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Periodic Fasting Treatment on Growth Performance and Feed Efficiency of Nile Tilapia (Oreochromis niloticus, GIFT) Jacob Da Costa Pereira; Salustiano Zaret; Mateus Salvador; Kismiyati; Sapto Adriyono; Ahmad Shofy Mubarak; Mohammad Anam Al Arif
Journal of Basic Medical Veterinary Vol. 15 No. 1 (2026): Journal of Basic Medical Veterinary, June 2026
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v15i1.81125

Abstract

Fasting is a feed management strategy that reduces feeding frequency without inhibiting fish growth. This strategy involves specific intervals of feed deprivation, allowing fish to exhibit increased appetite once feeding resumes. This study aimed to evaluate the effects of periodic fasting on the growth performance and feed efficiency of GIFT Nile tilapia, as well as to identify the most effective fasting pattern for optimal results. The research used a completely randomized design (CRD) with four treatments, each replicated three times: continuous feeding without fasting (K, control); two days of feeding followed by one day of fasting (2E1F); three days of feeding followed by one day of fasting (3E1F); and four days of feeding followed by one day of fasting (4E1F). The primary parameters observed were absolute weight gain (g), absolute length growth (cm), and feed efficiency. Data were analyzed using the Kruskal–Wallis test (p < 0.05), followed by post hoc comparisons using independent samples tests to determine significant differences among treatments. The results showed that periodic fasting had a significant effect (p < 0.05) on absolute weight gain, absolute length growth, and feed efficiency of GIFT Nile tilapia. The best performance was observed in treatment 4E1F (four days of feeding followed by one day of fasting), while the lowest performance occurred in treatment 2E1F (two days of feeding followed by one day of fasting).
Pengaruh Konsentrasi Protein yang Berbeda dalam Suspensi Dedak sebagai Pakan terhadap Diameter Kista Artemia salina : Pengaruh Konsentrasi Protein yang Berbeda dalam Suspensi Dedak sebagai Pakan terhadap Diameter Kista Artemia salina Ahmad Shofy Mubarak; Laksmi Sulmartiwi; Muhammad Daffa Al Rasyid Nurhayati
Journal of Aquaculture Science Vol 8 No 1 (2023): Journal of Aquaculture Science
Publisher : Airlangga University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31093/joas.v8i1.278

Abstract

Artemia salina is a natural food that is very important in hatcheries. The need for Artemia as a natural feed for fish or shrimp seeds is high in Indonesia, so it is necessary to develop local Artemia cultivation. One way to do this is to increase the production of Artemia salina cysts by paying attention to the quality and quantity of feed, namely enrichment with ISP (Isolated Soya Protein) flour in the feed given. ISP is a protein isolated from soybeans with the final product in the form of flour, easy to digest and has high nutritional value. Enrichment with ISP flour was carried out with the aim of increasing the protein content that affects the formation of the shell and the size of the good Artemia salina cyst. This study aimed to determine the effect of different protein concentrations using ISP (Isolated Soya Protein) in bran suspension on the diameter of Artemia salina cysts. The research method used was experimental with a completely randomized design (CRD) using 5 treatments and 4 replications. The results showed that different protein concentrations by enriching ISP flour (Isolated Soya Protein) in bran suspension as feed had a significant effect (P<0.05) on Artemia salina cyst diameter in the third week. The administration of ISP with a protein concentration of 40% and 45% showed the highest Artemia salina cyst diameter.
Individual Growth And Gonad Development Of Female Tilapia (Oreochromis Sp.) With Addition Of Velvet Bean (Mucuna Pruriens) Seed Extract Ahmad Shofy Mubarak; Kamiliya Zahrah Taher; Putri Eriza Riyanto; Akhmad Taufiq Mukti; Muhamad Amin; Syifania Hanifah Samara
Journal of Aquaculture Science Vol 8 No 2 (2023): Journal of Aquaculture Science
Publisher : Airlangga University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/joas.v8i2.48698

