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SHAKING RED CAP-BLOOD COLLECTION TUBE WITHOUT ADDITIVE SUBSTANCES IS RECOMMENDED TO ACCELERATE THE BLOOD CLOTTING PROCESS Gilang Nugraha; Rohayati Rohayati
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 1 No 1 (2019): The Value, Importance, and Oversight of Health Research
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v1i1.913

Abstract

Many blood collection tube manufacturers do not recommend shaking the red tube. Shaking the red tube to avoid the intensity of the changed of blood that interacts with the glass surface of the tube will trigger the contact path in the coagulation cascade. Generally, the blood takes 30-60 minutes to clots at room temperature without shaking. The aim of this study was to determine the effectiveness of shaking the red-cap blood collection tube in producing serum volume. The method of this study was experimental As much as 5 mL of blood was taken and put into 3 tubes with a volume of 1mL each tube. The first and the second tube were shaken 8 times. The first tube was incubated for 10 minutes while the second tube was incubated for 25 minutes. Meanwhile, the third tube (as a control) was not shaken but was incubated for 40 minutes. The tube were centrifuged at 3000 g for 10 minutes. The serum volume was measured using micropipette and collected into Eppendorf tube. The results showed that there were a difference in the number of serums formed after tube shaking by time variation (P = 0.002), the results of the Post Hoc Test using Bonferroni test while showed that the second tube did not have a difference in serum volume with control (P = 0.751). It can be concluded that the red-cap blood collection tube, which was shaken 8 times and incubated for 25 minutes long could accelerate the coagulation process.
STUDY OF HEMOGLOBIN LEVELS ON HEMOLYSIS SAMPLE Noor Febryani; Ikke Nanda Amalia; Intan Dwi Anggraeni; Gilang Nugraha
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 1 No 2 (2019): Laboratory Analysis Applied to Medical Properties
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v1i2.1311

Abstract

Hemolysis can significantly affect the reliability of test results and occur in the pre-analytical phase. The aim of this study is to reveals the correlation of hemoglobin levels on hemolysis sample. This experimental study was carried out using samples from thirty students of Medical Laboratory Technology study program of the Nahdlatul Ulama University of Surabaya. Blood samples were treated by hard shaken in 30 times in order to damage the middle part of the blood sample. Data on hemoglobin levels were collected and analyzed using the Pearson Correlation Test before and after treatment with significant value p < 0.05 indicating that there was a significant correlation. Hemoglobin concentrations were strongly positive for the hemolysis of the sample (p = 0.000). The conclusion is that sample hemolysis has a potency to be the confounding factor on the hemoglobin test.
Improving Public and Environmental Health Through Rheumatoid Screening and Education on Environment Pathogenic Fungal Contamination Suprapto Maat; Endah Prayekti; Gilang Nugraha; Vania Nur Ramadani; Winda Melindah; Imraatul Mukarramah
Indonesia Berdaya Vol. 7 No. 1 (2026)
Publisher : UKInstitute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47679/ib.20261411

Abstract

People working in agricultural environments with high humidity levels are at greater risk of exposure to pathogenic fungi, which can trigger infections of the skin, nails, and respiratory tract, especially in individuals with weakened immune systems. On the other hand, the elderly are vulnerable to health decline, including autoimmune diseases such as rheumatoid arthritis (RA), which can cause joint inflammation, tissue damage, and progressive disability. This community service activity aims to increase public knowledge about the dangers of pathogenic fungal contamination and to conduct early detection of RA through Rheumatoid Factor (RF) testing. Education was delivered interactively using lectures, discussions, and visual media, accompanied by pre- and post-tests, as well as RF testing using the latex agglutination method. This community service activity was attended by 33 participants. Results showed an increase in knowledge from 58% to 91% after the education, with all 33 RF test participants receiving non-reactive results. This program demonstrates that the combination of education and simple testing can increase public preventive awareness and is suitable for adaptation in other areas with similar risk characteristics.
Correlation Between hs-CRP and Erythrocyte Sedimentation Rate in Active Smokers Emma Ismawatie; Yoki Setyaji; Yulita Maulani; Gilang Nugraha; Cony Chori
Indonesian Health Issue Vol. 5 No. 2 (2026): AUGUST
Publisher : Indonesian Journal Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47134/inhis.v5i2.172

