest length of roots. MS media with the addition of bean sprout extract with various concentrations is not able to stimulate shoot growth and has a growth response that tends to be slow. Keywords: Pineapple, In Vitro, Complex Organic Compounds.
Tolerance of 20 heirloom rice varieties at seedling stage salinity stressed and their growth in lowland coastal area Rustikawati, Rustikawati; Herison, Catur; Chozin, Muhammad; Indres, Indres
Akta Agrosia Vol 23 No 2 (2020)
Publisher : Badan Penerbitan Fakultas Pertanian (BPFP), Fakultas Pertanian, Universitas Bengkkulu

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Abstract

Coastal area of Bengkulu Province is home of many heirloom rice varieties and play an important role in contributing rice production in the province. However, as the climate change proceeds, a progressive sea water inundation has increased soil salinity mainly in low-lying areas and jeopardizing the sustainability of rice production in coastal regions.  The use of salt-tolerant varieties, therefore, would be a sensible solution to alleviate the adverse effect of soil salinity in respect of maintaining the crop production in the coastal areas. The objective of this study was to examine the salinity tolerance of 20 heirloom rice varieties collected from a coastal area of Bengkulu Province.  Prior to the screening process, lethal concentration 90 (LC90) was determined by assaying the seedlings of ‘Kuning Tinggi’ in a series of nutrient solution containing a different concentration of NaCl (0, 2000, 4000, 6000, 8000 and 10000 ppm). Using a regression analysis, LC90 was detected at a concentration of 3910 ppm. The screening was performed by exposing the varieties in the nutrient solution culture containing 4000 ppm NaCl and growing them on the tidal swamp. Under nutrient culture evaluation, the symptom of NaCl toxicity was scored and converted to salinity tolerance index. ‘Humbur’, ‘Kuning Tinggi’ and ‘Padang Bakung’ exhibited medium tolerant, while ‘Beram’, ‘Imperata’ and ‘Kuning’ exhibited very sensitive. Further evaluation of the tidal swamp for vegetative and generative performances signified that ‘Humbur’ and ‘Kuning Tinggi’ had medium tolerant to salinity stress
KORELASI DAN SIDIK LINTAS KARAKTER PERTUMBUHAN DAN KOMPONEN HASIL DENGAN HASIL PADA 15 GENOTIPE HIBRIDA JAGUNG Hellianti Pennita; Catur Herison; Marwanto Marwanto; Rustikawati Rustikawati
Jurnal Ilmu-Ilmu Pertanian Indonesia Vol 22 No 1 (2020)
Publisher : BPFP Universitas Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31186/jipi.22.1.1-8

Abstract

[PATH ANALYSIS OF GROWTH CHARACTERS AND YIELD COMPONENTS WITH THE YIELD OF 15 GENOTYPES OF MAIZE HYBRID].  Determination of traits used as the selection criteria are based on the trait(s) that have a close relationship to the yield.  The aims of this research were to measure the closeness of the relationship between morphological (growth and yield component) traits and crop yield, and to determine the trait that directly or indirectly affected on maize yield.  The research was conducted in Rimbo Kedui’s Village, South Seluma Subdistrict, Seluma District, Bengkulu Province on May 2017 to August 2017.  The experimental design used was a Complete Randomized Block Design (CRBD) in a single factor with 3 replications.  The treatments were 15 maize hybrid genotypes, consisting of CT1, CT2, CT3, CT4, CT5, CT6, CT7, CT8, CT9, CT10, CT11, CT12, CT13, CT14, and CT15.  The data were statistically analyzed by analysis of varians (ANOVA) with P(a=5%).  The qualitative data were analyzed descriptively. The result of correlation analysis revealed that the yield of maize hybrids highly correlated to some growth and yield component factors.  High-yielding maize hybrids were supported by higher ear diameter, plant fresh weight, plant dry weight, plant height, the number of seeds per ear.  Plant dry weight became the variable that has the highest direct effect with positive correlation on the yield.  The second highest rank was the number of seeds per ear with also a positive correlation value.  Yield improvement of maize hybrid, therefore, can be obtained by fostering the plant total biomass and/or by multiplying the number of seeds per ear.
Co-Authors A.W. Sandhi S.G. Adeputri, Eriana Agus Purwito Almunawar, Said Agil Alnopri Alnopri Alnopri, Alnopri Amanda, Kiki Rizki Andi Tri Maryono Angraini, Dila ARIANDANA WANTHO Atra Romeida Atra Romeida Bambang Gonggo Murcitro Bertham, Yudhy H Bilal Chairul Ahmad Deo Surya Anjaya Dewi Sukma Dival Satria Gunawan Dwi Wahyuni Ganefianti Dwi Wahyuni Ganefianti, Dwi Wahyuni Dwitama, Niko Edhi Turmudi Edi Susilo Eko Suprijono Ela Hasri Windari, Ela Hasri Windari Emilda Tri Mauli Entang Inoriah Entang Inoriah Fahrurrozi, Fahrurrozi Fernandez, Regi Hasanudin Hellianti Pennita Herison, Catur Herry Gusmara Hindarto, Kanang Setyo Indres, Indres Ineu Sulastrini Inoriyah, Entang Joko Suharjo, Usman Kris Kamil, Maulana Insanul Laksono Trisnantoro Luwih, Bagus Edi Maemunah Maemunah Marulak Simarmata Marulak Simarmata Marulak Simarmata Marwanto Marwanto Meko Gustian Merakati Handajaningsih Merakati Handajaningsih Merakati Handajaningsih Merakati Handayaningsih Merakati Handayaningsih Mimi Sutrawati Muhammad Chozin, Muhammad Muhammad Isra’ Aulia Muhammad Ravie Mukhtasar Mukhtasar Mukhtasar, Mukhtasar Nabila, Athya Kania Nadia, Nur Ain NESTI FRONIKA SIANIPAR Nurjanah, Uswatun Pardosi, Santi K. Parwito Parwito Reny Herawati Ria Meita S. H. Sutjahjo Saputra, Pringky Okta Saragih, Rosa Sri Andari Sianipar, Mangara W Sri Winarsih Sri Wulandari Sri Wulandari Wulandari, Sri Wulandari Sudarsono Suharjo, Usman Kris Joko Sukisno Sukisno Sukisno Sukisno, Sukisno Surjono H. Sutjahjo Surjono H. Sutjahjo Surjono H. Sutjahjo Surjono Hadi Sutjahjo Surmaini, Ervi Suryati, Dotti Syafira Dwi Wulandari Syafitri, Alya Novia Syakia Kurin, Aricha Umi Salamah Vera Dwisari Viona Juanda Putri Widodo Widodo Wilmar Maarisit Windari, Ela Hasri Wuri Prameswari Yulian, Yulian