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ANALISIS PRODUKSI DAN DISTRIBUSI PEMBENIHAN IKAN KOI (Cyprinus carpio) BERDASARKAN SEBARAN KUALITAS SELEKSI DI OMAH KOI FARM INDONESIA Fauzan, Agung Lutfi; Budiardi, Tatag; Effendi, Irzal; Diatin, Iis; Hadiroseyani, Yani; Dewi, Nina Nurmalia
Berita Biologi Vol 23 No 1 (2024): Berita Biologi
Publisher : BRIN Publishing (Penerbit BRIN)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/beritabiologi.2024.905

Abstract

Koi fish (Cyprinus carpio) is a freshwater ornamental fish commodity that has important economic potential, both nationally and internationally. Breeding is an activity in cultivation to produce seeds which are very decisive at the next stage of cultivation activities. The purpose of this study was to evaluate the technique and distribution of hatchery based on the distribution of quality selection of koi fish at Omah Koi Farm Indonesia. The test parameters measured were fecundity, fertilization rate (FR), hatching rate (HR), seed survival, seed quality, and water quality. The hatchery process includes pond preparation, parent selection, spawning, hatching eggs, rearing larvae, harvesting larvae, stocking larvae, feeding, feeding seeds, harvesting seeds, selecting seeds, and managing water quality. The results of the Kohaku and Showa fecundity calculations were 30,000 and 35,000 items respectively. The FR value obtained is 90% and HR is 83%. The average survival of koi seeds aged 45 days was 81.67% with an average of 150 high quality (HQ) seeds, 450 grade A seeds, and 450 grade B seeds. The temperature range in the larval rearing ponds was 25-27 0C, the water pH ranged from 7.9-8.5, DO ranged from 5.0-6.0, and ammonia was 0.01.
Analisis Kualitas Perairan Berdasarkan Tingkat Kesuburan dan Status Pencemaran Air Pesisir Bomo, Banyuwangi, Indonesia: Analysis of Water Quality Based on Trophic Status and Pollution Status in Bomo Coastal Waters, Banyuwangi, Indonesia Dewi, Nina Nurmalia; Nindarwi, Daruti Dinda; Sari, Putri Desi Wulan; Fauzan, Agung Luthfi
JFMR (Journal of Fisheries and Marine Research) Vol. 9 No. 3 (2025): JFMR on November
Publisher : Faculty of Fisheries and Marine Science, Brawijaya University, Malang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jfmr.2025.009.03.4

