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Induksi Kalus Sorgum Sorgum (Sorghum bicolor L.) Pada Kombinasi 2,4-D dan BAP Secara In Vitro Dwi Yanti, Faradillah Febri; Wardana, Rudi
Jurnal Javanica Vol. 2 No. 2 (2023): Jurnal Terapan Agribisnis
Publisher : Politeknik Negeri Banyuwangi Jl. Raya Jember km. 13 Labanasem, Kabat, Banyuwangi, Jawa Timur (68461) Telp. (0333) 636780

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.57203/javanica.v2i2.2023.72-80

Abstract

Sorgum merupakan tanaman pangan jenis serealia yang berpotensi untuk dikembangkan. Namun, terdapat permasalahan dalam keragaman genetik dan kurang tersedianya varietas unggul membuat proses pengembangannya menjadi terhambat. Penelitian ini bertujuan untuk mengetahui hasil induksi kalus tanaman sorgum pada pemberian kombinasi zat pengatur tumbuh 2,4-D dan BAP. Percobaan dilakukan menggunakan Rancangan Acak Lengkap Faktorial dengan faktor pertama yaitu konsentrasi 2,4-D yang terdiri dari 1 mg/L (A1) dan 2 mg/L (A2), sedangkan faktor kedua yaitu konsentrasi BAP yang terdiri dari 1 mg/L (B1), 1,5 mg/L (B2), 2 mg/L (B3), dan 2,5 mg/L (B4). Hasil penelitian menunjukkan bahwa kombinasi perlakuan 2 mg/L 2,4-D + 1,5 mg/L BAP memberikan hasil terbaik pada kedinian muncul kalus (6 HSI) dan kualitas kalus. Sedangkan pada variabel diameter kalus, kombinasi 2 mg/L 2,4-D + 2,5 mg/L BAP menjadi perlakuan terbaik dengan diameter kalus sebesar 6,33 cm.
Growth and yield of maize applicated by Rizhobium spp. from legume and non-legume rhizosphere Widodo, Tirto Wahyu; Muhklisin, Ilham; Nugroho, Setyo Andi; Wardana, Rudi; Ummah, Umi Safitri Alifia
Journal of Agriculture and Applied Biology Vol 4 No 2 (2023): Journal of Agriculture and Applied Biology
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jaab.04.02.05

Abstract

Rhizobium spp can affect plant growth in non-legume crops directly by synthesis of phytostimulator and solubilization of inorganic phosphate. However, the ability of Rhizobium spp from legume and non-legume rhizosphere to trigger the growth of non-legume crops is different. This research aims to analyze the ability of Rhizobium spp isolates to produce IAA hormones and phosphate solvent and determine the response of maize with the application of Rhizobum spp from the legume and non-legume rhizosphere. This study was carried out in two stages, where the first stage at Bioscience Laboratory Politeknik Negeri Jember, while the second stage at Kaliurang field, Jember, Indonesia (altitude 146 m asl, temperature 21°C - 34°C, and soil type was incepstisol) from September 2022 to January 2023. The field experiment was arranged in a complete randomized design (crd) with the application of Rhizobium spp isolates from various rhizosphere as a treatment consisting of without Rhizobium spp (control), maize-rhizosphere isolate, rice-rhizosphere isolate, soybean-rhizosphere isolate, edamame-rhizosphere isolate, and peanut-rhizosphere isolate. Every treatment was replicated four times. The results showed that Rhizobium spp isolates from legume and non-legume rhizospheres can synthesize indole acetic acid and solubilize phosphate. This condition was indicated by the solubilized phosphate content in the planting medium, which was higher in the application of Rhizobium spp compared to the control. Inoculation of Rhizobium spp from several rhizospheres showed a significant effect on plant height, stem diameter, ear weight without husks, and ear dry weight compared to control. These bacteria are able to trigger the growth of maize through direct and indirect mechanisms. In addition, the plant height that was treated with maize-rhizosphere Rhizobium spp was better than rice. It is suspected that Rhizobium spp from the maize rhizosphere is more adaptable when applied to growing media for maize crops, so that it can increase plant height.
Growth and yield of maize applicated by Rizhobium spp. from legume and non-legume rhizosphere Widodo, Tirto Wahyu; Muhklisin, Ilham; Nugroho, Setyo Andi; Wardana, Rudi; Ummah, Umi Safitri Alifia
Journal of Agriculture and Applied Biology Vol 4 No 2 (2023): Journal of Agriculture and Applied Biology
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jaab.04.02.05

