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Penentuan Kadar Rubraxanton pada Ekstrak Kulit Batang Garcinia spp Susanti, Meri; Dachriyanus, .; Putra, Deddy Prima; Sriwahyuni, Fatma
JFIOnline | Print ISSN 1412-1107 | e-ISSN 2355-696X Vol 6, No 3 (2013)
Publisher : Indonesian Research Gateway

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (323.063 KB)

Abstract

In this study a High Performance Liquid Chromatography (HPLC) has been used for determination of rubraxanton on bark extracts Garcinia spp (Garcinia mangostana, Garcinia Cowa, Garcinia griffitii, Garcinia dioica and Garcinia forbesii). HPLC system consisted of C-18 reversed phase column with a length of 250 mm, diameter 4.6 mm, 20 mL injection volume, mobile phase methanol: water (gradient system with polarity) and flow rate of 1ml/min. Rubraxanton levels obtained in this study; 9.161% for G.mangostana, 6.942% for G.cowa, 6.762% for G.dioica, 0.499% for G.forbesii and 0.229% for G.griffitii. The method has been validated for specificity, linearity, accuracy, precision, limits of detection (LOD) and limits of quantitation (LOQ). The linearity of the method can be seen from the regression coefficient r=0.9996 with a linearity range from 1.72 to 55 ug/ml. Recovery of rubraxanton in the extract of G.mangostana was between 99.61 to 101.08%. Intra and inter day precision showed relatively small level of standard deviation (lower than 2%). Limit Of Detection (LOD) and Limit Of Quantitation (LOQ) are 0.55 ug/ml and 1.82 ug/ml respectively.Keywords : rubraxanton, bark extracts, Garcinia spp.
Penentuan Kadar Rubraxanton pada Ekstrak Kulit Batang Garcinia spp Susanti, Meri; Dachriyanus, .; Putra, Deddy Prima; Sriwahyuni, Fatma
Jurnal Farmasi Indonesia Vol 6, No 3 (2013)
Publisher : Jurnal Farmasi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (323.063 KB) | DOI: 10.35617/jfi.v6i3.137

Abstract

In this study a High Performance Liquid Chromatography (HPLC) has been used for determination of rubraxanton on bark extracts Garcinia spp (Garcinia mangostana, Garcinia Cowa, Garcinia griffitii, Garcinia dioica and Garcinia forbesii). HPLC system consisted of C-18 reversed phase column with a length of 250 mm, diameter 4.6 mm, 20 mL injection volume, mobile phase methanol: water (gradient system with polarity) and flow rate of 1ml/min. Rubraxanton levels obtained in this study; 9.161% for G.mangostana, 6.942% for G.cowa, 6.762% for G.dioica, 0.499% for G.forbesii and 0.229% for G.griffitii. The method has been validated for specificity, linearity, accuracy, precision, limits of detection (LOD) and limits of quantitation (LOQ). The linearity of the method can be seen from the regression coefficient r=0.9996 with a linearity range from 1.72 to 55 ug/ml. Recovery of rubraxanton in the extract of G.mangostana was between 99.61 to 101.08%. Intra and inter day precision showed relatively small level of standard deviation (lower than 2%). Limit Of Detection (LOD) and Limit Of Quantitation (LOQ) are 0.55 ug/ml and 1.82 ug/ml respectively.Keywords : rubraxanton, bark extracts, Garcinia spp.
Co-Authors Akbar, Surya Amatul Firdausa Nasa Andar Indra Sastra Anggreiny, Nila Angraini, Atika Ledia Annisa Fitriani, Annisa Anshori, Putra Anugrah, Raffli Dwi Ar, Rosmita Ariandi, M. Satriadi Aulia, Said Reza Bangun, Mentari Wahyunta Br Br Saragih, Zanariah Busihat, Busihat Cikita Aidha Putri Dachriyanus Dania, Ira Aini Darni Darni Destiniar Destiniar, Destiniar Dewi Pangestuti Diny Amenike Dira Hefni Dwi Puspasari Dwisesaria Irma Lena Efrisnal, Dodi Egha Febrianingsih Erlinda Erlinda Fahmy, Rahmi Fatma Sri Wahyuni Fatsiwi Nunik Andari Fitria Rahmi Hariko, Rezki Haznaz Dhiya Ulhaq Z.N. Hildayani Husna, Muppidatul Ibarra, Florante Isna Mulyani Izzanil Hidayati Julianita, Diani Kurniawati kusmiarti, reni Lady Diana Yusri lala septiyani sembiring, lala septiyani Liliyana Sari Lina, Liza Fitri Lubis, Adi Raja Brando M. Yogi Riyantama Isjoni Mafaza Mafaza Mamnun, Hairo Manurung, Mei Juita Mardha Akhsanita Maria Goretti Adiyanti Mayang Sari Ayu Meifal Rusli Nasril, Nasril Nasution, Arifah Najiha Nasution, Halimah Thania Natalia, Nela Netrawati, Netrawati Neviyarni, Neviyarni Nila Anggreiny Nila Angreiny Ningtiyas, Meiga Puji Nita, Melda Nur Ahyani Nuzli, Muhammad Oktaria, Selly Putra, Deddy Prima Putra, Deddy Prima Qolbi Khoiri Rahma, Dinda Saufia Rangkuti, Irmayanti Rizqillah Pasaribu, Saadatur Rohmatin, Rohmatin Rozi Sastra Purna Sabiruddin, Sabiruddin Salsabila, Cantika Sari, Siti Kemala Sastra, Ervan Yuli Septi Mayang Sarry Shasitharan Raman Kutty Silvi Yulia Sari Siska Oktari Sufyarma Marsidin Suhatri Suhatri Surya Akbar Sylvia Dewiriza Tiarnida Nababan Tri Rahayuningsih Wahyu Widodo Wahyu, Haifa Yantri Maputra Yeni Karneli Yohannes Alen Yosea Kurnianto