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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Majalah Obstetri dan Ginekologi Majalah Kesehatan FKUB Medical Laboratory Technology Journal Health Notions Indonesian Journal of Obstetrics and Gynecology (Majalah Obstetri dan Ginekologi Indonesia) JKM (Jurnal Kebidanan Malahayati) MMJ (Mahakam Midwifery Journal) JOURNAL LA MEDIHEALTICO Devotion: Journal of Research and Community Service Asian Journal of Health Research Jurnal Info Kesehatan
Tatit Nurseta
Department Of Obstetric And Gynecology, Faculty Of Medicine, Universitas Brawijaya, Malang
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Dentistry Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health Veterinary

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Cowpea (Vigna unguiculata) Extract Reduce Malondialdehyde Levels and Prevent Aortic Endothelial Cell Decline in Ovariectomized Rats Ni Putu Sri Haryati; Elisa Danik Kurniawati; Tanti Tri Lestary; Eviana Norahmawati; I Wayan Arsana Wiyasa; Dwi Yuni Nur Hidayati; Tatit Nurseta
Medical Laboratory Technology Journal Vol. 7 No. 2 (2021): December
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (484.221 KB) | DOI: 10.31964/mltj.v0i0.402

Abstract

Estrogen has anti-oxidative and anti-inflammatory properties, but its levels decrease in postmenopausal women who can trigger oxidative stress. One of the most damaging effects of ROS is lipid peroxidation, and the end product is Malondialdehyde (MDA). Similarly, aging endothelium has increased oxidative stress and endothelial cell sensitivity to apoptosis. This study aimed to determine the effect of cowpea extract on serum MDA levels, aortic endothelial cell counts, and brain MDA levels in the ovariectomy model. Cowpea extract can be used as an alternative to prevent and overcome the effects that occur during menopause, such as cardiovascular problems, decreased bone mineral density, and dementia. The study used 15-month-old female Rattus norvegicus, divided into six groups (OVX, SHAM, OVX+estradiol, OVX+Vu 1.25; 2.5; and 5 mg/kg BW/day). Serum and brain MDA levels were examined by ELISA method, while the number of aortic endothelial cells were examined on histopathological preparations with Hematoxylin & Eosin (HE) staining. The mean value of serum and brain MDA levels decreased with an increase in the dose given (p-value 0.016). The mean value of aortic endothelial cells between the dose groups did not significantly differ. However, the mean value showed an increasing trend as the dose of cowpea extract was given. The results of this study indicate that the extract of cowpea has the potential as an antioxidant to reduce serum and brain MDA levels, prevent a decrease in the number of aortic endothelial cells. As prevention, cowpea extract can be used as an antioxidant and consumed since premenopause to minimize problems that occur during postmenopause.
PENGARUH EKSTRAK MASERASI TEH HIJAU TERHADAP APOPTOSIS SEL GRANULOSA FOLIKEL OVARIUM PADA RATTUS NORVEGICUS YANG DIPAJAN MSG Endah Wijayanti; Tatit Nurseta; Eviana Norahmawati
MMJ (Mahakam Midwifery Journal) Vol 4 No 1 (2019): Volume 4 No. 1 Mei 2019
Publisher : Politeknik Kesehatan Kementerian Kesehatan Kalimantan Timur

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (510.154 KB) | DOI: 10.35963/midwifery.v4i1.121