Abstract

Rhizobium spp can affect plant growth in non-legume crops directly by synthesis of phytostimulator and solubilization of inorganic phosphate. However, the ability of Rhizobium spp from legume and non-legume rhizosphere to trigger the growth of non-legume crops is different. This research aims to analyze the ability of Rhizobium spp isolates to produce IAA hormones and phosphate solvent and determine the response of maize with the application of Rhizobum spp from the legume and non-legume rhizosphere. This study was carried out in two stages, where the first stage at Bioscience Laboratory Politeknik Negeri Jember, while the second stage at Kaliurang field, Jember, Indonesia (altitude 146 m asl, temperature 21°C - 34°C, and soil type was incepstisol) from September 2022 to January 2023. The field experiment was arranged in a complete randomized design (crd) with the application of Rhizobium spp isolates from various rhizosphere as a treatment consisting of without Rhizobium spp (control), maize-rhizosphere isolate, rice-rhizosphere isolate, soybean-rhizosphere isolate, edamame-rhizosphere isolate, and peanut-rhizosphere isolate. Every treatment was replicated four times. The results showed that Rhizobium spp isolates from legume and non-legume rhizospheres can synthesize indole acetic acid and solubilize phosphate. This condition was indicated by the solubilized phosphate content in the planting medium, which was higher in the application of Rhizobium spp compared to the control. Inoculation of Rhizobium spp from several rhizospheres showed a significant effect on plant height, stem diameter, ear weight without husks, and ear dry weight compared to control. These bacteria are able to trigger the growth of maize through direct and indirect mechanisms. In addition, the plant height that was treated with maize-rhizosphere Rhizobium spp was better than rice. It is suspected that Rhizobium spp from the maize rhizosphere is more adaptable when applied to growing media for maize crops, so that it can increase plant height.
Pemanfaatan Keong Mas Untuk Pupuk Organik Cair di Kelompok Tani Podo Tentrem Kecamatan Wuluhan Jember Suharjono, Suharjono; Asmono, Sepdian Luri; Wardana, Rudi
Journal of Community Development Vol. 3 No. 3 (2023): April
Publisher : Indonesian Journal Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47134/comdev.v3i3.108

Abstract

The golden snail is one of the pests that damage rice plants, this pest has the ability to reproduce quickly, causing the golden snail to become one of the main pests in lowland rice. But the golden snail can be used as a basic material for liquid organic fertilizer (LOF) because it contains protein, Azotobacter, Azospirillum, phosphate solubilizing microbes, Pseudomonas, Staphylococcus, enzymes and the hormone auxin. The effectiveness of the golden snail LOF has been tested on several agricultural crops, one of which is rice. In the Podo Tentrem Farmers Group, these pests are simply thrown away and have not been utilized properly. Whereas the potential of this golden snail can be very high if it is processed into organic fertilizer. Therefore, with this community service activity, it is hoped that it can improve competence, skills and increase the production output of the Podo Tentrem farmer group. This can be measured by the enthusiasm of the participants who were attended by 100% of the invitees, besides that the participants also knew the stages of work and succeeded in making Keong Mas LOF well. In addition, other assessment indicators are using quantitative data on pre-test and post-test scores. Before the counseling was carried out, the pretest value was 50 and after the knowledge training, it increased with an average post-test score of 76. These results can indicate that in theory and practice, on average, participants understand and can make golden snail LOF.
Sosialisasi Pengaplikasi Centrifugal Force Dalam Memproduksi Minyak Kelapa Murni Wardana, Rudi; Salim, Abdurrahman; Nuraisyah, Anni; Nugroho, Setyo Andi
Journal of Community Development Vol. 3 No. 3 (2023): April
Publisher : Indonesian Journal Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47134/comdev.v3i3.110

Abstract

Cooking oil is one of the staple ingredients that is widely used in household and industrial needs. The high price of cooking oil has an impact on the food security of a family. Repeated use of cooking oil will also have an impact on family health. So it is necessary to socialize the healthy and quality cooking oil using virgin coconut oil. The purpose of this socialization was directed to the group of housewives in Pumo Village, Wuluhan District, Jember Regency. The economic condition of this housewife is classified as lower middle class. The Pumo Village Housewives Group (partner) is located in a coastal area with extraordinary coconut potential. Utilization of coconut is only limited to being sold in the form of coconuts, either young (degan) or old (copra). Though the potential will be greater if the processing is carried out, especially in the form of pure coconut oil. The solution offered to this problem is the application of science and technology with simple technology, namely centrifugal force as an effort to become independent partners in producing virgin coconut oil. The virgin coconut oil produced is not only to support family food security, but also to be a business opportunity for partners. The results of this service, the community can know the techniques of making, packaging products and entrepreneurship in the field of making virgin coconut oil.
Multiplikasi Tunas Kentang Merah (Solanum Tuberosum L.) dengan Aplikasi ZPT NAA Dan BAP Secara In Vitro Wardana, Rudi; Mauidah, Ana Uzunul; Jumiatun, Jumiatun; Widodo, Tirto Wahyu; Firgiyanto, Refa
Vegetalika Vol 13, No 4 (2024): In Publish
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/veg.95287

