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All Journal International Journal of Public Health Science (IJPHS) Buletin Peternakan Jambi Medical Journal : Jurnal Kedokteran dan Kesehatan Archive of Community Health Jurnal Sosial Ekonomi Pesisir Jurnal Sain Peternakan Indonesia Jurnal Kesehatan Masyarakat Andalas (Andalas Journal of Public Health) Jurnal Peternakan Indonesia Jurnal Berkala Epidemiologi Jurnal Promkes: The Indonesian Journal of Health Promotion and Health Education JOURNAL OF HEALTHCARE TECHNOLOGY AND MEDICINE Jurnal Ekonomi dan Bisnis Islam Aceh Journal of Animal Science Jurnal RESTI (Rekayasa Sistem dan Teknologi Informasi) Emerging Science Journal Jukema (Jurnal Kesehatan Masyarakat Aceh) Jurnal Penelitian Pendidikan IPA (JPPIPA) Jurnal Kesehatan Medika Saintika FIKRAH International Journal of Community Service Learning Buletin Ilmiah Nagari Membangun JIK- JURNAL ILMU KESEHATAN Health and Medical Journal Contagion: Scientific Periodical Journal of Public Health and Coastal Health Jurnal Masyarakat Maritim Warta Pengabdian Andalas: Jurnal Ilmiah Pengembangan Dan Penerapan Ipteks Bulletin of Computer Science Research Semah : Jurnal Pengelolaan Sumberdaya Peraira Jurnal Pengabdian Inovasi dan Teknologi Kepada Masyarakat Tadbiruna: Jurnal Manajemen Pendidikan Islam Jurnal Pengabdian Masyarakat Gemilang (JPMG) Jurnal Pengabdian Masyarakat Bhinneka Depik Jurnal Ilmu-Ilmu Perairan, Pesisir dan Perikanan Asian Multidisciplinary Research Journal of Economy and Learning Prosiding Seminar Kesehatan Nasional International Journal of Islamic Business and Economics (IJIBEC) Journal of Contemporary Indonesian Islam (JCII) Fikrah : Jurnal Pendidikan Agama Islam
Masrizal Masrizal
Andalas University
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Journal : Buletin Peternakan

 1 
Evaluation of Different Techniques in Recovering of Oocytes and Storage Duration of Ovaries on the Quality and Quantity of Bovine in Vitro Maturation Zaituni Udin; Masrizal Masrizal; Hendri Hendri; Syafri Nanda
Buletin Peternakan Vol 44, No 2 (2020): BULETIN PETERNAKAN VOL. 44 (2) MAY 2020
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v44i2.52181

Abstract

This study aimed to evaluate different recovery techniques of oocytes and the duration time between ovary collection and processing in the laboratory on the quality and quantity of Simmental cross bovine oocytes in vitro maturation. A total of 75 bovine ovaries were divided into three groups. Experiment-1;  the storage duration of ovaries were 6, 9 and 12 h. Experiment -2;  the techniques for oocytes recovery were slicing, aspiration and slicing + aspiration. The ovaries were collected from slaughterhouse and kept in saline solution during transportation to laboratory. A total of 136 oocytes were used for experiment-1, and 246 oocytes for experiment-2. This study found that the number and quality of oocytes were significantly different (P<0.05) as the effect of storage time. The average number of oocytes were 15.90±2.48; 17.10±1.38 and 13.60±3.00, in 6 h; 9 h and 12 h of storage time, respectively. The techniques of oocytes collection significantly (P<0.05) affect the quantity of oocytes but no significant (P>0.05) on oocytes quality. The average of oocytes of groups slicing, aspiration and slicing plus aspiration were 14.66±2.09; 9.46±2.99 and 16.40±6.86, respectively. Both experiment was significant effect (P<0.05) on immaturation rate of bovine. Conclusion of this study the storage time of ovaries was suitable in 9 h and the technique of oocytes collection was in slicing plus aspiration.
Co-Authors Abdul Rahman Ade Chandra Ade Suzana Eka Putri Ahmad Fachrurrozi Aisyah alfiza rahmanita Amat Sofiyan Annur Azira Rosi Arie Linarta Arinil Haq Arlius Arlius Ayu Salsabila Ayu Sapitri Azhar Azhar Bambang Bambang Bari, Salsabila Unaya Besral . Budi Trianto Deasy Wahyuni Deci Irmayani Defra Monika Devy Shintya Dewi Nur Aisyah Diastanti, Yunita Eka Budi Satria Elsha Lukman Elvina Evan Hamzah Muchtar Fadilah Maysarah Nasution Fahmi Rizal Fauzan Fauzi, Falah Fitri, Juli Hati Fitria Suherni Fivi Melva Diana, Fivi Melva Fritiara Fritiara Gabriella Gebrina, Deanne Putri Gomal Juni Yanris Hari Kusnanto Hayatullah Khumaini Hendri Hendri Hendri Hendri Hendri Sayuti Heni Wiyandari Ibnu Phonna Nurdin, Ibnu Phonna Ibnu Rasyid Munthe Imalatul Mardhiyah Imraatul Hasni Indri Apriyani Irmayanti Irpan Isniati Jaswandi . Jendrius Jendrius Jenita Sari Juledi, Angga Putra Juli Hati Fitri Khaira Fillaili Khairul Rizal Lubis, Arga Abdi Rafiud Darajat Luthan Lazuardi, Luthan Mahesa Mahesa Mahesa Mahesa Mardiah Putri Al Ghani Marnis Nasution Mas Eriza Misri A. Muchsin Muhammad Alfarezi Muhammad Halmi Dar N. Rahma Nova Permata Sari Novnariza, Elsi Perwati, Ane Dayu Putra, Meiyaldi Eka Putri Erwina Putri Rahmadani, Putri Putri, Sri Mardhiani Penta Rahmi Tanesha Raisyifa Raisyifa Rambe, Inny Rahayu Rasyid Munthe, Ibnu Ratno Widoyo, Ratno Reflita Reflita Ridar Hendri Rizanda Mahcmud Rizanna Rosemary Rizky Haditya Rosfita Rasyid Rusli Yusuf Sabaruddin Satia Negara Lubis Selia Juwita Shohibul Azmi Sri Hardian Sri Siswati Suryadi, Sudi Syafrawati Syafri Nanda Syamsurizal Tasiu Tijjani Sabiu Tengku Iqbal Pradinata Tria Syananda Putri Usman Bulanin Usman Bulanin Vitratul Ilahi Vitratul Ilahi WAHYU FIRMANSYAH Wahyu Rahman Wardi Wenny Delma Yati Yeffi Yeffi Masnarivan Yeka Hendriyani Yessy Markolinda Yudi Yudi Yusmaidar Sepriani Z. Udin Zaimsyah, Annisa Masruri Zainal A. Muchlisin Zaituni Udin Zebua, Yuniman Zuhri Harahap, Syaiful