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All Journal Jurnal Veteriner Media Kedokteran Hewan Jurnal Medik Veteriner Jurnal Layanan Masyarakat (Journal of Public Service) Ovozoa: Journal of Animal Reproduction journal of Basic Medical Veterinary

Hermin Ratnani

Department Of Veterinary Medicine, Faculty Of Veterinary Medicine, Airlangga University, Surabaya

Author-ID : 3488516

Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Environmental Science Immunology & microbiology Medicine & Pharmacology Social Sciences Veterinary Other

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Mulberry (Morus alba L.) leaf extract enhanced spermatozoa motility, viability, and plasma membrane integrity of rats (Rattus norvegicus) exposed to e-cigarette smoke Ramadhani, Fifi Fauziah; Yulianna Puspitasari; Hermin Ratnani; Budi Utomo; Rochmah Kurnijasanti; Kadek Rachmawati
Ovozoa: Journal of Animal Reproduction Vol. 13 No. 2 (2024): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v13i2.2024.120-128

This study investigates the impact of mulberry leaf extract on the viability, plasma membrane integrity, and motility of spermatozoa from male white rats (Rattus norvegicus) exposed to e-cigarette smoke. A total of twenty-five male rats were divided into five groups: negative control (NC), positive control (PC), T1, T2, and T3. All groups, except for the NC group, were exposed to e-cigarette smoke. Rats in the T1, T2, and T3 groups received mulberry leaf extract in doses of 100, 200, and 400 mg/kg bw, respectively, while the NC and PC groups were given a placebo of 1% Na-CMC. Both the mulberry leaf extract and the placebo were administered daily, beginning three days prior to the start of e-cigarette smoke exposure, which lasted for 28 days. Results showed that spermatozoa motility, plasma membrane integrity, and viability in the experimental groups were significantly lower than those in the NC group (p <0.05). Conversely, rats in the T1, T2, and T3 groups that received mulberry leaf extract demonstrated significantly greater spermatozoa viability, plasma membrane integrity, and motility compared to the PC group (p <0.05). The T3 group exhibited the most pronounced improvements, with significantly enhanced spermatozoa viability, membrane integrity, and motility (p <0.05) relative to the PC group. These results indicate that mulberry (Morus alba L.) leaf extract enhanced spermatozoa viability, plasma membrane integrity, and motility in white rats (Rattus norvegicus) subjected to e-cigarette smoke.

Estrus quality, non-return rate and conception rate in the incidence of repeat breeding in dairy cow after 2%povidone iodine therapy combined with prostaglandin F2α at KUD Tani Wilis Tulungagung Zahrorona, Amira Halimah; Sri Pantja Madyawati; Pudji Srianto; Hermin Ratnani; Sunaryo Hadi Warsito; Sri Hidanah
Ovozoa: Journal of Animal Reproduction Vol. 14 No. 1 (2025): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v14i1.2025.46-51

Repeat breeding is a condition in which cows fail to conceive after being bred three or more times. This study aims to determine the quality of estrus, non-return rate (NRR) and conception rates obtained after cows were treated with 2% povidone iodine. The study involved 30 Holstein Friesian cows, each having calved three times and experiencing repeat breeding. Cows in estrus were treated with 10 mL of 2% povidone iodine intrauterinely, followed by an intramuscular injection of 2 mL (250 mg/head) of prostaglandin F2α (PGF2α, Luteosyl) five days later. Estrus quality was observed by examining changes in vulvar mucosa color, mucus discharge, and vulvar temperature. Pregnancy diagnosis was made at 60 days. Results showed that 43.3% of cows had estrus quality score 3 (cows showed dark red vulvar mucosa, transparent mucus, temperature 38.9 ± 0.5 ºC), 23.3% had score 2 (cows showed reddish vulvar mucosa, some cloudy mucus, temperature 38.7 ± 1.0 ºC), and 33.3% had score 1 (cows showed pink vulvar mucosa, cloudy mucus, temperature 38.6 ± 0.5 ºC). Of the 30 cows, 20 had a non-return rate (NRR) of 66.7%. Based on these results, it can be concluded that 2% povidone iodine has a positive effect on estrus quality, NRR, and conception rates in dairy cows experiencing repeat breeding.

