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All Journal Jurnal Ilmiah Farmasi Pendidikan Biologi Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Current Biochemistry Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Keperawatan BSI Adi Widya : Jurnal Pengabdian Masyarakat Pharmasipha Martabe : Jurnal Pengabdian Kepada Masyarakat Jurnal Farmasi Indonesia Jurnal Abdimas Mandiri INTEGRITAS : Jurnal Pengabdian Community Empowerment Jurnal Farmasi Proceeding Biology Education Conference Prosiding University Research Colloquium Journal Prima Health Science Herbal Medicine Journal SWAGATI: Journal of Community Service Jurnal Sains dan Kesehatan Advance Sustainable Science, Engineering and Technology (ASSET) Sabar : Jurnal Pendidikan Agama Kristen dan Katolik Jurnal Hukum, Administrasi Publik dan Negara
Desi Purwaningsih
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Biosorption Copper (Cu) and Mercury (Hg) by Omphalina sp. using Batch, Rotary, Biotray, and Pack Bed Flow Methods Desi Purwaningsih; I Made Artika; Tri Panji; Suharyanto Suharyanto
Current Biochemistry Vol. 3 No. 1 (2016)
Publisher : IPB University

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Abstract

Heavy metal waste treatment often uses dangerous chemicals. Omphalina sp is a nonpathogenic fungi that can be used to reduce the harmful effects of waste treatment. The use of fungal biomass has advantages such as low operating costs, efficient, and high metal binding capacity, minimal sludge, metals can be recovered, biosorbent can be regenerated, raw materials available easily, can use inactivated microorganism, and does not require additional nutrients. In the present study optimization of the biomass utilization for waste water treatment was conducted by comparing batch, rotary, packbed flow, and biotray methods. Results showed that Omphalina sp can reduce mercury level up to 91.38% with rotary, 83.98% with biotray, 87.14% with pack bed flow, and 31.94% with batch methods respectively from initial Hg concentration of 3 ppm. Similarly, Omphalina sp can reduce copper level up to 23.58% with rotary, 22.66% with biotray, 10.53% with pack bed flow, 10.17% with batch methods respectively from initial Cu concentration of 100 ppm. Optimum absorption Hg and Cu occurs in the first one hour.
Uji Aktivitas Antibakteri Ekstrak Etanol Daun Suruhan (Peperomia pellucida L. Kunth) Terhadap Bakteri Pseudomonas aeruginosa ATCC 27853 Desi Purwaningsih; Destik Wulandari
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 5, No 1 (2020): February 2020
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v5i1.3077

Abstract

Pseudomonas aeruginosa ATCC 27833 merupakan bakteri patogen oportunistik penyebab infeksi nosocomial pada pasien septikemia, sistik fibrosis, luka bakar, dan infeksi luka yang dapat menyebabkan tingkat mortilitas tinggi. Bakteri P. aeruginosa dilaporkan mengalami resistensi terhadap beberapa macam antibiotik. Hal tersebut dapat mengakibatkan lamanya waktu penyembuhan dan meningkatkan resiko kematian. Salah satu upaya untuk mengatasi permasalahan ini ialah dengan mengambil jalan alternatif menggunakan obat-obatan alami berbahan dasar tumbuhan. Salah satu tumbuhan yang memiliki potensi sebagai antimikroba adalah suruhan (Peperomia pellucida L. Kunth). Tujuan penelitian ini adalah untuk mengetahui aktivitas ekstrak daun suruhan dalam membunuh bakteri P. aeruginosa. Metode uji antibakteri yang digunakan dalam penelitian ini adalah uji dilusi untuk mengetahui Konsentrasi Hambat Minimum (KHM) dan Konsentrasi Bunuh Minimum (KBM). Ekstrak suruhan dibuat dalam 9 seri konsentrasi yaitu 50, 25, 12,5, 6,25, 3,12, 1,56, 0,78, 0,39, dan 0,19%. Hasil uji menunjukkan nilai KHM yang tidak dapat ditentukan karena campuran ekstrak daun suruhan dan bakteri P. aeruginosa sangat keruh. Nilai KBM yang diperoleh dari uji aktivitas antibakteri ekstrak daun suruhan terhadap bakteri P. aeruginosa adalah sebesar 25%.
Biosorpsi Tembaga (Cu) dan Merkuri (Hg) oleh Omphalina sp menggunakan Metode Batch, Rotary, Biotray, dan Pack Bed Flow Desi Purwaningsih; Tri Panji; I Made Artika
Proceeding Biology Education Conference: Biology, Science, Enviromental, and Learning Vol 12, No 1 (2015): Prosiding Seminar Nasional XII Biologi
Publisher : Universitas Sebelas Maret