Abstract

Abstract Monosodium glutamate is one of the additives used a food flavoring and is widely known by society. The consumption of MSG in large quantities leads to the lesions of the hypothalamus nucleus, leading to some changes in the reproductive system. The aim of this study is to find out the influence of green tea extract against Apoptosis cells Granulose ovarian follicle Rattus norvegicus Dipajan MSG. The research method of using a true experimental research approach post test only control group Design. The sample used was a female white rat (Rattus norvegicus) the number of 25 tails divided into 5 groups. Exposure of monosodium glutamate administered at a dose of 0.7 mg/grBB per oral and green tea extract a variety of doses administered 2 hours thereafter during 30 days after the rats were obtained in the Proestrus phase. Apoptosis examination uses In Situ Cell Death Detection Kit, POD catalog number 11684817910 of Roche brand with Tunnel method. Data were analyzed using the One Way ANOVA test. Results showed that the lowest average apoptosis rate was seen in the MSG Administration treatment group and green tea extract dose 1.4 mg/tail/day (18,686 ± 2,247) but increased again on the group's MSG administration treatment and green tea extract dose 2.8 mg/tail/day (23,752 ± 3,206). So the administration of green tea extract of varying doses can decrease apoptosis in the Rattus norvegicus that is Dipajan MSG. Keywords: green tea extract, monosodium glutamat, Apoptosis Abstrak Monosodium glutamat, merupakan salah satu zat aditif yang digunakan sebagai penyedap makanan dan dikenal secara luas oleh masyarakat. Konsumsi MSG dalam jumlah besar menyebabkan lesi bagian nucleus arkuata hipotalamus sehingga menimbulkan beberapa perubahan pada sistem reproduksi. Tujuan dalam penelitian ini untuk mengetahui pengaruh ekstrak teh hijau terhadap Apoptosis Sel Granulosa Folikel Ovarium Rattus norvegicus yang dipajan MSG. Metode Penelitian menggunakan penelitian true eksperimental pendekatan post test only control group design. Sampel yang digunakan adalah tikus putih betina (Rattus norvegicus) sejumlah 25 ekor yang terbagi menjadi 5 kelompok. Paparan monosodium glutamat diberikan dengan dosis 0,7 mg/grBB per oral dan ekstrak teh hijau berbagai dosis diberikan 2 jam setelahnya selama 30 hari setelah didapatkan tikus berada pada fase proestrus. Pemeriksaan Apoptosis menggunakan In Situ Cell Death Detection Kit, POD nomor katalog 11684817910 merk Roche dengan metode Tunel. Data dianalisis menggunakan uji One Way Anova. Hasil penelitian menunjukkan bahwa Nilai rerata apoptosis terendah tampak pada kelompok perlakuan pemberian MSG dan ekstrak teh hijau dosis 1,4 mg/ekor/hari (18.686± 2.247) namun meningkat lagi pada kelompok perlakuan pemberian MSG dan ekstrak teh hijau dosis 2,8 mg/ekor/hari (23.752± 3.206). Sehingga pemberian ekstrak teh hijau berbagai dosis dapat menurunkan apoptosis pada Rattus norvegicus yang dipajan MSG Kata Kunci : ekstrak teh hijau, monosodium glutamat, dan Apoptosis
Effect of Red Bean Extract (Phaseolus vulgaris L.) on IL-10 Levels in Systemic Lupus Erythematosus Model Mukhamad Nooryanto; Tatit Nurseta; Cholid Rohman Riskianto; Syah Sembung Wasiso; Yudistira Yunus
Asian Journal of Health Research Vol. 2 No. 2 (2023): Volume 2 No 2 (August) 2023
Publisher : Ikatan Dokter Indonesia Wilayah Jawa Timur

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55561/ajhr.v2i2.96

Abstract

Introduction: Systemic Lupus Erythematosus (SLE) is a is a chronic autoimmune inflammatory disease of unknown etiologic. Multiorgan dysfunction in women with SLE causes menstrual irregularities, ovarian failure, follicular atresia, and premature ovarian failure (POF) leading to the cessation of menstruation. Phaseolus vulgaris L. contains isoflavones that can trigger oocyte maturation, stimulate the formation of antral follicles, increase FSH receptor expression and proliferate cell nuclear antigen (PCNA) expression. This study aims to prove the effect of red bean extract (isoflavones) in SLE by and increasing the secretion of anti-inflammatory IL-10 levels. Material and Methods: This study used a true experimental design with a post-test-only controlled group design. The samples used in this study were at least 25 female Balb/c mice. Data on serum IL-10 levels in mice were obtained by measurement using ELISA. The data were statistically analysed using SPSS 16 for Windows software program. The data were tested for normality and homogeneity using parametric non-parametric analysis. Results: The results showed that there was a significant difference in ANA levels with a p-value of 0.000 in each sample. From this test, it was proven that there was a significant increase in IL-10 levels at doses of 75 mg/KgBW (P2) and 100 mg/KgBW (P3) (p-value=0.000) due to the administration of various doses of red bean extract (isoflavones). Conclusion: Red bean extract (isoflavones) had a significant effect in increasing IL-10 levels.
Negative Effect of Cigarette Smoke: Black Garlic Opportunities for Prevention of Ovulation Disorders Asih, Sari Wulan; Nurseta, Tatit; Handono, Kusworini; Yudhanto, Hendy Setyo
Medical Laboratory Technology Journal Vol. 10 No. 1 (2024): June
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31964/mltj.v10i1.585