PENGARUH PENAMBAHAN EKSTRAK TEH HIJAU (Camellia sinensis) DALAM BAHAN PENGENCER KUNING TELUR SITRAT TERHADAP KUALITAS SPERMATOZOA DOMBA SAPUDI YANG DISIMPAN PADA SUHU DINGIN Khoirunnisa, Itrah; Susilowati, Suherni; Maslachah, Lilik; Suprayogi, Tri Wahyu; Kurnijasanti, Rochmah; Ratnani, Hermin
Ovozoa: Journal of Animal Reproduction Vol. 8 No. 2 (2019): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

The purpose of this research was determine the effect and the best concentration of greentea extract in egg yolk citrate diluent for quality Sapudi sheep spermatozoa were measured in motility, viability and intact plasma membrane that was stored on cold temperature. The semen was devided into four groups : egg yolk citrate diluent, 0,05 % green tea extract in egg yolk citrate diluent, 0,1 % green tea extract in egg yolk citrate diluent and 0,15 % green tea extract in egg yolk citrate diluent. Spermatozoa quality was observed day 1, day 2, day 3, day 4 and day 5 after diluent. The data obtained was analyzed with the analysis of variance (ANOVA), followed by multiple range test duncan. The result showed that the higest precentage of motility, viability and intact plasma membran were obtained from 0,05 % green tea extract in egg yolk citrate diluent for 1 day of storage which were 85.83 ± 3.76, 91.16 ± 2.13 and 66.83 ± 6.49. The lowest precentage of motility, viability and intact plasma membrane were obtained from egg yolk citrate diluent for 5 day of storage which were 26.66 ± 6.05, 49.16 ± 6.43 and 26.66 ± 4.32. Conclution of this research was the addition of 0,05 % green tea extract can be used as a diluent could maintain the precentage of spermatozoa quality until 5 days after diluent.

HUBUNGAN MORFOMETRI AMBING TERHADAP PRODUKSI SUSU KUDA DI DAERAH BIMA NUSA TENGGARA BARAT Mujahid, Mujahid; Mustofa, Imam; Tehupuring, Benjamin Chr; Restiadi, Tjuk Imam; Eliyani, Hana; Ratnani, Hermin
Ovozoa: Journal of Animal Reproduction Vol. 8 No. 2 (2019): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Objective from this research was to determine the relationship of udder morphometry which consists of the height of the front udder, the height of the rear udder and the circumference ofudder on milk production in the Bima District of West Nusa Tenggara. One of ways to choose a good lactational mare is to pay attention at the shape of the part external body which is udder. The sample had used 32 mares that were in lactation period. Milk production has been measured from the average milk production per day in the lactation period. Milking process in Bima District, West Nusa Tenggara has done in three to four times a day in the span of four to five hours per day. Body length had been measured from os thoracalis to os coxae, chest circumference had been measured from os vertebrae thoracalis to os thoracalis, posterior abdomen of pelvic to os pelvis and the last of os lumbar vetebrae. The research design in this study was survey on every wild mares rancher. Data that has been collected and processed using Multiple Regression with SPSS program version 23th. The result showed that there was a strong relationship between height of rear udder and udder circumference to milk production with back udder with height coefficient of 0.21 (P <0.05) and udder circumference was 0.11 (P <0.05) and equation in Y = 2.423 - 0.398LA + 0.793TAB. However, there is no significant relationship between the height of the front udder and milk production.