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Abstract

Penelitian ini bertujuan untuk menentukan metode yang paling efektif pada biosorpsi logam oleh Omphalina. Biomassa yang masih hidup dapat menyerap logam lebih banyak dari pada biomassa yang sudah mati, sehingga  perlu dilakukan optimalisasi penggunaan biomasa dengan membandingkan metode batch, Rotary, packbed flow, biotray. Penelitian dilakukan pada larutan Cu 100 dan 200 ppm, dan Hg 3 dan 5 ppm, dengan variasi waktu kontak 1, 3, 5, 7, dan 24 jam , masing-masing sampel diukur kandungan logamnya dengan menggunakan spektrofotometri serapan atom. Hasil penelitian menunjukkan bahwa Hg dengan konsentrasi awal 3 ppm dapat berkurang hingga 91.38 % pada metode Rotary, 83.98% biotray, 87.14% pack bed flow, 32.94% batch, sedangkan pada Cu dengan konsentrasi awal 100 ppm dapat berkurang hingga 23.58% pada metode Rotary, 22.66%. biotray, 10.53% pack bed flow, dan 10.17% pada batch. Penyerapan optimal Hg dan Cu terjadi pada 1 jam pertama dan kapasitas serapan logam lebih tinggi pada metode rotary.Keywords: biosorpsi, merkuri, Omphalina sp, tembaga
UJI AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL DAUN SURUHAN (Peperomia pellucida L. Kunth) TERHADAP BAKTERI Shigella dysentriae Destik Wulandari; Desi Purwaningsih
Jurnal Farmasi Indonesia Vol 13 No 2 (2016): Jurnal Farmasi Indonesia
Publisher : Fakultas Farmasi Universitas Setia Budi

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (641.091 KB) | DOI: 10.31001/jfi.v13i2.288

Abstract

Shigella dysentriae is a bacteria that causes bacillary dysentery. This disease cause the patient to experience severe diarrhea. The efforts to overcome this disease is with using of antibiotics, but if the antibiotic is used for a long time can also to provide negative effects for humans. Therefore, needs a new antimicrobials that don't have negative effects for human, that is used of antimicrobial from plants. One of the plants that become candidate is suruhan (Peperomia pellucida L. Kunth). The purpose of this research is to know the antibacterial activity from the etanolic extract of the suruhan leaf (Peperomia pellucida L. Kunth) to against Shigella dysentriae bacteria by using dilution method. The method of this research is begins with the making of ethanol extract from suruhan leaf by maseration technique. The extracts then tested with concentrations of 100%, 80%, 60%, 40% and 20%. The antibacterial test using dilution method to find out Minimum Inhibition Concentration (MIC) and Minimum Kill Concentration (MKC). The results is show that MIC values can’t be determined because the mixture between the leaf extract and the Shigella dysentriae bacteria is very turbid. The value of MKC obtained by antibacterial activity from etanolic extract of the suruhan leaf on Shigella dysentriae bacteria was 40%.
Aktivitas Ekstrak Kasar Enzim Fibrinolitik Bakteri Bacillus cereus yang Diisolasi dari Air Hutan Mangrove Maroon Edupark Semarang secara In Vitro Rizky Bimantara HA; Ana Indrayati; Desi Purwaningsih
Jurnal Farmasi Indonesia Vol 19 No 1 (2022): Jurnal Farmasi Indonesia
Publisher : Fakultas Farmasi Universitas Setia Budi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31001/jfi.v19i1.1322