Abstract

Women's issues are primarily related to infertility. This study aimed to demonstrate that ovarian healing in wistar rats exposed to cigarette smoke was impacted by the administration of extracted black garlic. This research uses True Experimental methods in vivo in the laboratory to identify the cause and effect of the variables being tested. This method involves replication, randomization, and control. The design chosen was a post-test-only control group design, where the experimental group received treatment and the control group did not, without random selection. The results of the study showed that there was no effect due to exposure to cigarette smoke with an increase in cortisol and a decrease in the number of secondary follicles in the ovaries of female wistar rats as well as the administration of Black Garlic (Allium sativum) extract. Black garlic extract unaffected cortisol levels and the number of secondary follicles or repair of the ovaries due to exposure to cigarette smoke. Further research is needed in dosing black garlic extract or combining it with other ingredients to provide effective results.
Nutritional status, hemoglobin, and albumin levels in predicting platinum resistance in ovarian cancer at Dr. Saiful Anwar Hospital, Malang, Indonesia Nurseta, Tatit; Widowati, Ayu Rizky
Majalah Obstetri & Ginekologi Vol. 32 No. 3 (2024): December
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/mog.V32I32024.168-173

Abstract

HIGHLIGHTS Nutrition status, hemoglobin, and albumin levels are not predictors for platinum resistance. Hemoglobin and albumin levels increased in both platinum-sensitive or platinum-resistant.   ABSTRACT Objective: This study aimed to determine whether nutritional status, hemoglobin, and albumin levels could serve as reliable predictors for predicting platinum resistance in patients with ovarian cancer. Materials and Methods: Conducted as a cross-sectional analysis, this study included 80 ovarian cancer patients who had completed six cycles of platinum-based chemotherapy. Patients were divided into two categories: those with platinum-resistant cancer and those with platinum-sensitive cancer, based on recurrence status following chemotherapy. Nutritional status was assessed through body mass index (BMI), and both hemoglobin and albumin levels were measured pre- and post-chemotherapy to investigate potential differences between the groups. Results: The analysis revealed no significant difference in BMI between the platinum-sensitive and platinum-resistant groups (p = 0.743), suggesting that nutritional status, as measured by BMI, did not correlate with platinum resistance. Hemoglobin levels were similarly non-significant before (p = 0.072) and after chemotherapy (p = 0.055), indicating no clear association between hemoglobin levels and platinum response. However, hemoglobin levels showed significant increases post-chemotherapy in both the platinum-sensitive (p = 0.002) and platinum-resistant (p = 0.025) groups, though without affecting resistance outcomes. Pre-chemotherapy albumin levels did not significantly differ between the two groups (p = 0.218); but a significant post-chemotherapy difference was observed (p = 0.027), with both groups experiencing substantial increases from pre- to post-chemotherapy (p = 0.000). Conclusion: The findings suggest that BMI, hemoglobin, and albumin levels are not reliable predictors of platinum resistance in ovarian cancer patients. Although both hemoglobin and albumin increased significantly after chemotherapy, these changes did not correspond with platinum resistance status.
Comprehensive Analysis of Artery Ligation Intervention in Placenta Percreta Management: A Systematic Review Nurseta, Tatit; Leksono, Setio
Asian Journal of Health Research Vol. 4 No. 1 (2025): Volume 4 No 1 (April) 2025
Publisher : Ikatan Dokter Indonesia Wilayah Jawa Timur