Abstract

Tilapia is one of the fish that is widely cultivated in Indonesia. The obstacle to tilapia cultivation is high reproduction, which inhibits growth. An alternative to increase growth is by inhibiting gonad growth, so that feed energy is focused on somatic development. The aim of this research was to analyse the effect of velvet bean extract concentration on individual growth and gonad development of female tilapia fish and to determine the optimum concentration of velvet bean extract to increase growth and reduce reproductive activity in female tilapia fish. This study used a Completely Randomized Design (CRD) with the addition of various velvet bean extracts and compared with the control (without velvet bean extract). The use of velvet bean extract had a significant effect (p<0.05) on growth parameters such as length, weight, fecundity and egg diameter. The best treatment was found in treatment 3 (velvet bean extract 5 ml.kg-1 ) which resulted in growth in individual length of 16.1 cm, individual weight of 89.43 g, fecundity of 2206 and egg diameter of 1.11 mm. In addition, the dose in this treatment may reduce the reproductive activity of tilapia.
Konsentrasi Madu Sebagai Pelapis Pada Penyimpanan Kering Terhadap Daya Tetas Ephipia dan Kelangsungan Hidup Naupli Moina macrocopa Kinanthi Sajda Tsabita; Ahmad Shofy Mubarak; Nina Nurmalia Dewi
Journal of Aquaculture Science Vol 9 No 1 (2024): Journal of Aquaculture Science
Publisher : Airlangga University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/joas.v9i1.48703

Abstract

Moina macrocopa is natural feed that commonly found in tropical waters. Ephippia can be stored and hatched at any time. Embryo stability can be protect by controlling the water content through coating technology. Honey solution can be used as a coating that have a osmotic regulation for prevent embryo from drought while ephipia entering the dormant stage. This research aims to determine the effect of concentration on the hatchability of ephipia and the survival of M. macrocopa nauplii. The research method used in this study was experimental with a completely randomised design (CRD) using five treatments and four replications. The results showed that coating ephipia using honey had a significantly different effect on the hatchability of M. macrocopa ephipia (p<0,05). However, the concentration of honey was not significantly different on the survival of M. macrocopa nauplii. Keywords : Ephipia, Honey, Moina macrocopa, Coating
The Influence of Different Concentrations of Isolated Soy Protein (ISP) Powder in Rice Bran Suspension as Feed on the Production of Artemia salina Cysts Marwah Sifa Safira Mosyafwa; Ahmad Shofy Mubarak; Laksmi Sulmartiwi
Journal of Aquaculture Science Vol 10 No 1 (2025): Journal of Aquaculture Science
Publisher : Airlangga University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/joas.v10i1.49159

Abstract

Artemia salina is a natural food source known for its high nutritional quality. It is commonly used in larval rearing due to its ability to produce long-lasting cysts that can hatch at any time. These characteristics make A. salina very suitable for sustainable cultivation. In this study, the feed consisted of rice bran suspension enriched with Isolated Soy Protein (ISP) meal. The aim was to investigate the effect of different protein concentrations in the rice bran suspension feed on cyst production in A. salina broodstock. This study used a Completely Randomized Design (CRD) with five treatments and four replications. The treatments included P0 (without ISP meal, 25% protein concentration), P1 (ISP meal, 40% protein concentration), P2 (ISP meal, 45% protein concentration), P3 (ISP meal, 50% protein concentration), and P4 (ISP meal, 55% protein concentration). Data analysis was performed using ANOVA followed by Duncan's multiple range test. The results showed that variations in protein concentration with the addition of ISP flour had a significant effect (P < 0.05) on total cyst production and cyst production per broodstock of A. salina. The highest total cyst production (710.67 cysts/L) was achieved in P4 with a protein concentration of 55%, while the highest cyst production per broodstock (28.67 cysts/broodstock) occurred in P2 with a protein concentration of 45%.
Artificial Seawater Use of Bittern Minerals on the Survival and Growth of Vannamei Shrimp (Litopenaeus vannamei) and Potential Resources Rr Sonia Rahmania Sonjaya; Mayvita Nur Faizah; Rizka Tri Puji Haryanti; Khansa Ilma Nafi'ah; Nadia Priyandra Laksmidewi; Ahmad Shofy Mubarak; Muhamad Amin; Yasha Husnacahya
Journal of Aquaculture Science Vol 10 No 2 (2025): Journal of Aquaculture Science
Publisher : Airlangga University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/joas.v10i2.61984