Abstract

Fibrinolytic enzymes are enzymes that work by degrading fibrin in blood clots. Fibrinolytic enzymes are produced by many organisms, one of which is the bacterium Bacillus cereus from the water of the Maroon Edupark mangrove forest, Semarang. Isolation of fibrinolytic enzymes from bacteria is very important because bacteria are easy to grow, have a fast generation time, do not require a large area for cultivation, and are easily genetically modified so that it will be more economically profitable. In addition, fibrinolytic enzymes from natural ingredients have fewer side effects than synthetic fibrinolytic drugs. This study aims to determine the activity of the crude extract of the fibrinolytic enzyme B. cereus in lysis blood clots in vitro. The study began with confirmation of the presence of genes encoding fibrinolytic enzymes from bacteria B. cereus uses NCBI's popular resources 'nucleotide', identification of bacterial morphology in blood agar media, Gram staining, endospore staining, catalase and coagulase testing. Isolation of crude extract of fibrinolytic enzyme B. cereus is carried out by enzyme extraction. Protein concentration were determined using Bradford method and fibrinolytic activity test in vitro using fibrin plate media with nattokinase as positive control. The resulting clear zone shows the ability of the enzyme extract in degrading fibrin The results of the identification of the B. cereus bacterial gene uses the NCBI data base registered as the AprE gene. The results of the identification of gram and endospore staining, B. cereus is a Gram-positive bacterium and has endospores. Identification of bacteria on blood agar media indicates that B. cereus represents the group of the β-hemolysis. Catalase and coagulase test results show that the bacteria produce catalase and coagulase enzymes. Total protein concentration from crude extract of B. cereus obtained at 19.63 mg/mL. Fibrinolytic activity at concentrations of 20, 40, 80% was 2.54; 6.11; and 7.94 mm respectively. Based on the above results it can be concluded that the crude extract of fibrinolytic enzyme B. cereus has the potential to be developed as a natural fibrinolytic agent.
Pelatihan Pembuatan Balsam Stik Dari Bahan Herbal Dan Perintisan Sebagai Home Industri dwi - Ningsih; Sri Rejeki Handayani; Ismi - Rahmawati; Dewi - Ekowati; Desi - Purwaningsih
Adi Widya : Jurnal Pengabdian Masyarakat Vol 3 No 1 (2019): ADIWIDYA
Publisher : Lembaga Penelitian dan Pengabdian Masyarakat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33061/awpm.v3i1.1979

Abstract

Balm, a pharmaceutical product that is no stranger to the community, therefore, this product is one of the most potential commodities developed into a home industry that can improve the economy of the community. relatively simple balm formulation and easy in material, is very possible to be applied in the community. This article is a form of publication of community service that has been carried out on Tuesday, February 6, 2018,at RW XXII Bibis Luhur Kel. Nusukan Kec. Banjarsari Surakarta Central Java. This community service is entitled "training of sticks from herbal and pioneering industry as a home industry" which aims to provide understanding and skill through counseling, training, and application of herbal balsam in the form of sticks, and utilization in health by empowering natural resources optimally, produce new products, besides can be made for self-use, sticks balm is a product that is very much needed by the community, so it is possible to develop its production in the scale of home industry to improve the economy of the community.
PKM PENGADAAN ALAT DESTILASI DI TUGUREJO RT 003 /RW 003, DESA SUKOREJO, KEC.MUSUK, KAB.BOYOLALI SEBAGAI UPAYA PENINGKATAN PEMANFAATAN LIMBAH DAUN CENGKEH Fitri Kurniasari & Desi Purwaningsih
Adi Widya : Jurnal Pengabdian Masyarakat Vol 2 No 2 (2018): ADIWIDYA
Publisher : Lembaga Penelitian dan Pengabdian Masyarakat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33061/awpm.v2i2.2499

Abstract

Cengkeh merupakan salah satu tanaman yang menjadi sumber alternatif dalam mendapatkan minyak atsiri dan memiliki berbagai macam khasiat untuk kesehatan. Kadar terbanyak dan kualitas yang baik dapat dihasilkan oleh minyak dari bagian bunga dan dahan/tangkai cengkeh. Daun cengkeh memiliki kandungan minyak atsiri 1-4% dan mudah diperoleh Dengan kandungan tersebut memungkinkan untuk dilakukan penyulingan minyak atsiri didalamnya, sehingga daun cengkeh yang sering dianggap limbah memiliki nilai ekonomis tinggi. Kadar eugenol minyak atsiri pada daun cengkeh sekitar 80-85%. Desa Musuk, Boyolali, Jawa tengah merupakan salah satu desa yang hampir semua penduduknya memiliki tanaman cengkeh, namun hasilnya kurang dimanfaatkan secara optimal. Penduduk sekitar lebih aktif berperan sebagai produsen bunga cengkeh dengan menjualnya ke pasar atau ke pengepul, sedangkan daun cengkeh jarang dimanfaatkan. Tujuan dari PKM ini adalah memberikan tambahan pengetahuan, ketrampilan dan peningkatan di bidang IPTEK untuk memaksimalkan manfaat limbah daun cengkeh dalam menghasilkan minyak atsiri yang berdaya jual tinggi dengan pengadaan alat destilasi. Metode yang digunakan adalah pendekatan kepada kedua mitra kerjasama yaitu mitra Tani “Berkah Jaya” dalam PKM ini akan berkontribusi sebagai produsen minyak atsiri dengan cara destilasi dari limbah daun cengkeh dan mitra “Cahaya Usaha” sebagai distributor /pemasok minyak atsiri dari limbah daun cengkeh.Kata kunci: Limbah Daun Cengkeh, Minyak atsiri, Mesin destilasi
PEMANFAATAN BEBERAPA TANAMAN HERBAL SEBAGAI ZAT AKTIF DALAM BEBERAPA SEDIAAN PENGHARUM RUANGAN SEBAGAI PENGUSIR NYAMUK Isna Jati Asiyah; Desi Purwaningsih; Destik Wulandari
INTEGRITAS : Jurnal Pengabdian Vol 5 No 1 (2021): JULI
Publisher : Relawan Jurnal Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36841/integritas.v5i1.818