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55561/ajhr.v4i1.226

Abstract

Introduction: The artery ligation technique has been adopted as a life-saving strategy to minimize bleeding in obstetrical and pelvic procedures. However, there are only a few studies on the use of arterial ligation in surgeries for abnormally invasive placentation (AIP). The purpose of this systematic study is to assess how well arterial ligation in managing blood loss during placenta percreta surgery and assess the associated complications and adverse events.    Material and Methods: Systematic Reviews and Meta-Analyses Preferred Reporting Items (PRISMA) framework was used in this study. Studies on arterial ligation techniques in placenta percreta surgery were included based on criteria focusing on estimated blood loss, operative time, and adverse events. A comprehensive literature search across multiple databases was performed, resulting in three studies that met the inclusion criteria.    Results: The three studies included in the review with a total of 193 patients. The findings show that IIAL significantly decreased the projected amount of blood loss compared to control groups. In one study, bilateral IIAL showed lower blood loss (1076 ± 545 ml) compared to other interventions, while another study demonstrated that IIAL resulted in lower estimated blood loss compared to control groups. However, hypogastric artery ligation (HAL) was associated with higher blood loss in one study. Despite its effectiveness in controlling blood loss, complications such as common iliac artery thrombosis, pseudoaneurysms, and limb ischemia were reported, though with low incidence.    Conclusion: Arterial ligation, particularly IIAL, could be a viable strategy for managing blood loss during placenta percreta surgery.
The Relationship Between Physical Activity 7 Days Before Delivery And The Duration Of Active Phase Of First Stage Labor In Primipara Mothers Jundyah, Nazhifa Zalfa; Indahwati, Lilik; Rahayu, Indriati Dwi; Nurseta, Tatit
JKM (Jurnal Kebidanan Malahayati) Vol 11, No 4 (2025): Volume 11 No 4, April 2025
Publisher : Program Studi Kebidanan Fakultas Ilmu Kesehatan Universitas Malahayati

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33024/jkm.v11i4.19867

Abstract

Latar belakang: Persalinan yang berlangsung terlalu lama dapat meningkatkan risiko komplikasi bagi ibu dan janin, namun risiko tersebut dapat diminimalkan melalui aktivitas fisik yang dapat mempengaruhi kemampuan aerobik dan kardiorespirasi tubuh, yang mengakibatkan peningkatan maksimal asupan oksigen maksimum yang selanjutnya dapat meningkatkan pasokan energi. Pasokan energi ini penting untuk mendukung kontraksi otot miometrium selama persalinan, terutama pada tahap awal. Tujuan: Penelitian ini bertujuan untuk mengetahui hubungan aktivitas fisik dengan durasi persalinan kala I fase aktif pada ibu primipara. Metode: Penelitian menggunakan jenis penelitian observasional dengan pendekatan cross-sectional pada 30 ibu primipara di fasilitas kesehatan Malang. Hasil: Pengambilan data dilakukan dengan pengisian kuesioner PPAQ (Pregnancy Physical Activity Questionnaire) dan dianalisis menggunakan uji rank spearman. Hasil uji analisis diperoleh p = 0,049 (p < 0,05) yang berarti ada hubungan aktivitas fisik 7 hari sebelum persalinan terhadap durasi persalinan kala I fase aktif pada ibu primipara di Fasilitas Kesehatan Malang. Kesimpulan: Kesimpulan yang didapat yaitu semakin tinggi kategori aktivitas fisik 7 hari sebelum persalinan, maka durasi kecepatan persalinan kala I fase aktif semakin meningkat. Kata kunci: Aktifitas fisik, durasi persalinan kala I fase aktif, primipara. ABSTRACT Background: Prolonged labor can increase the risk of complications for the mother and fetus, but these risks can be minimized through physical activity that can affect the body's aerobic and cardiorespiratory capabilities, resulting in an increase in maximum maximum oxygen intake which can further increase energy supply. This energy supply is important to support myometrial muscle contractions during labor, especially in the early stages. Objective: This study aims to determine the relationship between physical activity and the duration of labor in active phase I in primiparous mothers. Methods: The study used observational research with a cross-sectional approach on 30 primiparous mothers in Malang health facilities. Results: Data were collected by filling out the PPAQ (Pregnancy Physical Activity Questionnaire) questionnaire and analyzed using the spearman rank test. The results of the analysis test obtained p = 0.049 (p < 0.05) which means that there is a relationship between physical activity 7 days before delivery on the duration of labor in active phase I in primiparous mothers in Malang Health Facility. Conclusion: The conclusion obtained is that the higher the category of physical activity 7 days before labor, the duration of the active phase I labor speed increases. Keywords: Physical activity, duration of active phase I labor, primipara.  
Vitamin D and its Role in Cell Cycle Dynamics: A Review of the Molecular and Clinical Studies Sastradinata, Irawan; Murti, Krisna; Saleh, Irsan; Nurseta, Tatit
Journal La Medihealtico Vol. 6 No. 3 (2025): Journal La Medihealtico
Publisher : Newinera Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37899/journallamedihealtico.v6i3.2096