Effect of α-tocopherol supplementation in diluents on the motility, viability and plasma membrane integrity of Simmental bull spermatozoa after cooling Azura, Sarah; Ratnani, Hermin; Susilowati, Suherni; Hariadi, Mas'ud; Samik, Abdul; Soepranianondo, Koesnoto
Ovozoa: Journal of Animal Reproduction Vol. 9 No. 1 (2020): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Semen storage in cold temperatures might cause an increase in reactive oxygen species (ROS) production. This condition resulted in spermatozoa damage and quality decrease. This study was conducted to investigate the effects of α-tocopherol supplementation in diluents on the motility, viability, and plasma membrane integrity of Simmental bull spermatozoa after cooling. Semen samples were diluted in skim milk egg yolk supplemented with 0, 0.5, 1.0, and 1.5 mM α-tocopherol respectively for control, Tl, T2, and T3. Spermatozoa were evaluated for their motility, viability, and membrane integrity in cooling temperature (5°C). The daily evaluation showed that 1.5 mM α-tocopherol was the best in maintaining motility, viability, and plasma membrane integrity, while 1.0 mM α-tocopherol was only good for maintaining viability. Therefore, it can be concluded that α-tocopherol at the concentration of 1.5 mM was an efficient antioxidant supplement for Simmental cattle semen in skim milk egg yolk diluent.

Penggunaan kombinasi Gonadotropin untuk pengobatan hipofungsi ovarium pada sapi perah Masruro, Novia Alfi; Mulyati, Sri; Madyawati, Sri Pantja; Samik, Abdul; Ratnani, Hermin; Harijani, Nenny
Ovozoa: Journal of Animal Reproduction Vol. 9 No. 1 (2020): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

This case report aims to determine the response of estrus and pregnancy rates in dairy cows with ovarian hypofunction by administering a combination of gonadotropin hormones. The sample used was ten dairy cows that had been calving, were not pregnant, showed no signs of estrus for more than 60 days after the last calving and had ovarian hypofunction based on rectal palpation. All dairy cows were injected 300 IU PG-600, then divided into two groups, each injected with 100 and 300 IU hCG respectively for first group and second group, intramuscularly at the time of insemination. The results showed that the estrus response occurred in all cows, in 6-8 (6.8 ± 0.84) days (first group), and 6-7 (6.2 ± 0.45) days (second group). Trans-rectal palpation pregnancy diagnosis 60 days after artificial insemination showed that all dairy cows in both groups were pregnant. It could be concluded that the treatment of dairy cows suffering from ovarian hypofunction with a combination of 300 IU PG-600 and 100 IU hCG was sufficient to induce 100% estrus rates and 100% pregnancy rates.

Pengaruh injeksi nikotin terhadap motilitas, viabilitas, dan integritas membran spermatozoa mencit (Mus Musculus) Pramudita, Kautsar Pandu; Lokapirnasari, Widya Paramita; Susilowati, Suheni; Ratnani, Hermin; Safitri, Erma; Luqman, Epy Muhammad
Ovozoa: Journal of Animal Reproduction Vol. 9 No. 3 (2020): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v9i3.2020.77-81

The process of spermatogenesis is sensitive to nicotine, a main component of cigarette smoke. This study aimed to observe the effect of nicotine injection on motility, viability, and membrane integrity of the spermatozoa of mice (Mus musculus). The mice were 10 weeks old, weighing about 20 grams, and in healthy and fertile conditions. Mice were adapted for 1 week by being given feed in the form of pellets and drinking water every day. Twenty of the mice were divided into four groups. Group K as a control was injected with 0.9% NaCl at 2.5 mg/Kg bw. Group P1 was injected with nicotine at 2.5 mg/Kg bw. P2 was injected with nicotine at 5 mg/Kg bw. Group P3 was injected with nicotine at 10 mg/Kg bw. The injection was carried out subcutaneously as much as 0.5 ml once a day for 35 days. Data analysis used the Analysis of Variances (ANOVA), followed by Duncan's Multiple Range Test. The results showed that the viability, motility, and membrane integrity of the spermatozoa decreased significantly (p <0.05) according to the increase in the dose of nicotine injected. It could be concluded that subcutaneous nicotine injection linearly decreased the motility, viability, and membrane integrity of the spermatozoa of mice.

