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Journal : The Indonesian Journal of Dental Research

Micronucleus frequency of the buccal epithelial cells on pesticide-exposed female farmers in Dieng village, Central Java Yudasari, Innayati Oktiana; Susilowati, Heni; Jonarta, Alma Linggar
The Indonesian Journal of Dental Research Proceeding Book
Publisher : The Indonesian Journal of Dental Research

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Abstract

Pesticide is a toxic material used to eradicate physical intruder of crop, including pests, diseases, or weeds. Long-term exposure of pesticide to human body may give genotoxic effects at cellular level, as its substances are chemically bond with the components of DNA triggering the damage of DNA and chromosomes. Micronucleus, additional nucleus on the cells, presents as a result of the termination of the chemical bonds of DNA. The aim of this study was to investigate the frequency of micronuclei of buccal epithelial cells on pesticide-exposed female farmers in Dieng Village, Central Java. This research was conducted on 36 female villagers of Dieng Plateau. The first group consisted of 18 subjects who had regularly and directly exposed by pesticides due to their job as farmers. The control group consisted of 18 subjects were unexposed to pesticides. Buccal epithelium cells of all subjects was collected by swabing the right and left buccal mucosa using cytobrush, fixed and stained using Feulgen-Rossenbeck method. The micronuclei frequency was counted per 1000 epithelial cells. Data was analyzed using Independent Sample t-test. The statistical analysis showed a significant difference between exposed and unexposed groups (p= 0,01). It is concluded that there was an increase in the micronucleus frequency of buccal epithelial cells on pesticide-exposed female farmers in Dieng Village. Moreover, direct long-term exposure of pesticides may harm and damage the human body cells at the gene level.
Co-Authors Ady, Pangeran Pandu Bayu Agustinus Rudolf Phym, Agustinus Agustiya, Arvi Alma Linggar Jonarta Andriana, Myra Anne Handrini Dewi Aprilia, Sintya Asikin Nur Atsushi Tabata Ayun Febriana Azzahra, Annisa Annur Dea Cahyani, Yessica Denise Utami Putri, Denise Utami Devi Chretella Maheswara, Devi Chretella Dewi Agustina Dewi Widyaningsih Dyah Irnawati Dyah Listyarifah Edy Meiyanto Endah Kusumastuti Fahlevi, Bunga Farida, Dwi Ana Farmasyanti, Cendrawasih Andusyana Fery Setiawan Hanif, Hilmy Irsyadi Hardiyani, Reisca Tiara Hargyatni, Titin Hary Hermawan Hayuningtyas, Widya Galuh Henny Henny, Henny Heribertus Dedy Kusuma Yulianto Hermawan Prasetyanto HIDAYAT, ERWAN NUR Hideaki Nagamune Hirohiko Okamura Innayati Oktiana Yudasari, Innayati Oktiana Intan Ruspita, Intan Irsalina, Nadia Iswari, Febri Nandia Sri Jaelani, Jaelani Juni Handajani Katsuhiko Hirota Kaya Yoshida Kuijpers-Jagtman, Anne Marie Kustami, Endang Kusumaningsih, Dhevi Dadi Kusumaningtyas, Dhevi Dadi Lisdrianto Hanindriyo Mariana, Septanus Rita Maulana, Putri Anindya Mohammad Khafid Mu'tashim, Muhammad Faris Mun‘im, Abdul Nunuk Purwanti Pertiwi, Heppy Atma Prabowo, Adi Prakosa, Basma Rosandi Prawira, Anggun Wida Ratnaningrum Ratnaningrum, Ratnaningrum Retnosari Andrajati Sati, Dian Laras Selvy, Mutiara Septiani, Intan Dzahabiyah Shofiana, Umu Salma Siagian, JW Siti Sunarintyas Siwi, Dyanasti P. Sulistyowati, Pemilia Suryani Hutomo, Suryani Suryanto, Yanti Ivana Tatsuji Haneji Titin Hargyatni Trianna Wahyu Utami Wijayanti, Afifah Arum Wulandari Wulandari Yoga, Ika Yoichiro Miyake Yosaphat Bayu Rosanto Ziadah, Tsai'un Nur