Abstract

Whiteleg shrimp (Litopenaeus vannamei) cultivation can be carried out in areas far from the sea with the RAS (Recirculating Aquaculture Systems) system using artificial seawater (ASW) added with bittern as a mineral source. This study aimed to determine the effect of artificial seawater formulation using bittern minerals on growth, survival, glucose levels, and hepatopancreas histology in whiteleg shrimp. This study used a completely randomized design (CRD) with 5 treatments and 4 replications including (P0) seawater; (P1) 18.56 grams of crossover salt + 8.4 ml/liter of bittern; (P2) 14.93 grams of crossover salt + 16.86 ml/liter of bittern; (P3) 11.53 grams of crossover salt + 25.13 ml of bittern/liter; (P4) 23 grams of crossover salt/liter. This study began with the crystallisation and characterisation of bittern, continued with mineralisation tests, and prepared artificial seawater using a combination of salt and bittern solutions. Cultivation was carried out for 20 days, and then survival, growth in length and weight, water quality, blood glucose, and histology of hepatopancreas tissue were observed. The formulation of artificial seawater treated with P1 was the best, with a survival rate of 78% and growth no different from control seawater. Therefore, artificial seawater has the potential to be used for the cultivation of whiteleg shrimp.
KOMBINASI NANOPARTIKEL KITOSAN DAN MODIFIED TAPIOCA-WHEY PROTEIN DALAM PEMBUATAN EDIBLE COATING TERHADAP AKTIVITAS ANTIBAKTERI DAN ORGANOLEPTIK OTAK-OTAK TENGGIRI Farras Rana Hafizhah; A. Shofy Mubarak; Gunanti Mahasri
Journal of Aquaculture Science Vol 9 No 2 (2024): Journal of Aquaculture Science
Publisher : Airlangga University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/joas.v9i2.62039

Abstract

Product otak-otak ikan tenggiri is a promising fishery diversification product but is prone to spoilage. Edible coatings can help slow down spoilage. The purpose of this study was to determine the effect of different edible coating formulations on the characteristics of edible coating and to determine the protective potential of edible coatings against bacterial growth and organoleptic product otak-otak ikan tenggiri during storage. This research is an experimental study that is divided into two stages, the first stage is the characteristics of edible coatings using a completely randomized design (CRD) with testing including antimicrobial activity, water absorption, and viscosity with 5 treatments and 4 replicates, and the second stage is coating applications on the length of storage of product otak-otak ikan tenggiri testing including TPC and organoleptic using two-factor CRD 3×6 treatments and 3 replicates. Results indicated a significant impact (P < 0.05) of coating formulations on these characteristics. The best formulation, modified tapioca-WPI with CSNP (P4), showed antibacterial activity with a 9.16 mm inhibition zone, 3.46% water absorption, and 15.33 cP viscosity. This formulation maintained microbial stability and organoleptic quality for up to 3 days at room temperature and 12 days under cold temperature.
KOMBINASI NANOPARTIKEL KITOSAN DAN TURUNAN SELULOSA DALAM PEMBUATAN EDIBLE COATING TERHADAP AKTIVITAS BAKTERI DAN ORGANOLEPTIK PEMPEK IKAN TENGGIRI Fadiah Shabrina; Gunanti Mahasri; A. Shofy Mubarak
Journal of Aquaculture Science Vol 9 No 2 (2024): Journal of Aquaculture Science
Publisher : Airlangga University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/joas.v9i2.62040

Abstract

Pempek is a very popular traditional Indonesian food because it has a delicious taste. However, the shelf life of pempek is only about 16 hours at room temperature. This study aims to develop and evaluate edible coatings based on cellulose and chitosan nanoparticles (CSNPs) to extend the shelf life of pempek from Tenggiri fish (Scomberomorus commerson). This study is experimental research divided into two stages. The first stage involves characterizing the edible coating using a Completely Randomized Design (CRD), with tests including antimicrobial activity, water absorption, and viscosity, conducted with 5 treatments and 4 repetitions. The second stage involves applying the coating to pempek and evaluating its shelf life, focusing on TPC and organoleptic qualities using a two-factor CRD with 3×6 treatments and 3 repetitions. The results showed a significant effect (P<0.05) of various formulations on the characteristics of edible coatings. The results showed that the variation of cellulose derivatives and CSNP in the preparation of edible coating significantly affected the coating characteristics (P<0.05). The combined formulation of CMC and CSNP (P5) was selected as the pempek coating, with antibacterial activity of 9.31 mm, water absorption of 1.23%, and solution viscosity of 33.50 cPa.s. In the second stage, the treatment of pempek coating with CMC and CSNP (C2) effectively maintained the quality of pempek until day 3 at room temperature and day 12 at refrigerator temperature.
Stress Response and Pathology of Hybrid Groupers (Epinephelus lanceolatus × Epinephelus fuscoguttatus) Infested Trichodina sp. Maintained in Hatchery from Bali, Indonesia Lia Oktavia Ika Putri; Gunanti Mahasri; Ahmad Shofy Mubarak; Lilis Cahaya Septiana
Journal of Aquaculture Science Vol 10 No 1 (2025): Journal of Aquaculture Science
Publisher : Airlangga University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/joas.v10i1.67885