Abstract

Rendahnya produksi kentang merah di Indonesia dikarenakan terbatasnya ketersediaan bibit unggul dan bibit rentan terhadap serangan patogen. Perlu adanya solusi alternatif untuk meningkatkan mutu bibit kentang merah yang seragam dan bebas patogen yaitu melalui teknik kultur in vitro. Penelitian ini bertujuan untuk menganalisis konsentrasi terbaik NAA dan BAP terhadap multipikasi tunas kentang merah secara in vitro. Penelitian ini dilakukan di Laboratorium Kultur Jaringan Politeknik Negeri Jember. Rancangan percobaan menggunakan metode Rancangan Acak Lengkap Faktorial (RALF) yang terdiri dari atas dua faktor. Faktor pertama adalah konsentrasi Napthalene Acetic Acid (NAA) meliputi 0,1 mg/l; 0,3 mg/l; 0,5 mg/l. Faktor kedua adalah konsentrasi 6-Benzyl Amino Purine (BAP) meliputi 1 mg/l; 1,5 mg/l; 2 mg/l). Hasil penelitian menunjukkan bahwa aplikasi kombinasi konsentrasi 0,3 mg/l NAA dan 1,5 mg/l BAP berpengaruh berbeda nyata terhadap tinggi tunas (7,03 cm). Aplikasi konsentrasi NAA 0,1 mg/l berpengaruh berbeda nyata terhadap waktu munculnya kalus (9,44 HST). Pada  aplikasi konsentrasi BAP 1,5 mg/l berpengaruh berbeda nyata terhadap waktu muncul tunas yaitu 8,78 HST dan jumlah daun (7,58 helai).
PERTUMBUHAN DAN HASIL TANAMAN KEDELAI TERHADAP KOMBINASI PUPUK ANORGANIK DAN POC KEONG MAS (Glycine max L.) Wardana, Rudi; Arij Nabila, Dini; Wahyu Widodo, Tirto; Dwi Soelaksini, Liliek
Jurnal Javanica Vol. 3 No. 2 (2024): Jurnal Terapan Agribisnis
Publisher : Politeknik Negeri Banyuwangi Jl. Raya Jember km. 13 Labanasem, Kabat, Banyuwangi, Jawa Timur (68461) Telp. (0333) 636780

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.57203/javanica.v3i2.24.77-86

Abstract

Produksi kedelai di Indonesia hingga saat ini belum mampu mencukupi kebutuhan masyarakat Indonesia. Rerata kebutuhan kedelai secara nasional mencapai 3,4-3,6 juta ton pertahun, sedangkan produksinya hanya 20%-30% dari kebutuhan tersebut. Berdasarkan hal tersebut perlu adanya upaya untuk meningkatkan produksi tanaman kedelai yaitu dengan cara pemberian POC keong mas. Penelitian ini bertujuan untuk mengkaji pengaruh pemberian kombinasi pupuk anorganik dan POC keong mas terhadap pertumbuhan dan hasil tanaman kedelai. Penelitian ini menggunakan Rancangan Acak Kelompok (RAK) Non faktorial yang terdiri atas 7 perlakuan yaitu tanpa POC (100% pupuk anorganik), konsentrasi POC 250 ml/l +50% anorganik, 300 ml/l+50% anorganik, 350 ml/l +50% anorganik, 400 ml/l +50% anorganik, 450 ml/l+50% anorganik dan 500 ml /l+50% anorganik. Sebanyak 4 kali ulangan. Hasil penelitian menunjukkan bahwa konsentrasi POC 500 ml/l+50% anorganik menunjukkan hasil tertinggi pada tinggi tanaman, jumah cabang produktif, berat biomassa tanaman kedelai, jumlah polong persampel, berat segar polong persampel, berat kering polong persampel dan berat kering biji persampel.
PENGARUH KOMBINASI PELEPAH DAN TEMPURUNG KELAPA SEBAGAI INSEKTISIDA ASAP CAIR TERHADAP SERANGAN WALANG SANGIT (Leptocorisa Oratorius F.) Istiqomah, Ayunia; Wardana, Rudi
Jurnal AGROHITA: Jurnal Agroteknologi Fakultas Pertanian Universitas Muhammadiyah Tapanuli Selatan Vol 9, No 4 (2024): Jurnal Agrohita
Publisher : Fakultas Pertanian Universitas Muhammadiyah Tapanuli Selatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31604/jap.v9i4.11694

