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Fabrication of Calcite Calcium Carbonate from Eggshells Biogenic Waste Through Carbonation Method Mariyam, Mariyam; Sunarintyas, Siti; Yuliatun, Leny; Khuzaimah, Ismi; Nuryono, Nuryono
Jurnal Kimia Valensi Jurnal Kimia VALENSI, Volume 11, No. 1, May 2025
Publisher : Department of Chemistry, Faculty of Science and Technology Syarif Hidayatullah Jakarta State Islamic University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/jkv.v11i1.41914

Calcium carbonate derived from eggshells has significant potential for use in drug delivery systems, pharmaceutical, food, catalyst, cement, and concrete industries. Although eggshell waste is non-toxic, its excessive accumulation in the environment may contribute to ecological issues. Colonizing pathogenic bacteria in unprocessed eggshell waste poses a potential health risk. The present study outlines the production of precipitated calcium carbonate (PCC) from eggshell waste through a calcination-carbonation process, offering a sustainable approach to its utilization. The calcination was carried out at 900 ºC. The carbonation process was performed in an HNO3 solution, and the NH3 solution was under-treated for 60 minutes. The results exhibited that the precipitated calcium carbonate (PCC) had a purity of 95.2% CaO. It possessed predominantly the calcite phase with a rhombohedral crystal system, as confirmed by the XRD analysis. The crystallite size of PCC was 109.5 nm, measured using the Debye-Scherrer equation. The phase composition of PCC was 99.3% calcite, 0.5% vaterite, and 0.2% aragonite. FTIR analysis further corroborated this data by showing a sharp and unsplit peak at 1419 cm-1, demonstrating the presence of a calcite phase. SEM images revealed a cubic-like morphology, a characteristic of the calcite form of calcium carbonate (CaCO3). The synthesized calcium carbonate in this study holds potential for applications in dental materials and as fillers in polymer matrices for food packaging.

PEMERIKSAAN KOMPOSISI GLASS FIBER KOMERSIAL DENGAN TEKNIK X-RAY FLUORESCENCE SPECTROMETER (XRF) Sari, Widya Puspita; Sumantri, Dedi; Imam, Dian Noviyanti Agus; Sunarintyas, Siti
B-Dent: Jurnal Kedokteran Gigi Universitas Baiturrahmah Volume 1, Nomor 2, Desember 2014
Publisher : Universitas Baiturrahmah

Glass fiber lebih sering digunakan daripada polyethylene fiber sebagai komponen FRC dalam dunia kedokteran gigi. Glass fiber dental di Indonesia tersedia dalam jumlah terbatas dan memiliki harga yang relatif mahal. Tujuan dari penelitian ini adalah untuk memeriksa komposisi dari E-glass fiber dental dan glass fiber non dental menggunakan teknik XRF sehingga dapat ditentukan tipe dari masing-masing glass fiber non dental. Sampel terdiri dari 10 g E-glass fiber dental, 10 g fiberglass mats, 10 g fiberglass roving dan 10 g woven roving. Masing-masing sampel dihaluskan, selanjutnya dianalisa dengan dengan menggunakan X-Ray Fluorescence Spectrometer (XRF). Hasil analisa XRF sampel menunjukkan kandungan oksida terbesar pada E-glass fiber dental adalah SiO2 (45,47%), CaO (38,49%) dan Al2O3 (12,11%). Kandungan oksida terbesar pada fiberglass mats adalah SiO2 (56,88%), CaO (16,24%) dan Na2O (12,91%) demikian pula dengan woven roving yaitu SiO2 (55,86%), CaO (18,71%) dan Na2O (11,80%). Sedangkan fiberglass roving menunjukkan kandungan oksida terbesar antara lain SiO2 (52,56%), ZrO2 (14,64%) dan CaO (10,03%). Hal tersebut menunjukkan bahwa komposisi kandungan oksida pada sampel memiliki kemiripan dengan persentase yang berbeda. Berdasarkan pada analisis XRF disimpulkan bahwa glass fiber non dental jenis fiberglass mats dan woven roving mengarah ke tipe C-glass dan fiberglass roving mengarah ke tipe AR-glass.

