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Journal : Journal of Fisheries

Effect of Different Papain Concentrations on Yield and Quality of Tuna Eye Oil Trilaksani, Wini; Nurhayati, Tati; Santoso, Joko; Riyanto, Bambang; Fauziah, Silva; Sinulingga, Fahri
Jurnal Ilmiah Perikanan dan Kelautan Vol. 17 No. 2 (2025): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v17i2.57637

Abstract

Graphical Abstract Highlight Research Innovative Use of By-Products: The study explores the use of tuna eye by-products, a rich source of docosahexaenoic acid (DHA), to reduce reliance on imported fish oil and promote sustainable utilization of fishery waste. Enhanced Extraction Methodology: Enzymatic extraction using papain at optimal concentrations significantly improves the yield and quality of tuna eye oil, achieving up to six times higher yield compared to other methods. Nutritional and Quality Benefits: The extracted oil demonstrated low Index of Atherogenicity (IA) and Index of Thrombogenicity (IT), indicating its potential as a heart-healthy dietary supplement, with DHA and EPA concentrations well preserved. Environmental and Safety Advantages: The enzymatic process is solvent-free, minimizing environmental impact and ensuring consumer safety, while also addressing challenges posed by extended sample storage during the COVID-19 pandemic. Abstract Docosahexaenoic acid (DHA), a crucial omega-3 fatty acid, plays a vital role in neurodevelopment and cardiovascular health. Indonesia relies heavily on imported fish oil, despite its significant potential in underutilized by-products like tuna eyes. This study investigates the optimization of papain enzyme concentration for enzymatic extraction of DHA-rich oil from tuna eyes, aiming to enhance yield and maintain quality. Using 1% papain at 55°C for 1 hour, the optimized process achieved an oil yield of 8.59 ± 0.69%, six times higher than cold extraction without enzymes. The extracted oil exhibited high oxidative stability with low Index of Atherogenicity (IA: 0.38–0.40) and Index of Thrombogenicity (IT: 0.20–0.21), while DHA content remained well-preserved at 27.82%. This method also demonstrated the capability to maintain oil quality even after prolonged storage during the COVID-19 pandemic. Compared to conventional methods, enzymatic extraction provides a sustainable and efficient alternative by reducing chemical solvent use, minimizing environmental impact, and maximizing the utilization of fishery by-products. These findings offer a scalable solution for producing high-value omega-3 oils, contributing to global dietary needs and promoting sustainability in the fishery industry.
Co-Authors -, Nurseha A.A. Ketut Agung Cahyawan W Agung Budiwirawan Agus Taufik Mulyono Ahmad Harisb januar Syahidan, Ahmad Harisb januar Al-Faruqi, Muhammad Umar Alfa Narendra Alfila, Sinta Amelia Kusuma Indriastuti Ani Setiarini Arafah, Purnama Ariska, Risti Arivai Santosae, Zacky Astri Purnama Dewi Azhar Hermawan Riyanto, Azhar Hermawan Bagus Hario Setiadji Bambang Haryadi Bambang Pudjianto Bambang Susantono Cahyuning Isnaini, Cahyuning Dalimunthe, Zahirada Danang Parikesit Dardanila, Dardanila Darmawijoyo, Darmawijoyo Dendy Wicaksono Dianita Ratna Kusumastuti Dinamella Wahjuningrum Doni Ramadhan, Doni Dwi Widayati, Dwi Elisati Hulu Fauziah, Silva Ferry Hermawan Fitria, Hadaya Gugup Kismono Hidayati, Aulia Hinggo S, Hichmaed Tachta Indah, Febriamitha Indrasurya Budisatrya Mochtar Ismiyati Ismiyati Jogiyanto Hartono Joko Santoso Joko Siswanto Juang Akbardin Kami Hari Basuki Kami Hari Basuki Kustiariyah Tarman Lutfi, Agus Marsella, Emma Masrianto Masrianto Mudjiastuti Handajani Muhammad Amin Cakrawijaya Mustafid Mustafid Mustika Mahamarendra Mutik Sirait, Raisyicha Naibaho, Margareta Desnawati Nanda Dwi Astri Novita, Winda Duwi Nur Listiawati, Nur Nurhayati Harahap Nuroji Nuroji Poernomosidhi Poerwo Pratiwi, Ghina Eka Prestika Fiprilia Medawati Rakhmatika, Rakhmatika Ramadan Ramadan Ratu Ilma Indra Putri Reza Maulana Ria Asih Ariyani Soemitro Risti Ariska Ritonga, Parlaungan Rizky Akbar Fathurochman Rusdy A. Siroj S, Marsel Saulina Santosae, Zacky Arivai Simon Sili Sabon, Simon Sili Sinulingga, Fahri Sipayung, Klariani Sriningsih Sirait, Raisyicha Mutik Siswantari Sodikin Sodikin Soegiono Soetomo Sri Widyantoro, Sri Subijanto Sugeng Heri Suseno Syafrudin Syafrudin Syahfitri, Utami Dyah TATI NURHAYATI Tiftazani, M. Haekal Trilaksini, Wini Wahyu Ramadhan Wardhana A.S, Wisnu Wibowo, Slamet Widiarsyah, Farel Wini - Trilaksani Wirda, Yendri Wulandari, Ayu Septi Y I Wicaksono YI. Wicaksono Zulkardi Zulkardi Zulkardi