The effect of watermelon (citrullus lanatus) rind ethanolic extract on the number of leydig, sertoli, and spermatogenic cells of rat (rattus novergicus) exposed to heat Pratama, Abrian Panggalih Indra; Susilowati, Suherni; Maslachah, Lilik; Ratnani, Hermin; Suprayogi, Tri Wahyu
Ovozoa: Journal of Animal Reproduction Vol. 10 No. 1 (2021): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v10i1.2021.7-11

High temperatures lead to oxidative stress, which can disturb spermatogenesis process. Watermelon (Citrullus lanatus) peel contain antioxidant expected to compensate oxidative stress due to heat stress exposure. This study aimed to determine the effect of watermelon rind ethanolic extract on the number of Leydig, Sertoli, and spermatogenic cells of rats exposed to heat (40°C). Twenty rats (Rattus norvegicus) were divided randomly into five groups. In the control group (T0) rats were not exposed to heat nor given the watermelon rind extract. T1, T2, T3, and T4 groups were exposed to heat for an hour daily and orally given placebo (1% Na CMC), 100, 200, and 400 mg/kg BW of watermelon rind extract (in 1% Na CMC). Rats were treated for 52 days, and sacrificed for the testicle collection. Hematoxylin-eosin stained histological slides were prepared for the examination of Leydig, Sertoli and spermatogenic cells. The results showed no significant difference (p >0.05) in the average number of Leydig cells in rats among groups. The number of Sertoli cells and spermatogenic cells of rats exposed to heat (T1) was lower than those of the normal rats (T0 group). The dose of watermelon rind ethanolic extract at 200 mg/kg BW (T3 group) and 400 mg/kg BW (T4) increased (p <0.05) the number of Sertoli and spermatogenic cells. It could be concluded that a dose of 400mg/kg BW of watermelon rind ethanolic extract maintained the number of Leydig cells, Sertoli cells, and spermatogenic cells of rats exposed to heat.

The addition of vitamin C in tris–egg yolk extender maintained Sapera goat semen quality in 5° C storage Pahlevy, Jolly Rifqi; Ratnani, Hermin; Restiadi, Tjuk Imam; Fikri, Faisal; Saputro, Amung Logam; Agustono, Bodhi
Ovozoa: Journal of Animal Reproduction Vol. 11 No. 1 (2022): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v11i1.2022.1-8

Goats are small ruminants that are reared by the rural community for financial income and nutrition. This study was aimed to determine the effect of vitamin C addition in tris–egg yolk extender on the lifespan of Sapera goat spermatozoa stored in 5o C. Semen was collected from 1.5 years old Sapera male goat. The ejaculates were diluted in Tris-egg yolk (T-EY) extender added with 0, 0.2, 0.3, and 0.4 g vitamin C/mL extender for T0, T1, T2, and T3 groups, respectively. The spermatozoa motility, viability, and morphological abnormality were assessed along with storage at 5° C. The result showed that spermatozoa motility was the highest (p <0.05) in T1 compared to other groups and qualified for AI use for up to 72 hours. In 24 hours storage, the spermatozoa viability was not significantly different (p >0.05) among the groups. The spermatozoa viability in the T1 group was the highest (p <0.05) among the groups along 48-96 hours of storage. The spermatozoa morphological abnormalities of the T1 group was the lowest (p <0.05) compared to other treatment groups in the range of 24-72 hour storage. In the control group (T0), the less than 5% spermatozoa morphological abnormalities (qualified for artificial insemination) were only in the 24 hours storage, while those of the T1 group were up to 72 hours. It could be concluded that the addition of 0.2 g vitamin C/100 mL T-EY extender maintained the quality of Sapera goat semen for 72 hours at 5°C.