The effect of casein and lactoferrin of bovine's milk on nestin expression of the exposed dental pulp Ningtyas, Endah Ariyati Eko; Widjijono, Widjijono; Sunarintyas, Siti
Odonto : Dental Journal Vol 9, No 2 (2022): December 2022
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Background: Pulp tissue can regenerate if damage occurs. The pulp differentiates into new odontoblast-like cells in Progenitor cells. Nestin is a marker of the presence of new dontoblast-like and expressed in pathological conditions. Bovine's milk casein and lactoferrin contain proteins that accelerate cell growth. This study aimed to determine the effect of casein and lactoferrin on the expression of nested cells in the exposed dental pulp.Method: The research method is an experimental laboratory with male 30 Spraque Dawley rats. Rats were grouped as : casein, lactoferrin, casein-lactoferrin, and Ca-(OH)2. Each group consisted of 15 samples of molars which were divided into 5 samples of teeth for observation on the 7, 14, and 21 days. The first molars of rats were prepared until the pulp was exposed. The data of nestin quantity with immunohistochemical (IHC) analysis on histological preparations were analyzed using two-way ANOVA then the least significance difference (LSD) test.Result: The results of the average number of nested cells on 7,14 and 21 days were highest on combination of casein and lactoferrin. Two-way ANOVA statistical proved that there was a significant difference (p<0.05) in each observation period and group and the number of expressions of nestin cells. LSD test showed that there was a significant difference (p<0.05) between groups compared to the control group.Conclusion: Combination casein and lactoferrin extracts affect the expression of the number of nestin cells as a marker of new odontoblast-like cells in the exposed dental pulp.

ROLE OF COMBINATION CASEIN AND LACTOFERRIN BOVINE’S COLLOSTRUM AS A PULP CAPPING ON MACROPHAGE EXPRESSION IN MALE WISTAR RATS Ningtyas, Endah Ariyati Eko; Santoso, Oedijani; Sadhana, Udadi; Sunarintyas, Siti
Odonto : Dental Journal Vol 8, No 2 (2021): December 2021
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

ABSTRACTBackground: Inflammatory and/or non-inflammatory processes play a role in stimulating pulp repair and the formation of hard tissue, namely reparative dentin. Macrophages play a role in the pathogenesis and chronic inflammatory disorders. The combination casein lactoferrin of bovine colostrum as an immunomodulator has therapeutic potential. This study aims to determine the therapeutic effect and duration of application of the combination of casein lactoferrin of bovine colostrum, on the expression of macrophages as pulp capping.Method: This study was a true experimental laboratories post test only control group design, consisting of three groups of 60 male wistar rats with 4 observation times, namely day to day 7, 14, 21 and 28 each of 5 mice. The maxillary 1st molars were prepared until the roof of the pulp was exposed. Three groups, namely the combination of casein and lactoferrin bovine colostrum (CKL) and calcium hydroxide (K1) and the untreated group (K0). Each group was filled with glassionomer as a permanent restoration. The tissue was made histological preparations with hematoxylin-eosin staining and the number of macrophages were counted, then analyzed by two way ANOVA and post hoc LSD tests.Result: The results showed that the therapeutic effect and duration of application of the combination of casein and lactoferrin bovine colostrum on the expression of macrophages as pulp cappingConclusion: The combination of casein and lactoferrin of bovine colostrum as capping material can increase the number of macrophages in the healing process of dental pulp.

The effect of casein and lactoferrin of bovine's milk on nestin expression of the exposed dental pulp Ningtyas, Endah Ariyati Eko; Widjijono, Widjijono; Sunarintyas, Siti
Odonto : Dental Journal Vol 9, No 2 (2022): December 2022
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/odj.9.2.273-278

Background: Pulp tissue can regenerate if damage occurs. The pulp differentiates into new odontoblast-like cells in Progenitor cells. Nestin is a marker of the presence of new dontoblast-like and expressed in pathological conditions. Bovine's milk casein and lactoferrin contain proteins that accelerate cell growth. This study aimed to determine the effect of casein and lactoferrin on the expression of nested cells in the exposed dental pulp.Method: The research method is an experimental laboratory with male 30 Spraque Dawley rats. Rats were grouped as : casein, lactoferrin, casein-lactoferrin, and Ca-(OH)2. Each group consisted of 15 samples of molars which were divided into 5 samples of teeth for observation on the 7, 14, and 21 days. The first molars of rats were prepared until the pulp was exposed. The data of nestin quantity with immunohistochemical (IHC) analysis on histological preparations were analyzed using two-way ANOVA then the least significance difference (LSD) test.Result: The results of the average number of nested cells on 7,14 and 21 days were highest on combination of casein and lactoferrin. Two-way ANOVA statistical proved that there was a significant difference (p<0.05) in each observation period and group and the number of expressions of nestin cells. LSD test showed that there was a significant difference (p<0.05) between groups compared to the control group.Conclusion: Combination casein and lactoferrin extracts affect the expression of the number of nestin cells as a marker of new odontoblast-like cells in the exposed dental pulp.