Abstract

Background: Smoking is a major risk factor for chronic inflammation that plays a crucial role in the development of degenerative diseases, particularly cardiovascular disorders. Inflammatory responses induced by cigarette smoke exposure can be assessed using sensitive inflammatory biomarkers such as high sensitivity C-reactive protein (hs-CRP) as well as nonspecific inflammatory parameters like the erythrocyte sedimentation rate (ESR). Objective: This study aimed to analyze the correlation between hs-CRP levels and ESR values among active smokers at PT. ASA Surabaya. Methods: This study employed an analytical cross-sectional design and was conducted at Parahita Laboratory, Surabaya, from April to May 2022. The study sample consisted of 30 active smokers selected using purposive sampling techniques. Data were collected through laboratory examinations of hs-CRP and ESR from blood samples. Statistical analysis was performed using the Spearman correlation test to evaluate the relationship between the two variables. Results: The findings showed that the majority of respondents had hs-CRP levels greater than 3 mg/dL, indicating a high-risk category (90%), while most respondents had ESR values within the normal range (73.33%). Statistical analysis revealed a p-value of 0.792 (p>0.05), indicating no significant correlation between hs-CRP levels and ESR values among active smokers. Conclusion: Elevated hs-CRP levels in active smokers are not consistently accompanied by increased ESR values, suggesting that hs-CRP is more sensitive in detecting subclinical inflammation than ESR. Therefore, hs-CRP is recommended as a more reliable inflammatory biomarker for active smokers, and further studies with larger sample sizes are needed to confirm these findings
Co-Authors Abdul Muhith Abraham Ahmad Ali Firdaus Ahmad Jazuly Nabil Akhmad Rizal Effendi Alfia, Shofa Alya Salsabila Andini, Ary Andreas Putro Ragil Santoso, Andreas Putro Ragil Anita Rizki Rahmawati Asror Ardyarini Dyah Savitri Ariska Widya Arsya Tazkiya Atika Nur Laila Aulia, Ilmiah Alvi Nisa Ayu Slatim Maifanda Ballihgoo, Hamiduumajid Ballihg Bastiana Bermawi Bermawi, Bastiana Brina Thursina Dibiasi Cony Chori Devyana Dyah Wulandari Diyan Wahyu Kurniasari Dwi Handayani Dwi Lestari Pertiwi Dwijayanti, ira Edhi Rianto Edijanto, Soebagijo Poegoeh Edijanto, Subagijo Poegoeh Effendi, Akhmad Rizal Eka Indah Murdiningsih Emma Ismawatie Ersalina Nidianti Fakurazi, Sharida Harri Darmawan Hidayat, Muhammad Taufiq Ida Lestari Ikke Nanda Amalia Ilmiah Alvi Nisa Aulia Imraatul Mukarramah Intan Dwi Anggraeni Ira Dwijayanti Ira Hasianna Rambe Janetra Yudhis Nata P Kurniasari, Diyan Wahyu Laila, Atika Nur Layla Agustina, Nurul Ivada Maat, Suprapto Maritza, Phasa Shoofii Luthf Marselli Widya Lestari Maulana Bagas Syaifulloh Melindah, Winda Mirshall Abiedama Prayoga Moch. Sahri Mohammad Nasir Muhamad Damar Baskara Muhammad Afwan Romdloni Muhammad Nasir Muhammad Rizqi Cahyadi Muhammad Yusuf Ramadhani Hakim Mukarramah, Imraatul Nastasya Nunki Nathalya Dwi Kartika Sari Navis, Khiliah Nila Dwi Utami Noor Febryani Nugraha, Dayu Satria Nur Anita Ningsih Nur Masruroh Nur Masruroh Nur Masruroh, Nur Nur Muhamad Nuzulul Syufi Nur Zalsabila Isnaini Nurul Hidayatih Permana, Indra Dafa Tia Prayekti, Endah Putri, Dwi Yuniar Nandita Rachmayanti, Notrisia Rahayu Anggraini Rahayu, Mita Ramadani, Vania Nur Rizka Wahyu Safitri Rizki Nurmalya Kardina Robert Aditya Wijanarko Rohayati Rohayati Rosa Sastrawati Rosda Rodhiyana Rodhiyana Ruri Arinil Khoiro S. P. Edijanto Penulis Salimi, Syafrina Maulidyatus Salman Firmansyah Salsabila , Khairun Nisya Salsabila, Alya Salsabillah Karunia Sugiarto Saumia Khoirunnisa Syadzila Siti Husnul Khotimah Siti Rosa Lina Sitti Fitria Soebagijo Poegoeh Edjianto Sri Surya Suciati Subagijo Poegoeh Edijanto Suciati, Sri Surya Sumarsono, Thomas Suprapto Maat Syadzila, Saumia Khoirunnisa Syinta Nur Isnaini Thomas Sumarsono Titik Sulifah Umi Hanik Utami, Nila Dwi Vania Nur Ramadani Widya Novita Sari Wieke Sriwulan Winda Melindah Wulan, Wieke Sri Yoki Setyaji Yulita Maulani Zalfa, Anisatuz