Abstract

Serangan hama merupakan salah satu kendala yang dapat menurunkan produktivitas tanaman. Pada budidaya padi, walang sangit merupakan hama yang menyerang ketika bulir berada pada fase masak susu. Penggunaan insektisida sintetik yang memiliki tingkat racun tinggi, namun berdampak kurang baik bagi tanaman sehingga diperlukan adanya insektisida nabati yang lebih ramah lingkungan. Penelitian ini bertujuan untuk mengetahui pengaruh kombinasi pelepah dan tempurung kelapa sebagai insektisida asap cair terhadap serangan walang sangit pada tanaman padi. Percobaan dilakukan menggunakan uji laboratorium dan uji lapang. Uji laboratorium dilakukan menggunakan rancangan acak lengkap (RAL) yang terdiri dari 6 perlakuan yaitu P0 : kontrol (aquades), P1 : konsentrasi 7 mL/100 mL air, P2 : konsentrasi 12 mL/100 mL air, P3 : konsentrasi 17 mL/100 mL air, P4 : konsentrasi 22 mL/100 mL air, dan P5 : konsentrasi 27 mL/100 mL air. Adapun percobaan di lapang dilakukan dengan membandingkan penggunaan insektisida nabati dan sintetik. Hasil menunjukkan bahwa insektisida asap cair kombinasi pelepah dan tempurung kelapa memiliki tingkat toksisitas LC50 sebesar 12% dan LC95 sebesar 47% dengan hasil yang tidak signifikan dengan penggunaan insektisida sintetik pada semua parameter pengamatan.
Respon Hasil Produksi Kacang Tanah (Arachis hypogea) terhadap Aplikasi PGPR dan Rhizobium Wardana, Rudi; Prayudi, Angga; Erdiansyah, Iqbal
Jurnal Ilmiah Inovasi Vol 25 No 1 (2025): April
Publisher : Politeknik Negeri Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Increasing peanut production can be done by applying rhizobium and PGPR. This study aims to examine the effect of PGPR and Rhizobium on peanuts. The research was conducted from December 2022 to February 2023 in Patemon Village, Pakusari District, Jember Regency. Experimental design using factorial RAK. The first factor was the concentration of PGPR consisting of 10.5 ml/l, 12.5 ml/l and 14.5 ml/l. The second factor was the dose of Rhizobium inoculants consisting of 25 kg/ha, 50 kg/ha, and 75 kg/ha. The results of this study indicated that there was no real interaction between the PGPR and Rizobum treatments. As a single factor, PGPR with a concentration of 12.5 ml/l gave an effect on the variable number of ginophores (72.1). While the application of Rhizobium inoculant at a dose of 75 kg/ha showed the best results on the number of pods/sample (30.90), weight of pods/sample (62.12 g), weight of seeds/sample (44.74 g), and weight of seeds/plot (850.60 gr). . It is suspected that the application of PGPR to peanuts and inoculation of Rhizobium plays a role in increasing nutrient uptake which in turn increases growth and production.
Aplikasi Asam Amino Ikan Lemuru dan PGPR Akar Edamame Terhadap Laju Pengisian Polong: Application of Lemuru Fish Amino Acids And Edamame Root PGPR To Pod Filling Rate Jumiatun, Jumiatun; Rudi Wardana; Ilham Muhklisin; Anni Nuraisyah; Septa Dwi Angga
Jurnal Ilmiah Inovasi Vol 25 No 1 (2025): April
Publisher : Politeknik Negeri Jember

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Abstract

Pengisian polong edamame (Glycine max L.) merupakan fase penting dalam produksi polong premium. Penelitian ini bertujuan untuk menganalisis pengaruh aplikasi asam amino ikan lemuru dan Plant Growth-Promoting Rhizobacteria (PGPR) berbasis akar edamame, terhadap laju pengisian polong. Rancangan yang digunakan dalam penelitian adalah rancangan acak kelompok faktorial (RAKF) terdiri atas dua faktor yaitu asam amino dan PGPR, perlakuan asam amino terdiri dari (0,5,10,15, dan 20)ml/l. Dan aplikasi PGPR terdiri dari (0, dan 150)ml/l. Berdasarkan hasil penelitian perlakuan asam amino memberikan pengaruh berbeda nyata pada pengamatan jumlah polong total dan persentase polong hampa. Sedangkan perlakuan PGPR menunjukkan hasil berbeda nyata pada pengamatan persentase polong hampa. Interaksi pada perlakuan PGPR dan asam amino berbeda tidak nyata pada semua variabel pengamatan,