Abstract

Perairan pesisir Bomo Banyuwangi merupakan wilayah pesisir yang potensial yang menjadi habitat bagi berbagai organisme akuatik. Adanya pengaruh antropogenik dapat berpotensi mempengaruhi kondisi perairan. Penelitian ini bertujuan untuk mengetahui kondisi lingkungan perairan berdasarkan penentuan tingkat kesuburan dan status pencemaran air pesisir Bomo. Penelitian dilaksanakan di wilayah pesisir Bomo dengan pengambilan sampel di tiga stasiun yang diambil berdasarkan jarak perairan dari daratan. Setiap stasiun diambil sampel sebanyak 3 kali ulangan yang diambil selama 2 bulan pengamatan. Pengambilan sampel meliputi parameter kualitas air yang terdiri dari parameter fisika, kimia, dan biologi yang dianalisis secara insitu maupun exsitu. Data kualitas air dianalisis tingkat kesuburannya menggunakan pendekatan Trophic State Index (TSI) dan Trophic Level Index (TLI). Status pencemaran air dianalisis berdasarkan indeks storet dan indeks pencemaran (pollution index). Berdasarkan hasil penelitian, didapatkan bahwa tingkat kesuburan perairan berada pada kategori eutrofik dengan nilai TSI 74,31-76,58 dan super-eutrofik dengan nilai TLI 6,39-6,84, sedangkan status pencemaran air dipesisir Bomo tergolong memiliki pencemaran yang tinggi dengan nilai indeks storet -37 dan -41 pada bulan Juli dan September dan berdasarkan indeks pencemaran (IP) memiliki nilai 9,10 dan 5,77 untuk masing-masing bulan Juli dan September. Hal ini menandakan bahwa adanya masukan limbah ke perairan yang mempengaruhi kondisi perairan pesisir Bomo.   Bomo Coastal Water Banyuwangi are a potential coastal area that is a habitat for various aquatic organisms. The presence of anthropogenic activity can potentially affect the condition of the waters. This study aims to determine the condition of the aquatic environment based on determining the level of trophic status and pollution status of the Bomo coastal waters. The study was conducted in the coastal area of ​​Bomo by taking samples at three stations taken based on the distance of the waters from the mainland. Each station was sampled 3 times during 2 months of observation. Sampling included water quality parameters consisting of physical, chemical, and biological parameters which were analyzed in situ and ex situ. Water quality data were analyzed for their trophic status using the Trophic State Index (TSI) and Trophic Level Index (TLI) approaches. Pollution status was analyzed based on the storet index and pollution index. Based on the results of the study, it was found that the level of water trophic status was in the eutrophic category with a TSI value of 74.31-76.58 and super-eutrophic with a TLI value of 6.39-6.84, while the status of water pollution on the Bomo coastal waters was classified as having high pollution with a Storet Index value of -37 and -41 in July and September and based on the Pollution Index had a value of 9.10 and 5.77 for July and September respectively. This indicates that there is waste input into the waters that affects the condition of the Bomo coastal waters.
Polyvalent formalin-killed Aeromonas hydrophila vaccine with oil adjuvant drives coordinated humoral, innate, and cytokine responses in giant gourami (Osphronemus goramy) Rozi; Wiwiek Tyasningsih; Jola Rahmahani; Eduardus Bimo Aksono; Muchammad Yunus; Mohammad Anam Al-Arif; Suryo Kuncorojakti; Daruti Dinda Nindarwi; Putri Desi Wulan Sari; Nina Nurmalia Dewi; Woro Hastuti Satyantini; Muhammad Browijoyo Santanumurti; Dita Wisudyawati; Mohammad Noor Amal Azmai; Annas Salleh; Gazali Salim; Suwarno, Suwarno
Jurnal Ilmiah Perikanan dan Kelautan 2026: JIPK VOLUME 18 ISSUE 1 YEAR 2026 (FEBRUARY 2026, ISSUE IN PROGRESS)
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Graphical Abstract  Highlight Research Adjuvanted polyvalent FKC elicited the strongest and most sustained multi-arm immune response in Osphronemus goramy compared with monovalent and non-adjuvanted vaccines. The lead formulation combined high agglutinating antibody titres with enhanced NBT respiratory burst, indicating synergistic humoral–innate activation against Aeromonas hydrophila. Polyvalent vaccines did not dilute immunogenicity; instead, strain combination plus adjuvant broadened and amplified immune responsiveness. Longitudinal profiling of il-1β and ifn-γ revealed a stable pro-inflammatory/Th1-like cytokine signature uniquely associated with the adjuvanted polyvalent FKC. The integrated immunological “fingerprint” supports the adjuvanted polyvalent FKC as a rational lead candidate for motile Aeromonas septicaemia control in warm-water gourami aquaculture.   Abstract Bacterial septicaemia caused by Aeromonas hydrophila is an important constraint for giant gourami (Osphronemus goramy) culture in Southeast Asia. Inactivated whole-cell (bacterin) vaccines are widely used against bacterial diseases in aquaculture, but comparative data on monovalent versus polyvalent A. hydrophila vaccines, with and without oil-based adjuvant, remain scarce for this species. This study evaluated the safety, immunological responses, and protective efficacy of three formalin-killed cell (FKC) vaccines prepared from gourami-derived A. hydrophila isolates: a monovalent FKC (P2), a non-adjuvanted polyvalent FKC (P3), and an oil-adjuvanted polyvalent FKC (P4), using PBS (P1) as a control. Sub-adult giant gourami were vaccinated intraperitoneally and monitored for 42 days. Serum agglutinating antibody titres, nitroblue tetrazolium (NBT)-reducing activity, and splenic il-1β and ifn-γ mRNA expression were measured at multiple time points. At 21 days post-vaccination, a separate cohort was challenged intraperitoneally with virulent A. hydrophila Ah-S1, and survival was recorded for 14 days; relative percent survival (RPS) was calculated at day 14. All FKC formulations were clinically well tolerated, with only transient post-vaccination inappetence and no gross injection-site pathology. Vaccination induced clear, treatment- and time-dependent increases in agglutinating antibody titres, NBT-reducing activity, and splenic il-1β and ifn-γ transcription, with the strongest and most sustained responses in P4, intermediate responses in P3 and P2, and minimal changes in P1. Following homologous challenge, day-14 survival was 8.3% in P1, 61.7% in P2, 75.0% in P3, and 83.3% in P4, with corresponding RPS values of 58.2%, 72.7%, and 81.8%, respectively. Under these experimental conditions, the oil-adjuvanted polyvalent FKC (P4) produced the greatest enhancement of immune responses and protection against intraperitoneal A. hydrophila challenge in giant gourami. These findings support this formulation as a candidate for further vaccine development in O. goramy and highlight the need for dose optimisation, safety assessment, heterologous challenge, and field-validation studies before recommendations for large-scale use in aquaculture.