Abstract

Vitamin D is an essential nutrient that not only plays a role in calcium homeostasis and bone health, but also has broad functions in the regulation of various biological processes, including cell cycle dynamics. This study aims to examine in depth the role of vitamin D in cell cycle dynamics based on evidence from molecular and clinical studies. The method used was qualitative research with a literature study approach, where data was collected from relevant articles such as PubMed, EuropePMC, and Google Scholar. Analysis was carried out through the process of filtering, presenting data, and drawing conclusions. The results showed that vitamin D in its active form, 1,25-dihydroxyvitamin D3, interacts with the vitamin D receptor (VDR) in cells and modulates the expression of genes involved in cell proliferation. This mechanism involves an increase in the expression of cell cycle inhibitory genes, such as p21 and p27, and a decrease in the expression of cell cycle promoting genes, such as cyclin D1, which contributes to the arrest effect on the cell cycle. Thus, in addition to being important for bone health, vitamin D has a significant role in cell cycle regulation, and its deficiency can increase the risk of various diseases, including cancer. Therefore, maintaining optimal vitamin D levels through sun exposure, consumption of foods rich in vitamin D, or supplementation is essential. Further research is needed to understand the role of vitamin D in genetic regulation, particularly in relation to cancer.
Correlation of Leucine Intake with Serum Prolactin and Breast Milk Protein Levels in Breastfeeding Women Lestari, Ni Putu Yunita Sri; Wardani, Widya Kusuma; Ratnawati, Linda; Nurseta, Tatit; Rahardjo, Bambang
Devotion : Journal of Research and Community Service Vol. 3 No. 10 (2022): Devotion: Journal of Research and Community Service
Publisher : Green Publisher Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36418/dev.v3i10.199

Abstract

Breast milk is the best choice food for a baby’s growth and development of infants at some point of the primary 6 months of life. Fulfillment of nutrition during breastfeeding is an important requirement, one of which is the intake of the amino acid leucine which is said to affect prolactin as a regulator of breast milk production and protein synthesis in breast milk. This can support the quality and quantity of breast milk to meet the nutritional needs of infants support infant growth and development and reduce the incidence of stunting at the age of toddlers. This research aims to determine the relationship between leucine intake and levels of prolactin and protein in breast milk in breastfeeding mothers. This research is an analytic observational study with a cross-sectional method. This study carried out normality using the Kolmogorov-Smirnov test and using the Pearson correlation test to determine the relationship between variables. Based on the test results of the relationship between leucine intake and serum prolactin levels, a significance value of 0.005 (p<0.05) was obtained with a correlation coefficient value of 0.183 and the relationship between leucine intake and breast milk protein levels obtained a significance value of 0.033 (p<0.05) with a correlation coefficient of 0.147. The results of this test showed a significant relationship between these variables. There is a weak correlation between leucine intake and levels of prolactin and protein in breast milk
Effect of Bengkoang (Pachyrhizus erosus) Extract on Estrogen Receptor-?, Progesterone Receptor Expression, and Follicle-Stimulating Hormone Levels Suryanti; Putri Ardela, Mayasari; Frenty Hadiningsih, Eka; Winarsih, Sri; Noorhamdani; Nurseta, Tatit; Norahmawati, Eviana
Indonesian Journal of Obstetrics and Gynecology Volume 13. No. 3 July 2025
Publisher : Indonesian Socety of Obstetrics and Gynecology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32771/inajog.v13i3.2180

