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PRELIMINARY LABORATORY EVALUATION OF RAPID DIAGNOSTIC TEST PROTOTYPE TO DETECT HBsAg IN WHOLE BLOOD AND SERUM Qurnianingsih, Ema; Wungu, Citrawati Dyah Kencono; Amin, Mochammad; Setyawati, Dewi; Yasan, Yanna Debby Restifanny; Lukitasari, Lina; Lusida, Maria Inge; Soetjipto, Soetjipto
Folia Medica Indonesiana Vol. 61, No. 2
Publisher : Folia Medica Indonesiana

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Abstract

Chronic hepatitis B virus (HBV) remains a major global health issue,[-10pc]AU: Please provide ORCID ID for all authors.[-7pc]AU: Please provide affiliation’s for all authors. with over 250 million people affected worldwide. In Indonesia, HBV prevalence has declined from high to moderate endemicity, but challenges persist due to regional disparities, risk of mother-to-child transmission, and the predominance of B3 and C1 sub-genotype. Despite available preventive measures and diagnostic tools, approximately 90% of cases remain undiagnosed, particularly in low-resource settings, indicating the need for improved screening and control strategies. Therefore, this study aims to conduct preliminary performance tests of KODC hepatitis B surface antigen (HBsAg) (rapid diagnostic test (RDT) prototype) produced by Konimex on serum and whole blood samples. Performance was assessed through sensitivity, precision, specificity, and comparative testing against commercial RDT. The results obtained were visually interpreted by 2 independent readers. KODC HBsAg RDT demonstrated a detection limit of 50 IU/ml in serum and 400 IU/ml in whole blood. Precision testing showed high reproducibility (RSD <3.02%), with no cross-reactivity against other pathogens. Compared with a commercial RDT, it showed equivalent performance in serum and superior specificity in whole blood. In addition, predictive value analysis confirmed excellent accuracy, with Positive predictive value (PPV) and negative predictive value (NPV) >99% in both sample types. Therefore, KODC HBsAg RDT prototype showed comparable performance to commercial tests with good sensitivity, precision, and specificity, indicating its potential as a practical tool for HBV screening in resource-limited settings.
Improving Posyandu Cadres knowledge through stunting education and training on sea fish–based MPASI and PMT preparation in Darungan Village Ira Humairah; Patmawati Patmawati; Ema Qurnianingsih; Lina Lukitasari; St. Khaerunnisa; Fithriyah Cholifatul Ummah; Langgeng Agung Waskito; Citrawati Dyah Kencono Wungu; Imanta Alifia Oktavira; Novita Nur Ramadhani; Dede Sayyidatunnisa; Muhammad Hidayat; Gwenny Ichsan Prabowo
Buletin Pengabdian Bulletin of Community Services Vol. 6 No. 1 (2026): Bull. Community. Serv.
Publisher : LPPM Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/bulpen.v6i1.2

Abstract

Background and Aim: Stunting remains a major public health problem that affects child growth and development. Community-based nutritional interventions utilizing local food resources are important strategies to support stunting prevention. This community service activity aimed to improve the knowledge and skills of Posyandu cadres regarding stunting prevention through education and training on the preparation of sea fish–based MPASI and PMT in Darungan Village. Methods: The program was conducted using educational counseling, practical training, and mentoring activities for Posyandu cadres. Participants received materials on stunting prevention, balanced nutrition, and practical demonstrations of preparing sea fish–based MPASI and PMT. Knowledge improvement was evaluated using pre-test and post-test assessments. Results: The results showed a significant increase in participants’ knowledge regarding stunting prevention and balanced nutrition, with a p-value of 0.025 (p < 0.05). In addition, participants demonstrated improved practical skills in preparing nutritious sea fish–based complementary and supplementary foods for children. Conclusion: Education and training using locally available sea fish as nutritional resources effectively improved Posyandu cadres’ knowledge and practical skills in stunting prevention. This program has the potential to support sustainable community-based nutrition interventions and contribute to achieving Sustainable Development Goal (SDG) 3, namely Good Health and Well-being.