Abstract

Nyamuk merupakan salah satu penyebab permasalahan masyarakat karena dapat menimbulkan berbagai penyakit dan mengganggu kenyamanan. Nyamuk dapat ditimbulkan karena kurangnya kebersihan lingkungan di masyarakat. Sekarang ini banyak masyarakat yang takut untuk menggunakan produk penolak nyamuk bentuk jadi yang telah dibuat dan dikeluarkan oleh pabrik atau industri tertentu. Kandungan kimia yang terkandung didalam sediaan tersebut menjadi alasan mengapa masyarakat mencari alternatif lain untuk membuat penolak nyamuk dengan menggunakan bahan alami yang lebih aman. Salah satu usaha pencegahan dan pengendalian terhadap serangan nyamuk yaitu dengan menggunakan pengharum ruangan dalam bentuk sediaan gel maupun lilin yang sekaligus dapat digunakan untuk pengusir nyamuk. Sediaan gel dan lilin pengharum ruangan pembuatanya cukup mudah dan bahan-bahan juga mudah didapatkan sehingga dapat dibuat sendiri oleh masyarakat. Keuntungan yang diperoleh masyarakat dengan pembuatan gel dan lilin pengharum ruangan secara mandiri yaitu aroma dapat disesuaikan dengan yang diinginkan, selain itu juga dapat dikembangkan menjadi home industry. Tujuan kegiatan ini adalah meningkatkan kesadaran masyarakat terhadap pemahaman pentingnya menjaga kebersihan lingkungan untuk menciptakan kesehatan pada keluarga serta melatih usaha pembuatan gel dan lilin aromaterapi dengan bahan alami, menghasilkan produk yang dapat digunakan sendiri maupun dikembangkan menjadi home industry. Kegiatan ini dilakukan dengan cara penyuluhan kebersihan lingkungan dan workshop pembuatan gel dan lilin aromaterapi sebagai alternatif pengusir nyamuk. Hasil yang diperoleh yaitu peningkatan pemahaman masyarakat terhadap kebersihan lingkungan, dihasilkan produk gel dan lilin aromaterapi sebagai pengusir nyamuk yang produksinya dapat dikembangkan dalam skala industri rumah tangga masyarakat Bibis Luhur RW 22 Nusukan.
Aktivitas Antibakteri Kombinasi Ekstrak Etanol Daun Johar (Cassia siemea Lamk) dan Daun Alpukat (Persea americana Mill) terhadap Streptococcus mutans Taufik Turahman; Desi Purwaningsih
Pharmasipha : Pharmaceutical Journal of Islamic Pharmacy Vol 6, No 2 (2022): September
Publisher : University Of Darussalam Gontor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21111/pharmasipha.v6i2.8693