Abstract

The demand for grouper fish continues to increase, with the Ministry of Fisheries' annual production target of 9,000 tons. However, intensive cultivation systems pose risks due to poor water quality, making grouper fish susceptible to disease and health problems. This study investigated the impact of Trichodina sp. infection on the blood glucose levels of hybrid grouper fish. The study was conducted in May 2024 by observing 30 fish and measuring blood glucose. The results showed that 20 fish showed glucose levels above the normal range (> 57.6 mg/dL), ranging from 61 to 114 mg/dL. In contrast, nine fish had glucose levels in the normal range (28.8 - 57.5 mg/dL), and one fish had low glucose levels of 26.7 mg/dL. High glucose levels in most fish indicate stress or metabolic disorders, possibly related to Trichodina sp. infestation. This parasitic infection can compromise the fish's immune system, making the fish more susceptible to disease. The results emphasize the need for effective parasite management and optimal water quality in intensive culture systems. To maintain grouper health and productivity, it is important to monitor water quality, control ectoparasites, and conduct further research on the long-term effects of these infections on blood glucose and overall health.
Co-Authors A. Taufiq Mukti A.Taufiq Mukti Abdillah, Annur Ahadi Adde Ermawan Akhmad Taufiq Mukti Akhsan Panna Al arif, Mohammad Anam Alfi Hermawati W Amilaningrum, Diadara Amin, Muhamad Nur Ghoyatul Annisa Nur Rohmah Purnamasari Apriana Vinasyiam Arum, Kartika Dwi Sekar Boedi Setya Rahardja Browijoyo Santanamurti Cahyani Prasetyawati, Fatimah Carattri, Money DARMAWAN SETIA BUDI DEDI JUSADI Desi Nawang Purnamasari Destya Twinandia Diah Ayu Satyari U Diah Trie Ridyaning Tias Diana Meritasari Djamil, Hasim Dwi Yuli Pujiastuti Eka Ainurrohmah Eka Saputra Eka Saputra Endang Dewi Masithah Endang Tri Wahyurini Fadiah Shabrina Faricha Risma Nurani Farras Rana Hafizhah Fasya, Arif Habib Fitrawan, M. Dzaky Fransisca, Fida Gunanti Mahasri Heru Pramono Husein, Mohamad Akmal Alwi Intan Cahyarini Isroni, Wahyu Jacob Da Costa Pereira Juanda Juanda Julie Ekasari Kamiliya Zahrah Taher Kamiliya Zahrah Taher Kenconojati, Hapsari Khansa Ilma Nafi'ah Kinanthi Sajda Tsabita Kismiyati , Lailatul Lutfiyah, Lailatul Laksmi Sulmartiwi Layuk, Tresia Sanda Lia Oktavia Ika Putri Likanimasayu Prabandari Lilis Cahaya Septiana M. Zairin Junior Marwah Sifa Safira Mosyafwa Mateus Salvador Maulida Rosa Umainana Maulidia, Cici Mayangsari, Cholivia Mayvita Nur Faizah Melani Indah Sari Manik Muhamad Amin Muhamad Amin MUHAMMAD AGUS SUPRAYUDI Muhammad Daffa Al Rasyid Nurhayati Müller, Tamás Mulyono Mulyono Munjayana, Munjayana N. Juni Triastuti Nadia Priyandra Laksmidewi Nina Nurmalia Dewi Ninin C Nurmalia Dewi, Nina Patmawati Patmawati, Patmawati Permana Sulistyo Rini Pursetyo, Kustiawan tri Puspitasari, Febriyanti Putri Alia Paramitha Putri Eriza Riyanto Putri, Monica Angelina R. Rahardyan Prasetyo Rahayu Kusdarwati Rahmadani Rahmadani Rahmasari, Naila Dwi Ramadhan, Novelix Arditan Ratna Diyah Palupi Reni Oktaviani Restanti, Maya Rizka Tri Puji Haryanti Rochmatika, Endah Rohmatin, Aulia Rokhim, Akhmad Nur Rr Sonia Rahmania Sonjaya Rr. Juni Triastuti Sahidu, Adriana Monica Sahidu, Andriyana Monica Salustiano Zaret Samara, Syifania Hanifah Sapto Adriyono Sapto Andriyono Saragih, Friska Setiawani Sari, Devi Elvina Septuresty Hartri Eka Setyantini, Woro Hastuti Setyawati Sigit Sihombing, Agusmanto Sri Subekti Steffani Lutfi Yuni firdausi Suciyono, Suciyono Sudarno, Sudarno Utari, Heny Budi Veryl Hasan Widigdo, Syafrianur Win Darmanto Wiyoto Wiyoto Woro H. Satyantini Woro Hastuti Satyantini Wulan, Novrida Yasha Husnacahya Yeni Dhamayanti Yuli Pujiastuti, Dwi Yusuf Taufik Hidayat Zurica Melati Fitri