Abstract

Abstract Objective: Progesterone functions by inhibiting the release of Gonadotropin-Releasing Hormone (GnRH), which decreases Follicle-Stimulating Hormone (FSH) levels and converts them into hypoestrogens. This condition affects the expression of steroid, estrogen, and progesterone receptors, contributing to endometrial proliferation and secretion during the menstrual cycle. Methods: This study aims to demonstrate that Bengkoang extract administration increases the expression of Estrogen Receptor (ER), Progesterone Receptor (PR), and Follicle-Stimulating Hormone (FSH) in Wistar model rats. This study divided 25 female Wistar rats into five groups: one control group without progesterone and Bengkoang extract and four treatment groups injected with progesterone. After exposure, Bengkoang extract was administered to three treatment groups at doses of 70 mg/200 g BW/day (treatment 1), 140 mg/200 g BW/day (treatment 2), and 280 mg/200 g BW/day (treatment 3). Results: The results showed an increase in the expression of Estrogen Receptor- ? (Er?), Progesterone Receptor (PR), and Follicle-Stimulating Hormone (FSH) levels in treatment 1, treatment 2, and treatment 3 groups compared to the KP with p <0.05 Conclusion: The study investigating the effect of Bengkoang (Pachyrhizus erosus) extract on estrogen receptor-? (ER?), progesterone receptor (PR) expression, and follicle-stimulating hormone (FSH) levels has demonstrated significant findings. Keywords: Estrogen, Estrogen receptor-?, Follicle-stimulating hormone, Pachyrhizus erosus, Progesterone, Progesterone receptor
Co-Authors A.A. Ketut Agung Cahyawan W AA Sudharmawan, AA Adys Werestandina, Adys Agustina Tri Endharti Ambar Dwi Retnoningrum Anandasari , Noni Fidya Ayu Anandasari, Noni Fidya Ayu Angelica, Aina Anisak, Siti Asih, Sari Wulan Bambang Rahardjo Cholid Rohman Riskianto Diana Lyrawati Dwi Yuni Nur Hidayati Elisa Danik Kurniawati Endah Wijayanti Eviana Norahmawati Fajar Ari Nugroho Frenty Hadiningsih, Eka Hidayat Sujuti Husnul Khotimah Indahwati, Lilik Indriati Dwi Rahayu jundyah, nazhifa zalfa Juniartha, Putu Karyono Mintaroem Kaspirayanthi, Ni Ketut Devy Kenty Wantri Anita, Kenty Wantri Kridayanti, Novalia Krisna Murti Kusworini Handono Leksono, Setio Lestari, Ni Putu Yunita Sri Mudinillah, Adam Muhammad Irsan Saleh Muhammad Rasjad Indra Muhammad Rivai Mukhamad Nooryanto Natalina, Riny Ni Putu Sri Haryati Noorhamdani Novida Ariani Permata, Dear Ayu Prawiro, Sumarno Reto Putri Ardela, Mayasari Rahajeng Rahajeng Rahmi Utami Ratnawati, Putu A.R. Sastradinata, Irawan Selvi Marcellia Sitompul, Barlian Sri Winarsih Subandi Subandi SURYANTI Syah Sembung Wasiso Tanti Tri Lestary Tri Yudani MR Wardani, Widya Kusuma Wati, Linda Ratna Widowati, Ayu Rizky Wijasa, Arsana Wiyasa, I W.A. Wiyasa, I Wayan Arsana Yahya Irwanto Yudhanto, Hendy Setyo Yudistira Yunus