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All Journal HAYATI Journal of Biosciences Jurnal Hortikultura Indonesia (JHI) Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Bumi Lestari Zuriat Jurnal Ilmiah Aplikasi Isotop Radiasi BIOTROPIA - The Southeast Asian Journal of Tropical Biology AL KAUNIYAH Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Pelita Perkebunan AGRIVITA, Journal of Agricultural Science Jurnal Agroekoteknologi Indonesian Journal of Biotechnology Biology, Medicine, & Natural Product Chemistry Bioeksperimen: Jurnal Penelitian Biologi Journal of Tropical Biodiversity and Biotechnology ANNALES BOGORIENSES Jurnal Pertanian Agros Biosaintifika: Journal of Biology & Biology Education Scripta Biologica Jurnal Riset Biologi dan Aplikasinya JURNAL ILMIAH AGRINECA Agrosaintifika: Jurnal Ilmu-Ilmu Pertanian Annales Bogorienses
Endang Semiarti
Fakultas Biologi, Universitas Gadjah Mada, Indonesia
Aerospace Engineering Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Decision Sciences, Operations Research & Management Earth & Planetary Sciences Education Energy Environmental Science Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Public Health Veterinary

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Journal : Bumi Lestari

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KONSERVASI ANGGREK ALAM INDONESIA Vanda tricolor Lindl. varietas suavis MELALUI KULTUR EMBRIO SECARA IN-VITRO Rindang Dwiyani; Azis Purwantoro; Ari Indrianto; Endang Semiarti
Bumi Lestari Journal of Environment Vol 12 No 1 (2012)
Publisher : Environmental Research Center (PPLH) of Udayana University

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Abstract

Vanda tricolor Lindl. var. suavis is an Indonesian wild orchid which is now extremely rare in nature due to its habitat destruction. Development of an appropriate method for propagation of this species through in vitro culture could be nessecary for conservation purposes. High phenolic content of plant tissue is a serious problem for V. tricolor research in the laboratory, which inhibits germination and growth of the embryo. To overcome this problem, seeds were sown in a medium with the addition of tomato extract as an antioxidant. The aim of this research is to find the most suitable concentration of tomato extract for germination and growth of the embryo of V. tricolor form Bali and Merapi in order to obtained healthy seedlings for conservation purposes. Orchid pods (7 months after polination) of V. tricolor Bali and Merapi were used as plant material. The treatment consisted of 5 concentrations of tomato extract which is 0, 50 100, 150, 200, 250 g L-1. Observation was done by counting the number of protocorms for each stage of growth at 4 weeks after seed sowing. The study concluded that V. tricolor Bali is more responsive to the tomato extract compared with Merapi. Concentration of 150 gL-1 tomato extract gave the highest percentage of protocorms for Bali form, whereas Merapi form did not give significant differences either with or without tomato extract added in the culture medium.
Co-Authors , Surifah A.A. Ketut Agung Cahyawan W Achmadi Priyatmojo Aditya Nur Subchan Agus Slamet Ahmad Suyoko Ainina, Ana Alisa Julia Nurulita Alydarafa, Hafshah Aminatun Munawarti Aminatun Munawarti, Aminatun Amru Rizal Basri Anami Riastri Andani Kesuma, Amarilis Annisa, RR Rifka Ari Indrianto Ari Indrianto Ari Indrianto Ari Indrianto Ari Indrianto Ari Indrianto Ari Indrianto Aries Bagus Sasongko Arimarsetiowati, Rina Arum, Dyah Ayu Puspita Asri Fajar Milasari Azis Purwantoro Azis Purwantoro Aziz Purwantoro Bekti Sulistya Utami Binti Tsulsiyah Brilliant Kharisma Apritadila BUDI SETIADI DARYONO Cahya Lembayung Sutra Catharina Tri Widyastuti Christanti Sumardiyono Christanti Sumardiyono Della Rosiana Ningtias Devi Bunga Pagalla Dewi Tika Sari Dewi Yuliana Rizqi Dika Sundari Eka Fitriana Candra Ningrum El Hakim, Alim Erwin Prastowo Evilili Usmanti Exsyupransia Mursyanti Exsyupransia Mursyanti Exsyupransia Mursyanti Faiza Senja Widya Perdana Falah Nur Alifianto Febri Yuda Kurniawan Fhea Putri Cristy Fitriana Puspitasari Frisca Damayanti Gde Cahyadi Wirajagat Gildantia, Elke Hanifa, Yumna Rahmadias Ikhsanudin Nur Rosyidi Ixora Sartika Mercuriani JAKA WIDADA Jose Gutierrez-Marcos Jose Gutierrez-Marcos Kana Ninomiya Kinasih, Anggiresti Kurniawan, Febri Yuda Linggabuwana, Aviesta Maryani Maryani Matsumoto, Shogo Maura Indria Meidianing Meidianing, Maura Indria Melati, Chrisnanda Ayu Muhammad Dylan Lawrie Muhammad Dylan Lawrie Muhammad Dylan Lawrie Muhammad Dylan Lawrie Naufal Ghozi Aditya Perdana Ni Luh Putu Kayika Febryanti Nintya Setiari Nintya Setiari Nopitasari, Sri Oedjijono Oedjijono, Oedjijono Oktaviana Herawati Pauline Destinugrainy Kasi Prasojo, Ireneus Seno Pratiwi, Apriliana Putra, Rinaldi Rizal Putri, Saifa Usni Putu Ayu Erninda Oktaviani Suputri, Ni R Soelistijono Rahayu Sulistianingsih Ramadhani, Aulia Noor Rasjid, Nuzlan Rinaldi Rizal Putra, Rinaldi Rizal Rindang Dwiyani Rizka Riliant Puspasari Rumiyati Rumiyati Rumiyati, Rumiyati Seonghoe Jang Setiaji, Arkan Shogo Matsumoto Sismindari . Sismindari Sismindari Sismindari Sismindari Sitarina Widyarani Soenghoe Jang Sri Nopitasari Sri Wahyuningsih Stalis Norma Ethica Sukarti Moeljopawiro Sukarti Moeljopawiro Sukarti Moeljopawiro Sukarti Moeljopawiro Sukarti Moeljopawiro Sukarti Moeljopawiro Sukarti Moeljopawiro Sulastri Isminingsih Taryono, Taryono Thoyibatul Farida Tri Joko Raharjo Triono Bagus Saputro, Triono Bagus Umi Kulsum Nur Qomariah Windi Mose Windi Mose Woerjono Mangoendidjojo Woro Anindito Sri Tunjung Yasushi Yoshioka Yohana Theresia Maria Astuti, Yohana Theresia Maria Yoshioka, Yasushi Yudis Mahardhika, Ahmad Yuli Setiawati Yuuki Asano Zulfa Layina