ROLE OF COMBINATION CASEIN AND LACTOFERRIN BOVINE’S COLLOSTRUM AS A PULP CAPPING ON MACROPHAGE EXPRESSION IN MALE WISTAR RATS Ningtyas, Endah Ariyati Eko; Santoso, Oedijani; Sadhana, Udadi; Sunarintyas, Siti
Odonto : Dental Journal Vol 8, No 2 (2021): December 2021
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/odj.8.2.156-164

ABSTRACTBackground: Inflammatory and/or non-inflammatory processes play a role in stimulating pulp repair and the formation of hard tissue, namely reparative dentin. Macrophages play a role in the pathogenesis and chronic inflammatory disorders. The combination casein lactoferrin of bovine colostrum as an immunomodulator has therapeutic potential. This study aims to determine the therapeutic effect and duration of application of the combination of casein lactoferrin of bovine colostrum, on the expression of macrophages as pulp capping.Method: This study was a true experimental laboratories post test only control group design, consisting of three groups of 60 male wistar rats with 4 observation times, namely day to day 7, 14, 21 and 28 each of 5 mice. The maxillary 1st molars were prepared until the roof of the pulp was exposed. Three groups, namely the combination of casein and lactoferrin bovine colostrum (CKL) and calcium hydroxide (K1) and the untreated group (K0). Each group was filled with glassionomer as a permanent restoration. The tissue was made histological preparations with hematoxylin-eosin staining and the number of macrophages were counted, then analyzed by two way ANOVA and post hoc LSD tests.Result: The results showed that the therapeutic effect and duration of application of the combination of casein and lactoferrin bovine colostrum on the expression of macrophages as pulp cappingConclusion: The combination of casein and lactoferrin of bovine colostrum as capping material can increase the number of macrophages in the healing process of dental pulp.

EDUKASI PEMANFAATAN BUAH NAGA MERAH SEBAGAI BAHAN DISCLOSING AGENT PADA MASYARAKAT SRENGAT, BLITAR Dea Cahyani, Yessica; Khafid, Mohammad; Handajani, Juni; Hanindriyo, Lisdrianto; Irnawati, Dyah; Susilowati, Heni; Sunarintyas, Siti
Jurnal Pengabdian Pendidikan Masyarakat (JPPM) Vol 6 No 2 (2025): Jurnal Pengabdian Pendidikan Masyarakat (JPPM), Vol 6 No 2 (Oktober 2025)
Publisher : LPPM UNIVERSITAS MUHAMMADIYAH MUARA BUNGO

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52060/jppm.v6i2.2845

Using a disclosing agent to control plaque is one way to improve dental health and oral tissue. However, this material is not widely known to the public. Red dragon fruit, a mainstay commodity in Blitar Regency, contains betalain which can be used as an alternative disclosure agent. Therefore, the people of Blitar Regency need to be empowered to maintain dental and oral health regarding the potential of dragon fruit as an alternative natural ingredient. The aim is to provide education regarding the potential benefits of dragon fruit as a disclosure agent. The strategy used in this activity is dual media intervention by dentists, namely by using interactive lectures with PowerPoint media and booklet media to community groups in, Blitar Regency. To measure the level of knowledge of the target community, questionnaires were given before and after the activity, as well as checking the OHI-S index. The research results showed that the level of the OHI-S index was in the medium category, while the level of community knowledge showed an increase of 56.78% after being given counseling. This shows that providing dental and oral health education using PowerPoint media and booklet media has proven to be effective in increasing public knowledge. Therefore, it can be concluded that providing education on the use of red dragon fruit as a disclosing agent to the people of Blitar Regency has proven effective in increasing knowledge, so it is hoped that it can improve the community's dental and oral hygiene.

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