Retained placenta in dairy cows living in an all-day cowshed rearing system Sayi Datur Rohmah; Hermin Ratnani; Sunaryo Hadi Warsito; Rimayanti Rimayanti; Sri Pantja Madyawati; Sri Mulyati; Abdullah Hasib
Ovozoa: Journal of Animal Reproduction Vol. 12 No. 2 (2023): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v12i2.2023.71-80

This study aimed to determine the prevalence and factors that influence the incidence of retained placenta in dairy cows reared under an all-day cowshed housing system at KUD Tani Wilis, Sendang District, Tulungagung. This study used 216 dairy cows, that had already calved, that had been identified to have medical and reproductive records for data exploration. Data collected included the sex and weight of calf, single or twin calves, age of the dam, cowshed structure (floor, puddles on the floor, gutter, distance of cowshed to waste disposal, type of floor and roof, and sunlight exposure), feed of the dam (forage, concentrate, mineral supplements), frequency of retained placenta cases on calving intervals and days open of the dams. The prevalence of retained placenta was presented descriptively. Factors related to the retained placenta and its effect on calving interval and days open were analyzed by Chi-square test using Statistical Product and Service Solution (SPSS) software version 20 for Windows. The result showed that the prevalence rate of retained placenta was 19.91% of all dairy cow populations in the district. Furthermore, the sex of the calf, age of the dam, sunlight exposure, and the quantity of feed (forage and concentrate) were related to the retained placenta (p <0.05). In addition, retained placenta increased along with extending of days open and calving interval. It could be concluded that the factors that influenced the prevalence of retained placenta were the sex of the calf, the age of the cow, the feed and concentrate, and the cowshed that is not facing the sun.

Co-Authors AA Sudharmawan, AA Abdul Samik, Abdul Abdullah Hasib Agustono, Bodhi Azura, Sarah Berliana, Erprinanda Galuh BUDI UTOMO Budiarto Budiarto Cindy Ercha Aulia Putri Dady Soegianto Nazar Dinul, Ahmad Rajul Erma Safitri Faisal Fikri Frida Aulya Arningdiah Hana Eliyani Hani Plumeriastuti Hanifah, Novi Fitria Hariadi, Mas'ud Harijani, Nenny Imam Mustofa IMAM MUSTOFA Iwan Sahrial Hamid Kadek Rachmawati Khoirunnisa, Itrah Lilik Maslachah Luqman, Epy Muhammad Mafruchati, Maslichah Masruro, Novia Alfi Meles, Dewa Ketut Mufasirin Muhammad Al-Syafiq bin Abdul Halim Mujahid Mujahid, Mujahid Nurhusien Yimer Nuurin Ajrin Karim Pahlevy, Jolly Rifqi Pramudita, Kautsar Pandu Prastiya, Ragil Angga Pratama, Abrian Panggalih Indra Pudji Srianto Rahmatullah, Aldin Akbar Ramadhani, Fifi Fauziah Rimayanti Rimayanti Rimayanti Rochmah Kurnijasanti Saputro, Amung Logam Sayi Datur Rohmah Setiawan Koesdarto Soepranianondo, Koesnoto Sofie Adi, Keren Jayanti Sri Hidanah Sri Mulyati Sri Mulyati Sri Pantja Madyawati Sri Pantja Madyawati, Sri Pantja Suherni Susilowati Susilowati, Suheni Suzanita Utama Tehupuring, Benjamin Chr Tita Damayanti Lestari Tjuk Imam Restiadi Tri Wahyu Suprayogi Warsito, Sunaryo Hadi Wibawati, Prima Ayu Widya Paramita Lokapirnasari Wurlina, W Yeni Dhamayanti Yulianna Puspitasari Zahrorona, Amira Halimah

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