Abstract

Dental caries is a dental infection with a high prevalence in Indonesia, one of the causes is Streptococcus mutans which demineralizes the tissues causing dissolution and dental caries. To reduce the number of microorganisms, people can utilize natural materials. One of the plants that have potential as an antibacterial is johar leaf and avocado leaf. The purpose of this study was to determine the antibacterial activity of the combination of ethanol extract of johar leaves (Cassia siamea Lamk) and avocado leaves (Persea americana Mill.) against Streptococcus mutans. This study uses the diffusion method. Extraction of johar leaves and avocado leaves was carried out by maceration method then the extracts were combined 1:1 with concentration series of 25%, 50%, 75% and 100%. Antibacterial testing uses the diffusion method to determine the inhibition. Streptococcus mutans bacteria were cultured at a density of 108 CFU/ml in MHA (Muller Hinton Agar) medium. Inhibitory power is known by measuring the diameter of the inhibition zone formed around the well. The average inhibition test results were obtained at concentrations of 25% (6 mm), 50% (6.1 mm), 75% (7.1 mm) and 100% (9.3 mm). The positive control of ciprofloxacin (30 mm) and the negative control did not show the diameter of the inhibition zone. So that the greater the concentration of the extract combination, the greater the diameter of the inhibition zone obtained to inhibit the growth of Streptococcus mutans and there is a significant difference in the diameter of the inhibition zone of the combination of extracts and ciprofloxacin positive control.
Potensi Senyawa Antibakteri Hasil Fermentasi Bakteri Endofit Dari Umbi Talas (Colocasia esculenta L.) Aji Wahyu Wahiddatul Djakiah; Ayu Rachmawati; Desi Purwaningsih; Destik Wulandari
Journal Prima Health Science Vol 1 No 1 (2019): Oktober
Publisher : LPPM STIKes Prima Indonesia

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Abstract

Endophytic bacteria are symbiotic microorganisms that live in plant tissues and an alternative producer of bioactive compounds that have the potential to be antibacterial. The study aims to determine the antibacterial activity of the bacteria Pseudomonas knackmussii and Bacillus siamensis in inhibiting the growth of Staphylococcus aureus ATCC 25923 and Escherichia coli ATCC 25922 as well as knowing the optimum time of fermentation of endophytic bacteria with the greatest antibacterial activity. The study was conducted in several stages, namely the identification of pathogenic bacteria and endophytic bacteria, fermentation of endophytic bacteria and antibacterial activity tests. Some treatment groups were ciprofloxacin 5μg as a positive control, sterile paper with sterile Brain-Heart Infusion (BHI) media as negative control andendophytic bacterial fermentation supernatant for 4 days as a test group. The results showed endophytic bacteria from taro tubers had antibacterial activity against S. aureus ATCC 25923 and E. coli ATCC 25922. The optimum time of fermentation with the largest antibacterial activity against S. aureus was on the 3rd day with inhibitory value of 9.69 mm produced by P. knakcmussii and 9,20 mm by B. siamensis while for E. coli the optimum time to ferment P. knackmussii is on the 2nd day with inhibitory values of 11.83 mm and B. siamensis on the 4th day of 10.78 mm.
Co-Authors Aji Wahyu Wahiddatul Djakiah Ana Indrayati Aris Tri Jaka Harjanta, Aris Tri Ayu anindia Ayu Rachmawati Bambang Agus Herlambang, Bambang Bowo Sugiharto Budi, Ikhsan Destik Wulandari Dewi Ekowati Dwi Ningsih Erna Irawan, Erna Febrian Murti Dewanto Ferina Dyah Prastiyanti Fitriana Noor Khayati Fradine, Chossy Harlita Harlita Haryo Kusumo Heryani, Monalisa I MADE ARTIKA I Made Artika Irawan, Ira Ismi Rahmawati Ismi Rahmawati Isna Jati Asiyah Juliana, Dara Kristina Simanjuntak, Kristina Simanjuntak Kusumo, Haryo Maharani, Novita Mandasari, Mitha Oktavia Marlina, Dian Mega Novita Mega Novita Ningsih, dwi - Nisa, Zaitun Pradana, Arsita Pradana, Arsitya Pratami Djasfar, Seftiwan Putra Djonesti, wilfadri Rahmawati, Ismi Rahmawati, Ismi - Reny Pratiwi Reny Pratiwi Retno Yulianti Rizky Bimantara HA Ryan Werytama Saputra Saputra, Ryan Werytama Setyaningsih, Widiastuti Simanjuntak, Kristina Siti Aisiyah Sri Hayati, Sri Sri Rejeki Handayani Sri Rejeki Handayani, Sri Rejeki Suharyanto Suharyanto Supriyono Supriyono Supriyono Supriyono Tanzila Rahmi Tita Novarini Tiwuk Susantiningsih Tri Panji Turahman, Taufik Turahman, Taufik Vicky Agus Arwanda Widiastuti Setyaningsih Wulandari, Destik ‪Arlina Dhian Sulistyowati‬